Restriction map for pF1KA0584
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        AspEI       AspI        
AvrII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BfrI        BglII       BlnI        BsaAI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspLU11I    Bst1107I    
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1700  GGTACC               G^GTACC
AlwI         1195  GATCC                GGATC(4/5)
Asp700I      1641  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1700  GGTACC               G^GTACC
AvaI          584  CTCGGG               C^[CT]CG[GA]G
AviII        1079  TGCGCA               TGC^GCA
BanI         1700  GGTACC               G^G[CT][GA]CC
BanII         153  GAGCCC               G[GA]GC[CT]^C
BbvCI          50  CCTCAGC              CCTCAGC(-5/-2)
BclI          273  TGATCA               T^GATCA
BfaI         1285  CTAG                 C^TAG
BglI          256  GCCATCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1375  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1375  GCTCAGC              GC^T[ACGT]AGC
BsaBI         252  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1717  GAGACC               GGTCTC(1/5)
BsaWI        1070  ACCGGA               [AT]^CCGG[AT]
BsgI          934  GTGCAG               GTGCAG(16/14)
BsoBI         584  CTCGGG               C^[CT]CG[GA]G
BspHI        1034  TCATGA               T^CATGA
BspMI         872  ACCTGC               ACCTGC(4/8)
BsrFI         123  GCCGGT               [GA]^CCGG[CT]
BssSI         166  CTCGTG               CACGAG(-5/-1)
BstAPI        195  GCACACGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         455  TTCGAA               TT^CGAA
BstXI         397  CCAAAGCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1194  AGATCC               [GA]^GATC[CT]
CelII        1375  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        123  GCCGGT               [GA]^CCGG[CT]
DrdI          796  GACATCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         726  CCTCAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       698  CCATGG               C^C[AT][AT]GG
FbaI          273  TGATCA               T^GATCA
FspI         1079  TGCGCA               TGC^GCA
HincII        502  GTTGAC               GT[CT]^[GA]AC
HindII        502  GTTGAC               GT[CT]^[GA]AC
KpnI         1700  GGTACC               GGTAC^C
MaeI         1285  CTAG                 C^TAG
MaeIII       1709  GTGAC                ^GT[ACGT]AC
MamI          252  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1194  AGATCC               [GA]^GATC[CT]
MseI         1334  TTAA                 T^TAA
NcoI          698  CCATGG               C^CATGG
NspI         1770  ACATGC               [GA]CATG^[CT]
NspV          455  TTCGAA               TT^CGAA
RcaI         1034  TCATGA               T^CATGA
SexAI        1460  ACCTGGT              A^CC[AT]GGT
SfuI          455  TTCGAA               TT^CGAA
StyI          698  CCATGG               C^C[AT][AT]GG
Tru9I        1334  TTAA                 T^TAA
Tsp45I       1709  GTGAC                ^GT[GC]AC
XhoII        1194  AGATCC               [GA]^GATC[CT]
XmnI         1641  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI          622  CCTAAGG              CC^T[ACGT]AGG
              726  CCTCAGG             
Bsp1407I      324  TGTACA               T^GTACA
             1586  TGTACA              
BsrBI          94  GAGCGG               CCGCTC(-3/-3)
              226  GAGCGG              
BsrDI         946  GCAATG               GCAATG(2/0)
             1808  GCAATG              
BsrGI         324  TGTACA               T^GTACA
             1586  TGTACA              
BssHII          7  GCGCGC               G^CGCGC
               79  GCGCGC              
Bsu36I        622  CCTAAGG              CC^T[ACGT]AGG
              726  CCTCAGG             
DraIII        277  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
              340  CACTATGTG           
DsaI          158  CCACGG               C^C[GA][CT]GG
              698  CCATGG              
EarI          672  GAAGAG               CTCTTC(1/4)
             1410  GAAGAG              
Eco81I        622  CCTAAGG              CC^T[ACGT]AGG
              726  CCTCAGG             
HphI         1225  GGTGA                GGTGA(8/7)
              617  TCACC               
Ksp632I       672  GAAGAG               CTCTTC(1/4)
             1410  GAAGAG              
MaeII         653  ACGT                 A^CGT
             1499  ACGT                
MslI          834  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              927  CATCCATGTG          
MspA1I       1153  CAGCTG               C[AC]G^C[GT]G
             1378  CAGCTG              
PflMI        1724  CCACCATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1751  CCACCGACTGG         
PpuMI         640  AGGACCC              [GA]G^G[AT]CC[CT]
             1634  GGGACCT             
PvuII        1153  CAGCTG               CAG^CTG
             1378  CAGCTG              
SspBI         324  TGTACA               T^GTACA
             1586  TGTACA              
SspI          300  AATATT               AAT^ATT
             1168  AATATT              
Van91I       1724  CCACCATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1751  CCACCGACTGG         
XcmI          158  CCACGGTGCTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              242  CCAAGAGCAGGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BalI        BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MboI        MboII       MluNI       MnlI        
MscI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        TaqI        TfiI        
TseI        Tsp509I     TspRI       TthHB8I     
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