Restriction map for pF1KA0541
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AviII       AvrII       BbeI        BbrPI       BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsiWI       BspHI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       Cfr10I      CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       FseI        FspI        Hin1I       
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    RcaI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       Sse8387I    SwaI        XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII       4145  TCCGGA               T^CCGGA
AseI          160  ATTAAT               AT^TAAT
AsnI          160  ATTAAT               AT^TAAT
Asp700I      1834  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          167  CTCGAG               C^[CT]CG[GA]G
BamHI        3496  GGATCC               G^GATCC
BbsI         4034  GTCTTC               GAAGAC(2/6)
BbvCI        4315  CCTCAGC              CCTCAGC(-5/-2)
BglI         3076  GCCGCACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        4034  GTCTTC               GAAGAC(2/6)
BsaBI        2878  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1425  GGTCTC               GGTCTC(1/5)
BsaWI        4145  TCCGGA               [AT]^CCGG[AT]
BseAI        4145  TCCGGA               T^CCGGA
BsiEI          98  CGATCG               CG[GA][CT]^CG
BsmBI        1899  GAGACG               CGTCTC(1/5)
BsmI         2474  GAATGC               GAATGC(1/-1)
BsoBI         167  CTCGAG               C^[CT]CG[GA]G
BspDI        3053  ATCGAT               AT^CGAT
BspEI        4145  TCCGGA               T^CCGGA
BstAPI       3873  GCAGAATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI         3053  ATCGAT               AT^CGAT
DraIII       1376  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          122  GACAAATATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2784  CCTTTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1653  ATGCAT               ATGCA^T
HaeII          48  AGCGCC               [GA]GCGC^[CT]
HindIII       215  AAGCTT               A^AGCTT
MamI         2878  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         4145  TCCGGA               T^CCGGA
NsiI         1653  ATGCAT               ATGCA^T
PaeR7I        167  CTCGAG               C^TCGAG
PshBI         160  ATTAAT               AT^TAAT
PvuI           98  CGATCG               CGAT^CG
SacI         4112  GAGCTC               GAGCT^C
SapI         3268  GAAGAGC              GCTCTTC(1/4)
ScaI         2442  AGTACT               AGT^ACT
SpeI         3487  ACTAGT               A^CTAGT
XhoI          167  CTCGAG               C^TCGAG
XmnI         1834  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII        3210  CGCG                 CG^CG
             4362  CGCG                
AocI         3239  CCTCAGG              CC^T[ACGT]AGG
             3469  CCTGAGG             
BalI         1360  TGGCCA               TGG^CCA
             3512  TGGCCA              
BanI         4311  GGCACC               G^G[CT][GA]CC
             4344  GGTGCC              
BcgI         3678  CGAACTTTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3742  GCAGCTGACTCG        
BciVI         597  GGATAC               GTATCC(6/5)
             1767  GGATAC              
Bsp1407I      930  TGTACA               T^GTACA
             1238  TGTACA              
BspLU11I     1402  ACATGT               A^CATGT
             2064  ACATGT              
BsrGI         930  TGTACA               T^GTACA
             1238  TGTACA              
BssSI        3899  CACGAG               CACGAG(-5/-1)
             3973  CTCGTG              
BstUI        3210  CGCG                 CG^CG
             4362  CGCG                
Bsu36I       3239  CCTCAGG              CC^T[ACGT]AGG
             3469  CCTGAGG             
Eco81I       3239  CCTCAGG              CC^T[ACGT]AGG
             3469  CCTGAGG             
HgaI         2611  GCGTC                GACGC(5/10)
             4363  GCGTC               
MluNI        1360  TGGCCA               TGG^CCA
             3512  TGGCCA              
MscI         1360  TGGCCA               TGG^CCA
             3512  TGGCCA              
MvnI         3210  CGCG                 CG^CG
             4362  CGCG                
NcoI         4119  CCATGG               C^CATGG
             4170  CCATGG              
NdeI         2527  CATATG               CA^TATG
             2737  CATATG              
PflMI         704  CCAAATATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1246  CCAGCACATGG         
PpuMI         753  AGGTCCT              [GA]G^G[AT]CC[CT]
             2748  GGGTCCT             
PstI         4059  CTGCAG               CTGCA^G
             4193  CTGCAG              
SexAI        1721  ACCTGGT              A^CC[AT]GGT
             4340  ACCAGGT             
SmlI          167  CTCGAG               C^T[CT][GA]AG
             4387  CTCAAG              
SphI         3768  GCATGC               GCATG^C
             4298  GCATGC              
SspBI         930  TGTACA               T^GTACA
             1238  TGTACA              
StuI         1705  AGGCCT               AGG^CCT
             3013  AGGCCT              
Van91I        704  CCAAATATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1246  CCAGCACATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AspI        
AvaII       BanII       BbvI        BclI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoRV       EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XcmI        XhoII       
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