Restriction map for pF1KA0541
 (Linear) (Six-base) MAPSORT of: KIAA0541.seq  Check: 7357  from: 1  to: 4470



KIAA0541.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577    2132    3269    4470 
   Size:        577    1555    1137    1201

  Fragments arranged by size: 

                1555    1201    1137     577
 
AflIII A'CryG_T

Cuts at:      0    1402    2064    4274    4470 
   Size:       1402     662    2210     196

  Fragments arranged by size: 

                2210    1402     662     196
 
AleI CACnn'nnGTG

Cuts at:      0    4195    4470 
   Size:       4195     275
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4315    4470 
   Size:       4315     155
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2614    3087    3247    3745    4470 
   Size:       2614     473     160     498     725

  Fragments arranged by size: 

                2614     725     498     473     160
 
ApoI r'AATT_y

Cuts at:      0     307     915    1992    2154    3007    3335    3406    3464 
   Size:        307     608    1077     162     853     328      71      58

Cuts at:   3464    3914    3932    4470 
   Size:        450      18     538

  Fragments arranged by size: 

                1077     853     608     538     450     328     307     162
                  71      58      18
 
AseI AT'TA_AT

Cuts at:      0     161    4470 
   Size:        161    4309
 
AvaI C'yCGr_G

Cuts at:      0     167    4470 
   Size:        167    4303
 
BamHI G'GATC_C

Cuts at:      0    3496    4470 
   Size:       3496     974
 
BanI G'GyrC_C

Cuts at:      0    4311    4344    4470 
   Size:       4311      33     126

  Fragments arranged by size: 

                4311     126      33
 
BanII G_rGCy'C

Cuts at:      0    2194    3153    3739    4116    4470 
   Size:       2194     959     586     377     354

  Fragments arranged by size: 

                2194     959     586     377     354
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    4027    4470 
   Size:       4027     443
 
BbvCI CC'TCA_GC

Cuts at:      0    4316    4470 
   Size:       4316     154
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3667    3701    3731    3765    4470 
   Size:       3667      34      30      34     705

  Fragments arranged by size: 

                3667     705      34      34      30
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     591    1761    4470 
   Size:        591    1170    2709

  Fragments arranged by size: 

                2709    1170     591
 
BclI T'GATC_A

Cuts at:      0    1440    2733    2883    4470 
   Size:       1440    1293     150    1587

  Fragments arranged by size: 

                1587    1440    1293     150
 
BfrBI ATG'CAT

Cuts at:      0    1655    4470 
   Size:       1655    2815
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3082    4470 
   Size:       3082    1388
 
Bme1580I G_kGCm'C

Cuts at:      0    4349    4470 
   Size:       4349     121
 
BmgBI CAC'GTC

Cuts at:      0    3180    3619    4277    4470 
   Size:       3180     439     658     193

  Fragments arranged by size: 

                3180     658     439     193
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3004    4470 
   Size:       3004    1466
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1481    1523    3046    3212    4470 
   Size:       1481      42    1523     166    1258

  Fragments arranged by size: 

                1523    1481    1258     166      42
 
Bpu10I CC'TnA_GC

Cuts at:      0    4316    4470 
   Size:       4316     154
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4372    4470 
   Size:       4372      98
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1431    4470 
   Size:       1431    3039
 
BsaBI GATnn'nnATC

Cuts at:      0    2882    4470 
   Size:       2882    1588
 
BsaWI w'CCGG_w

Cuts at:      0    4145    4470 
   Size:       4145     325
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2654    2684    3213    3243    4470 
   Size:       2654      30     529      30    1227

  Fragments arranged by size: 

                2654    1227     529      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     631    2302    2311    2534    4470 
   Size:        631    1671       9     223    1936

  Fragments arranged by size: 

                1936    1671     631     223       9
 
BseYI C'CCAG_C

Cuts at:      0    3738    4050    4284    4470 
   Size:       3738     312     234     186

  Fragments arranged by size: 

                3738     312     234     186
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2475    2837    4375    4470 
   Size:       2475     362    1538      95

  Fragments arranged by size: 

                2475    1538     362      95
 
BsiEI CG_ry'CG

Cuts at:      0     101    4470 
   Size:        101    4369
 
BsiHKAI G_wGCw'C

Cuts at:      0     174    4116    4457    4470 
   Size:        174    3942     341      13

  Fragments arranged by size: 

                3942     341     174      13
 
BsmI GAATG_Cn'

Cuts at:      0    2480    4470 
   Size:       2480    1990
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1893    4470 
   Size:       1893    2577
 
Bsp1286I G_dGCh'C

Cuts at:      0     174    2194    3153    3739    4116    4349    4457    4470 
   Size:        174    2020     959     586     377     233     108      13

  Fragments arranged by size: 

                2020     959     586     377     233     174     108      13
            
 
BspEI T'CCGG_A

Cuts at:      0    4145    4470 
   Size:       4145     325
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     864    1308    4052    4470 
   Size:        864     444    2744     418

  Fragments arranged by size: 

                2744     864     444     418
 
BsrBI CCG'CTC

Cuts at:      0    2869    3758    4089    4470 
   Size:       2869     889     331     381

  Fragments arranged by size: 

                2869     889     381     331
 
BsrDI GCAATG_nn'

Cuts at:      0      57    1121    3782    4470 
   Size:         57    1064    2661     688

  Fragments arranged by size: 

                2661    1064     688      57
 
BsrGI T'GTAC_A

Cuts at:      0     930    1238    4470 
   Size:        930     308    3232

  Fragments arranged by size: 

                3232     930     308
 
BssSI C'ACGA_G

Cuts at:      0    3899    3973    4470 
   Size:       3899      74     497

  Fragments arranged by size: 

                3899     497      74
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3879    4470 
   Size:       3879     591
 
BstYI r'GATC_y

Cuts at:      0     138    3496    3966    4012    4470 
   Size:        138    3358     470      46     458

  Fragments arranged by size: 

                3358     470     458     138      46
 
Bsu36I CC'TnA_GG

Cuts at:      0    3240    3470    4470 
   Size:       3240     230    1000

  Fragments arranged by size: 

                3240    1000     230
 
BtgI C'CryG_G

Cuts at:      0     393    1380    3224    4119    4170    4470 
   Size:        393     987    1844     895      51     300

  Fragments arranged by size: 

                1844     987     895     393     300      51
 
BtsI GCAGTG_nn'

Cuts at:      0     897    1590    2393    2487    4190    4202    4279    4470 
   Size:        897     693     803      94    1703      12      77     191

  Fragments arranged by size: 

                1703     897     803     693     191      94      77      12
            
 
ClaI AT'CG_AT

Cuts at:      0    3054    4470 
   Size:       3054    1416
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3942    3977    4470 
   Size:       3942      35     493

  Fragments arranged by size: 

                3942     493      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1381    4470 
   Size:       1381    3089
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     128    4470 
   Size:        128    4342
 
EaeI y'GGCC_r

Cuts at:      0    1313    1360    3512    4470 
   Size:       1313      47    2152     958

  Fragments arranged by size: 

                2152    1313     958      47
 
EarI CTCTTCn'nnn_

Cuts at:      0    1347    2230    2279    3263    4470 
   Size:       1347     883      49     984    1207

  Fragments arranged by size: 

                1347    1207     984     883      49
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3112    4470 
   Size:       3112    1358
 
EcoICRI GAG'CTC

Cuts at:      0    4114    4470 
   Size:       4114     356
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577    1481    1523    2132    3046    3212    3269    4470 
   Size:        577     904      42     609     914     166      57    1201

  Fragments arranged by size: 

                1201     914     904     609     577     166      57      42
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2788    4470 
   Size:       2788    1682
 
EcoO109I rG'GnC_Cy

Cuts at:      0     754    2749    3084    3244    4470 
   Size:        754    1995     335     160    1226

  Fragments arranged by size: 

                1995    1226     754     335     160
 
EcoRV GAT'ATC

Cuts at:      0     467    1457    4222    4470 
   Size:        467     990    2765     248

  Fragments arranged by size: 

                2765     990     467     248
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     400     677    4470 
   Size:        400     277    3793

  Fragments arranged by size: 

                3793     400     277
 
HaeII r_GCGC'y

Cuts at:      0      52    4470 
   Size:         52    4418
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     607     639     781     813    1890    1922    3432    3464 
   Size:        607      32     142      32    1077      32    1510      32

Cuts at:   3464    4470 
   Size:       1006

  Fragments arranged by size: 

                1510    1077    1006     607     142      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1878    3285    3485    4470 
   Size:       1878    1407     200     985

  Fragments arranged by size: 

                1878    1407     985     200
 
HindIII A'AGCT_T

Cuts at:      0     215    4470 
   Size:        215    4255
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2253    2833    2929    3524    4089    4439    4470 
   Size:       2253     580      96     595     565     350      31

  Fragments arranged by size: 

                2253     595     580     565     350      96      31
 
MscI TGG'CCA

Cuts at:      0    1362    3514    4470 
   Size:       1362    2152     956

  Fragments arranged by size: 

                2152    1362     956
 
MslI CAynn'nnrTG

Cuts at:      0    1535    2065    3649    3718    3994    4195    4297    4432 
   Size:       1535     530    1584      69     276     201     102     135

Cuts at:   4432    4470 
   Size:         38

  Fragments arranged by size: 

                1584    1535     530     276     201     135     102      69
                  38
 
MspA1I CmG'CkG

Cuts at:      0      70    1084    1188    1326    3658    3745    4320    4470 
   Size:         70    1014     104     138    2332      87     575     150

  Fragments arranged by size: 

                2332    1014     575     150     138     104      87      70
            
 
NcoI C'CATG_G

Cuts at:      0    4119    4170    4470 
   Size:       4119      51     300

  Fragments arranged by size: 

                4119     300      51
 
NdeI CA'TA_TG

Cuts at:      0    2528    2738    4470 
   Size:       2528     210    1732

  Fragments arranged by size: 

                2528    1732     210
 
NsiI A_TGCA'T

Cuts at:      0    1657    4470 
   Size:       1657    2813
 
NspI r_CATG'y

Cuts at:      0     594    1347    1406    2068    3772    4302    4470 
   Size:        594     753      59     662    1704     530     168

  Fragments arranged by size: 

                1704     753     662     594     530     168      59
 
PciI A'CATG_T

Cuts at:      0    1402    2064    4470 
   Size:       1402     662    2406

  Fragments arranged by size: 

                2406    1402     662
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     710    1252    4470 
   Size:        710     542    3218

  Fragments arranged by size: 

                3218     710     542
 
PfoI T'CCnGG_A

Cuts at:      0    3260    4470 
   Size:       3260    1210
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1331    1363    2204    2236    4470 
   Size:       1331      32     841      32    2234

  Fragments arranged by size: 

                2234    1331     841      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     754    2749    4470 
   Size:        754    1995    1721

  Fragments arranged by size: 

                1995    1721     754
 
PsiI TTA'TAA

Cuts at:      0    2552    4470 
   Size:       2552    1918
 
PspXI vC'TCGA_Gb

Cuts at:      0     167    4470 
   Size:        167    4303
 
PstI C_TGCA'G

Cuts at:      0    4063    4197    4470 
   Size:       4063     134     273

  Fragments arranged by size: 

                4063     273     134
 
PvuI CG_AT'CG

Cuts at:      0     101    4470 
   Size:        101    4369
 
PvuII CAG'CTG

Cuts at:      0    1084    1188    1326    3745    4320    4470 
   Size:       1084     104     138    2419     575     150

  Fragments arranged by size: 

                2419    1084     575     150     138     104
 
SacI G_AGCT'C

Cuts at:      0    4116    4470 
   Size:       4116     354
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3263    4470 
   Size:       3263    1207
 
ScaI AGT'ACT

Cuts at:      0    2444    4470 
   Size:       2444    2026
 
SexAI A'CCwGG_T

Cuts at:      0    1721    4340    4470 
   Size:       1721    2619     130

  Fragments arranged by size: 

                2619    1721     130
 
SfcI C'TryA_G

Cuts at:      0    1572    1604    3550    4059    4193    4470 
   Size:       1572      32    1946     509     134     277

  Fragments arranged by size: 

                1946    1572     509     277     134      32
 
SmlI C'TyrA_G

Cuts at:      0     167    4387    4470 
   Size:        167    4220      83

  Fragments arranged by size: 

                4220     167      83
 
SpeI A'CTAG_T

Cuts at:      0    3487    4470 
   Size:       3487     983
 
SphI G_CATG'C

Cuts at:      0    3772    4302    4470 
   Size:       3772     530     168

  Fragments arranged by size: 

                3772     530     168
 
SspI AAT'ATT

Cuts at:      0     611     709    1015    1062    2005    2823    4470 
   Size:        611      98     306      47     943     818    1647

  Fragments arranged by size: 

                1647     943     818     611     306      98      47
 
StuI AGG'CCT

Cuts at:      0    1707    3015    4470 
   Size:       1707    1308    1455

  Fragments arranged by size: 

                1707    1455    1308
 
StyI C'CwwG_G

Cuts at:      0    2469    4119    4170    4470 
   Size:       2469    1650      51     300

  Fragments arranged by size: 

                2469    1650     300      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2330    2861    4470 
   Size:       2330     531    1609

  Fragments arranged by size: 

                2330    1609     531
 
TatI w'GTAC_w

Cuts at:      0     930    1238    1320    1584    2442    3167    3998    4470 
   Size:        930     308      82     264     858     725     831     472

  Fragments arranged by size: 

                 930     858     831     725     472     308     264      82
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2654    2686    3213    3245    4470 
   Size:       2654      32     527      32    1225

  Fragments arranged by size: 

                2654    1225     527      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     793    1904    3178    4470 
   Size:        793    1111    1274    1292

  Fragments arranged by size: 

                1292    1274    1111     793
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     358    3507    3717    3960    4470 
   Size:        358    3149     210     243     510

  Fragments arranged by size: 

                3149     510     358     243     210
 
XhoI C'TCGA_G

Cuts at:      0     167    4470 
   Size:        167    4303
 
XmnI GAAnn'nnTTC

Cuts at:      0    1838    4470 
   Size:       1838    2632
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     AseI     AvaI
    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrGI    BssSI   BstAPI    BstYI   Bsu36I     BtgI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI    PspXI     PstI
     PvuI    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SmlI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
     BbeI    BglII     BlpI     BmtI     BplI    BsaAI    BsaHI    BsiWI
    BspHI    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     DraI     EagI    EcoRI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PmeI     PmlI    PshAI   PspOMI     PsrI
    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SrfI     SwaI     XbaI     XmaI     ZraI
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