Restriction map for pF1KA0476
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AflII       AflIII      AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BbeI        BclI        BfrI        BglII       BlnI        
BsaBI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FbaI        FseI        HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       PvuI        
RcaI        RsrII       SacII       SalI        SfoI        
SfuI        SnaBI       SpeI        SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI         1474  GTAGAC               GT^[AC][GT]AC
AccIII       2630  TCCGGA               T^CCGGA
AclI         1078  AACGTT               AA^CGTT
Alw44I       4213  GTGCAC               G^TGCAC
ApaLI        4213  GTGCAC               G^TGCAC
AviII         935  TGCGCA               TGC^GCA
BbrPI        4333  CACGTG               CAC^GTG
BbvCI        4410  GCTGAGG              CCTCAGC(-5/-2)
BsaAI        4333  CACGTG               [CT]AC^GT[GA]
BsaWI        2630  TCCGGA               [AT]^CCGG[AT]
BseAI        2630  TCCGGA               T^CCGGA
BsiEI        2350  CGGTCG               CG[GA][CT]^CG
BspEI        2630  TCCGGA               T^CCGGA
BssSI        1787  CTCGTG               CACGAG(-5/-1)
DraIII       2600  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        1939  GAATTC               G^AATTC
EcoT22I       198  ATGCAT               ATGCA^T
FspI          935  TGCGCA               TGC^GCA
HincII       1795  GTTGAC               GT[CT]^[GA]AC
HindII       1795  GTTGAC               GT[CT]^[GA]AC
MroI         2630  TCCGGA               T^CCGGA
MseI         4376  TTAA                 T^TAA
NsiI          198  ATGCAT               ATGCA^T
PmaCI        4333  CACGTG               CAC^GTG
PmlI         4333  CACGTG               CAC^GTG
Psp1406I     1078  AACGTT               AA^CGTT
SapI         1494  GCTCTTC              GCTCTTC(1/4)
SbfI         1812  CCTGCAGG             CCTGCA^GG
ScaI         2099  AGTACT               AGT^ACT
SexAI        2601  ACCAGGT              A^CC[AT]GGT
SfiI         2925  GGCCGGTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        4417  CACCGGCG             C[GA]^CCGG[CT]G
SphI          468  GCATGC               GCATG^C
Sse8387I     1812  CCTGCAGG             CCTGCA^GG
Tru9I        4376  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         991  CGCG                 CG^CG
             1690  CGCG                
AcsI         1939  GAATTC               [GA]^AATT[CT]
             3452  AAATTC              
AcyI         2609  GGCGTC               G[GA]^CG[CT]C
             2867  GACGCC              
AocI           76  CCTGAGG              CC^T[ACGT]AGG
             1615  CCTGAGG             
ApoI         1939  GAATTC               [GA]^AATT[CT]
             3452  AAATTC              
BamHI        3313  GGATCC               G^GATCC
             3427  GGATCC              
BcgI         2058  CGAGGAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3706  CGAAGGCTCTGC        
BciVI        1159  GTATCC               GTATCC(6/5)
             1421  GTATCC              
BsaHI        2609  GGCGTC               G[GA]^CG[CT]C
             2867  GACGCC              
BsaI         1101  GAGACC               GGTCTC(1/5)
             2846  GAGACC              
BsmBI        1023  CGTCTC               CGTCTC(1/5)
             1320  CGTCTC              
BsmI         4483  GAATGC               GAATGC(1/-1)
             3224  GCATTC              
BstUI         991  CGCG                 CG^CG
             1690  CGCG                
Bsu36I         76  CCTGAGG              CC^T[ACGT]AGG
             1615  CCTGAGG             
Eco81I         76  CCTGAGG              CC^T[ACGT]AGG
             1615  CCTGAGG             
EcoNI         606  CCTGGCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2173  CCTGTGCCAGG         
Hin1I        2609  GGCGTC               G[GA]^CG[CT]C
             2867  GACGCC              
MvnI          991  CGCG                 CG^CG
             1690  CGCG                
NaeI          539  GCCGGC               GCC^GGC
              941  GCCGGC              
NcoI          716  CCATGG               C^CATGG
             3278  CCATGG              
NgoMIV        539  GCCGGC               G^CCGGC
              941  GCCGGC              
SmlI         1732  CTCAAG               C^T[CT][GA]AG
             2670  CTTGAG              
StuI         2948  AGGCCT               AGG^CCT
             3954  AGGCCT              
Tsp509I      1940  AATT                 ^AATT
             3453  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI        
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