Restriction map for pF1KA0474
 (Linear) (Six-base) MAPSORT of: KIAA0474.seq  Check: 5471  from: 1  to: 2043



KIAA0474.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     386    2043 
   Size:        386    1657
 
AatII G_ACGT'C

Cuts at:      0     491    2043 
   Size:        491    1552
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615    2043 
   Size:        615    1428
 
AflIII A'CryG_T

Cuts at:      0     800    2043 
   Size:        800    1243
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1190    1222    2043 
   Size:       1190      32     821

  Fragments arranged by size: 

                1190     821      32
 
ApaI G_GGCC'C

Cuts at:      0     979    1552    2043 
   Size:        979     573     491

  Fragments arranged by size: 

                 979     573     491
 
ApaLI G'TGCA_C

Cuts at:      0      97    2043 
   Size:         97    1946
 
ApoI r'AATT_y

Cuts at:      0     673    1087    2043 
   Size:        673     414     956

  Fragments arranged by size: 

                 956     673     414
 
AvaI C'yCGr_G

Cuts at:      0      90     199     241    1987    2043 
   Size:         90     109      42    1746      56

  Fragments arranged by size: 

                1746     109      90      56      42
 
BanI G'GyrC_C

Cuts at:      0     172    2043 
   Size:        172    1871
 
BanII G_rGCy'C

Cuts at:      0     979    1531    1552    1624    1673    1702    2043 
   Size:        979     552      21      72      49      29     341

  Fragments arranged by size: 

                 979     552     341      72      49      29      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     330     491     879    1645    2043 
   Size:        330     161     388     766     398

  Fragments arranged by size: 

                 766     398     388     330     161
 
BbvCI CC'TCA_GC

Cuts at:      0     965    2043 
   Size:        965    1078
 
BclI T'GATC_A

Cuts at:      0    1106    2043 
   Size:       1106     937
 
BglI GCCn_nnn'nGGC

Cuts at:      0     983    1417    1556    2043 
   Size:        983     434     139     487

  Fragments arranged by size: 

                 983     487     434     139
 
BlpI GC'TnA_GC

Cuts at:      0    1339    2043 
   Size:       1339     704
 
Bme1580I G_kGCm'C

Cuts at:      0     101     175     917     979    1028    1552    1658    2043 
   Size:        101      74     742      62      49     524     106     385

  Fragments arranged by size: 

                 742     524     385     106     101      74      62      49
            
 
BmgBI CAC'GTC

Cuts at:      0     732    1659    2043 
   Size:        732     927     384

  Fragments arranged by size: 

                 927     732     384
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     141    2043 
   Size:        141    1902
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1174    1201    1873    1936    2043 
   Size:       1174      27     672      63     107

  Fragments arranged by size: 

                1174     672     107      63      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     391     965    2043 
   Size:        391     574    1078

  Fragments arranged by size: 

                1078     574     391
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1747    2043 
   Size:        331    1416     296

  Fragments arranged by size: 

                1416     331     296
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1005    1729    2043 
   Size:       1005     724     314

  Fragments arranged by size: 

                1005     724     314
 
BsaAI yAC'GTr

Cuts at:      0     801    1910    2043 
   Size:        801    1109     133

  Fragments arranged by size: 

                1109     801     133
 
BsaBI GATnn'nnATC

Cuts at:      0    1996    2043 
   Size:       1996      47
 
BsaHI Gr'CG_yC

Cuts at:      0     488     587     833    1467    1958    2043 
   Size:        488      99     246     634     491      85

  Fragments arranged by size: 

                 634     491     488     246      99      85
 
BsaWI w'CCGG_w

Cuts at:      0     269    1304    2043 
   Size:        269    1035     739

  Fragments arranged by size: 

                1035     739     269
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     778     808    1028    1058    1660    1690    1846    1876 
   Size:        778      30     220      30     602      30     156      30

Cuts at:   1876    2043 
   Size:        167

  Fragments arranged by size: 

                 778     602     220     167     156      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     734    1171    1770    1831    2043 
   Size:         85     649     437     599      61     212

  Fragments arranged by size: 

                 649     599     437     212      85      61
 
BseYI C'CCAG_C

Cuts at:      0     836    2030    2034    2043 
   Size:        836    1194       4       9

  Fragments arranged by size: 

                1194     836       9       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     979    1624    2043 
   Size:        979     645     419

  Fragments arranged by size: 

                 979     645     419
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     377     518    1702    2043 
   Size:        101     276     141    1184     341

  Fragments arranged by size: 

                1184     341     276     141     101
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1177    1665    1813    2043 
   Size:       1177     488     148     230

  Fragments arranged by size: 

                1177     488     230     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     175     377     518     917     979    1028    1531 
   Size:        101      74     202     141     399      62      49     503

Cuts at:   1531    1552    1624    1658    1673    1702    2043 
   Size:         21      72      34      15      29     341

  Fragments arranged by size: 

                 503     399     341     202     141     101      74      72
                  62      49      34      29      21      15
 
BspEI T'CCGG_A

Cuts at:      0    1304    2043 
   Size:       1304     739
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     386    2043 
   Size:        386    1657
 
BsrBI CCG'CTC

Cuts at:      0    1161    1732    1778    2043 
   Size:       1161     571      46     265

  Fragments arranged by size: 

                1161     571     265      46
 
BsrDI GCAATG_nn'

Cuts at:      0    1429    2043 
   Size:       1429     614
 
BsrFI r'CCGG_y

Cuts at:      0    1960    2043 
   Size:       1960      83
 
BssSI C'ACGA_G

Cuts at:      0     100    1533    2043 
   Size:        100    1433     510

  Fragments arranged by size: 

                1433     510     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2025    2043 
   Size:       2025      18
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1812    2043 
   Size:       1812     231
 
BstYI r'GATC_y

Cuts at:      0    1997    2043 
   Size:       1997      46
 
Bsu36I CC'TnA_GG

Cuts at:      0    1869    2043 
   Size:       1869     174
 
BtgI C'CryG_G

Cuts at:      0     738    1322    1331    1496    2043 
   Size:        738     584       9     165     547

  Fragments arranged by size: 

                 738     584     547     165       9
 
BtsI GCAGTG_nn'

Cuts at:      0     211    1007    1269    1482    2043 
   Size:        211     796     262     213     561

  Fragments arranged by size: 

                 796     561     262     213     211
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     559    2043 
   Size:        559    1484
 
EaeI y'GGCC_r

Cuts at:      0     327     681    1409    2043 
   Size:        327     354     728     634

  Fragments arranged by size: 

                 728     634     354     327
 
EarI CTCTTCn'nnn_

Cuts at:      0     634    1496    1519    1606    2043 
   Size:        634     862      23      87     437

  Fragments arranged by size: 

                 862     634     437      87      23
 
EcoICRI GAG'CTC

Cuts at:      0    1700    2043 
   Size:       1700     343
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615    1174    1201    1873    1936    2043 
   Size:        615     559      27     672      63     107

  Fragments arranged by size: 

                 672     615     559     107      63      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     994    1411    2043 
   Size:        994     417     632

  Fragments arranged by size: 

                 994     632     417
 
EcoO109I rG'GnC_Cy

Cuts at:      0     121     975     976    1072    1974    2043 
   Size:        121     854       1      96     902      69

  Fragments arranged by size: 

                 902     854     121      96      69       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     671    2043 
   Size:        671    1372
 
HaeII r_GCGC'y

Cuts at:      0    1577    1762    2043 
   Size:       1577     185     281

  Fragments arranged by size: 

                1577     281     185
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1028    1060    1660    1672    1692    1704    1846    1878 
   Size:       1028      32     600      12      20      12     142      32

Cuts at:   1878    2043 
   Size:        165

  Fragments arranged by size: 

                1028     600     165     142      32      32      20      12
                  12
 
HindIII A'AGCT_T

Cuts at:      0     601    2043 
   Size:        601    1442
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     956    1658    2043 
   Size:        956     702     385

  Fragments arranged by size: 

                 956     702     385
 
MscI TGG'CCA

Cuts at:      0     683    1411    2043 
   Size:        683     728     632

  Fragments arranged by size: 

                 728     683     632
 
MspA1I CmG'CkG

Cuts at:      0     849    1057    1379    1652    2004    2034    2043 
   Size:        849     208     322     273     352      30       9

  Fragments arranged by size: 

                 849     352     322     273     208      30       9
 
NaeI GCC'GGC

Cuts at:      0    1962    2043 
   Size:       1962      81
 
NcoI C'CATG_G

Cuts at:      0    1322    1331    2043 
   Size:       1322       9     712

  Fragments arranged by size: 

                1322     712       9
 
NgoMIV G'CCGG_C

Cuts at:      0    1960    2043 
   Size:       1960      83
 
NspI r_CATG'y

Cuts at:      0     562     946    2028    2043 
   Size:        562     384    1082      15

  Fragments arranged by size: 

                1082     562     384      15
 
PasI CC'CwG_GG

Cuts at:      0    1519    2043 
   Size:       1519     524
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1323    2043 
   Size:       1323     720
 
PfoI T'CCnGG_A

Cuts at:      0     890    1046    1082    1178    2043 
   Size:        890     156      36      96     865

  Fragments arranged by size: 

                 890     865     156      96      36
 
PmlI CAC'GTG

Cuts at:      0     801    1910    2043 
   Size:        801    1109     133

  Fragments arranged by size: 

                1109     801     133
 
PpuMI rG'GwC_Cy

Cuts at:      0     121    1974    2043 
   Size:        121    1853      69

  Fragments arranged by size: 

                1853     121      69
 
PspOMI G'GGCC_C

Cuts at:      0     975    1548    2043 
   Size:        975     573     495

  Fragments arranged by size: 

                 975     573     495
 
PspXI vC'TCGA_Gb

Cuts at:      0     241    2043 
   Size:        241    1802
 
PstI C_TGCA'G

Cuts at:      0     218     701    2043 
   Size:        218     483    1342

  Fragments arranged by size: 

                1342     483     218
 
PvuII CAG'CTG

Cuts at:      0    2004    2034    2043 
   Size:       2004      30       9

  Fragments arranged by size: 

                2004      30       9
 
SacI G_AGCT'C

Cuts at:      0    1702    2043 
   Size:       1702     341
 
SanDI GG'GwC_CC

Cuts at:      0    1974    2043 
   Size:       1974      69
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1519    2043 
   Size:       1519     524
 
SfcI C'TryA_G

Cuts at:      0     214     697    1902    2043 
   Size:        214     483    1205     141

  Fragments arranged by size: 

                1205     483     214     141
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     983    2043 
   Size:        983    1060
 
SmlI C'TyrA_G

Cuts at:      0     241     346    1762    2043 
   Size:        241     105    1416     281

  Fragments arranged by size: 

                1416     281     241     105
 
SpeI A'CTAG_T

Cuts at:      0    1936    2043 
   Size:       1936     107
 
SphI G_CATG'C

Cuts at:      0     946    2043 
   Size:        946    1097
 
StuI AGG'CCT

Cuts at:      0     725    1850    2043 
   Size:        725    1125     193

  Fragments arranged by size: 

                1125     725     193
 
StyI C'CwwG_G

Cuts at:      0     230     524    1322    1331    1412    2043 
   Size:        230     294     798       9      81     631

  Fragments arranged by size: 

                 798     631     294     230      81       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     769    2043 
   Size:        769    1274
 
TatI w'GTAC_w

Cuts at:      0     764    2043 
   Size:        764    1279
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1201    1233    1499    1531    1966    1998    2043 
   Size:       1201      32     266      32     435      32      45

  Fragments arranged by size: 

                1201     435     266      45      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1924    2043 
   Size:       1924     119
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1227    1329    2043 
   Size:       1227     102     714

  Fragments arranged by size: 

                1227     714     102
 
XhoI C'TCGA_G

Cuts at:      0     241    2043 
   Size:        241    1802
 
ZraI GAC'GTC

Cuts at:      0     489    2043 
   Size:        489    1554
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AloI     ApaI    ApaLI     ApoI
     AvaI     BanI    BanII     BbsI    BbvCI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
  HindIII     MmeI     MscI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SacI    SanDI     SapI     SfcI     SfiI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI    AlwNI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BcgI    BciVI    BfrBI    BglII     BmtI     BplI    BsiEI
    BsiWI     BsmI    BspHI    BsrGI   BssHII    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MslI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI     PsrI
     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI     SwaI     XbaI
     XmaI     XmnI
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