Restriction map for pF1KA0472
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrGI       BssHII      
BstAPI      BstBI       BstEII      BstPI       CelII       
ClaI        CpoI        Eam1105I    Eco47III    EcoO65I     
EcoT22I     FseI        FspI        HgaI        HincII      
HindII      HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NaeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
ScaI        SfuI        SgrAI       SmaI        SnaBI       
SphI        SspBI       SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        1324  GACGTC               GACGT^C
AccIII       1766  TCCGGA               T^CCGGA
AflIII       2257  ACACGT               A^C[GA][CT]GT
BbeI          452  GGCGCC               GGCGC^C
BbsI         2618  GTCTTC               GAAGAC(2/6)
BcgI         1791  CGAGGCTTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         975  GGATAC               GTATCC(6/5)
BclI         1439  TGATCA               T^GATCA
BpuAI        2618  GTCTTC               GAAGAC(2/6)
BsaWI        1766  TCCGGA               [AT]^CCGG[AT]
BseAI        1766  TCCGGA               T^CCGGA
BsiEI          93  CGGCCG               CG[GA][CT]^CG
BsmBI          36  CGTCTC               CGTCTC(1/5)
BspEI        1766  TCCGGA               T^CCGGA
BspHI        2726  TCATGA               T^CATGA
BsrBI        2203  GAGCGG               CCGCTC(-3/-3)
BsrDI        2181  CATTGC               GCAATG(2/0)
BsrFI        1838  GCCGGT               [GA]^CCGG[CT]
BssSI        1789  CACGAG               CACGAG(-5/-1)
Bst1107I     1996  GTATAC               GTA^TAC
BstZ17I      1996  GTATAC               GTA^TAC
Cfr10I       1838  GCCGGT               [GA]^CCGG[CT]
DraI         1318  TTTAAA               TTT^AAA
DraIII       1042  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1324  GACGTCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           93  CGGCCG               C^GGCCG
EclXI          93  CGGCCG               C^GGCCG
Eco52I         93  CGGCCG               C^GGCCG
EcoRI        2504  GAATTC               G^AATTC
EcoRV        2329  GATATC               GAT^ATC
FbaI         1439  TGATCA               T^GATCA
KasI          452  GGCGCC               G^GCGCC
MroI         1766  TCCGGA               T^CCGGA
NarI          452  GGCGCC               GG^CGCC
NdeI         2443  CATATG               CA^TATG
NheI         2566  GCTAGC               G^CTAGC
PshAI         480  GACTCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2726  TCATGA               T^CATGA
SbfI          229  CCTGCAGG             CCTGCA^GG
SfiI         1828  GGCCACTCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          452  GGCGCC               GGC^GCC
SmlI         1026  CTTGAG               C^T[CT][GA]AG
SpeI          516  ACTAGT               A^CTAGT
Sse8387I      229  CCTGCAGG             CCTGCA^GG
SspI         1219  AATATT               AAT^ATT
TaqI         1182  TCGA                 T^CGA
TthHB8I      1182  TCGA                 T^CGA
XcmI          413  CCACCACCAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1996  GTATAC               GT^[AC][GT]AC
             2491  GTCTAC              
AcsI         2089  GAATTT               [GA]^AATT[CT]
             2504  GAATTC              
AcyI          452  GGCGCC               G[GA]^CG[CT]C
             1324  GACGTC              
AfaI          306  GTAC                 GT^AC
             2472  GTAC                
Alw44I        520  GTGCAC               G^TGCAC
             1093  GTGCAC              
ApaLI         520  GTGCAC               G^TGCAC
             1093  GTGCAC              
ApoI         2089  GAATTT               [GA]^AATT[CT]
             2504  GAATTC              
AvaI           39  CTCGGG               C^[CT]CG[GA]G
              482  CTCGGG              
BalI          969  TGGCCA               TGG^CCA
             1827  TGGCCA              
BbvCI        1725  CCTCAGC              CCTCAGC(-5/-2)
             1866  GCTGAGG             
BglII        1556  AGATCT               A^GATCT
             1857  AGATCT              
BsaHI         452  GGCGCC               G[GA]^CG[CT]C
             1324  GACGTC              
BsgI         2302  CTGCAC               GTGCAG(16/14)
              400  GTGCAG              
BsoBI          39  CTCGGG               C^[CT]CG[GA]G
              482  CTCGGG              
Hin1I         452  GGCGCC               G[GA]^CG[CT]C
             1324  GACGTC              
HindIII       445  AAGCTT               A^AGCTT
             1467  AAGCTT              
MluNI         969  TGGCCA               TGG^CCA
             1827  TGGCCA              
MscI          969  TGGCCA               TGG^CCA
             1827  TGGCCA              
NcoI           19  CCATGG               C^CATGG
             2139  CCATGG              
NspI          992  GCATGT               [GA]CATG^[CT]
             1136  ACATGC              
PpuMI          42  GGGTCCC              [GA]G^G[AT]CC[CT]
             1145  GGGACCT             
PvuII         376  CAGCTG               CAG^CTG
             1816  CAGCTG              
RsaI          306  GTAC                 GT^AC
             2472  GTAC                
SexAI        1043  ACCAGGT              A^CC[AT]GGT
             1088  ACCTGGT             
StuI          684  AGGCCT               AGG^CCT
             2686  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AocI        Asp700I     AspHI       AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
XmnI        
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