Restriction map for pF1KA0472
 (Linear) (Six-base) MAPSORT of: KIAA0472.seq  Check: 7554  from: 1  to: 2787



KIAA0472.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     393    1680    2787 
   Size:        393    1287    1107

  Fragments arranged by size: 

                1287    1107     393
 
AatII G_ACGT'C

Cuts at:      0    1328    2787 
   Size:       1328    1459
 
AccI GT'mk_AC

Cuts at:      0    1997    2492    2787 
   Size:       1997     495     295

  Fragments arranged by size: 

                1997     495     295
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     778    2254    2787 
   Size:        778    1476     533

  Fragments arranged by size: 

                1476     778     533
 
AflIII A'CryG_T

Cuts at:      0    2257    2787 
   Size:       2257     530
 
AleI CACnn'nnGTG

Cuts at:      0    1660    2787 
   Size:       1660    1127
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2203    2384    2787 
   Size:       2203     181     403

  Fragments arranged by size: 

                2203     403     181
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     950    1503    1971    2533    2787 
   Size:        132     818     553     468     562     254

  Fragments arranged by size: 

                 818     562     553     468     254     132
 
ApaLI G'TGCA_C

Cuts at:      0     520    1093    2787 
   Size:        520     573    1694

  Fragments arranged by size: 

                1694     573     520
 
ApoI r'AATT_y

Cuts at:      0    2089    2504    2787 
   Size:       2089     415     283

  Fragments arranged by size: 

                2089     415     283
 
AvaI C'yCGr_G

Cuts at:      0      39     482    2787 
   Size:         39     443    2305

  Fragments arranged by size: 

                2305     443      39
 
BanI G'GyrC_C

Cuts at:      0      15     452    1357    2787 
   Size:         15     437     905    1430

  Fragments arranged by size: 

                1430     905     437      15
 
BanII G_rGCy'C

Cuts at:      0      35     856     966    1669    2607    2787 
   Size:         35     821     110     703     938     180

  Fragments arranged by size: 

                 938     821     703     180     110      35
 
BbeI G_GCGC'C

Cuts at:      0     456    2787 
   Size:        456    2331
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2611    2787 
   Size:       2611     176
 
BbvCI CC'TCA_GC

Cuts at:      0    1726    1867    2787 
   Size:       1726     141     920

  Fragments arranged by size: 

                1726     920     141
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1780    1814    2787 
   Size:       1780      34     973

  Fragments arranged by size: 

                1780     973      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     969    2787 
   Size:        969    1818
 
BclI T'GATC_A

Cuts at:      0    1439    2787 
   Size:       1439    1348
 
BglI GCCn_nnn'nGGC

Cuts at:      0      56     233    1835    2787 
   Size:         56     177    1602     952

  Fragments arranged by size: 

                1602     952     177      56
 
BglII A'GATC_T

Cuts at:      0    1556    1857    2787 
   Size:       1556     301     930

  Fragments arranged by size: 

                1556     930     301
 
Bme1580I G_kGCm'C

Cuts at:      0     524    1097    2037    2787 
   Size:        524     573     940     750

  Fragments arranged by size: 

                 940     750     573     524
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     558     859    1969    1981    2022    2787 
   Size:        558     301    1110      12      41     765

  Fragments arranged by size: 

                1110     765     558     301      41      12
 
BmtI G_CTAG'C

Cuts at:      0    2570    2787 
   Size:       2570     217
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      54    2621    2787 
   Size:         54    2567     166

  Fragments arranged by size: 

                2567     166      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     595     643    1525    2039    2160    2272    2701 
   Size:        295     300      48     882     514     121     112     429

Cuts at:   2701    2787 
   Size:         86

  Fragments arranged by size: 

                 882     514     429     300     295     121     112      86
                  48
 
Bpu10I CC'TnA_GC

Cuts at:      0    1726    1867    2526    2787 
   Size:       1726     141     659     261

  Fragments arranged by size: 

                1726     659     261     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1047    2787 
   Size:       1047    1740
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     133     547    2111    2787 
   Size:        133     414    1564     676

  Fragments arranged by size: 

                1564     676     414     133
 
BsaHI Gr'CG_yC

Cuts at:      0     453    1325    2787 
   Size:        453     872    1462

  Fragments arranged by size: 

                1462     872     453
 
BsaWI w'CCGG_w

Cuts at:      0    1766    2787 
   Size:       1766    1021
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1183    1213    1337    1367    2787 
   Size:       1183      30     124      30    1420

  Fragments arranged by size: 

                1420    1183     124      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     183     448     580     861    2182    2650    2787 
   Size:        183     265     132     281    1321     468     137

  Fragments arranged by size: 

                1321     468     281     265     183     137     132
 
BseYI C'CCAG_C

Cuts at:      0     423    2239    2787 
   Size:        423    1816     548

  Fragments arranged by size: 

                1816     548     423
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421    2287    2787 
   Size:        421    1866     500

  Fragments arranged by size: 

                1866     500     421
 
BsiEI CG_ry'CG

Cuts at:      0      96    2787 
   Size:         96    2691
 
BsiHKAI G_wGCw'C

Cuts at:      0     172     524    1033    1097    2192    2787 
   Size:        172     352     509      64    1095     595

  Fragments arranged by size: 

                1095     595     509     352     172      64
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      42    2787 
   Size:         42    2745
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     172     524     856     966    1033    1097    1669 
   Size:         35     137     352     332     110      67      64     572

Cuts at:   1669    2037    2192    2607    2787 
   Size:        368     155     415     180

  Fragments arranged by size: 

                 572     415     368     352     332     180     155     137
                 110      67      64      35
 
BspEI T'CCGG_A

Cuts at:      0    1766    2787 
   Size:       1766    1021
 
BspHI T'CATG_A

Cuts at:      0    2726    2787 
   Size:       2726      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     140     393    1157    1680    2787 
   Size:        140     253     764     523    1107

  Fragments arranged by size: 

                1107     764     523     253     140
 
BsrBI CCG'CTC

Cuts at:      0    2205    2787 
   Size:       2205     582
 
BsrDI GCAATG_nn'

Cuts at:      0    2180    2787 
   Size:       2180     607
 
BsrFI r'CCGG_y

Cuts at:      0    1838    2787 
   Size:       1838     949
 
BssSI C'ACGA_G

Cuts at:      0    1789    2787 
   Size:       1789     998
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     423     885    1170    1683    2787 
   Size:        423     462     285     513    1104

  Fragments arranged by size: 

                1104     513     462     423     285
 
BstYI r'GATC_y

Cuts at:      0     570    1388    1556    1857    2133    2217    2787 
   Size:        570     818     168     301     276      84     570

  Fragments arranged by size: 

                 818     570     570     301     276     168      84
 
BstZ17I GTA'TAC

Cuts at:      0    1998    2787 
   Size:       1998     789
 
Bsu36I CC'TnA_GG

Cuts at:      0     293     563    1459    2546    2787 
   Size:        293     270     896    1087     241

  Fragments arranged by size: 

                1087     896     293     270     241
 
BtgI C'CryG_G

Cuts at:      0      19    2139    2483    2787 
   Size:         19    2120     344     304

  Fragments arranged by size: 

                2120     344     304      19
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     251    2787 
   Size:        251    2536
 
BtsI GCAGTG_nn'

Cuts at:      0     435    1095    1101    1430    2787 
   Size:        435     660       6     329    1357

  Fragments arranged by size: 

                1357     660     435     329       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2148    2183    2787 
   Size:       2148      35     604

  Fragments arranged by size: 

                2148     604      35
 
DraI TTT'AAA

Cuts at:      0    1320    2787 
   Size:       1320    1467
 
DraIII CAC_nnn'GTG

Cuts at:      0    1047    2787 
   Size:       1047    1740
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1330    2787 
   Size:       1330    1457
 
EaeI y'GGCC_r

Cuts at:      0      93     351     969    1827    2787 
   Size:         93     258     618     858     960

  Fragments arranged by size: 

                 960     858     618     258      93
 
EagI C'GGCC_G

Cuts at:      0      93    2787 
   Size:         93    2694
 
EarI CTCTTCn'nnn_

Cuts at:      0     293    1687    2677    2787 
   Size:        293    1394     990     110

  Fragments arranged by size: 

                1394     990     293     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      77    2787 
   Size:         77    2710
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     595     643     778    1525    2039    2160    2254 
   Size:        295     300      48     135     747     514     121      94

Cuts at:   2254    2272    2701    2787 
   Size:         18     429      86

  Fragments arranged by size: 

                 747     514     429     300     295     135     121      94
                  86      48      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     352     876    2682    2787 
   Size:        352     524    1806     105

  Fragments arranged by size: 

                1806     524     352     105
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43    1080    1146    2787 
   Size:         43    1037      66    1641

  Fragments arranged by size: 

                1641    1037      66      43
 
EcoRI G'AATT_C

Cuts at:      0    2504    2787 
   Size:       2504     283
 
EcoRV GAT'ATC

Cuts at:      0    2331    2787 
   Size:       2331     456
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1940    2787 
   Size:       1940     847
 
HaeII r_GCGC'y

Cuts at:      0     456     499    1733    2787 
   Size:        456      43    1234    1054

  Fragments arranged by size: 

                1234    1054     456      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     465     497     754     786     930     962    2787 
   Size:        465      32     257      32     144      32    1825

  Fragments arranged by size: 

                1825     465     257     144      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     445    1467    2787 
   Size:        445    1022    1320

  Fragments arranged by size: 

                1320    1022     445
 
KasI G'GCGC_C

Cuts at:      0     452    2787 
   Size:        452    2335
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1407    1455    2787 
   Size:       1407      48    1332

  Fragments arranged by size: 

                1407    1332      48
 
MscI TGG'CCA

Cuts at:      0     971    1829    2787 
   Size:        971     858     958

  Fragments arranged by size: 

                 971     958     858
 
MslI CAynn'nnrTG

Cuts at:      0     761     997    1660    2072    2185    2787 
   Size:        761     236     663     412     113     602

  Fragments arranged by size: 

                 761     663     602     412     236     113
 
MspA1I CmG'CkG

Cuts at:      0     378    1143    1818    2787 
   Size:        378     765     675     969

  Fragments arranged by size: 

                 969     765     675     378
 
NarI GG'CG_CC

Cuts at:      0     453    2787 
   Size:        453    2334
 
NcoI C'CATG_G

Cuts at:      0      19    2139    2787 
   Size:         19    2120     648

  Fragments arranged by size: 

                2120     648      19
 
NdeI CA'TA_TG

Cuts at:      0    2444    2787 
   Size:       2444     343
 
NheI G'CTAG_C

Cuts at:      0    2566    2787 
   Size:       2566     221
 
NspI r_CATG'y

Cuts at:      0     996    1140    2787 
   Size:        996     144    1647

  Fragments arranged by size: 

                1647     996     144
 
PfoI T'CCnGG_A

Cuts at:      0    1391    2787 
   Size:       1391    1396
 
PpuMI rG'GwC_Cy

Cuts at:      0      43    1146    2787 
   Size:         43    1103    1641

  Fragments arranged by size: 

                1641    1103      43
 
PshAI GACnn'nnGTC

Cuts at:      0     484    2787 
   Size:        484    2303
 
PstI C_TGCA'G

Cuts at:      0     234     260    1154    1499    2787 
   Size:        234      26     894     345    1288

  Fragments arranged by size: 

                1288     894     345     234      26
 
PvuII CAG'CTG

Cuts at:      0     378    1818    2787 
   Size:        378    1440     969

  Fragments arranged by size: 

                1440     969     378
 
SanDI GG'GwC_CC

Cuts at:      0      43    2787 
   Size:         43    2744
 
SbfI CC_TGCA'GG

Cuts at:      0     234    2787 
   Size:        234    2553
 
SexAI A'CCwGG_T

Cuts at:      0    1043    1088    2787 
   Size:       1043      45    1699

  Fragments arranged by size: 

                1699    1043      45
 
SfcI C'TryA_G

Cuts at:      0     230     256    1150    1495    2787 
   Size:        230      26     894     345    1292

  Fragments arranged by size: 

                1292     894     345     230      26
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1835    2787 
   Size:       1835     952
 
SfoI GGC'GCC

Cuts at:      0     454    2787 
   Size:        454    2333
 
SmlI C'TyrA_G

Cuts at:      0    1026    2787 
   Size:       1026    1761
 
SpeI A'CTAG_T

Cuts at:      0     516    2787 
   Size:        516    2271
 
SspI AAT'ATT

Cuts at:      0    1221    2787 
   Size:       1221    1566
 
StuI AGG'CCT

Cuts at:      0     686    2688    2787 
   Size:        686    2002      99

  Fragments arranged by size: 

                2002     686      99
 
StyI C'CwwG_G

Cuts at:      0      19     681    1076    2139    2787 
   Size:         19     662     395    1063     648

  Fragments arranged by size: 

                1063     662     648     395      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     700    1650    2787 
   Size:        700     950    1137

  Fragments arranged by size: 

                1137     950     700
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     420    2787 
   Size:        420    2367
 
XmnI GAAnn'nnTTC

Cuts at:      0     151     727    2618    2787 
   Size:        151     576    1891     169

  Fragments arranged by size: 

                1891     576     169     151
 
ZraI GAC'GTC

Cuts at:      0    1326    2787 
   Size:       1326    1461
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     KasI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NspI
     PfoI    PpuMI    PshAI     PstI    PvuII    SanDI     SbfI    SexAI
     SfcI     SfiI     SfoI     SmlI     SpeI     SspI     StuI     StyI
    TaqII     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BfrBI     BlpI
    BmgBI    BsaAI    BsaBI    BsiWI     BsmI    BsrGI   BssHII   BstAPI
    BstBI   BstEII     ClaI  EcoICRI     FseI     FspI    FspAI   HincII
     HpaI     KpnI     MfeI     MluI     NaeI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SapI     ScaI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com