Restriction map for pF1KA0459
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssSI       Bst1107I    BstBI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
Eam1105I    Eco47III    Eco81I      EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MflI        MluI        MroI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII          86  CGCG                 CG^CG
AlwI          563  GGATC                GGATC(4/5)
ApaI           92  GGGCCC               GGGCC^C
AscI           84  GGCGCGCC             GG^CGCGCC
Asp700I      1170  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         950  GGACC                G^G[AT]CC
BclI          246  TGATCA               T^GATCA
BfaI          980  CTAG                 C^TAG
BsgI          587  CTGCAC               GTGCAG(16/14)
BsmAI         292  GAGAC                GTCTC(1/5)
BsrDI         404  GCAATG               GCAATG(2/0)
BsrFI          26  GCCGGC               [GA]^CCGG[CT]
BssHII         85  GCGCGC               G^CGCGC
BstAPI        793  GCAATAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1158  GGTCACC              G^GT[ACGT]ACC
BstPI        1158  GGTCACC              G^GT[ACGT]ACC
BstUI          86  CGCG                 CG^CG
Cfr10I         26  GCCGGC               [GA]^CCGG[CT]
EcoNI          96  CCTGGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1158  GGTCACC              G^GT[ACGT]ACC
FbaI          246  TGATCA               T^GATCA
MaeI          980  CTAG                 C^TAG
MvnI           86  CGCG                 CG^CG
NaeI           26  GCCGGC               GCC^GGC
NgoMIV         26  GCCGGC               G^CCGGC
NspI          366  GCATGT               [GA]CATG^[CT]
PflMI         756  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          178  CTGCAG               CTGCA^G
PvuII         175  CAGCTG               CAG^CTG
SacI          236  GAGCTC               GAGCT^C
SapI           21  GAAGAGC              GCTCTTC(1/4)
SfcI          178  CTGCAG               C^T[GA][CT]AG
SspI          781  AATATT               AAT^ATT
TaqI          864  TCGA                 T^CGA
TthHB8I       864  TCGA                 T^CGA
Van91I        756  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1170  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          392  GTAC                 GT^AC
              491  GTAC                
AlwNI         181  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              757  CAGAACCTG           
AspHI         236  GAGCTC               G[AT]GC[AT]^C
              541  GAGCAC              
BalI           98  TGGCCA               TGG^CCA
              371  TGGCCA              
BbsI          673  GAAGAC               GAAGAC(2/6)
             1170  GAAGAC              
BglI           26  GCCGGCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              373  GCCAGTTTGGC         
BpmI          595  CTCCAG               CTGGAG(16/14)
             1099  CTGGAG              
BpuAI         673  GAAGAC               GAAGAC(2/6)
             1170  GAAGAC              
BsiEI          28  CGGCCG               CG[GA][CT]^CG
              118  CGGCCG              
BsiHKAI       236  GAGCTC               G[AT]GC[AT]^C
              541  GAGCAC              
BspMI         585  ACCTGC               ACCTGC(4/8)
             1155  GCAGGT              
DdeI          576  CTCAG                C^T[ACGT]AG
              682  CTGAG               
DraII          92  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              182  AGGCCCT             
EagI           28  CGGCCG               C^GGCCG
              118  CGGCCG              
EclXI          28  CGGCCG               C^GGCCG
              118  CGGCCG              
Eco52I         28  CGGCCG               C^GGCCG
              118  CGGCCG              
EcoO109I       92  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              182  AGGCCCT             
MluNI          98  TGGCCA               TGG^CCA
              371  TGGCCA              
MscI           98  TGGCCA               TGG^CCA
              371  TGGCCA              
MslI          619  CATCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1161  CACCTTGGTG          
NlaIII        306  CATG                 CATG^
              367  CATG                
PleI          667  GACTC                GAGTC(4/5)
              124  GAGTC               
RsaI          392  GTAC                 GT^AC
              491  GTAC                
SmlI          277  CTCAAG               C^T[CT][GA]AG
              850  CTTGAG              
Tsp45I       1021  GTCAC                ^GT[GC]AC
             1159  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AluI        BanI        BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DpnII       EaeI        EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MfeI        MnlI        MseI        MspA1I      
MspI        MunI        MvaI        MwoI        NciI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       
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