Restriction map for pF1KA0459
 (Linear) (Six-base) MAPSORT of: KIAA0459.seq  Check: 5441  from: 1  to: 1194



KIAA0459.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     594    1194 
   Size:        594     600
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     692     892    1194 
   Size:        214     478     200     302

  Fragments arranged by size: 

                 478     302     214     200
 
AleI CACnn'nnGTG

Cuts at:      0    1165    1194 
   Size:       1165      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     186     762    1194 
   Size:        186     576     432

  Fragments arranged by size: 

                 576     432     186
 
ApaI G_GGCC'C

Cuts at:      0      96    1194 
   Size:         96    1098
 
AscI GG'CGCG_CC

Cuts at:      0      85    1194 
   Size:         85    1109
 
BanI G'GyrC_C

Cuts at:      0     207     582     910    1082    1194 
   Size:        207     375     328     172     112

  Fragments arranged by size: 

                 375     328     207     172     112
 
BanII G_rGCy'C

Cuts at:      0      96     240     857    1194 
   Size:         96     144     617     337

  Fragments arranged by size: 

                 617     337     144      96
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     680    1177    1194 
   Size:        680     497      17

  Fragments arranged by size: 

                 680     497      17
 
BclI T'GATC_A

Cuts at:      0     246    1194 
   Size:        246     948
 
BglI GCCn_nnn'nGGC

Cuts at:      0      32     379    1194 
   Size:         32     347     815

  Fragments arranged by size: 

                 815     347      32
 
Bme1580I G_kGCm'C

Cuts at:      0      96    1194 
   Size:         96    1098
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     851    1194 
   Size:        851     343
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     279    1194 
   Size:        279     915
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1120    1194 
   Size:        580     540      74

  Fragments arranged by size: 

                 580     540      74
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     871    1194 
   Size:        262     609     323

  Fragments arranged by size: 

                 609     323     262
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163     166     910    1090    1194 
   Size:        163       3     744     180     104

  Fragments arranged by size: 

                 744     180     163     104       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    1194 
   Size:        572     622
 
BsiEI CG_ry'CG

Cuts at:      0      31     121    1194 
   Size:         31      90    1073

  Fragments arranged by size: 

                1073      90      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     240     545    1194 
   Size:        240     305     649

  Fragments arranged by size: 

                 649     305     240
 
Bsp1286I G_dGCh'C

Cuts at:      0      96     240     545     857    1194 
   Size:         96     144     305     312     337

  Fragments arranged by size: 

                 337     312     305     144      96
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     594    1146    1194 
   Size:        594     552      48

  Fragments arranged by size: 

                 594     552      48
 
BsrBI CCG'CTC

Cuts at:      0      78     108    1183    1194 
   Size:         78      30    1075      11

  Fragments arranged by size: 

                1075      78      30      11
 
BsrDI GCAATG_nn'

Cuts at:      0     411    1194 
   Size:        411     783
 
BsrFI r'CCGG_y

Cuts at:      0      26    1194 
   Size:         26    1168
 
BssHII G'CGCG_C

Cuts at:      0      85    1194 
   Size:         85    1109
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     799    1194 
   Size:        799     395
 
BstEII G'GTnAC_C

Cuts at:      0    1158    1194 
   Size:       1158      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     507    1194 
   Size:        507     687
 
BtsI GCAGTG_nn'

Cuts at:      0     883     912    1054    1194 
   Size:        883      29     142     140

  Fragments arranged by size: 

                 883     142     140      29
 
EaeI y'GGCC_r

Cuts at:      0      28      98     118     371    1194 
   Size:         28      70      20     253     823

  Fragments arranged by size: 

                 823     253      70      28      20
 
EagI C'GGCC_G

Cuts at:      0      28     118    1194 
   Size:         28      90    1076

  Fragments arranged by size: 

                1076      90      28
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     820     836    1194 
   Size:         16     804      16     358

  Fragments arranged by size: 

                 804     358      16      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     126    1194 
   Size:        126    1068
 
EcoICRI GAG'CTC

Cuts at:      0     238    1194 
   Size:        238     956
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     580     692     892    1120    1194 
   Size:        214     366     112     200     228      74

  Fragments arranged by size: 

                 366     228     214     200     112      74
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     100    1194 
   Size:        100    1094
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     183    1194 
   Size:         93      90    1011

  Fragments arranged by size: 

                1011      93      90
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     865    1194 
   Size:        865     329
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     603     632     635     664    1194 
   Size:        603      29       3      29     530

  Fragments arranged by size: 

                 603     530      29      29       3
 
MfeI C'AATT_G

Cuts at:      0     222     250     413    1194 
   Size:        222      28     163     781

  Fragments arranged by size: 

                 781     222     163      28
 
MscI TGG'CCA

Cuts at:      0     100     373    1194 
   Size:        100     273     821

  Fragments arranged by size: 

                 821     273     100
 
MslI CAynn'nnrTG

Cuts at:      0     623    1165    1194 
   Size:        623     542      29

  Fragments arranged by size: 

                 623     542      29
 
MspA1I CmG'CkG

Cuts at:      0      38     177     318    1194 
   Size:         38     139     141     876

  Fragments arranged by size: 

                 876     141     139      38
 
NaeI GCC'GGC

Cuts at:      0      28    1194 
   Size:         28    1166
 
NgoMIV G'CCGG_C

Cuts at:      0      26    1194 
   Size:         26    1168
 
NspI r_CATG'y

Cuts at:      0     370    1194 
   Size:        370     824
 
PasI CC'CwG_GG

Cuts at:      0     186    1194 
   Size:        186    1008
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     762    1194 
   Size:        762     432
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171    1194 
   Size:        139      32    1023

  Fragments arranged by size: 

                1023     139      32
 
PsiI TTA'TAA

Cuts at:      0     779    1194 
   Size:        779     415
 
PspOMI G'GGCC_C

Cuts at:      0      92    1194 
   Size:         92    1102
 
PstI C_TGCA'G

Cuts at:      0     182    1194 
   Size:        182    1012
 
PvuII CAG'CTG

Cuts at:      0     177    1194 
   Size:        177    1017
 
SacI G_AGCT'C

Cuts at:      0     240    1194 
   Size:        240     954
 
SapI GCTCTTCn'nnn_

Cuts at:      0      16    1194 
   Size:         16    1178
 
SfcI C'TryA_G

Cuts at:      0     178    1194 
   Size:        178    1016
 
SmlI C'TyrA_G

Cuts at:      0     277     850    1194 
   Size:        277     573     344

  Fragments arranged by size: 

                 573     344     277
 
SspI AAT'ATT

Cuts at:      0     783    1194 
   Size:        783     411
 
StyI C'CwwG_G

Cuts at:      0     101     819    1163    1194 
   Size:        101     718     344      31

  Fragments arranged by size: 

                 718     344     101      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     967    1194 
   Size:        967     227
 
XmnI GAAnn'nnTTC

Cuts at:      0    1174    1194 
   Size:       1174      20
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApaI     AscI     BanI    BanII
     BbsI     BclI     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI
    BseRI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI   BssHII   BstAPI   BstEII    BtgZI     BtsI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
     MfeI     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI
    PflMI     PpiI     PsiI   PspOMI     PstI    PvuII     SacI     SapI
     SfcI     SmlI     SspI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
    BglII     BlpI    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrGI    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PfoI     PmeI     PmlI    PpuMI
    PshAI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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