Restriction map for pF1KA0455
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        Aor51HI     ApaI        
AscI        Asp718I     AviII       AvrII       BalI        
BamHI       BbrPI       BciVI       BfrI        BglII       
BlnI        BsaAI       BsaBI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BsrGI       BssSI       Bst1107I    
BstBI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoT22I     FseI        
FspI        HincII      HindII      HpaI        KpnI        
Ksp632I     MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PstI        
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AhdI          518  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         701  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          481  ATTAAT               AT^TAAT
AsnI          481  ATTAAT               AT^TAAT
Asp700I       421  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         518  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI           49  GGCGCC               G^G[CT][GA]CC
BbeI           49  GGCGCC               GGCGC^C
BbvCI          84  CCTCAGC              CCTCAGC(-5/-2)
BcgI          139  GCAGGGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          176  TGATCA               T^GATCA
BglI         1548  GCCTGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          375  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      375  GCTTAGC              GC^T[ACGT]AGC
BsaWI         314  ACCGGA               [AT]^CCGG[AT]
BsgI         1806  CTGCAC               GTGCAG(16/14)
BsmI         1301  GCATTC               GAATGC(1/-1)
BspMI         396  ACCTGC               ACCTGC(4/8)
BsrBI        1322  CCGCTC               CCGCTC(-3/-3)
BsrDI        2075  GCAATG               GCAATG(2/0)
BssHII          6  GCGCGC               G^CGCGC
BstAPI        757  GCAACCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1230  GGTTACC              G^GT[ACGT]ACC
BstPI        1230  GGTTACC              G^GT[ACGT]ACC
BstXI        1482  CCATGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         375  GCTTAGC              GC^T[ACGT]AGC
DraII        1585  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1344  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           24  TGGCCG               [CT]^GGCC[GA]
Eam1105I      518  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1585  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1230  GGTTACC              G^GT[ACGT]ACC
EcoRI         425  GAATTC               G^AATTC
FbaI          176  TGATCA               T^GATCA
HindIII       826  AAGCTT               A^AGCTT
KasI           49  GGCGCC               G^GCGCC
KspI           27  CCGCGG               CCGC^GG
NarI           49  GGCGCC               GG^CGCC
NheI         1147  GCTAGC               G^CTAGC
PpuMI        1585  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         313  GACCGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         481  ATTAAT               AT^TAAT
SacI         1984  GAGCTC               GAGCT^C
SacII          27  CCGCGG               CCGC^GG
SfoI           49  GGCGCC               GGC^GCC
XmnI          421  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI           49  GGCGCC               G[GA]^CG[CT]C
             1024  GACGCC              
AocI         1029  CCTCAGG              CC^T[ACGT]AGG
             1609  CCTGAGG             
BsaHI          49  GGCGCC               G[GA]^CG[CT]C
             1024  GACGCC              
BsmBI        1022  GAGACG               CGTCTC(1/5)
             2146  GAGACG              
Bsu36I       1029  CCTCAGG              CC^T[ACGT]AGG
             1609  CCTGAGG             
Eco81I       1029  CCTCAGG              CC^T[ACGT]AGG
             1609  CCTGAGG             
EcoRV         580  GATATC               GAT^ATC
              602  GATATC              
EcoT14I      1211  CCATGG               C^C[AT][AT]GG
             1482  CCATGG              
Hin1I          49  GGCGCC               G[GA]^CG[CT]C
             1024  GACGCC              
NcoI         1211  CCATGG               C^CATGG
             1482  CCATGG              
SfcI          563  CTACAG               C^T[GA][CT]AG
             1271  CTGTAG              
SmaI          109  CCCGGG               CCC^GGG
              127  CCCGGG              
SphI          326  GCATGC               GCATG^C
             1544  GCATGC              
StyI         1211  CCATGG               C^C[AT][AT]GG
             1482  CCATGG              
XcmI         1479  CCACCATGGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1842  CCATGAGCCCAGTGG     
XmaCI         109  CCCGGG               C^CCGGG
              127  CCCGGG              
XmaI          109  CCCGGG               C^CCGGG
              127  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        ApaLI       ApoI        AspHI       
AspI        AvaI        AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     XhoII       
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