Restriction map for pF1KA0443
 (Linear) (Six-base) MAPSORT of: KIAA0443.seq  Check: 6425  from: 1  to: 4185



KIAA0443.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AccI GT'mk_AC

Cuts at:      0     584    1278    2658    4185 
   Size:        584     694    1380    1527

  Fragments arranged by size: 

                1527    1380     694     584
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    1343    1742    2249    2528    2663    2723    2945 
   Size:       1097     246     399     507     279     135      60     222

Cuts at:   2945    3289    3479    3968    4185 
   Size:        344     190     489     217

  Fragments arranged by size: 

                1097     507     489     399     344     279     246     222
                 217     190     135      60
 
AflIII A'CryG_T

Cuts at:      0    3085    4185 
   Size:       3085    1100
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2784    4185 
   Size:       2784    1401
 
AlwNI CAG_nnn'CTG

Cuts at:      0     436    1720    2002    2317    2383    2701    3409    4185 
   Size:        436    1284     282     315      66     318     708     776

  Fragments arranged by size: 

                1284     776     708     436     318     315     282      66
            
 
ApaI G_GGCC'C

Cuts at:      0     824    4185 
   Size:        824    3361
 
ApoI r'AATT_y

Cuts at:      0     634     946    3371    3494    3903    3961    4120    4185 
   Size:        634     312    2425     123     409      58     159      65

  Fragments arranged by size: 

                2425     634     409     312     159     123      65      58
            
 
AseI AT'TA_AT

Cuts at:      0    3484    4185 
   Size:       3484     701
 
AvaI C'yCGr_G

Cuts at:      0    1166    3563    3629    4185 
   Size:       1166    2397      66     556

  Fragments arranged by size: 

                2397    1166     556      66
 
AvrII C'CTAG_G

Cuts at:      0     532    4185 
   Size:        532    3653
 
BamHI G'GATC_C

Cuts at:      0    3501    4185 
   Size:       3501     684
 
BanI G'GyrC_C

Cuts at:      0      25    4185 
   Size:         25    4160
 
BanII G_rGCy'C

Cuts at:      0     288     734     824    1832    4185 
   Size:        288     446      90    1008    2353

  Fragments arranged by size: 

                2353    1008     446     288      90
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     911    2405    4185 
   Size:        911    1494    1780

  Fragments arranged by size: 

                1780    1494     911
 
BbvCI CC'TCA_GC

Cuts at:      0    1442    1535    1817    2003    2318    4185 
   Size:       1442      93     282     186     315    1867

  Fragments arranged by size: 

                1867    1442     315     282     186      93
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     998    1032    4185 
   Size:        998      34    3153

  Fragments arranged by size: 

                3153     998      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2066    2787    4185 
   Size:       2066     721    1398

  Fragments arranged by size: 

                2066    1398     721
 
BclI T'GATC_A

Cuts at:      0    1109    3312    4185 
   Size:       1109    2203     873

  Fragments arranged by size: 

                2203    1109     873
 
BlpI GC'TnA_GC

Cuts at:      0     287    4185 
   Size:        287    3898
 
Bme1580I G_kGCm'C

Cuts at:      0      30     222     824    4185 
   Size:         30     192     602    3361

  Fragments arranged by size: 

                3361     602     192      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      14    1355    1445    4185 
   Size:         14    1341      90    2740

  Fragments arranged by size: 

                2740    1341      90      14
 
BmtI G_CTAG'C

Cuts at:      0    1355    2410    4185 
   Size:       1355    1055    1775

  Fragments arranged by size: 

                1775    1355    1055
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     348    1467    4185 
   Size:        348    1119    2718

  Fragments arranged by size: 

                2718    1119     348
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     630    1688    1924    2225    2375    4185 
   Size:        200     430    1058     236     301     150    1810

  Fragments arranged by size: 

                1810    1058     430     301     236     200     150
 
Bpu10I CC'TnA_GC

Cuts at:      0    1442    1535    1817    2003    2318    4127    4185 
   Size:       1442      93     282     186     315    1809      58

  Fragments arranged by size: 

                1809    1442     315     282     186      93      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     499    1537    2002    2230    2452    4185 
   Size:        499    1038     465     228     222    1733

  Fragments arranged by size: 

                1733    1038     499     465     228     222
 
BsaAI yAC'GTr

Cuts at:      0    3086    4185 
   Size:       3086    1099
 
BsaWI w'CCGG_w

Cuts at:      0    2559    4185 
   Size:       2559    1626
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     608     638     907     937    1279    1309    1661    1691 
   Size:        608      30     269      30     342      30     352      30

Cuts at:   1691    4185 
   Size:       2494

  Fragments arranged by size: 

                2494     608     352     342     269      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     517    1213    1834    1837    1840    2020    2107    2248 
   Size:        517     696     621       3       3     180      87     141

Cuts at:   2248    2335    2620    3151    4185 
   Size:         87     285     531    1034

  Fragments arranged by size: 

                1034     696     621     531     517     285     180     141
                  87      87       3       3
 
BseYI C'CCAG_C

Cuts at:      0     967    2476    4185 
   Size:        967    1509    1709

  Fragments arranged by size: 

                1709    1509     967
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227    4185 
   Size:        227    3958
 
BsiHKAI G_wGCw'C

Cuts at:      0     734    4185 
   Size:        734    3451
 
BsmI GAATG_Cn'

Cuts at:      0    4102    4185 
   Size:       4102      83
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     222     288     734     824    1832    4185 
   Size:         30     192      66     446      90    1008    2353

  Fragments arranged by size: 

                2353    1008     446     192      90      66      30
 
BspEI T'CCGG_A

Cuts at:      0    2559    4185 
   Size:       2559    1626
 
BspHI T'CATG_A

Cuts at:      0    3513    3923    4185 
   Size:       3513     410     262

  Fragments arranged by size: 

                3513     410     262
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     833    3068    4185 
   Size:        833    2235    1117

  Fragments arranged by size: 

                2235    1117     833
 
BsrDI GCAATG_nn'

Cuts at:      0    1142    3539    3870    4185 
   Size:       1142    2397     331     315

  Fragments arranged by size: 

                2397    1142     331     315
 
BsrFI r'CCGG_y

Cuts at:      0    2062    4185 
   Size:       2062    2123
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     681    3264    4185 
   Size:        681    2583     921

  Fragments arranged by size: 

                2583     921     681
 
BstYI r'GATC_y

Cuts at:      0     670     831     892    2668    3501    4185 
   Size:        670     161      61    1776     833     684

  Fragments arranged by size: 

                1776     833     684     670     161      61
 
BstZ17I GTA'TAC

Cuts at:      0    2659    4185 
   Size:       2659    1526
 
BtgI C'CryG_G

Cuts at:      0    3166    4185 
   Size:       3166    1019
 
BtsI GCAGTG_nn'

Cuts at:      0     215     626    2573    3959    4185 
   Size:        215     411    1947    1386     226

  Fragments arranged by size: 

                1947    1386     411     226     215
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     191     226    4185 
   Size:        191      35    3959

  Fragments arranged by size: 

                3959     191      35
 
EaeI y'GGCC_r

Cuts at:      0     683    1580    2861    4185 
   Size:        683     897    1281    1324

  Fragments arranged by size: 

                1324    1281     897     683
 
EarI CTCTTCn'nnn_

Cuts at:      0      53    1142    1322    1511    1535    1634    1697    1721 
   Size:         53    1089     180     189      24      99      63      24

Cuts at:   1721    1910    1943    2093    2177    2225    2294    2354    2450 
   Size:        189      33     150      84      48      69      60      96

Cuts at:   2450    2463    2504    2543    2699    2792    3455    3980    4185 
   Size:         13      41      39     156      93     663     525     205

  Fragments arranged by size: 

                1089     663     525     205     189     189     180     156
                 150      99      96      93      84      69      63      60
                  53      48      41      39      33      24      24      13
            
 
EcoICRI GAG'CTC

Cuts at:      0     732    4185 
   Size:        732    3453
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     630    1097    1343    1688    1742    1924    2225 
   Size:        200     430     467     246     345      54     182     301

Cuts at:   2225    2249    2375    2528    2663    2723    2945    3289    3479 
   Size:         24     126     153     135      60     222     344     190

Cuts at:   3479    3968    4185 
   Size:        489     217

  Fragments arranged by size: 

                 489     467     430     345     344     301     246     222
                 217     200     190     182     153     135     126      60
                  54      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     406    1098    4185 
   Size:        406     692    3087

  Fragments arranged by size: 

                3087     692     406
 
EcoO109I rG'GnC_Cy

Cuts at:      0     122     518     713    1217    1742    1841    2162    2435 
   Size:        122     396     195     504     525      99     321     273

Cuts at:   2435    2531    2627    2723    3008    4185 
   Size:         96      96      96     285    1177

  Fragments arranged by size: 

                1177     525     504     396     321     285     273     195
                 122      99      96      96      96
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2043    3493    4185 
   Size:       2043    1450     692

  Fragments arranged by size: 

                2043    1450     692
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     270     302     608     640     751     783 
   Size:          4      32     234      32     306      32     111      32

Cuts at:    783    1279    1311    2464    2496    3208    3240    3280    3312 
   Size:        496      32    1153      32     712      32      40      32

Cuts at:   3312    3403    3435    3771    3803    4185 
   Size:         91      32     336      32     382

  Fragments arranged by size: 

                1153     712     496     382     336     306     234     111
                  91      40      32      32      32      32      32      32
                  32      32      32      32       4
 
HincII GTy'rAC

Cuts at:      0    1524    1887    4185 
   Size:       1524     363    2298

  Fragments arranged by size: 

                2298    1524     363
 
HindIII A'AGCT_T

Cuts at:      0    1013    1865    4185 
   Size:       1013     852    2320

  Fragments arranged by size: 

                2320    1013     852
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2056    4185 
   Size:       2056    2129
 
MscI TGG'CCA

Cuts at:      0     685    2863    4185 
   Size:        685    2178    1322

  Fragments arranged by size: 

                2178    1322     685
 
MslI CAynn'nnrTG

Cuts at:      0     679    3708    4185 
   Size:        679    3029     477

  Fragments arranged by size: 

                3029     679     477
 
MspA1I CmG'CkG

Cuts at:      0    1471    1610    2380    2413    3762    4185 
   Size:       1471     139     770      33    1349     423

  Fragments arranged by size: 

                1471    1349     770     423     139      33
 
NcoI C'CATG_G

Cuts at:      0    3166    4185 
   Size:       3166    1019
 
NdeI CA'TA_TG

Cuts at:      0    3709    4185 
   Size:       3709     476
 
NheI G'CTAG_C

Cuts at:      0    1351    2406    4185 
   Size:       1351    1055    1779

  Fragments arranged by size: 

                1779    1351    1055
 
PasI CC'CwG_GG

Cuts at:      0     824    4185 
   Size:        824    3361
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     812    1936    4185 
   Size:        812    1124    2249

  Fragments arranged by size: 

                2249    1124     812
 
PfoI T'CCnGG_A

Cuts at:      0    1618    2971    3365    4185 
   Size:       1618    1353     394     820

  Fragments arranged by size: 

                1618    1353     820     394
 
PmlI CAC'GTG

Cuts at:      0    3086    4185 
   Size:       3086    1099
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2803    2835    4185 
   Size:       2803      32    1350

  Fragments arranged by size: 

                2803    1350      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     122     518     713    1217    1742    1841    2162    2435 
   Size:        122     396     195     504     525      99     321     273

Cuts at:   2435    2531    2627    2723    3008    4185 
   Size:         96      96      96     285    1177

  Fragments arranged by size: 

                1177     525     504     396     321     285     273     195
                 122      99      96      96      96
 
PshAI GACnn'nnGTC

Cuts at:      0    1975    4185 
   Size:       1975    2210
 
PspOMI G'GGCC_C

Cuts at:      0     820    4185 
   Size:        820    3365
 
PvuII CAG'CTG

Cuts at:      0    1471    1610    2380    2413    3762    4185 
   Size:       1471     139     770      33    1349     423

  Fragments arranged by size: 

                1471    1349     770     423     139      33
 
SacI G_AGCT'C

Cuts at:      0     734    4185 
   Size:        734    3451
 
ScaI AGT'ACT

Cuts at:      0     369    4185 
   Size:        369    3816
 
SexAI A'CCwGG_T

Cuts at:      0     811    1219    4185 
   Size:        811     408    2966

  Fragments arranged by size: 

                2966     811     408
 
SfcI C'TryA_G

Cuts at:      0    1040    1232    2177    3354    3857    4185 
   Size:       1040     192     945    1177     503     328

  Fragments arranged by size: 

                1177    1040     945     503     328     192
 
SspI AAT'ATT

Cuts at:      0    1736    4002    4022    4032    4083    4185 
   Size:       1736    2266      20      10      51     102

  Fragments arranged by size: 

                2266    1736     102      51      20      10
 
StuI AGG'CCT

Cuts at:      0     537    3123    3971    4185 
   Size:        537    2586     848     214

  Fragments arranged by size: 

                2586     848     537     214
 
StyI C'CwwG_G

Cuts at:      0     113     167     239     532    1160    1369    3166    3392 
   Size:        113      54      72     293     628     209    1797     226

Cuts at:   3392    4185 
   Size:        793

  Fragments arranged by size: 

                1797     793     628     293     226     209     113      72
                  54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     202    4185 
   Size:        202    3983
 
TatI w'GTAC_w

Cuts at:      0     367    3400    4185 
   Size:        367    3033     785

  Fragments arranged by size: 

                3033     785     367
 
Tth111I GACn'n_nGTC

Cuts at:      0    1626    2107    4185 
   Size:       1626     481    2078

  Fragments arranged by size: 

                2078    1626     481
 
XbaI T'CTAG_A

Cuts at:      0    2827    4185 
   Size:       2827    1358
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     139    1281    4185 
   Size:        139    1142    2904

  Fragments arranged by size: 

                2904    1142     139
 
XmnI GAAnn'nnTTC

Cuts at:      0    3494    3549    4185 
   Size:       3494      55     636

  Fragments arranged by size: 

                3494     636      55
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI    AlwNI     ApaI     ApoI     AseI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI
    BstXI    BstYI  BstZ17I     BtgI     BtsI    CspCI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     PasI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PvuII
     SacI     ScaI    SexAI     SfcI     SspI     StuI     StyI    TaqII
     TatI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI    AsiSI     BaeI     BbeI    BfrBI
     BglI    BglII    BmgBI    BpuEI    BsaBI    BsaHI    BsiEI    BsiWI
    BsmBI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EciI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SwaI     TstI     XhoI     XmaI     ZraI
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