Restriction map for pF1KA0424
 (Linear) (Six-base) MAPSORT of: KIAA0424.seq  Check: 7789  from: 1  to: 1548



KIAA0424.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AatII G_ACGT'C

Cuts at:      0     101    1548 
   Size:        101    1447
 
AccI GT'mk_AC

Cuts at:      0     775    1548 
   Size:        775     773
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     454    1506    1548 
   Size:        409      45    1052      42

  Fragments arranged by size: 

                1052     409      45      42
 
AflIII A'CryG_T

Cuts at:      0     413    1548 
   Size:        413    1135
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1440    1548 
   Size:       1440     108
 
ApaI G_GGCC'C

Cuts at:      0     227    1548 
   Size:        227    1321
 
ApoI r'AATT_y

Cuts at:      0    1307    1548 
   Size:       1307     241
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1195    1228    1548 
   Size:       1195      33     320

  Fragments arranged by size: 

                1195     320      33
 
BanII G_rGCy'C

Cuts at:      0     227     623     767     949    1548 
   Size:        227     396     144     182     599

  Fragments arranged by size: 

                 599     396     227     182     144
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1008    1548 
   Size:       1008     540
 
BbvCI CC'TCA_GC

Cuts at:      0      38     529    1548 
   Size:         38     491    1019

  Fragments arranged by size: 

                1019     491      38
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     755    1548 
   Size:        755     793
 
BclI T'GATC_A

Cuts at:      0      11    1548 
   Size:         11    1537
 
BglII A'GATC_T

Cuts at:      0     734    1548 
   Size:        734     814
 
BlpI GC'TnA_GC

Cuts at:      0     761    1548 
   Size:        761     787
 
Bme1580I G_kGCm'C

Cuts at:      0     227    1548 
   Size:        227    1321
 
BmgBI CAC'GTC

Cuts at:      0      57    1548 
   Size:         57    1491
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     927     974    1548 
   Size:        927      47     574

  Fragments arranged by size: 

                 927     574      47
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     517     707    1249    1548 
   Size:         38     479     190     542     299

  Fragments arranged by size: 

                 542     479     299     190      38
 
Bpu10I CC'TnA_GC

Cuts at:      0      38     529    1249    1358    1548 
   Size:         38     491     720     109     190

  Fragments arranged by size: 

                 720     491     190     109      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349    1548 
   Size:        349    1199
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     485    1548 
   Size:        485    1063
 
BsaHI Gr'CG_yC

Cuts at:      0      98    1548 
   Size:         98    1450
 
BsaWI w'CCGG_w

Cuts at:      0    1064    1548 
   Size:       1064     484
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     151     181     490     520    1548 
   Size:        151      30     309      30    1028

  Fragments arranged by size: 

                1028     309     151      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     232    1213    1548 
   Size:        172      60     981     335

  Fragments arranged by size: 

                 981     335     172      60
 
BseYI C'CCAG_C

Cuts at:      0     226    1548 
   Size:        226    1322
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     748    1548 
   Size:        256     492     800

  Fragments arranged by size: 

                 800     492     256
 
BsiEI CG_ry'CG

Cuts at:      0     999    1548 
   Size:        999     549
 
BsiHKAI G_wGCw'C

Cuts at:      0     340     560     623     767     949    1548 
   Size:        340     220      63     144     182     599

  Fragments arranged by size: 

                 599     340     220     182     144      63
 
BsmI GAATG_Cn'

Cuts at:      0    1173    1548 
   Size:       1173     375
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     340     560     623     767     949    1548 
   Size:        227     113     220      63     144     182     599

  Fragments arranged by size: 

                 599     227     220     182     144     113      63
 
BspEI T'CCGG_A

Cuts at:      0    1064    1548 
   Size:       1064     484
 
BspHI T'CATG_A

Cuts at:      0     827    1548 
   Size:        827     721
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1458    1517    1548 
   Size:       1458      59      31

  Fragments arranged by size: 

                1458      59      31
 
BsrBI CCG'CTC

Cuts at:      0    1385    1548 
   Size:       1385     163
 
BsrDI GCAATG_nn'

Cuts at:      0     695    1343    1548 
   Size:        695     648     205

  Fragments arranged by size: 

                 695     648     205
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1227    1548 
   Size:       1227     321
 
BstYI r'GATC_y

Cuts at:      0     734     980    1548 
   Size:        734     246     568

  Fragments arranged by size: 

                 734     568     246
 
BstZ17I GTA'TAC

Cuts at:      0     776    1548 
   Size:        776     772
 
BtgI C'CryG_G

Cuts at:      0      62    1428    1548 
   Size:         62    1366     120

  Fragments arranged by size: 

                1366     120      62
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     245    1440    1548 
   Size:        245    1195     108

  Fragments arranged by size: 

                1195     245     108
 
BtsI GCAGTG_nn'

Cuts at:      0     400    1548 
   Size:        400    1148
 
ClaI AT'CG_AT

Cuts at:      0     704    1548 
   Size:        704     844
 
DraI TTT'AAA

Cuts at:      0      87    1548 
   Size:         87    1461
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1153    1548 
   Size:       1153     395
 
EaeI y'GGCC_r

Cuts at:      0      65     283     996    1431    1548 
   Size:         65     218     713     435     117

  Fragments arranged by size: 

                 713     435     218     117      65
 
EagI C'GGCC_G

Cuts at:      0     996    1548 
   Size:        996     552
 
EarI CTCTTCn'nnn_

Cuts at:      0     397    1259    1318    1548 
   Size:        397     862      59     230

  Fragments arranged by size: 

                 862     397     230      59
 
EcoICRI GAG'CTC

Cuts at:      0     621     765     947    1548 
   Size:        621     144     182     601

  Fragments arranged by size: 

                 621     601     182     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     409     454     517     707    1249    1506    1548 
   Size:         38     371      45      63     190     542     257      42

  Fragments arranged by size: 

                 542     371     257     190      63      45      42      38
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     223     541    1056    1416    1548 
   Size:        223     318     515     360     132

  Fragments arranged by size: 

                 515     360     318     223     132
 
EcoRV GAT'ATC

Cuts at:      0     462    1548 
   Size:        462    1086
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     459     765    1188    1548 
   Size:        459     306     423     360

  Fragments arranged by size: 

                 459     423     360     306
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      11      43     931     963    1548 
   Size:         11      32     888      32     585

  Fragments arranged by size: 

                 888     585      32      32      11
 
HincII GTy'rAC

Cuts at:      0    1122    1374    1532    1548 
   Size:       1122     252     158      16

  Fragments arranged by size: 

                1122     252     158      16
 
HindIII A'AGCT_T

Cuts at:      0    1177    1548 
   Size:       1177     371
 
HpaI GTT'AAC

Cuts at:      0    1532    1548 
   Size:       1532      16
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1548 
   Size:        146    1402
 
MscI TGG'CCA

Cuts at:      0      67    1548 
   Size:         67    1481
 
MslI CAynn'nnrTG

Cuts at:      0     563     605    1099    1548 
   Size:        563      42     494     449

  Fragments arranged by size: 

                 563     494     449      42
 
MspA1I CmG'CkG

Cuts at:      0     814    1011    1548 
   Size:        814     197     537

  Fragments arranged by size: 

                 814     537     197
 
NcoI C'CATG_G

Cuts at:      0      62    1548 
   Size:         62    1486
 
NspI r_CATG'y

Cuts at:      0     417    1548 
   Size:        417    1131
 
PciI A'CATG_T

Cuts at:      0     413    1548 
   Size:        413    1135
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1038    1440    1548 
   Size:       1038     402     108

  Fragments arranged by size: 

                1038     402     108
 
PpuMI rG'GwC_Cy

Cuts at:      0     541    1056    1416    1548 
   Size:        541     515     360     132

  Fragments arranged by size: 

                 541     515     360     132
 
PspOMI G'GGCC_C

Cuts at:      0     223    1548 
   Size:        223    1325
 
PvuII CAG'CTG

Cuts at:      0     814    1548 
   Size:        814     734
 
SacI G_AGCT'C

Cuts at:      0     623     767     949    1548 
   Size:        623     144     182     599

  Fragments arranged by size: 

                 623     599     182     144
 
SexAI A'CCwGG_T

Cuts at:      0    1439    1548 
   Size:       1439     109
 
SfcI C'TryA_G

Cuts at:      0     289     465     798    1548 
   Size:        289     176     333     750

  Fragments arranged by size: 

                 750     333     289     176
 
SmlI C'TyrA_G

Cuts at:      0     364    1548 
   Size:        364    1184
 
SspI AAT'ATT

Cuts at:      0     879    1548 
   Size:        879     669
 
StuI AGG'CCT

Cuts at:      0     667    1548 
   Size:        667     881
 
StyI C'CwwG_G

Cuts at:      0      62    1548 
   Size:         62    1486
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     183    1548 
   Size:        183    1365
 
TatI w'GTAC_w

Cuts at:      0    1079    1548 
   Size:       1079     469
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     121     153    1548 
   Size:        121      32    1395

  Fragments arranged by size: 

                1395     121      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      75    1548 
   Size:         75    1473
 
ZraI GAC'GTC

Cuts at:      0      99    1548 
   Size:         99    1449
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII    AlwNI     ApaI     ApoI     BaeI
    BanII     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I   HincII
  HindIII     HpaI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PciI    PflMI    PpuMI   PspOMI    PvuII     SacI    SexAI     SfcI
     SmlI     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI     BbeI     BcgI    BfrBI     BglI     BmtI     BplI
    BsaAI    BsaBI    BsiWI    BsmBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII   Bsu36I    CspCI   DraIII     EciI    EcoNI
    EcoRI     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI
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