Restriction map for pF1KA0358
##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        ApaI        
AscI        AviII       BbrPI       BciVI       BfrI        
BglII       BsaAI       BseAI       BsiEI       BsiWI       
BspEI       BssSI       BstBI       CpoI        EagI        
EclXI       Eco52I      EcoT22I     FseI        FspI        
KspI        MfeI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PflMI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Van91I      XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I       4052  GGTACC               G^GTACC
Alw44I       2845  GTGCAC               G^TGCAC
Aor51HI      4427  AGCGCT               AGC^GCT
ApaLI        2845  GTGCAC               G^TGCAC
AseI         1948  ATTAAT               AT^TAAT
AsnI         1948  ATTAAT               AT^TAAT
Asp718I      4052  GGTACC               G^GTACC
AvrII        3864  CCTAGG               C^CTAGG
BbeI         4190  GGCGCC               GGCGC^C
BcgI          723  GCATGACCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          617  GCCTTCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3864  CCTAGG               C^CTAGG
BlpI          537  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      537  GCTCAGC              GC^T[ACGT]AGC
BsaBI        4096  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        4076  ACCGGA               [AT]^CCGG[AT]
BspDI         755  ATCGAT               AT^CGAT
BspHI        1344  TCATGA               T^CATGA
BspLU11I     2394  ACATGT               A^CATGT
BssHII       4581  GCGCGC               G^CGCGC
Bst1107I      579  GTATAC               GTA^TAC
BstEII       4680  GGTGACC              G^GT[ACGT]ACC
BstPI        4680  GGTGACC              G^GT[ACGT]ACC
BstZ17I       579  GTATAC               GTA^TAC
CelII         537  GCTCAGC              GC^T[ACGT]AGC
ClaI          755  ATCGAT               AT^CGAT
Eco47III     4427  AGCGCT               AGC^GCT
EcoO65I      4680  GGTGACC              G^GT[ACGT]ACC
EcoRV        4360  GATATC               GAT^ATC
HincII        283  GTTAAC               GT[CT]^[GA]AC
HindII        283  GTTAAC               GT[CT]^[GA]AC
HindIII      3398  AAGCTT               A^AGCTT
HpaI          283  GTTAAC               GTT^AAC
KasI         4190  GGCGCC               G^GCGCC
KpnI         4052  GGTACC               GGTAC^C
MamI         4096  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1528  ACGCGT               A^CGCGT
NarI         4190  GGCGCC               GG^CGCC
NcoI         1475  CCATGG               C^CATGG
PaeR7I        644  CTCGAG               C^TCGAG
PshBI        1948  ATTAAT               AT^TAAT
RcaI         1344  TCATGA               T^CATGA
SexAI        3666  ACCAGGT              A^CC[AT]GGT
SfoI         4190  GGCGCC               GGC^GCC
SgrAI        2290  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         2698  CCCGGG               CCC^GGG
XhoI          644  CTCGAG               C^TCGAG
XmaCI        2698  CCCGGG               C^CCGGG
XmaI         2698  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AatII        2258  GACGTC               GACGT^C
             3501  GACGTC              
AccI          579  GTATAC               GT^[AC][GT]AC
              799  GTAGAC              
AflIII       1528  ACGCGT               A^C[GA][CT]GT
             2394  ACATGT              
AhdI         1390  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4458  GACCTACTGTC         
AocI         2341  CCTGAGG              CC^T[ACGT]AGG
             3307  CCTAAGG             
Asp700I        12  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1376  GAAGGCCTTC          
AspEI        1390  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4458  GACCTACTGTC         
AspI         1918  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3529  GACTCTGTC           
BbsI         4653  GAAGAC               GAAGAC(2/6)
              232  GTCTTC              
BbvCI        3057  CCTCAGC              CCTCAGC(-5/-2)
             1804  GCTGAGG             
BclI         3522  TGATCA               T^GATCA
             4251  TGATCA              
BpuAI        4653  GAAGAC               GAAGAC(2/6)
              232  GTCTTC              
BsmI          944  GCATTC               GAATGC(1/-1)
             1012  GCATTC              
BsrDI        1021  GCAATG               GCAATG(2/0)
             3650  GCAATG              
BstAPI       2847  GCACAGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3283  GCACCAAGTGC         
Bsu36I       2341  CCTGAGG              CC^T[ACGT]AGG
             3307  CCTAAGG             
DraI         1281  TTTAAA               TTT^AAA
             3330  TTTAAA              
DrdI          253  GACACTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              802  GACATCGAGGTC        
Eam1105I     1390  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4458  GACCTACTGTC         
Eco81I       2341  CCTGAGG              CC^T[ACGT]AGG
             3307  CCTAAGG             
EcoNI        2832  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4671  CCTGCTGCAGG         
EcoRI        1414  GAATTC               G^AATTC
             1694  GAATTC              
FbaI         3522  TGATCA               T^GATCA
             4251  TGATCA              
PflFI        1918  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3529  GACTCTGTC           
SacI          402  GAGCTC               GAGCT^C
              823  GAGCTC              
ScaI          514  AGTACT               AGT^ACT
             1646  AGTACT              
Tth111I      1918  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3529  GACTCTGTC           
XmnI           12  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1376  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PpuMI       
PshAI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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