Restriction map for pF1KA0354
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BcgI        
BciVI       BcnI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BstEII      BstPI       BstXI       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRI       
EcoT22I     FseI        HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SspBI       SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          252  GTATAC               GT^[AC][GT]AC
AccII         394  CGCG                 CG^CG
AcsI         1230  GAATTT               [GA]^AATT[CT]
AcyI          563  GACGCC               G[GA]^CG[CT]C
Aor51HI       422  AGCGCT               AGC^GCT
ApaI         1369  GGGCCC               GGGCC^C
ApoI         1230  GAATTT               [GA]^AATT[CT]
AvaI          683  CTCGGG               C^[CT]CG[GA]G
AviII         524  TGCGCA               TGC^GCA
BanI          813  GGTGCC               G^G[CT][GA]CC
BbvCI        1107  CCTCAGC              CCTCAGC(-5/-2)
BglI          482  GCCAGAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          464  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      464  GCTCAGC              GC^T[ACGT]AGC
BsaHI         563  GACGCC               G[GA]^CG[CT]C
BsaWI         654  ACCGGA               [AT]^CCGG[AT]
BsmBI         561  GAGACG               CGTCTC(1/5)
BsmFI         447  GGGAC                GGGAC(10/14)
BsoBI         683  CTCGGG               C^[CT]CG[GA]G
BsrDI         281  GCAATG               GCAATG(2/0)
BssSI         869  CTCGTG               CACGAG(-5/-1)
Bst1107I      252  GTATAC               GTA^TAC
BstAPI       1844  GCAAAATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1227  TTCGAA               TT^CGAA
BstUI         394  CGCG                 CG^CG
BstZ17I       252  GTATAC               GTA^TAC
CelII         464  GCTCAGC              GC^T[ACGT]AGC
DraI          103  TTTAAA               TTT^AAA
DsaI          860  CCATGG               C^C[GA][CT]GG
EarI          516  CTCTTC               CTCTTC(1/4)
Eco47III      422  AGCGCT               AGC^GCT
EcoNI        1547  CCTTCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1171  GATATC               GAT^ATC
FspI          524  TGCGCA               TGC^GCA
HapII         655  CCGG                 C^CGG
Hin1I         563  GACGCC               G[GA]^CG[CT]C
HincII       1749  GTTGAC               GT[CT]^[GA]AC
HindII       1749  GTTGAC               GT[CT]^[GA]AC
HpaII         655  CCGG                 C^CGG
Ksp632I       516  CTCTTC               CTCTTC(1/4)
MaeII        1702  ACGT                 A^CGT
MspI          655  CCGG                 C^CGG
MvnI          394  CGCG                 CG^CG
NcoI          860  CCATGG               C^CATGG
NspV         1227  TTCGAA               TT^CGAA
SapI          515  GCTCTTC              GCTCTTC(1/4)
SbfI         1360  CCTGCAGG             CCTGCA^GG
SfuI         1227  TTCGAA               TT^CGAA
SmlI          797  CTCAAG               C^T[CT][GA]AG
Sse8387I     1360  CCTGCAGG             CCTGCA^GG
SspI         1288  AATATT               AAT^ATT
TaqI         1228  TCGA                 T^CGA
TfiI          706  GATTC                G^A[AT]TC
TthHB8I      1228  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AocI          943  CCTGAGG              CC^T[ACGT]AGG
             1015  CCTCAGG             
BalI           60  TGGCCA               TGG^CCA
             1719  TGGCCA              
BclI          780  TGATCA               T^GATCA
              897  TGATCA              
BpmI         1551  CTCCAG               CTGGAG(16/14)
             1318  CTGGAG              
BsrBI         114  CCGCTC               CCGCTC(-3/-3)
              595  GAGCGG              
BstYI        1140  AGATCC               [GA]^GATC[CT]
             1655  AGATCC              
Bsu36I        943  CCTGAGG              CC^T[ACGT]AGG
             1015  CCTCAGG             
DraII        1328  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1369  GGGCCCT             
EaeI           60  TGGCCA               [CT]^GGCC[GA]
             1719  TGGCCA              
Eco81I        943  CCTGAGG              CC^T[ACGT]AGG
             1015  CCTCAGG             
EcoO109I     1328  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1369  GGGCCCT             
FauI          413  CCCGC                CCCGC(4/6)
              597  GCGGG               
FbaI          780  TGATCA               T^GATCA
              897  TGATCA              
HgaI          366  GACGC                GACGC(5/10)
              563  GACGC               
MflI         1140  AGATCC               [GA]^GATC[CT]
             1655  AGATCC              
MluNI          60  TGGCCA               TGG^CCA
             1719  TGGCCA              
MscI           60  TGGCCA               TGG^CCA
             1719  TGGCCA              
PflMI        1176  CCATATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1348  CCAGAGGCTGG         
SpeI          889  ACTAGT               A^CTAGT
             1303  ACTAGT              
SphI          130  GCATGC               GCATG^C
              416  GCATGC              
Van91I       1176  CCATATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1348  CCAGAGGCTGG         
XhoII        1140  AGATCC               [GA]^GATC[CT]
             1655  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
BanII       BbvI        BfaI        BmyI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XcmI        
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