Restriction map for pF1KA0330
##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AatII       AclI        AflII       AscI        AseI        
AsnI        AviII       AvrII       BbeI        BbrPI       
BfrI        BlnI        BsaAI       BsiWI       BspDI       
BsrDI       BssHII      Bst1107I    BstZ17I     ClaI        
CpoI        EcoRI       EcoRV       FseI        FspI        
KasI        KspI        MluI        NarI        NdeI        
NheI        NotI        NruI        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI         2974  GAATTT               [GA]^AATT[CT]
AgeI          149  ACCGGT               A^CCGGT
AhdI         5554  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2148  GTGCAC               G^TGCAC
Aor51HI       744  AGCGCT               AGC^GCT
ApaLI        2148  GTGCAC               G^TGCAC
ApoI         2974  GAATTT               [GA]^AATT[CT]
Asp700I      3378  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        5554  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI        4670  GTATCC               GTATCC(6/5)
BclI           45  TGATCA               T^GATCA
BglII        4272  AGATCT               A^GATCT
BsaBI        2373  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         3305  GCATTC               GAATGC(1/-1)
BspHI          48  TCATGA               T^CATGA
BstBI           5  TTCGAA               TT^CGAA
DraI           61  TTTAAA               TTT^AAA
DrdI         4930  GACCGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         4780  CGGCCG               C^GGCCG
Eam1105I     5554  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        4780  CGGCCG               C^GGCCG
Eco47III      744  AGCGCT               AGC^GCT
Eco52I       4780  CGGCCG               C^GGCCG
EcoT22I      3235  ATGCAT               ATGCA^T
FbaI           45  TGATCA               T^GATCA
HpaI          955  GTTAAC               GTT^AAC
MamI         2373  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3478  CAATTG               C^AATTG
MunI         3478  CAATTG               C^AATTG
NsiI         3235  ATGCAT               ATGCA^T
NspV            5  TTCGAA               TT^CGAA
PinAI         149  ACCGGT               A^CCGGT
RcaI           48  TCATGA               T^CATGA
ScaI         2754  AGTACT               AGT^ACT
SfuI            5  TTCGAA               TT^CGAA
SmaI         3293  CCCGGG               CCC^GGG
SspI          266  AATATT               AAT^ATT
XmaCI        3293  CCCGGG               C^CCGGG
XmaI         3293  CCCGGG               C^CCGGG
XmnI         3378  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I       2882  GGTACC               G^GTACC
             3117  GGTACC              
AccI         1222  GTAGAC               GT^[AC][GT]AC
             1540  GTCTAC              
AccIII        413  TCCGGA               T^CCGGA
              623  TCCGGA              
AcyI          948  GACGCC               G[GA]^CG[CT]C
             2643  GACGCC              
Asp718I      2882  GGTACC               G^GTACC
             3117  GGTACC              
BamHI         416  GGATCC               G^GATCC
             4757  GGATCC              
BcgI          194  CGAGCCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3714  CGAGGAGGCTGC        
BsaHI         948  GACGCC               G[GA]^CG[CT]C
             2643  GACGCC              
BseAI         413  TCCGGA               T^CCGGA
              623  TCCGGA              
BsiEI        4116  CGACCG               CG[GA][CT]^CG
             4780  CGGCCG              
BsmBI        2523  CGTCTC               CGTCTC(1/5)
             2641  GAGACG              
BspEI         413  TCCGGA               T^CCGGA
              623  TCCGGA              
BstEII       1771  GGTGACC              G^GT[ACGT]ACC
             6340  GGTCACC             
BstPI        1771  GGTGACC              G^GT[ACGT]ACC
             6340  GGTCACC             
EcoO65I      1771  GGTGACC              G^GT[ACGT]ACC
             6340  GGTCACC             
Hin1I         948  GACGCC               G[GA]^CG[CT]C
             2643  GACGCC              
KpnI         2882  GGTACC               GGTAC^C
             3117  GGTACC              
MroI          413  TCCGGA               T^CCGGA
              623  TCCGGA              
SapI         6046  GAAGAGC              GCTCTTC(1/4)
             4710  GCTCTTC             
SphI         3360  GCATGC               GCATG^C
             3545  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        SmlI        SspBI       StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XcmI        XhoII       
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