Restriction map for pF1KA0329
##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AccI        AflII       AgeI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AvrII       
BciVI       BclI        BfrI        BglII       BlnI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     DraI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FbaI        FseI        HpaI        KpnI        
KspI        MluI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshBI       PvuI        RcaI        
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        2247  GACGTC               GACGT^C
AccIII       2898  TCCGGA               T^CCGGA
AclI         1635  AACGTT               AA^CGTT
AflIII       2145  ACGTGT               A^C[GA][CT]GT
AhdI         1507  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1507  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3194  TGCGCA               TGC^GCA
BamHI         973  GGATCC               G^GATCC
BbeI         2515  GGCGCC               GGCGC^C
BsaBI        1070  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2898  TCCGGA               T^CCGGA
BspDI           6  ATCGAT               AT^CGAT
BspEI        2898  TCCGGA               T^CCGGA
BsrBI          93  CCGCTC               CCGCTC(-3/-3)
BsrDI         326  CATTGC               GCAATG(2/0)
BssHII       3513  GCGCGC               G^CGCGC
ClaI            6  ATCGAT               AT^CGAT
CpoI         3877  CGGACCG              CG^G[AT]CCG
DraIII       3142  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2241  GACATTGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1507  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        3286  GAATTC               G^AATTC
EcoRV           9  GATATC               GAT^ATC
FspI         3194  TGCGCA               TGC^GCA
HincII        943  GTCAAC               GT[CT]^[GA]AC
HindII        943  GTCAAC               GT[CT]^[GA]AC
KasI         2515  GGCGCC               G^GCGCC
MamI         1070  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          956  CAATTG               C^AATTG
MroI         2898  TCCGGA               T^CCGGA
MunI          956  CAATTG               C^AATTG
NarI         2515  GGCGCC               GG^CGCC
PflMI        2553  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     1635  AACGTT               AA^CGTT
RsrII        3877  CGGACCG              CG^G[AT]CCG
SacI         2317  GAGCTC               GAGCT^C
ScaI          637  AGTACT               AGT^ACT
SexAI         269  ACCTGGT              A^CC[AT]GGT
SfoI         2515  GGCGCC               GGC^GCC
SmaI          712  CCCGGG               CCC^GGG
SspI         1014  AATATT               AAT^ATT
Van91I       2553  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          471  TCTAGA               T^CTAGA
XmaCI         712  CCCGGG               C^CCGGG
XmaI          712  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Alw44I        999  GTGCAC               G^TGCAC
             3868  GTGCAC              
AocI         2573  CCTCAGG              CC^T[ACGT]AGG
             4201  CCTGAGG             
ApaLI         999  GTGCAC               G^TGCAC
             3868  GTGCAC              
BbrPI        1126  CACGTG               CAC^GTG
             3871  CACGTG              
BcgI          152  GCAGCAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2960  GCATAACGCTCG        
BlpI          252  GCTGAGC              GC^T[ACGT]AGC
             3954  GCTGAGC             
Bpu1102I      252  GCTGAGC              GC^T[ACGT]AGC
             3954  GCTGAGC             
BsaAI        1126  CACGTG               [CT]AC^GT[GA]
             3871  CACGTG              
BsmBI        1168  GAGACG               CGTCTC(1/5)
             2302  GAGACG              
BspMI        2556  GCAGGT               ACCTGC(4/8)
             3857  GCAGGT              
BsrFI         182  GCCGGC               [GA]^CCGG[CT]
             2351  GCCGGC              
Bsu36I       2573  CCTCAGG              CC^T[ACGT]AGG
             4201  CCTGAGG             
CelII         252  GCTGAGC              GC^T[ACGT]AGC
             3954  GCTGAGC             
Cfr10I        182  GCCGGC               [GA]^CCGG[CT]
             2351  GCCGGC              
EarI         1185  CTCTTC               CTCTTC(1/4)
             4173  GAAGAG              
Eco81I       2573  CCTCAGG              CC^T[ACGT]AGG
             4201  CCTGAGG             
Ksp632I      1185  CTCTTC               CTCTTC(1/4)
             4173  GAAGAG              
NaeI          182  GCCGGC               GCC^GGC
             2351  GCCGGC              
NcoI         1190  CCATGG               C^CATGG
             3003  CCATGG              
NgoMIV        182  GCCGGC               G^CCGGC
             2351  GCCGGC              
PmaCI        1126  CACGTG               CAC^GTG
             3871  CACGTG              
PmlI         1126  CACGTG               CAC^GTG
             3871  CACGTG              
PshAI        2113  GACACAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2175  GACTCCGGTC          
SapI         4173  GAAGAGC              GCTCTTC(1/4)
             1184  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XcmI        
XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com