Restriction map for pF1KA0329
 (Linear) (Six-base) MAPSORT of: KIAA0329.seq  Check: 2917  from: 1  to: 4233



KIAA0329.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2547    3848    4233 
   Size:       2547    1301     385

  Fragments arranged by size: 

                2547    1301     385
 
AatII G_ACGT'C

Cuts at:      0    2251    4233 
   Size:       2251    1982
 
AclI AA'CG_TT

Cuts at:      0    1636    4233 
   Size:       1636    2597
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614    1085    3205    4233 
   Size:        614     471    2120    1028

  Fragments arranged by size: 

                2120    1028     614     471
 
AflIII A'CryG_T

Cuts at:      0    2145    4233 
   Size:       2145    2088
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1512    4233 
   Size:       1512    2721
 
AleI CACnn'nnGTG

Cuts at:      0     241    4233 
   Size:        241    3992
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     181    2524    4233 
   Size:        181    2343    1709

  Fragments arranged by size: 

                2343    1709     181
 
AlwNI CAG_nnn'CTG

Cuts at:      0      19     292    1165    1317    1989    2346    2894    3355 
   Size:         19     273     873     152     672     357     548     461

Cuts at:   3355    3840    4084    4233 
   Size:        485     244     149

  Fragments arranged by size: 

                 873     672     548     485     461     357     273     244
                 152     149      19
 
ApaI G_GGCC'C

Cuts at:      0    1971    2865    2994    3978    4114    4233 
   Size:       1971     894     129     984     136     119

  Fragments arranged by size: 

                1971     984     894     136     129     119
 
ApaLI G'TGCA_C

Cuts at:      0     999    3868    4233 
   Size:        999    2869     365

  Fragments arranged by size: 

                2869     999     365
 
ApoI r'AATT_y

Cuts at:      0     646    1049    1471    3286    4052    4233 
   Size:        646     403     422    1815     766     181

  Fragments arranged by size: 

                1815     766     646     422     403     181
 
AvaI C'yCGr_G

Cuts at:      0     712    2418    2968    3241    3273    4233 
   Size:        712    1706     550     273      32     960

  Fragments arranged by size: 

                1706     960     712     550     273      32
 
BamHI G'GATC_C

Cuts at:      0     973    4233 
   Size:        973    3260
 
BanI G'GyrC_C

Cuts at:      0     185     860    2471    2515    3316    4165    4233 
   Size:        185     675    1611      44     801     849      68

  Fragments arranged by size: 

                1611     849     801     675     185      68      44
 
BanII G_rGCy'C

Cuts at:      0     409     485     719    1265    1280    1971    2030    2321 
   Size:        409      76     234     546      15     691      59     291

Cuts at:   2321    2583    2865    2994    3978    4114    4233 
   Size:        262     282     129     984     136     119

  Fragments arranged by size: 

                 984     691     546     409     291     282     262     234
                 136     129     119      76      59      15
 
BbeI G_GCGC'C

Cuts at:      0    2519    4233 
   Size:       2519    1714
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     229    1417    1889    4233 
   Size:        229    1188     472    2344

  Fragments arranged by size: 

                2344    1188     472     229
 
BbvCI CC'TCA_GC

Cuts at:      0    1682    1960    2077    2440    4233 
   Size:       1682     278     117     363    1793

  Fragments arranged by size: 

                1793    1682     363     278     117
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     141     175    2949    2983    4233 
   Size:        141      34    2774      34    1250

  Fragments arranged by size: 

                2774    1250     141      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1275    3602    4118    4233 
   Size:       1275    2327     516     115

  Fragments arranged by size: 

                2327    1275     516     115
 
BlpI GC'TnA_GC

Cuts at:      0     253    3955    4233 
   Size:        253    3702     278

  Fragments arranged by size: 

                3702     278     253
 
Bme1580I G_kGCm'C

Cuts at:      0    1003    1971    2865    2994    3872    3978    4114    4233 
   Size:       1003     968     894     129     878     106     136     119

  Fragments arranged by size: 

                1003     968     894     878     136     129     119     106
            
 
BmgBI CAC'GTC

Cuts at:      0    3294    4233 
   Size:       3294     939
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     650    1485    3737    4233 
   Size:        650     835    2252     496

  Fragments arranged by size: 

                2252     835     650     496
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2602    4233 
   Size:       2602    1631
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452    1117    1264    1553    1669    4231    4233 
   Size:        452     665     147     289     116    2562       2

  Fragments arranged by size: 

                2562     665     452     289     147     116       2
 
Bpu10I CC'TnA_GC

Cuts at:      0    1292    1682    1960    2077    2347    2440    2695    3299 
   Size:       1292     390     278     117     270      93     255     604

Cuts at:   3299    4233 
   Size:        934

  Fragments arranged by size: 

                1292     934     604     390     278     270     255     117
                  93
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1509    2239    2365    4233 
   Size:       1509     730     126    1868

  Fragments arranged by size: 

                1868    1509     730     126
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     564    2187    2457    3986    4233 
   Size:        564    1623     270    1529     247

  Fragments arranged by size: 

                1623    1529     564     270     247
 
BsaAI yAC'GTr

Cuts at:      0    1128    3873    4233 
   Size:       1128    2745     360

  Fragments arranged by size: 

                2745    1128     360
 
BsaBI GATnn'nnATC

Cuts at:      0    1074    4233 
   Size:       1074    3159
 
BsaHI Gr'CG_yC

Cuts at:      0    2248    2369    2516    4013    4233 
   Size:       2248     121     147    1497     220

  Fragments arranged by size: 

                2248    1497     220     147     121
 
BsaWI w'CCGG_w

Cuts at:      0     976    2178    2898    4233 
   Size:        976    1202     720    1335

  Fragments arranged by size: 

                1335    1202     976     720
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1240    1270    1422    1452    4204    4233 
   Size:       1240      30     152      30    2752      29

  Fragments arranged by size: 

                2752    1240     152      30      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     523    2275    3261    3907    4117    4233 
   Size:         46     477    1752     986     646     210     116

  Fragments arranged by size: 

                1752     986     646     477     210     116      46
 
BseYI C'CCAG_C

Cuts at:      0     494     896    1296    1964    2029    2336    2355    3212 
   Size:        494     402     400     668      65     307      19     857

Cuts at:   3212    3632    4233 
   Size:        420     601

  Fragments arranged by size: 

                 857     668     601     494     420     402     400     307
                  65      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547     793    1150    1580    2381    3129    3465    3508 
   Size:        547     246     357     430     801     748     336      43

Cuts at:   3508    4233 
   Size:        725

  Fragments arranged by size: 

                 801     748     725     547     430     357     336     246
                  43
 
BsiHKAI G_wGCw'C

Cuts at:      0    1003    1435    2321    3872    3961    4233 
   Size:       1003     432     886    1551      89     272

  Fragments arranged by size: 

                1551    1003     886     432     272      89
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1162    2296    4233 
   Size:       1162    1134    1937

  Fragments arranged by size: 

                1937    1162    1134
 
Bsp1286I G_dGCh'C

Cuts at:      0     409     485     719    1003    1265    1280    1435    1971 
   Size:        409      76     234     284     262      15     155     536

Cuts at:   1971    2030    2321    2583    2865    2994    3872    3961    3978 
   Size:         59     291     262     282     129     878      89      17

Cuts at:   3978    4114    4233 
   Size:        136     119

  Fragments arranged by size: 

                 878     536     409     291     284     282     262     262
                 234     155     136     129     119      89      76      59
                  17      15
 
BspEI T'CCGG_A

Cuts at:      0    2898    4233 
   Size:       2898    1335
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2547    3848    4233 
   Size:       2547    1301     385

  Fragments arranged by size: 

                2547    1301     385
 
BsrBI CCG'CTC

Cuts at:      0      95    4233 
   Size:         95    4138
 
BsrDI GCAATG_nn'

Cuts at:      0     325    4233 
   Size:        325    3908
 
BsrFI r'CCGG_y

Cuts at:      0     182    2351    4233 
   Size:        182    2169    1882

  Fragments arranged by size: 

                2169    1882     182
 
BssHII G'CGCG_C

Cuts at:      0    3513    4233 
   Size:       3513     720
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1203    1729    1897    4233 
   Size:       1203     526     168    2336

  Fragments arranged by size: 

                2336    1203     526     168
 
BstYI r'GATC_y

Cuts at:      0      77     468     973    3266    4233 
   Size:         77     391     505    2293     967

  Fragments arranged by size: 

                2293     967     505     391      77
 
Bsu36I CC'TnA_GG

Cuts at:      0    2574    4202    4233 
   Size:       2574    1628      31

  Fragments arranged by size: 

                2574    1628      31
 
BtgI C'CryG_G

Cuts at:      0     741    1190    1202    2021    2230    2277    3003    3342 
   Size:        741     449      12     819     209      47     726     339

Cuts at:   3342    3482    3599    4233 
   Size:        140     117     634

  Fragments arranged by size: 

                 819     741     726     634     449     339     209     140
                 117      47      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     126    2270    2714    4233 
   Size:        126    2144     444    1519

  Fragments arranged by size: 

                2144    1519     444     126
 
BtsI GCAGTG_nn'

Cuts at:      0    1779    2687    2917    4233 
   Size:       1779     908     230    1316

  Fragments arranged by size: 

                1779    1316     908     230
 
ClaI AT'CG_AT

Cuts at:      0       7    4233 
   Size:          7    4226
 
DraIII CAC_nnn'GTG

Cuts at:      0    3147    4233 
   Size:       3147    1086
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2247    4233 
   Size:       2247    1986
 
EaeI y'GGCC_r

Cuts at:      0    1193    1205    2070    3149    3561    4233 
   Size:       1193      12     865    1079     412     672

  Fragments arranged by size: 

                1193    1079     865     672     412      12
 
EarI CTCTTCn'nnn_

Cuts at:      0    1191    4168    4233 
   Size:       1191    2977      65

  Fragments arranged by size: 

                2977    1191      65
 
EcoICRI GAG'CTC

Cuts at:      0    2319    4233 
   Size:       2319    1914
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     614    1085    1117    1264    1553    1669    3205 
   Size:        452     162     471      32     147     289     116    1536

Cuts at:   3205    4231    4233 
   Size:       1026       2

  Fragments arranged by size: 

                1536    1026     471     452     289     162     147     116
                  32       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     294    2674    3562    4233 
   Size:        294    2380     888     671

  Fragments arranged by size: 

                2380     888     671     294
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1968    2343    2422    2733    2861    2862    2991    3438 
   Size:       1968     375      79     311     128       1     129     447

Cuts at:   3438    4206    4233 
   Size:        768      27

  Fragments arranged by size: 

                1968     768     447     375     311     129     128      79
                  27       1
 
EcoRI G'AATT_C

Cuts at:      0    3286    4233 
   Size:       3286     947
 
EcoRV GAT'ATC

Cuts at:      0      11    4233 
   Size:         11    4222
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     228    2960    4233 
   Size:        228    2732    1273

  Fragments arranged by size: 

                2732    1273     228
 
FspI TGC'GCA

Cuts at:      0    3196    4233 
   Size:       3196    1037
 
HaeII r_GCGC'y

Cuts at:      0    2519    2848    3458    3550    3955    4233 
   Size:       2519     329     610      92     405     278

  Fragments arranged by size: 

                2519     610     405     329     278      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     347     379     499     531     973    1005    1077    1109 
   Size:        347      32     120      32     442      32      72      32

Cuts at:   1109    1240    1272    1422    1454    1498    1530    2748    2780 
   Size:        131      32     150      32      44      32    1218      32

Cuts at:   2780    3717    3749    4233 
   Size:        937      32     484

  Fragments arranged by size: 

                1218     937     484     442     347     150     131     120
                  72      44      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     945    4233 
   Size:        945    3288
 
HindIII A'AGCT_T

Cuts at:      0    2942    3408    3657    4233 
   Size:       2942     466     249     576

  Fragments arranged by size: 

                2942     576     466     249
 
KasI G'GCGC_C

Cuts at:      0    2515    4233 
   Size:       2515    1718
 
MfeI C'AATT_G

Cuts at:      0     956    4233 
   Size:        956    3277
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     485     889    1914    4155    4233 
   Size:         94     391     404    1025    2241      78

  Fragments arranged by size: 

                2241    1025     404     391      94      78
 
MscI TGG'CCA

Cuts at:      0    1195    1207    2072    3151    4233 
   Size:       1195      12     865    1079    1082

  Fragments arranged by size: 

                1195    1082    1079     865      12
 
MslI CAynn'nnrTG

Cuts at:      0     145     163     241    1727    3604    4233 
   Size:        145      18      78    1486    1877     629

  Fragments arranged by size: 

                1877    1486     629     145      78      18
 
MspA1I CmG'CkG

Cuts at:      0     498     531    1253    1964    2172    2808    3257    3492 
   Size:        498      33     722     711     208     636     449     235

Cuts at:   3492    3948    3980    4233 
   Size:        456      32     253

  Fragments arranged by size: 

                 722     711     636     498     456     449     253     235
                 208      33      32
 
NaeI GCC'GGC

Cuts at:      0     184    2353    4233 
   Size:        184    2169    1880

  Fragments arranged by size: 

                2169    1880     184
 
NarI GG'CG_CC

Cuts at:      0    2516    4233 
   Size:       2516    1717
 
NcoI C'CATG_G

Cuts at:      0    1190    3003    4233 
   Size:       1190    1813    1230

  Fragments arranged by size: 

                1813    1230    1190
 
NgoMIV G'CCGG_C

Cuts at:      0     182    2351    4233 
   Size:        182    2169    1882

  Fragments arranged by size: 

                2169    1882     182
 
NspI r_CATG'y

Cuts at:      0     168    2098    3609    3668    3925    4233 
   Size:        168    1930    1511      59     257     308

  Fragments arranged by size: 

                1930    1511     308     257     168      59
 
PasI CC'CwG_GG

Cuts at:      0    1273    4233 
   Size:       1273    2960
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2559    4233 
   Size:       2559    1674
 
PfoI T'CCnGG_A

Cuts at:      0    1646    4233 
   Size:       1646    2587
 
PmlI CAC'GTG

Cuts at:      0    1128    3873    4233 
   Size:       1128    2745     360

  Fragments arranged by size: 

                2745    1128     360
 
PpuMI rG'GwC_Cy

Cuts at:      0    2343    2422    2733    3438    4206    4233 
   Size:       2343      79     311     705     768      27

  Fragments arranged by size: 

                2343     768     705     311      79      27
 
PshAI GACnn'nnGTC

Cuts at:      0    2117    2179    4233 
   Size:       2117      62    2054

  Fragments arranged by size: 

                2117    2054      62
 
PsiI TTA'TAA

Cuts at:      0    1045    4233 
   Size:       1045    3188
 
PspOMI G'GGCC_C

Cuts at:      0    1967    2861    2990    3974    4110    4233 
   Size:       1967     894     129     984     136     123

  Fragments arranged by size: 

                1967     984     894     136     129     123
 
PstI C_TGCA'G

Cuts at:      0    1812    2503    3390    4233 
   Size:       1812     691     887     843

  Fragments arranged by size: 

                1812     887     843     691
 
PvuII CAG'CTG

Cuts at:      0     498     531    1964    2172    3492    3948    4233 
   Size:        498      33    1433     208    1320     456     285

  Fragments arranged by size: 

                1433    1320     498     456     285     208      33
 
RsrII CG'GwC_CG

Cuts at:      0    3878    4233 
   Size:       3878     355
 
SacI G_AGCT'C

Cuts at:      0    2321    4233 
   Size:       2321    1912
 
SanDI GG'GwC_CC

Cuts at:      0    2422    4233 
   Size:       2422    1811
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1191    4168    4233 
   Size:       1191    2977      65

  Fragments arranged by size: 

                2977    1191      65
 
ScaI AGT'ACT

Cuts at:      0     639    4233 
   Size:        639    3594
 
SexAI A'CCwGG_T

Cuts at:      0     269    4233 
   Size:        269    3964
 
SfcI C'TryA_G

Cuts at:      0    1808    2499    3386    4233 
   Size:       1808     691     887     847

  Fragments arranged by size: 

                1808     887     847     691
 
SfoI GGC'GCC

Cuts at:      0    2517    4233 
   Size:       2517    1716
 
SmaI CCC'GGG

Cuts at:      0     714    4233 
   Size:        714    3519
 
SmlI C'TyrA_G

Cuts at:      0    1524    2218    2380    4233 
   Size:       1524     694     162    1853

  Fragments arranged by size: 

                1853    1524     694     162
 
SphI G_CATG'C

Cuts at:      0     168    2098    3609    4233 
   Size:        168    1930    1511     624

  Fragments arranged by size: 

                1930    1511     624     168
 
SrfI GCCC'GGGC

Cuts at:      0     714    4233 
   Size:        714    3519
 
SspI AAT'ATT

Cuts at:      0    1016    4233 
   Size:       1016    3217
 
StuI AGG'CCT

Cuts at:      0    1062    2065    3940    4233 
   Size:       1062    1003    1875     293

  Fragments arranged by size: 

                1875    1062    1003     293
 
StyI C'CwwG_G

Cuts at:      0     628    1190    1216    2189    3003    3048    4233 
   Size:        628     562      26     973     814      45    1185

  Fragments arranged by size: 

                1185     973     814     628     562      45      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     888    2347    3834    4183    4233 
   Size:        888    1459    1487     349      50

  Fragments arranged by size: 

                1487    1459     888     349      50
 
TatI w'GTAC_w

Cuts at:      0      47     176     206     637    4233 
   Size:         47     129      30     431    3596

  Fragments arranged by size: 

                3596     431     129      47      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     636     668    3526    3558    4204    4233 
   Size:        636      32    2858      32     646      29

  Fragments arranged by size: 

                2858     646     636      32      32      29
 
Tth111I GACn'n_nGTC

Cuts at:      0     940    2926    4086    4233 
   Size:        940    1986    1160     147

  Fragments arranged by size: 

                1986    1160     940     147
 
XbaI T'CTAG_A

Cuts at:      0     471    4233 
   Size:        471    3762
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     528    1293    3144    4233 
   Size:        528     765    1851    1089

  Fragments arranged by size: 

                1851    1089     765     528
 
XmaI C'CCGG_G

Cuts at:      0     712    4233 
   Size:        712    3521
 
XmnI GAAnn'nnTTC

Cuts at:      0      88     424    3286    4233 
   Size:         88     336    2862     947

  Fragments arranged by size: 

                2862     947     336      88
 
ZraI GAC'GTC

Cuts at:      0    2249    4233 
   Size:       2249    1984
 

 Enzymes that do cut:

     AarI    AatII     AclI     AcuI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI     PstI    PvuII    RsrII     SacI    SanDI     SapI     ScaI
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     SrfI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AfeI    AflII     AgeI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI    BglII     BmtI
    BsiEI    BsiWI     BsmI    BspHI    BsrGI    BssSI   BstAPI    BstBI
   BstEII  BstZ17I    CspCI     DraI     EagI     EciI     FseI    FspAI
     HpaI     KpnI     MluI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PpiI    PspXI     PsrI     PvuI    SacII
     SalI     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SwaI
     XhoI
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