Restriction map for pF1KA0321
##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AclI        AgeI        Alw44I      ApaLI       
AscI        AseI        AsnI        AspI        AviII       
AvrII       BbeI        BlnI        BsiEI       BsiWI       
Bsp1407I    BspDI       BspLU11I    BsrGI       BssHII      
ClaI        CpoI        DraI        EagI        EclXI       
Eco52I      FseI        FspI        HpaI        KasI        
KspI        MfeI        MluI        MunI        NarI        
NdeI        NotI        NruI        PacI        PflFI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SfiI        SfoI        SgrAI       
SnaBI       SspBI       SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I       5427  GGTACC               G^GTACC
AccII        2527  CGCG                 CG^CG
AccIII        293  TCCGGA               T^CCGGA
AcyI         5492  GGCGTC               G[GA]^CG[CT]C
AflIII       2281  ACACGT               A^C[GA][CT]GT
Asp718I      5427  GGTACC               G^GTACC
BamHI        4966  GGATCC               G^GATCC
BcgI         6378  CGATTACTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        6361  GTATCC               GTATCC(6/5)
BsaBI        5282  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        5492  GGCGTC               G[GA]^CG[CT]C
BseAI         293  TCCGGA               T^CCGGA
BsmBI         442  CGTCTC               CGTCTC(1/5)
BspEI         293  TCCGGA               T^CCGGA
BssSI        5582  CTCGTG               CACGAG(-5/-1)
Bst1107I     5330  GTATAC               GTA^TAC
BstBI         590  TTCGAA               TT^CGAA
BstEII       5851  GGTCACC              G^GT[ACGT]ACC
BstPI        5851  GGTCACC              G^GT[ACGT]ACC
BstUI        2527  CGCG                 CG^CG
BstZ17I      5330  GTATAC               GTA^TAC
DrdI         4498  GACACGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      5851  GGTCACC              G^GT[ACGT]ACC
EcoRV         133  GATATC               GAT^ATC
EcoT22I      4409  ATGCAT               ATGCA^T
Hin1I        5492  GGCGTC               G[GA]^CG[CT]C
HindIII       301  AAGCTT               A^AGCTT
KpnI         5427  GGTACC               GGTAC^C
MamI         5282  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          293  TCCGGA               T^CCGGA
MvnI         2527  CGCG                 CG^CG
NheI          921  GCTAGC               G^CTAGC
NsiI         4409  ATGCAT               ATGCA^T
NspV          590  TTCGAA               TT^CGAA
SalI         3020  GTCGAC               G^TCGAC
SbfI          737  CCTGCAGG             CCTGCA^GG
SexAI        7021  ACCAGGT              A^CC[AT]GGT
SfuI          590  TTCGAA               TT^CGAA
SpeI         2014  ACTAGT               A^CTAGT
SphI         6198  GCATGC               GCATG^C
Sse8387I      737  CCTGCAGG             CCTGCA^GG
SspI         6037  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         3020  GTCGAC               GT^[AC][GT]AC
             5330  GTATAC              
AflII        3922  CTTAAG               C^TTAAG
             6877  CTTAAG              
AhdI          435  GACTCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3117  GACAGCCAGTC         
Aor51HI      2612  AGCGCT               AGC^GCT
             2740  AGCGCT              
AspEI         435  GACTCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3117  GACAGCCAGTC         
BbrPI         400  CACGTG               CAC^GTG
             6064  CACGTG              
BclI         3522  TGATCA               T^GATCA
             5592  TGATCA              
BfrI         3922  CTTAAG               C^TTAAG
             6877  CTTAAG              
BlpI          777  GCTCAGC              GC^T[ACGT]AGC
             2382  GCTGAGC             
Bpu1102I      777  GCTCAGC              GC^T[ACGT]AGC
             2382  GCTGAGC             
BsrFI        5516  GCCGGC               [GA]^CCGG[CT]
             5977  GCCGGC              
BstXI        2325  CCAGGCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4908  CCACTTCTATGG        
CelII         777  GCTCAGC              GC^T[ACGT]AGC
             2382  GCTGAGC             
Cfr10I       5516  GCCGGC               [GA]^CCGG[CT]
             5977  GCCGGC              
Eam1105I      435  GACTCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3117  GACAGCCAGTC         
Eco47III     2612  AGCGCT               AGC^GCT
             2740  AGCGCT              
EcoRI        1081  GAATTC               G^AATTC
             7358  GAATTC              
FbaI         3522  TGATCA               T^GATCA
             5592  TGATCA              
HgaI         6420  GACGC                GACGC(5/10)
             5493  GCGTC               
HincII       1676  GTCAAC               GT[CT]^[GA]AC
             3020  GTCGAC              
HindII       1676  GTCAAC               GT[CT]^[GA]AC
             3020  GTCGAC              
NaeI         5516  GCCGGC               GCC^GGC
             5977  GCCGGC              
NcoI         2912  CCATGG               C^CATGG
             7596  CCATGG              
NgoMIV       5516  GCCGGC               G^CCGGC
             5977  GCCGGC              
PaeR7I        358  CTCGAG               C^TCGAG
             1003  CTCGAG              
PmaCI         400  CACGTG               CAC^GTG
             6064  CACGTG              
PmlI          400  CACGTG               CAC^GTG
             6064  CACGTG              
PshAI        2896  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4926  GACTGCTGTC          
ScaI         6137  AGTACT               AGT^ACT
             6707  AGTACT              
StuI          734  AGGCCT               AGG^CCT
             4100  AGGCCT              
XhoI          358  CTCGAG               C^TCGAG
             1003  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BglII       BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspHI       BspMI       BsrBI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XcmI        XhoII       XmaCI       XmaI        
XmnI        
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