Restriction map for pF1KA0303
##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AatII       AflII       AhdI        AscI        AspEI       
AspI        AvrII       BbrPI       BclI        BfrI        
BlnI        BsaAI       BsiWI       BspLU11I    Bst1107I    
BstZ17I     DraI        DrdI        Eam1105I    EcoT22I     
FbaI        HincII      HindII      HpaI        MfeI        
MluI        MunI        NotI        NruI        NsiI        
PacI        PflFI       PmaCI       PmeI        PmlI        
PshAI       SacI        SalI        SbfI        SexAI       
SfiI        SgrAI       SnaBI       Sse8387I    SwaI        
Tth111I     


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       3302  GGTACC               G^GTACC
AclI          622  AACGTT               AA^CGTT
AflIII       3692  ACGTGT               A^C[GA][CT]GT
Alw44I       2725  GTGCAC               G^TGCAC
ApaLI        2725  GTGCAC               G^TGCAC
AseI         1219  ATTAAT               AT^TAAT
AsnI         1219  ATTAAT               AT^TAAT
Asp718I      3302  GGTACC               G^GTACC
AviII        5769  TGCGCA               TGC^GCA
BamHI         996  GGATCC               G^GATCC
BcgI         5166  CGAGCGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1773  GGATAC               GTATCC(6/5)
BsaBI        2640  GATTGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         4363  GAATGC               GAATGC(1/-1)
Bsp1407I     1485  TGTACA               T^GTACA
BspHI         777  TCATGA               T^CATGA
BsrGI        1485  TGTACA               T^GTACA
BssHII       4056  GCGCGC               G^CGCGC
BssSI        2582  CACGAG               CACGAG(-5/-1)
CpoI         3307  CGGTCCG              CG^G[AT]CCG
EagI         6299  CGGCCG               C^GGCCG
EclXI        6299  CGGCCG               C^GGCCG
Eco52I       6299  CGGCCG               C^GGCCG
EcoRV        1276  GATATC               GAT^ATC
FseI         6381  GGCCGGCC             GGCCGG^CC
FspI         5769  TGCGCA               TGC^GCA
KpnI         3302  GGTACC               GGTAC^C
KspI         6437  CCGCGG               CCGC^GG
MamI         2640  GATTGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI         3024  GCTAGC               G^CTAGC
PshBI        1219  ATTAAT               AT^TAAT
Psp1406I      622  AACGTT               AA^CGTT
PvuI         4616  CGATCG               CGAT^CG
RcaI          777  TCATGA               T^CATGA
RsrII        3307  CGGTCCG              CG^G[AT]CCG
SacII        6437  CCGCGG               CCGC^GG
ScaI         3970  AGTACT               AGT^ACT
SpeI          819  ACTAGT               A^CTAGT
SspBI        1485  TGTACA               T^GTACA
XbaI         6060  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          541  GTCTAC               GT^[AC][GT]AC
             1168  GTCTAC              
Aor51HI      5168  AGCGCT               AGC^GCT
             5469  AGCGCT              
BbsI         2092  GAAGAC               GAAGAC(2/6)
             2197  GAAGAC              
BglI         1156  GCCTATGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4106  GCCAGGGTGGC         
BglII        2790  AGATCT               A^GATCT
             3007  AGATCT              
BlpI         3711  GCTCAGC              GC^T[ACGT]AGC
             6730  GCTCAGC             
Bpu1102I     3711  GCTCAGC              GC^T[ACGT]AGC
             6730  GCTCAGC             
BpuAI        2092  GAAGAC               GAAGAC(2/6)
             2197  GAAGAC              
BspDI        1937  ATCGAT               AT^CGAT
             4273  ATCGAT              
BspMI        4826  ACCTGC               ACCTGC(4/8)
             5032  ACCTGC              
CelII        3711  GCTCAGC              GC^T[ACGT]AGC
             6730  GCTCAGC             
ClaI         1937  ATCGAT               AT^CGAT
             4273  ATCGAT              
DraIII       3493  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6246  CACCCTGTG           
Eco47III     5168  AGCGCT               AGC^GCT
             5469  AGCGCT              
EcoNI        1593  CCTTTACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4020  CCTGCTGAAGG         
NdeI         1919  CATATG               CA^TATG
             2056  CATATG              
PvuII        4349  CAGCTG               CAG^CTG
             5405  CAGCTG              
SapI         4563  GAAGAGC              GCTCTTC(1/4)
             4033  GCTCTTC             
SmaI         3886  CCCGGG               CCC^GGG
             5814  CCCGGG              
SphI         2764  GCATGC               GCATG^C
             6686  GCATGC              
SspI         1463  AATATT               AAT^ATT
             2264  AATATT              
XmaCI        3886  CCCGGG               C^CCGGG
             5814  CCCGGG              
XmaI         3886  CCCGGG               C^CCGGG
             5814  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  149 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AgeI        AluI        AlwI        AlwNI       
AocI        ApaI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvCI       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspEI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        NspV        PaeR7I      
PflMI       PinAI       PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SfuI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoI        XhoII       XmnI        
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