Restriction map for
pF1KA0303
(Circular) (Six-base) MAPSORT of: pF1KA0303.seq Check: 7168 from: 1 to: 10199
pF1KA0303 10199 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 650 1277 7237 650
Size: 627 5960 3612
Fragments arranged by size:
5960 3612 627
Acc65I G'GTAC_C
Cuts at: 3410 7215 3410
Size: 3805 6394
AclI AA'CG_TT
Cuts at: 731 10039 731
Size: 9308 891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2570 2908 2989 3940 4153 4215 4462 4738
Size: 177 338 81 951 213 62 247 276
Cuts at: 4738 5222 6470 8024 8456 8809 9471 2393
Size: 484 1248 1554 432 353 662 3121
Fragments arranged by size:
3121 1554 1248 951 662 484 432 353
338 276 247 213 177 81 62
AfeI AGC'GCT
Cuts at: 5278 5579 5278
Size: 301 9898
AflIII A'CryG_T
Cuts at: 3800 7551 9351 9632 3800
Size: 3751 1800 281 4367
Fragments arranged by size:
4367 3751 1800 281
AgeI A'CCGG_T
Cuts at: 3413 5951 6518 3413
Size: 2538 567 7094
Fragments arranged by size:
7094 2538 567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1328 2353 1328
Size: 1025 9174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4933 4965 4933
Size: 32 10167
AlwNI CAG_nnn'CTG
Cuts at: 1971 2416 2728 3789 4798 5338 6282 6655 7504
Size: 445 312 1061 1009 540 944 373 849
Cuts at: 7504 8942 1971
Size: 1438 3228
Fragments arranged by size:
3228 1438 1061 1009 944 849 540 445
373 312
ApaI G_GGCC'C
Cuts at: 4242 5929 6137 6590 4242
Size: 1687 208 453 7851
Fragments arranged by size:
7851 1687 453 208
ApaLI G'TGCA_C
Cuts at: 2833 9037 2833
Size: 6204 3995
ApoI r'AATT_y
Cuts at: 315 985 1000 1849 2095 2286 4977 5519 7203
Size: 670 15 849 246 191 2691 542 1684
Cuts at: 7203 9552 9808 315
Size: 2349 256 706
Fragments arranged by size:
2691 2349 1684 849 706 670 542 256
246 191 15
AseI AT'TA_AT
Cuts at: 20 1328 20
Size: 1308 8891
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10199
AvaI C'yCGr_G
Cuts at: 791 1220 3994 5844 5922 6396 6484 7219 791
Size: 429 2774 1850 78 474 88 735 3771
Fragments arranged by size:
3771 2774 1850 735 474 429 88 78
BamHI G'GATC_C
Cuts at: 1104 7224 1104
Size: 6120 4079
BanI G'GyrC_C
Cuts at: 1445 1947 3410 3774 4110 4197 4252 5433 5668
Size: 502 1463 364 336 87 55 1181 235
Cuts at: 5668 7215 7629 7877 7912 1445
Size: 1547 414 248 35 3732
Fragments arranged by size:
3732 1547 1463 1181 502 414 364 336
248 235 87 55 35
BanII G_rGCy'C
Cuts at: 598 3629 4242 4520 4678 4810 5053 5312 5840
Size: 3031 613 278 158 132 243 259 528
Cuts at: 5840 5851 5929 6137 6165 6590 6975 7213 8243
Size: 11 78 208 28 425 385 238 1030
Cuts at: 8243 598
Size: 2554
Fragments arranged by size:
3031 2554 1030 613 528 425 385 278
259 243 238 208 158 132 78 28
11
BbeI G_GCGC'C
Cuts at: 1449 3778 4114 5437 7633 7881 1449
Size: 2329 336 1323 2196 248 3767
Fragments arranged by size:
3767 2329 2196 1323 336 248
BbsI GAAGACnn'nnnn_
Cuts at: 2207 2312 2207
Size: 105 10094
BbvCI CC'TCA_GC
Cuts at: 333 2718 6648 333
Size: 2385 3930 3884
Fragments arranged by size:
3930 3884 2385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5263 5297 5263
Size: 34 10165
BciVI GTATCCnnnnn_n'
Cuts at: 1875 8097 9153 10199 1875
Size: 6222 1056 1046 1875
Fragments arranged by size:
6222 1875 1056 1046
BclI T'GATC_A
Cuts at: 7719 7719
Size: 10199
BglI GCCn_nnn'nGGC
Cuts at: 1270 4220 1270
Size: 2950 7249
BglII A'GATC_T
Cuts at: 2898 3115 7714 9751 2898
Size: 217 4599 2037 3346
Fragments arranged by size:
4599 3346 2037 217
BlpI GC'TnA_GC
Cuts at: 3820 6839 7311 3820
Size: 3019 472 6708
Fragments arranged by size:
6708 3019 472
Bme1580I G_kGCm'C
Cuts at: 1643 2562 2837 3333 3359 4242 4735 5929 6137
Size: 919 275 496 26 883 493 1194 208
Cuts at: 6137 6590 7824 7917 9041 1643
Size: 453 1234 93 1124 2801
Fragments arranged by size:
2801 1234 1194 1124 919 883 496 493
453 275 208 93 26
BmgBI CAC'GTC
Cuts at: 3810 4827 7068 3810
Size: 1017 2241 6941
Fragments arranged by size:
6941 2241 1017
BmrI ACTGGGnnnn_n'
Cuts at: 320 1099 3544 3856 4483 6665 7411 7492 7827
Size: 779 2445 312 627 2182 746 81 335
Cuts at: 7827 9971 320
Size: 2144 548
Fragments arranged by size:
2445 2182 2144 779 746 627 548 335
312 81
BmtI G_CTAG'C
Cuts at: 3136 3136
Size: 10199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 4341 5112 5859 8566 3134
Size: 1207 771 747 2707 4767
Fragments arranged by size:
4767 2707 1207 771 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2023 2382 2528 2915 3748 4243 4957 4960
Size: 152 359 146 387 833 495 714 3
Cuts at: 4960 5625 6229 6238 6449 6696 6820 6937 1871
Size: 665 604 9 211 247 124 117 5133
Fragments arranged by size:
5133 833 714 665 604 495 387 359
247 211 152 146 124 117 9 3
Bpu10I CC'TnA_GC
Cuts at: 333 741 2718 6648 333
Size: 408 1977 3930 3884
Fragments arranged by size:
3930 3884 1977 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 3631 4429 4582 5199 7375 8254 8968 9266
Size: 3347 798 153 617 2176 879 714 298
Cuts at: 9266 284
Size: 1217
Fragments arranged by size:
3347 2176 1217 879 798 714 617 298
153
BsaI GGTCTCn'nnnn_
Cuts at: 438 2504 3254 4273 4975 5600 6906 9903 438
Size: 2066 750 1019 702 625 1306 2997 734
Fragments arranged by size:
2997 2066 1306 1019 750 734 702 625
BsaAI yAC'GTr
Cuts at: 7552 7552
Size: 10199
BsaBI GATnn'nnATC
Cuts at: 6 2752 7713 9750 6
Size: 2746 4961 2037 455
Fragments arranged by size:
4961 2746 2037 455
BsaHI Gr'CG_yC
Cuts at: 369 1446 3469 3775 4111 5434 6173 7630 7878
Size: 1077 2023 306 336 1323 739 1457 248
Cuts at: 7878 8580 10076 369
Size: 702 1496 492
Fragments arranged by size:
2023 1496 1457 1323 1077 739 702 492
336 306 248
BsaWI w'CCGG_w
Cuts at: 215 993 3413 3418 4756 5951 6518 7388 7909
Size: 778 2420 5 1338 1195 567 870 521
Cuts at: 7909 8587 8998 9145 9419 215
Size: 678 411 147 274 995
Fragments arranged by size:
2420 1338 1195 995 870 778 678 567
521 411 274 147 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2912 2942 3459 3489 3658 3688 3694 3724 4835
Size: 30 517 30 169 30 6 30 1111
Cuts at: 4835 4865 4933 4963 6297 6327 2912
Size: 30 68 30 1334 30 6784
Fragments arranged by size:
6784 1334 1111 517 169 68 30 30
30 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 1906 1975 2536 2581 3126 3679 3757 5359
Size: 1723 69 561 45 545 553 78 1602
Cuts at: 5359 5490 5952 6155 6699 183
Size: 131 462 203 544 3683
Fragments arranged by size:
3683 1723 1602 561 553 545 544 462
203 131 78 69 45
BseYI C'CCAG_C
Cuts at: 421 2010 2586 2704 3342 3367 3565 3764 4119
Size: 1589 576 118 638 25 198 199 355
Cuts at: 4119 4810 4855 4960 5538 5713 5824 5839 6814
Size: 691 45 105 578 175 111 15 975
Cuts at: 6814 7023 7028 7038 7083 7624 8382 9047 9455
Size: 209 5 10 45 541 758 665 408
Cuts at: 9455 421
Size: 1165
Fragments arranged by size:
1589 1165 975 758 691 665 638 578
576 541 408 355 209 199 198 175
118 111 105 45 45 25 15 10
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 3769 4784 5491 6133 3676
Size: 93 1015 707 642 7742
Fragments arranged by size:
7742 1015 707 642 93
BsiEI CG_ry'CG
Cuts at: 104 4727 5952 6410 7741 7787 9017 104
Size: 4623 1225 458 1331 46 1230 1286
Fragments arranged by size:
4623 1331 1286 1230 1225 458 46
BsiHKAI G_wGCw'C
Cuts at: 143 2837 3712 4211 7213 7991 8181 9041 143
Size: 2694 875 499 3002 778 190 860 1301
Fragments arranged by size:
3002 2694 1301 875 860 778 499 190
BsmI GAATG_Cn'
Cuts at: 4477 4477
Size: 10199
BsmBI CGTCTCn'nnnn_
Cuts at: 1546 4587 7058 1546
Size: 3041 2471 4687
Fragments arranged by size:
4687 3041 2471
Bsp1286I G_dGCh'C
Cuts at: 143 598 1643 2562 2837 3333 3359 3629 3712
Size: 455 1045 919 275 496 26 270 83
Cuts at: 3712 4211 4242 4520 4678 4735 4810 5053 5312
Size: 499 31 278 158 57 75 243 259
Cuts at: 5312 5840 5851 5929 6137 6165 6590 6975 7213
Size: 528 11 78 208 28 425 385 238
Cuts at: 7213 7824 7917 7991 8181 8243 9041 143
Size: 611 93 74 190 62 798 1301
Fragments arranged by size:
1301 1045 919 798 611 528 499 496
455 425 385 278 275 270 259 243
238 208 190 158 93 83 78 75
74 62 57 31 28 26 11
BspEI T'CCGG_A
Cuts at: 993 3418 4756 993
Size: 2425 1338 6436
Fragments arranged by size:
6436 2425 1338
BspHI T'CATG_A
Cuts at: 885 8631 885
Size: 7746 2453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4943 5149 7249 7463 7765 8146 4943
Size: 206 2100 214 302 381 6996
Fragments arranged by size:
6996 2100 381 302 214 206
BsrBI CCG'CTC
Cuts at: 50 1380 3269 3819 4246 5193 6537 8491 8545
Size: 1330 1889 550 427 947 1344 1954 54
Cuts at: 8545 10029 10194 50
Size: 1484 165 55
Fragments arranged by size:
1954 1889 1484 1344 1330 947 550 427
165 55 54
BsrDI GCAATG_nn'
Cuts at: 240 250 1263 1772 4742 5775 8111 240
Size: 10 1013 509 2970 1033 2336 2328
Fragments arranged by size:
2970 2336 2328 1033 1013 509 10
BsrFI r'CCGG_y
Cuts at: 1812 3413 3436 4524 5951 6405 6490 6518 8197
Size: 1601 23 1088 1427 454 85 28 1679
Cuts at: 8197 8378 1812
Size: 181 3633
Fragments arranged by size:
3633 1679 1601 1427 1088 454 181 85
28 23
BsrGI T'GTAC_A
Cuts at: 1593 1593
Size: 10199
BssHII G'CGCG_C
Cuts at: 4164 4164
Size: 10199
BssSI C'ACGA_G
Cuts at: 2690 8470 9178 2690
Size: 5780 708 3711
Fragments arranged by size:
5780 3711 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1427 2728 6460 7576 1427
Size: 1301 3732 1116 4050
Fragments arranged by size:
4050 3732 1301 1116
BstBI TT'CG_AA
Cuts at: 933 1209 1350 8560 933
Size: 276 141 7210 2572
Fragments arranged by size:
7210 2572 276 141
BstEII G'GTnAC_C
Cuts at: 1626 2577 3506 3732 1626
Size: 951 929 226 8093
Fragments arranged by size:
8093 951 929 226
BstXI CCAn_nnnn'nTGG
Cuts at: 3933 5461 5800 3933
Size: 1528 339 8332
Fragments arranged by size:
8332 1528 339
BstYI r'GATC_y
Cuts at: 1 938 1104 1358 1968 2898 3115 3190 7224
Size: 937 166 254 610 930 217 75 4034
Cuts at: 7224 7691 7714 8049 8295 8601 8613 8699 9751
Size: 467 23 335 246 306 12 86 1052
Cuts at: 9751 1
Size: 449
Fragments arranged by size:
4034 1052 937 930 610 467 449 335
306 254 246 217 166 86 75 23
12
Bsu36I CC'TnA_GG
Cuts at: 1987 2573 4763 5233 6569 1987
Size: 586 2190 470 1336 5617
Fragments arranged by size:
5617 2190 1336 586 470
BtgI C'CryG_G
Cuts at: 107 1634 1829 3378 3987 4499 5315 5417 6545
Size: 1527 195 1549 609 512 816 102 1128
Cuts at: 6545 6677 6944 107
Size: 132 267 3362
Fragments arranged by size:
3362 1549 1527 1128 816 609 512 267
195 132 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 522 4195 6122 8149 8330 8493 522
Size: 3673 1927 2027 181 163 2228
Fragments arranged by size:
3673 2228 2027 1927 181 163
BtsI GCAGTG_nn'
Cuts at: 3696 4869 5828 6145 7442 3696
Size: 1173 959 317 1297 6453
Fragments arranged by size:
6453 1297 1173 959 317
ClaI AT'CG_AT
Cuts at: 2046 4382 2046
Size: 2336 7863
DraI TTT'AAA
Cuts at: 7198 7198
Size: 10199
DraIII CAC_nnn'GTG
Cuts at: 3606 6359 3606
Size: 2753 7446
DrdI GACnn_nn'nnGTC
Cuts at: 7905 9249 7905
Size: 1344 8855
EaeI y'GGCC_r
Cuts at: 1532 3832 4373 5174 5741 6117 6407 6538 7784
Size: 2300 541 801 567 376 290 131 1246
Cuts at: 7784 7958 8349 8376 9721 1532
Size: 174 391 27 1345 2010
Fragments arranged by size:
2300 2010 1345 1246 801 567 541 391
376 290 174 131 27
EagI C'GGCC_G
Cuts at: 6407 7784 6407
Size: 1377 8822
EarI CTCTTCn'nnn_
Cuts at: 441 2291 2412 2546 2965 3524 4028 4148 4666
Size: 1850 121 134 419 559 504 120 518
Cuts at: 4666 5620 6777 8222 8432 441
Size: 954 1157 1445 210 2208
Fragments arranged by size:
2208 1850 1445 1157 954 559 518 504
419 210 134 121 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3087 3423 5717 6445 8407 9149 9295 10009 3087
Size: 336 2294 728 1962 742 146 714 3277
Fragments arranged by size:
3277 2294 1962 742 728 714 336 146
EcoICRI GAG'CTC
Cuts at: 7211 7211
Size: 10199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2023 2382 2393 2528 2570 2908 2915 2989
Size: 152 359 11 135 42 338 7 74
Cuts at: 2989 3748 3940 4153 4215 4243 4462 4738 4957
Size: 759 192 213 62 28 219 276 219
Cuts at: 4957 4960 5222 5625 6229 6238 6449 6470 6696
Size: 3 262 403 604 9 211 21 226
Cuts at: 6696 6820 6937 8024 8456 8809 9471 1871
Size: 124 117 1087 432 353 662 2599
Fragments arranged by size:
2599 1087 759 662 604 432 403 359
353 338 276 262 226 219 219 213
211 192 152 135 124 117 74 62
42 28 21 11 9 7 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1705 4132 1705
Size: 2427 7772
EcoO109I rG'GnC_Cy
Cuts at: 819 1513 3635 4239 4832 5029 5254 5384 5480
Size: 694 2122 604 593 197 225 130 96
Cuts at: 5480 5856 5926 6134 6256 6586 7014 7338 819
Size: 376 70 208 122 330 428 324 3680
Fragments arranged by size:
3680 2122 694 604 593 428 376 330
324 225 208 197 130 122 96 70
EcoRI G'AATT_C
Cuts at: 2286 4977 5519 7203 2286
Size: 2691 542 1684 5282
Fragments arranged by size:
5282 2691 1684 542
EcoRV GAT'ATC
Cuts at: 1386 1386
Size: 10199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1125 4659 5599 5813 8218 1125
Size: 3534 940 214 2405 3106
Fragments arranged by size:
3534 3106 2405 940 214
FseI GG_CCGG'CC
Cuts at: 6494 6494
Size: 10199
FspI TGC'GCA
Cuts at: 5879 7980 5879
Size: 2101 8098
HaeII r_GCGC'y
Cuts at: 1449 3778 4114 5280 5437 5581 7633 7881 9111
Size: 2329 336 1166 157 144 2052 248 1230
Cuts at: 9111 9882 1449
Size: 771 1766
Fragments arranged by size:
2329 2052 1766 1230 1166 771 336 248
157 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1895 1927 2131 2163 2420 2452 2888 2920 3271
Size: 32 204 32 257 32 436 32 351
Cuts at: 3271 3303 3459 3491 3658 3690 4532 4564 5485
Size: 32 156 32 167 32 842 32 921
Cuts at: 5485 5517 8041 8071 8073 8103 1895
Size: 32 2524 30 2 30 3991
Fragments arranged by size:
3991 2524 921 842 436 351 257 204
167 156 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 7238 7238
Size: 10199
HindIII A'AGCT_T
Cuts at: 222 5131 5204 222
Size: 4909 73 5217
Fragments arranged by size:
5217 4909 73
KasI G'GCGC_C
Cuts at: 1445 3774 4110 5433 7629 7877 1445
Size: 2329 336 1323 2196 248 3767
Fragments arranged by size:
3767 2329 2196 1323 336 248
KpnI G_GTAC'C
Cuts at: 3414 7219 3414
Size: 3805 6394
MluI A'CGCG_T
Cuts at: 9632 9632
Size: 10199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1839 2426 3277 3502 4166 4518 8958 9142 1839
Size: 587 851 225 664 352 4440 184 2896
Fragments arranged by size:
4440 2896 851 664 587 352 225 184
MscI TGG'CCA
Cuts at: 1534 4375 5176 6119 7960 1534
Size: 2841 801 943 1841 3773
Fragments arranged by size:
3773 2841 1841 943 801
MslI CAynn'nnrTG
Cuts at: 112 568 1462 1583 2800 2915 5459 8188 8315
Size: 456 894 121 1217 115 2544 2729 127
Cuts at: 8315 112
Size: 1996
Fragments arranged by size:
2729 2544 1996 1217 894 456 127 121
115
MspA1I CmG'CkG
Cuts at: 374 3408 3459 3504 3642 3753 3768 3780 4119
Size: 3034 51 45 138 111 15 12 339
Cuts at: 4119 4459 5515 6271 6289 6547 7310 7624 7984
Size: 340 1056 756 18 258 763 314 360
Cuts at: 7984 8768 9013 374
Size: 784 245 1560
Fragments arranged by size:
3034 1560 1056 784 763 756 360 340
339 314 258 245 138 111 51 45
18 15 12
NaeI GCC'GGC
Cuts at: 3438 4526 6407 6492 8380 3438
Size: 1088 1881 85 1888 5257
Fragments arranged by size:
5257 1888 1881 1088 85
NarI GG'CG_CC
Cuts at: 1446 3775 4111 5434 7630 7878 1446
Size: 2329 336 1323 2196 248 3767
Fragments arranged by size:
3767 2329 2196 1323 336 248
NcoI C'CATG_G
Cuts at: 107 1634 1829 3987 5315 5417 6944 107
Size: 1527 195 2158 1328 102 1527 3362
Fragments arranged by size:
3362 2158 1527 1527 1328 195 102
NdeI CA'TA_TG
Cuts at: 2028 2165 2028
Size: 137 10062
NgoMIV G'CCGG_C
Cuts at: 3436 4524 6405 6490 8378 3436
Size: 1088 1881 85 1888 5257
Fragments arranged by size:
5257 1888 1881 1088 85
NheI G'CTAG_C
Cuts at: 3132 3132
Size: 10199
NspI r_CATG'y
Cuts at: 1425 2876 5303 6798 7252 7752 9355 1425
Size: 1451 2427 1495 454 500 1603 2269
Fragments arranged by size:
2427 2269 1603 1495 1451 500 454
PasI CC'CwG_GG
Cuts at: 6253 6253
Size: 10199
PciI A'CATG_T
Cuts at: 9351 9351
Size: 10199
PflMI CCAn_nnn'nTGG
Cuts at: 131 1876 2932 5418 131
Size: 1745 1056 2486 4912
Fragments arranged by size:
4912 2486 1745 1056
PfoI T'CCnGG_A
Cuts at: 1739 3868 4259 6686 1739
Size: 2129 391 2427 5252
Fragments arranged by size:
5252 2427 2129 391
PmeI GTTT'AAAC
Cuts at: 7198 7198
Size: 10199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2349 2381 4933 4965 8645 8677 2349
Size: 32 2552 32 3680 32 3871
Fragments arranged by size:
3871 3680 2552 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1513 3635 5029 5384 5856 7014 1513
Size: 2122 1394 355 472 1158 4698
Fragments arranged by size:
4698 2122 1394 1158 472 355
PspOMI G'GGCC_C
Cuts at: 4238 5925 6133 6586 4238
Size: 1687 208 453 7851
Fragments arranged by size:
7851 1687 453 208
PspXI vC'TCGA_Gb
Cuts at: 5844 6484 5844
Size: 640 9559
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 634 666 634
Size: 32 10167
PstI C_TGCA'G
Cuts at: 3785 5603 5835 6756 7246 7931 3785
Size: 1818 232 921 490 685 6053
Fragments arranged by size:
6053 1818 921 685 490 232
PvuI CG_AT'CG
Cuts at: 104 4727 104
Size: 4623 5576
PvuII CAG'CTG
Cuts at: 4459 5515 7624 7984 4459
Size: 1056 2109 360 6674
Fragments arranged by size:
6674 2109 1056 360
RsrII CG'GwC_CG
Cuts at: 3416 8394 3416
Size: 4978 5221
SacI G_AGCT'C
Cuts at: 7213 7213
Size: 10199
SacII CC_GC'GG
Cuts at: 6548 6548
Size: 10199
SalI G'TCGA_C
Cuts at: 7236 7236
Size: 10199
SapI GCTCTTCn'nnn_
Cuts at: 4148 4666 8222 8432 4148
Size: 518 3556 210 5915
Fragments arranged by size:
5915 3556 518 210
SbfI CC_TGCA'GG
Cuts at: 7246 7246
Size: 10199
ScaI AGT'ACT
Cuts at: 4080 9716 4080
Size: 5636 4563
SfcI C'TryA_G
Cuts at: 33 674 874 3781 5599 5831 6752 7242 7927
Size: 641 200 2907 1818 232 921 490 685
Cuts at: 7927 8895 9086 33
Size: 968 191 1146
Fragments arranged by size:
2907 1818 1146 968 921 685 641 490
232 200 191
SfoI GGC'GCC
Cuts at: 1447 3776 4112 5435 7631 7879 1447
Size: 2329 336 1323 2196 248 3767
Fragments arranged by size:
3767 2329 2196 1323 336 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10199
SmaI CCC'GGG
Cuts at: 3996 5924 7221 3996
Size: 1928 1297 6974
Fragments arranged by size:
6974 1928 1297
SmlI C'TyrA_G
Cuts at: 299 791 3646 4408 4597 5214 5844 6484 7354
Size: 492 2855 762 189 617 630 640 870
Cuts at: 7354 8269 8983 9245 299
Size: 915 714 262 1253
Fragments arranged by size:
2855 1253 915 870 762 714 640 630
617 492 262 189
SpeI A'CTAG_T
Cuts at: 67 927 67
Size: 860 9339
SphI G_CATG'C
Cuts at: 2876 6798 7252 7752 2876
Size: 3922 454 500 5323
Fragments arranged by size:
5323 3922 500 454
SspI AAT'ATT
Cuts at: 1573 2374 1573
Size: 801 9398
StuI AGG'CCT
Cuts at: 1369 4603 4768 5231 6748 7189 1369
Size: 3234 165 463 1517 441 4379
Fragments arranged by size:
4379 3234 1517 463 441 165
StyI C'CwwG_G
Cuts at: 107 1109 1565 1634 1829 3926 3987 4334 4945
Size: 1002 456 69 195 2097 61 347 611
Cuts at: 4945 5315 5417 5672 6238 6779 6944 7190 7333
Size: 370 102 255 566 541 165 246 143
Cuts at: 7333 7687 107
Size: 354 2619
Fragments arranged by size:
2619 2097 1002 611 566 541 456 370
354 347 255 246 195 165 143 102
69 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 435 2135 3405 4573 5405 6266 7782 7915 8374
Size: 1700 1270 1168 832 861 1516 133 459
Cuts at: 8374 8583 435
Size: 209 2051
Fragments arranged by size:
2051 1700 1516 1270 1168 861 832 459
209 133
TatI w'GTAC_w
Cuts at: 1541 1593 4078 9714 1541
Size: 52 2485 5636 2026
Fragments arranged by size:
5636 2485 2026 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 824 856 1799 1831 2912 2944 3694 3726 3967
Size: 32 943 32 1081 32 750 32 241
Cuts at: 3967 3999 4835 4867 9716 9748 824
Size: 32 836 32 4849 32 1275
Fragments arranged by size:
4849 1275 1081 943 836 750 241 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7996 7996
Size: 10199
XbaI T'CTAG_A
Cuts at: 6168 7230 6168
Size: 1062 9137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3985 6055 6127 6912 7694 3985
Size: 2070 72 785 782 6490
Fragments arranged by size:
6490 2070 785 782 72
XhoI C'TCGA_G
Cuts at: 791 5844 6484 791
Size: 5053 640 4506
Fragments arranged by size:
5053 4506 640
XmaI C'CCGG_G
Cuts at: 3994 5922 7219 3994
Size: 1928 1297 6974
Fragments arranged by size:
6974 1928 1297
XmnI GAAnn'nnTTC
Cuts at: 1787 2099 5579 6562 1787
Size: 312 3480 983 5424
Fragments arranged by size:
5424 3480 983 312
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BfrBI BsiWI BstZ17I CspCI FspAI HpaI MfeI NotI
NruI NsiI PacI PmlI PshAI PsiI SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI ZraI