Restriction map for pF1KA0303
 (Linear) (Six-base) MAPSORT of: KIAA0303.seq  Check: 9854  from: 1  to: 7086



KIAA0303.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AccI GT'mk_AC

Cuts at:      0     542    1169    7086 
   Size:        542     627    5917

  Fragments arranged by size: 

                5917     627     542
 
Acc65I G'GTAC_C

Cuts at:      0    3302    7086 
   Size:       3302    3784
 
AclI AA'CG_TT

Cuts at:      0     623    7086 
   Size:        623    6463
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2285    2462    2800    2881    3832    4045    4107    4354 
   Size:       2285     177     338      81     951     213      62     247

Cuts at:   4354    4630    5114    6362    7086 
   Size:        276     484    1248     724

  Fragments arranged by size: 

                2285    1248     951     724     484     338     276     247
                 213     177      81      62
 
AfeI AGC'GCT

Cuts at:      0    5170    5471    7086 
   Size:       5170     301    1615

  Fragments arranged by size: 

                5170    1615     301
 
AflIII A'CryG_T

Cuts at:      0    3692    7086 
   Size:       3692    3394
 
AgeI A'CCGG_T

Cuts at:      0    3305    5843    6410    7086 
   Size:       3305    2538     567     676

  Fragments arranged by size: 

                3305    2538     676     567
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1220    2245    7086 
   Size:       1220    1025    4841

  Fragments arranged by size: 

                4841    1220    1025
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4825    4857    7086 
   Size:       4825      32    2229

  Fragments arranged by size: 

                4825    2229      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1863    2308    2620    3681    4690    5230    6174    6547 
   Size:       1863     445     312    1061    1009     540     944     373

Cuts at:   6547    7086 
   Size:        539

  Fragments arranged by size: 

                1863    1061    1009     944     540     539     445     373
                 312
 
ApaI G_GGCC'C

Cuts at:      0    4134    5821    6029    6482    7086 
   Size:       4134    1687     208     453     604

  Fragments arranged by size: 

                4134    1687     604     453     208
 
ApaLI G'TGCA_C

Cuts at:      0    2725    7086 
   Size:       2725    4361
 
ApoI r'AATT_y

Cuts at:      0     207     877     892    1741    1987    2178    4869    5411 
   Size:        207     670      15     849     246     191    2691     542

Cuts at:   5411    7086 
   Size:       1675

  Fragments arranged by size: 

                2691    1675     849     670     542     246     207     191
                  15
 
AseI AT'TA_AT

Cuts at:      0    1220    7086 
   Size:       1220    5866
 
AvaI C'yCGr_G

Cuts at:      0     683    1112    3886    5736    5814    6288    6376    7086 
   Size:        683     429    2774    1850      78     474      88     710

  Fragments arranged by size: 

                2774    1850     710     683     474     429      88      78
            
 
BamHI G'GATC_C

Cuts at:      0     996    7086 
   Size:        996    6090
 
BanI G'GyrC_C

Cuts at:      0    1337    1839    3302    3666    4002    4089    4144    5325 
   Size:       1337     502    1463     364     336      87      55    1181

Cuts at:   5325    5560    7086 
   Size:        235    1526

  Fragments arranged by size: 

                1526    1463    1337    1181     502     364     336     235
                  87      55
 
BanII G_rGCy'C

Cuts at:      0     490    3521    4134    4412    4570    4702    4945    5204 
   Size:        490    3031     613     278     158     132     243     259

Cuts at:   5204    5732    5743    5821    6029    6057    6482    6867    7086 
   Size:        528      11      78     208      28     425     385     219

  Fragments arranged by size: 

                3031     613     528     490     425     385     278     259
                 243     219     208     158     132      78      28      11
            
 
BbeI G_GCGC'C

Cuts at:      0    1341    3670    4006    5329    7086 
   Size:       1341    2329     336    1323    1757

  Fragments arranged by size: 

                2329    1757    1341    1323     336
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2099    2204    7086 
   Size:       2099     105    4882

  Fragments arranged by size: 

                4882    2099     105
 
BbvCI CC'TCA_GC

Cuts at:      0     225    2610    6540    7086 
   Size:        225    2385    3930     546

  Fragments arranged by size: 

                3930    2385     546     225
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    5155    5189    7086 
   Size:       5155      34    1897

  Fragments arranged by size: 

                5155    1897      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1767    7086 
   Size:       1767    5319
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1162    4112    7086 
   Size:       1162    2950    2974

  Fragments arranged by size: 

                2974    2950    1162
 
BglII A'GATC_T

Cuts at:      0    2790    3007    7086 
   Size:       2790     217    4079

  Fragments arranged by size: 

                4079    2790     217
 
BlpI GC'TnA_GC

Cuts at:      0    3712    6731    7086 
   Size:       3712    3019     355

  Fragments arranged by size: 

                3712    3019     355
 
Bme1580I G_kGCm'C

Cuts at:      0    1535    2454    2729    3225    3251    4134    4627    5821 
   Size:       1535     919     275     496      26     883     493    1194

Cuts at:   5821    6029    6482    7086 
   Size:        208     453     604

  Fragments arranged by size: 

                1535    1194     919     883     604     496     493     453
                 275     208      26
 
BmgBI CAC'GTC

Cuts at:      0    3702    4719    6960    7086 
   Size:       3702    1017    2241     126

  Fragments arranged by size: 

                3702    2241    1017     126
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     212     991    3436    3748    4375    6557    7086 
   Size:        212     779    2445     312     627    2182     529

  Fragments arranged by size: 

                2445    2182     779     627     529     312     212
 
BmtI G_CTAG'C

Cuts at:      0    3028    7086 
   Size:       3028    4058
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3026    4233    5004    5751    7086 
   Size:       3026    1207     771     747    1335

  Fragments arranged by size: 

                3026    1335    1207     771     747
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1763    1915    2274    2420    2807    3640    4135    4849 
   Size:       1763     152     359     146     387     833     495     714

Cuts at:   4849    4852    5517    6121    6130    6341    6588    6712    6829 
   Size:          3     665     604       9     211     247     124     117

Cuts at:   6829    7086 
   Size:        257

  Fragments arranged by size: 

                1763     833     714     665     604     495     387     359
                 257     247     211     152     146     124     117       9
                   3
 
Bpu10I CC'TnA_GC

Cuts at:      0     225     633    2610    6540    7086 
   Size:        225     408    1977    3930     546

  Fragments arranged by size: 

                3930    1977     546     408     225
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176    3523    4321    4474    5091    7086 
   Size:        176    3347     798     153     617    1995

  Fragments arranged by size: 

                3347    1995     798     617     176     153
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     330    2396    3146    4165    4867    5492    6798    7086 
   Size:        330    2066     750    1019     702     625    1306     288

  Fragments arranged by size: 

                2066    1306    1019     750     702     625     330     288
            
 
BsaBI GATnn'nnATC

Cuts at:      0    2644    7086 
   Size:       2644    4442
 
BsaHI Gr'CG_yC

Cuts at:      0     261    1338    3361    3667    4003    5326    6065    7086 
   Size:        261    1077    2023     306     336    1323     739    1021

  Fragments arranged by size: 

                2023    1323    1077    1021     739     336     306     261
            
 
BsaWI w'CCGG_w

Cuts at:      0     107     885    3305    3310    4648    5843    6410    7086 
   Size:        107     778    2420       5    1338    1195     567     676

  Fragments arranged by size: 

                2420    1338    1195     778     676     567     107       5
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2804    2834    3351    3381    3550    3580    3586    3616 
   Size:       2804      30     517      30     169      30       6      30

Cuts at:   3616    4727    4757    4825    4855    6189    6219    7086 
   Size:       1111      30      68      30    1334      30     867

  Fragments arranged by size: 

                2804    1334    1111     867     517     169      68      30
                  30      30      30      30      30      30       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75    1798    1867    2428    2473    3018    3571    3649 
   Size:         75    1723      69     561      45     545     553      78

Cuts at:   3649    5251    5382    5844    6047    6591    7086 
   Size:       1602     131     462     203     544     495

  Fragments arranged by size: 

                1723    1602     561     553     545     544     495     462
                 203     131      78      75      69      45
 
BseYI C'CCAG_C

Cuts at:      0     313    1902    2478    2596    3234    3259    3457    3656 
   Size:        313    1589     576     118     638      25     198     199

Cuts at:   3656    4011    4702    4747    4852    5430    5605    5716    5731 
   Size:        355     691      45     105     578     175     111      15

Cuts at:   5731    6706    6915    6920    6930    6975    7086 
   Size:        975     209       5      10      45     111

  Fragments arranged by size: 

                1589     975     691     638     578     576     355     313
                 209     199     198     175     118     111     111     105
                  45      45      25      15      10       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3568    3661    4676    5383    6025    7086 
   Size:       3568      93    1015     707     642    1061

  Fragments arranged by size: 

                3568    1061    1015     707     642      93
 
BsiEI CG_ry'CG

Cuts at:      0    4619    5844    6302    7086 
   Size:       4619    1225     458     784

  Fragments arranged by size: 

                4619    1225     784     458
 
BsiHKAI G_wGCw'C

Cuts at:      0      35    2729    3604    4103    7086 
   Size:         35    2694     875     499    2983

  Fragments arranged by size: 

                2983    2694     875     499      35
 
BsmI GAATG_Cn'

Cuts at:      0    4369    7086 
   Size:       4369    2717
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1438    4479    6950    7086 
   Size:       1438    3041    2471     136

  Fragments arranged by size: 

                3041    2471    1438     136
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     490    1535    2454    2729    3225    3251    3521 
   Size:         35     455    1045     919     275     496      26     270

Cuts at:   3521    3604    4103    4134    4412    4570    4627    4702    4945 
   Size:         83     499      31     278     158      57      75     243

Cuts at:   4945    5204    5732    5743    5821    6029    6057    6482    6867 
   Size:        259     528      11      78     208      28     425     385

Cuts at:   6867    7086 
   Size:        219

  Fragments arranged by size: 

                1045     919     528     499     496     455     425     385
                 278     275     270     259     243     219     208     158
                  83      78      75      57      35      31      28      26
                  11
 
BspEI T'CCGG_A

Cuts at:      0     885    3310    4648    7086 
   Size:        885    2425    1338    2438

  Fragments arranged by size: 

                2438    2425    1338     885
 
BspHI T'CATG_A

Cuts at:      0     777    7086 
   Size:        777    6309
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    4835    5041    7086 
   Size:       4835     206    2045

  Fragments arranged by size: 

                4835    2045     206
 
BsrBI CCG'CTC

Cuts at:      0    1272    3161    3711    4138    5085    6429    7086 
   Size:       1272    1889     550     427     947    1344     657

  Fragments arranged by size: 

                1889    1344    1272     947     657     550     427
 
BsrDI GCAATG_nn'

Cuts at:      0     132     142    1155    1664    4634    5667    7086 
   Size:        132      10    1013     509    2970    1033    1419

  Fragments arranged by size: 

                2970    1419    1033    1013     509     132      10
 
BsrFI r'CCGG_y

Cuts at:      0    1704    3305    3328    4416    5843    6297    6382    6410 
   Size:       1704    1601      23    1088    1427     454      85      28

Cuts at:   6410    7086 
   Size:        676

  Fragments arranged by size: 

                1704    1601    1427    1088     676     454      85      28
                  23
 
BsrGI T'GTAC_A

Cuts at:      0    1485    7086 
   Size:       1485    5601
 
BssHII G'CGCG_C

Cuts at:      0    4056    7086 
   Size:       4056    3030
 
BssSI C'ACGA_G

Cuts at:      0    2582    7086 
   Size:       2582    4504
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1319    2620    6352    7086 
   Size:       1319    1301    3732     734

  Fragments arranged by size: 

                3732    1319    1301     734
 
BstBI TT'CG_AA

Cuts at:      0     825    1101    1242    7086 
   Size:        825     276     141    5844

  Fragments arranged by size: 

                5844     825     276     141
 
BstEII G'GTnAC_C

Cuts at:      0    1518    2469    3398    3624    7086 
   Size:       1518     951     929     226    3462

  Fragments arranged by size: 

                3462    1518     951     929     226
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3825    5353    5692    7086 
   Size:       3825    1528     339    1394

  Fragments arranged by size: 

                3825    1528    1394     339
 
BstYI r'GATC_y

Cuts at:      0     830     996    1250    1860    2790    3007    3082    7086 
   Size:        830     166     254     610     930     217      75    4004

  Fragments arranged by size: 

                4004     930     830     610     254     217     166      75
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1879    2465    4655    5125    6461    7086 
   Size:       1879     586    2190     470    1336     625

  Fragments arranged by size: 

                2190    1879    1336     625     586     470
 
BtgI C'CryG_G

Cuts at:      0    1526    1721    3270    3879    4391    5207    5309    6437 
   Size:       1526     195    1549     609     512     816     102    1128

Cuts at:   6437    6569    6836    7086 
   Size:        132     267     250

  Fragments arranged by size: 

                1549    1526    1128     816     609     512     267     250
                 195     132     102
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     414    4087    6014    7086 
   Size:        414    3673    1927    1072

  Fragments arranged by size: 

                3673    1927    1072     414
 
BtsI GCAGTG_nn'

Cuts at:      0    3588    4761    5720    6037    7086 
   Size:       3588    1173     959     317    1049

  Fragments arranged by size: 

                3588    1173    1049     959     317
 
ClaI AT'CG_AT

Cuts at:      0    1938    4274    7086 
   Size:       1938    2336    2812

  Fragments arranged by size: 

                2812    2336    1938
 
DraIII CAC_nnn'GTG

Cuts at:      0    3498    6251    7086 
   Size:       3498    2753     835

  Fragments arranged by size: 

                3498    2753     835
 
EaeI y'GGCC_r

Cuts at:      0    1424    3724    4265    5066    5633    6009    6299    6430 
   Size:       1424    2300     541     801     567     376     290     131

Cuts at:   6430    7086 
   Size:        656

  Fragments arranged by size: 

                2300    1424     801     656     567     541     376     290
                 131
 
EagI C'GGCC_G

Cuts at:      0    6299    7086 
   Size:       6299     787
 
EarI CTCTTCn'nnn_

Cuts at:      0     333    2183    2304    2438    2857    3416    3920    4040 
   Size:        333    1850     121     134     419     559     504     120

Cuts at:   4040    4558    5512    6669    7086 
   Size:        518     954    1157     417

  Fragments arranged by size: 

                1850    1157     954     559     518     504     419     417
                 333     134     121     120
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2979    3315    5609    6337    7086 
   Size:       2979     336    2294     728     749

  Fragments arranged by size: 

                2979    2294     749     728     336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1763    1915    2274    2285    2420    2462    2800    2807 
   Size:       1763     152     359      11     135      42     338       7

Cuts at:   2807    2881    3640    3832    4045    4107    4135    4354    4630 
   Size:         74     759     192     213      62      28     219     276

Cuts at:   4630    4849    4852    5114    5517    6121    6130    6341    6362 
   Size:        219       3     262     403     604       9     211      21

Cuts at:   6362    6588    6712    6829    7086 
   Size:        226     124     117     257

  Fragments arranged by size: 

                1763     759     604     403     359     338     276     262
                 257     226     219     219     213     211     192     152
                 135     124     117      74      62      42      28      21
                  11       9       7       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1597    4024    7086 
   Size:       1597    2427    3062

  Fragments arranged by size: 

                3062    2427    1597
 
EcoO109I rG'GnC_Cy

Cuts at:      0     711    1405    3527    4131    4724    4921    5146    5276 
   Size:        711     694    2122     604     593     197     225     130

Cuts at:   5276    5372    5748    5818    6026    6148    6478    6906    7086 
   Size:         96     376      70     208     122     330     428     180

  Fragments arranged by size: 

                2122     711     694     604     593     428     376     330
                 225     208     197     180     130     122      96      70
            
 
EcoRI G'AATT_C

Cuts at:      0    2178    4869    5411    7086 
   Size:       2178    2691     542    1675

  Fragments arranged by size: 

                2691    2178    1675     542
 
EcoRV GAT'ATC

Cuts at:      0    1278    7086 
   Size:       1278    5808
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1017    4551    5491    5705    7086 
   Size:       1017    3534     940     214    1381

  Fragments arranged by size: 

                3534    1381    1017     940     214
 
FseI GG_CCGG'CC

Cuts at:      0    6386    7086 
   Size:       6386     700
 
FspI TGC'GCA

Cuts at:      0    5771    7086 
   Size:       5771    1315
 
HaeII r_GCGC'y

Cuts at:      0    1341    3670    4006    5172    5329    5473    7086 
   Size:       1341    2329     336    1166     157     144    1613

  Fragments arranged by size: 

                2329    1613    1341    1166     336     157     144
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1787    1819    2023    2055    2312    2344    2780    2812 
   Size:       1787      32     204      32     257      32     436      32

Cuts at:   2812    3163    3195    3351    3383    3550    3582    4424    4456 
   Size:        351      32     156      32     167      32     842      32

Cuts at:   4456    5377    5409    7086 
   Size:        921      32    1677

  Fragments arranged by size: 

                1787    1677     921     842     436     351     257     204
                 167     156      32      32      32      32      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     114    5023    5096    7086 
   Size:        114    4909      73    1990

  Fragments arranged by size: 

                4909    1990     114      73
 
KasI G'GCGC_C

Cuts at:      0    1337    3666    4002    5325    7086 
   Size:       1337    2329     336    1323    1761

  Fragments arranged by size: 

                2329    1761    1337    1323     336
 
KpnI G_GTAC'C

Cuts at:      0    3306    7086 
   Size:       3306    3780
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1731    2318    3169    3394    4058    4410    7086 
   Size:       1731     587     851     225     664     352    2676

  Fragments arranged by size: 

                2676    1731     851     664     587     352     225
 
MscI TGG'CCA

Cuts at:      0    1426    4267    5068    6011    7086 
   Size:       1426    2841     801     943    1075

  Fragments arranged by size: 

                2841    1426    1075     943     801
 
MslI CAynn'nnrTG

Cuts at:      0     460    1354    1475    2692    2807    5351    7086 
   Size:        460     894     121    1217     115    2544    1735

  Fragments arranged by size: 

                2544    1735    1217     894     460     121     115
 
MspA1I CmG'CkG

Cuts at:      0     266    3300    3351    3396    3534    3645    3660    3672 
   Size:        266    3034      51      45     138     111      15      12

Cuts at:   3672    4011    4351    5407    6163    6181    6439    7086 
   Size:        339     340    1056     756      18     258     647

  Fragments arranged by size: 

                3034    1056     756     647     340     339     266     258
                 138     111      51      45      18      15      12
 
NaeI GCC'GGC

Cuts at:      0    3330    4418    6299    6384    7086 
   Size:       3330    1088    1881      85     702

  Fragments arranged by size: 

                3330    1881    1088     702      85
 
NarI GG'CG_CC

Cuts at:      0    1338    3667    4003    5326    7086 
   Size:       1338    2329     336    1323    1760

  Fragments arranged by size: 

                2329    1760    1338    1323     336
 
NcoI C'CATG_G

Cuts at:      0    1526    1721    3879    5207    5309    6836    7086 
   Size:       1526     195    2158    1328     102    1527     250

  Fragments arranged by size: 

                2158    1527    1526    1328     250     195     102
 
NdeI CA'TA_TG

Cuts at:      0    1920    2057    7086 
   Size:       1920     137    5029

  Fragments arranged by size: 

                5029    1920     137
 
NgoMIV G'CCGG_C

Cuts at:      0    3328    4416    6297    6382    7086 
   Size:       3328    1088    1881      85     704

  Fragments arranged by size: 

                3328    1881    1088     704      85
 
NheI G'CTAG_C

Cuts at:      0    3024    7086 
   Size:       3024    4062
 
NspI r_CATG'y

Cuts at:      0    1317    2768    5195    6690    7086 
   Size:       1317    1451    2427    1495     396

  Fragments arranged by size: 

                2427    1495    1451    1317     396
 
PasI CC'CwG_GG

Cuts at:      0    6145    7086 
   Size:       6145     941
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      23    1768    2824    5310    7086 
   Size:         23    1745    1056    2486    1776

  Fragments arranged by size: 

                2486    1776    1745    1056      23
 
PfoI T'CCnGG_A

Cuts at:      0    1631    3760    4151    6578    7086 
   Size:       1631    2129     391    2427     508

  Fragments arranged by size: 

                2427    2129    1631     508     391
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2241    2273    4825    4857    7086 
   Size:       2241      32    2552      32    2229

  Fragments arranged by size: 

                2552    2241    2229      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1405    3527    4921    5276    5748    6906    7086 
   Size:       1405    2122    1394     355     472    1158     180

  Fragments arranged by size: 

                2122    1405    1394    1158     472     355     180
 
PspOMI G'GGCC_C

Cuts at:      0    4130    5817    6025    6478    7086 
   Size:       4130    1687     208     453     608

  Fragments arranged by size: 

                4130    1687     608     453     208
 
PspXI vC'TCGA_Gb

Cuts at:      0    5736    6376    7086 
   Size:       5736     640     710

  Fragments arranged by size: 

                5736     710     640
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     526     558    7086 
   Size:        526      32    6528

  Fragments arranged by size: 

                6528     526      32
 
PstI C_TGCA'G

Cuts at:      0    3677    5495    5727    6648    7086 
   Size:       3677    1818     232     921     438

  Fragments arranged by size: 

                3677    1818     921     438     232
 
PvuI CG_AT'CG

Cuts at:      0    4619    7086 
   Size:       4619    2467
 
PvuII CAG'CTG

Cuts at:      0    4351    5407    7086 
   Size:       4351    1056    1679

  Fragments arranged by size: 

                4351    1679    1056
 
RsrII CG'GwC_CG

Cuts at:      0    3308    7086 
   Size:       3308    3778
 
SacII CC_GC'GG

Cuts at:      0    6440    7086 
   Size:       6440     646
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4040    4558    7086 
   Size:       4040     518    2528

  Fragments arranged by size: 

                4040    2528     518
 
ScaI AGT'ACT

Cuts at:      0    3972    7086 
   Size:       3972    3114
 
SfcI C'TryA_G

Cuts at:      0     566     766    3673    5491    5723    6644    7086 
   Size:        566     200    2907    1818     232     921     442

  Fragments arranged by size: 

                2907    1818     921     566     442     232     200
 
SfoI GGC'GCC

Cuts at:      0    1339    3668    4004    5327    7086 
   Size:       1339    2329     336    1323    1759

  Fragments arranged by size: 

                2329    1759    1339    1323     336
 
SmaI CCC'GGG

Cuts at:      0    3888    5816    7086 
   Size:       3888    1928    1270

  Fragments arranged by size: 

                3888    1928    1270
 
SmlI C'TyrA_G

Cuts at:      0     191     683    3538    4300    4489    5106    5736    6376 
   Size:        191     492    2855     762     189     617     630     640

Cuts at:   6376    7086 
   Size:        710

  Fragments arranged by size: 

                2855     762     710     640     630     617     492     191
                 189
 
SpeI A'CTAG_T

Cuts at:      0     819    7086 
   Size:        819    6267
 
SphI G_CATG'C

Cuts at:      0    2768    6690    7086 
   Size:       2768    3922     396

  Fragments arranged by size: 

                3922    2768     396
 
SspI AAT'ATT

Cuts at:      0    1465    2266    7086 
   Size:       1465     801    4820

  Fragments arranged by size: 

                4820    1465     801
 
StuI AGG'CCT

Cuts at:      0    1261    4495    4660    5123    6640    7081    7086 
   Size:       1261    3234     165     463    1517     441       5

  Fragments arranged by size: 

                3234    1517    1261     463     441     165       5
 
StyI C'CwwG_G

Cuts at:      0    1001    1457    1526    1721    3818    3879    4226    4837 
   Size:       1001     456      69     195    2097      61     347     611

Cuts at:   4837    5207    5309    5564    6130    6671    6836    7086 
   Size:        370     102     255     566     541     165     250

  Fragments arranged by size: 

                2097    1001     611     566     541     456     370     347
                 255     250     195     165     102      69      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     327    2027    3297    4465    5297    6158    7086 
   Size:        327    1700    1270    1168     832     861     928

  Fragments arranged by size: 

                1700    1270    1168     928     861     832     327
 
TatI w'GTAC_w

Cuts at:      0    1433    1485    3970    7086 
   Size:       1433      52    2485    3116

  Fragments arranged by size: 

                3116    2485    1433      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     716     748    1691    1723    2804    2836    3586    3618 
   Size:        716      32     943      32    1081      32     750      32

Cuts at:   3618    3859    3891    4727    4759    7086 
   Size:        241      32     836      32    2327

  Fragments arranged by size: 

                2327    1081     943     836     750     716     241      32
                  32      32      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    6060    7086 
   Size:       6060    1026
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3877    5947    6019    6804    7086 
   Size:       3877    2070      72     785     282

  Fragments arranged by size: 

                3877    2070     785     282      72
 
XhoI C'TCGA_G

Cuts at:      0     683    5736    6376    7086 
   Size:        683    5053     640     710

  Fragments arranged by size: 

                5053     710     683     640
 
XmaI C'CCGG_G

Cuts at:      0    3886    5814    7086 
   Size:       3886    1928    1272

  Fragments arranged by size: 

                3886    1928    1272
 
XmnI GAAnn'nnTTC

Cuts at:      0    1679    1991    5471    6454    7086 
   Size:       1679     312    3480     983     632

  Fragments arranged by size: 

                3480    1679     983     632     312
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    HaeII
    Hin4I  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NspI     PasI
    PflMI     PfoI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SapI     ScaI     SfcI     SfoI
     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AscI    AsiSI    AvrII
     BaeI     BclI    BfrBI    BsaAI    BsiWI  BstZ17I    CspCI     DraI
     DrdI  EcoICRI    FspAI   HincII     HpaI     MfeI     MluI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI    PshAI     PsiI
     SacI     SalI    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SrfI     SwaI  Tth111I     ZraI
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