Restriction map for pF1KA0284
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# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        Alw44I      ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     BciVI       
BclI        BfrI        BglII       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfuI        SgrAI       
SnaBI       Sse8387I    SspBI       SspI        SwaI        
Tru9I       XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   26 enzymes) #
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AcsI         4476  GAATTT               [GA]^AATT[CT]
AhdI         2632  GACGCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1040  AGCGCT               AGC^GCT
ApoI         4476  GAATTT               [GA]^AATT[CT]
AspEI        2632  GACGCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1555  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2253  CCTAGG               C^CTAGG
BamHI        2016  GGATCC               G^GATCC
BbrPI        2121  CACGTG               CAC^GTG
BlnI         2253  CCTAGG               C^CTAGG
BsaAI        2121  CACGTG               [CT]AC^GT[GA]
BspLU11I       71  ACATGT               A^CATGT
DrdI          811  GACCTGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2632  GACGCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1996  CTCTTC               CTCTTC(1/4)
Eco47III     1040  AGCGCT               AGC^GCT
FseI         3250  GGCCGGCC             GGCCGG^CC
Ksp632I      1996  CTCTTC               CTCTTC(1/4)
PflFI        1555  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2121  CACGTG               CAC^GTG
PmlI         2121  CACGTG               CAC^GTG
SexAI        1556  ACCAGGT              A^CC[AT]GGT
SpeI         3337  ACTAGT               A^CTAGT
SphI         1877  GCATGC               GCATG^C
TfiI           64  GATTC                G^A[AT]TC
Tth111I      1555  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


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# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflIII         71  ACATGT               A^C[GA][CT]GT
             3560  ACACGT              
AviII           2  TGCGCA               TGC^GCA
             1874  TGCGCA              
BbsI         1200  GAAGAC               GAAGAC(2/6)
             4245  GAAGAC              
BcgI          758  CGAGCCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2358  CGACGGCCCTGC        
BpuAI        1200  GAAGAC               GAAGAC(2/6)
             4245  GAAGAC              
BsaBI         630  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4161  GATGAAGATC          
BsmBI        2536  GAGACG               CGTCTC(1/5)
             2542  GAGACG              
BstAPI       3352  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3751  GCAGAGATTGC         
BstXI        1394  CCAGCCCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1557  CCAGGTCTTTGG        
DraIII       1703  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3823  CACGATGTG           
FspI            2  TGCGCA               TGC^GCA
             1874  TGCGCA              
MamI          630  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4161  GATGAAGATC          
MslI           72  CATGTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1524  CACGGAGGTG          
NcoI         1386  CCATGG               C^CATGG
             3419  CCATGG              
SacI          400  GAGCTC               GAGCT^C
             1320  GAGCTC              
SmlI          118  CTCAAG               C^T[CT][GA]AG
             1189  CTCAAG              
Tsp509I      2581  AATT                 ^AATT
             4477  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        SfoI        SmaI        StuI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        
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