Restriction map for pF1KA0284
 (Linear) (Six-base) MAPSORT of: KIAA0284.seq  Check: 9035  from: 1  to: 4557



KIAA0284.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 16:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     138     541     927    4557 
   Size:        138     403     386    3630

  Fragments arranged by size: 

                3630     403     386     138
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     856    3176    3474    4264    4297    4557 
   Size:         52     804    2320     298     790      33     260

  Fragments arranged by size: 

                2320     804     790     298     260      52      33
 
AfeI AGC'GCT

Cuts at:      0    1042    4557 
   Size:       1042    3515
 
AflIII A'CryG_T

Cuts at:      0      71    3560    4557 
   Size:         71    3489     997

  Fragments arranged by size: 

                3489     997      71
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2637    4557 
   Size:       2637    1920
 
AleI CACnn'nnGTG

Cuts at:      0    1528    4557 
   Size:       1528    3029
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1706    4557 
   Size:       1706    2851
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4093    4125    4557 
   Size:       4093      32     432

  Fragments arranged by size: 

                4093     432      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1311    2472    2724    3199    3262    3358    3786    4557 
   Size:       1311    1161     252     475      63      96     428     771

  Fragments arranged by size: 

                1311    1161     771     475     428     252      96      63
            
 
ApaI G_GGCC'C

Cuts at:      0    1408    1933    2120    2154    2702    3177    3501    3684 
   Size:       1408     525     187      34     548     475     324     183

Cuts at:   3684    4557 
   Size:        873

  Fragments arranged by size: 

                1408     873     548     525     475     324     187     183
                  34
 
ApoI r'AATT_y

Cuts at:      0    4476    4557 
   Size:       4476      81
 
AvaI C'yCGr_G

Cuts at:      0     508     681     756    1164    1345    1812    2065    2155 
   Size:        508     173      75     408     181     467     253      90

Cuts at:   2155    2483    2616    2772    2900    3062    3346    3504    3645 
   Size:        328     133     156     128     162     284     158     141

Cuts at:   3645    3677    3692    3923    3949    3998    4557 
   Size:         32      15     231      26      49     559

  Fragments arranged by size: 

                 559     508     467     408     328     284     253     231
                 181     173     162     158     156     141     133     128
                  90      75      49      32      26      15
 
AvrII C'CTAG_G

Cuts at:      0    2253    4557 
   Size:       2253    2304
 
BamHI G'GATC_C

Cuts at:      0    2016    4557 
   Size:       2016    2541
 
BanI G'GyrC_C

Cuts at:      0     168     513     912    1271    1743    2779    3536    3975 
   Size:        168     345     399     359     472    1036     757     439

Cuts at:   3975    4233    4557 
   Size:        258     324

  Fragments arranged by size: 

                1036     757     472     439     399     359     345     324
                 258     168
 
BanII G_rGCy'C

Cuts at:      0     359     404     458    1324    1408    1753    1933    2107 
   Size:        359      45      54     866      84     345     180     174

Cuts at:   2107    2120    2154    2702    3177    3245    3501    3553    3672 
   Size:         13      34     548     475      68     256      52     119

Cuts at:   3672    3684    3856    4426    4557 
   Size:         12     172     570     131

  Fragments arranged by size: 

                 866     570     548     475     359     345     256     180
                 174     172     131     119      84      68      54      52
                  45      34      13      12
 
BbeI G_GCGC'C

Cuts at:      0     172    1275    3540    4557 
   Size:        172    1103    2265    1017

  Fragments arranged by size: 

                2265    1103    1017     172
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1207    4252    4557 
   Size:       1207    3045     305

  Fragments arranged by size: 

                3045    1207     305
 
BbvCI CC'TCA_GC

Cuts at:      0    1895    2029    3166    3883    4440    4557 
   Size:       1895     134    1137     717     557     117

  Fragments arranged by size: 

                1895    1137     717     557     134     117
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     747     781    2347    2381    4557 
   Size:        747      34    1566      34    2176

  Fragments arranged by size: 

                2176    1566     747      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     895    1403    1720    1792    1928    2000    2554    2563 
   Size:        895     508     317      72     136      72     554       9

Cuts at:   2563    2868    3004    4557 
   Size:        305     136    1553

  Fragments arranged by size: 

                1553     895     554     508     317     305     136     136
                  72      72       9
 
BlpI GC'TnA_GC

Cuts at:      0     425    2874    3084    3263    3775    4557 
   Size:        425    2449     210     179     512     782

  Fragments arranged by size: 

                2449     782     512     425     210     179
 
Bme1580I G_kGCm'C

Cuts at:      0     917    1408    1933    2120    2154    2702    2733    2799 
   Size:        917     491     525     187      34     548      31      66

Cuts at:   2799    3177    3501    3516    3684    4238    4557 
   Size:        378     324      15     168     554     319

  Fragments arranged by size: 

                 917     554     548     525     491     378     324     319
                 187     168      66      34      31      15
 
BmgBI CAC'GTC

Cuts at:      0    2655    3563    3798    4557 
   Size:       2655     908     235     759

  Fragments arranged by size: 

                2655     908     759     235
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1041    1121    1135    1299    1612    1714    1898    2915 
   Size:       1041      80      14     164     313     102     184    1017

Cuts at:   2915    3859    4328    4557 
   Size:        944     469     229

  Fragments arranged by size: 

                1041    1017     944     469     313     229     184     164
                 102      80      14
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     285    1710    2826    3603    4557 
   Size:        285    1425    1116     777     954

  Fragments arranged by size: 

                1425    1116     954     777     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103    1575    2219    2433    2581    3426    3645    4557 
   Size:        103    1472     644     214     148     845     219     912

  Fragments arranged by size: 

                1472     912     845     644     219     214     148     103
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1895    2029    3166    3239    3883    4440    4557 
   Size:       1895     134    1137      73     644     557     117

  Fragments arranged by size: 

                1895    1137     644     557     134     117      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103    1174    4557 
   Size:        103    1071    3383

  Fragments arranged by size: 

                3383    1071     103
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     243     478     715    1501    4557 
   Size:        243     235     237     786    3056

  Fragments arranged by size: 

                3056     786     243     237     235
 
BsaAI yAC'GTr

Cuts at:      0    2123    4557 
   Size:       2123    2434
 
BsaBI GATnn'nnATC

Cuts at:      0     634    4165    4557 
   Size:        634    3531     392

  Fragments arranged by size: 

                3531     634     392
 
BsaHI Gr'CG_yC

Cuts at:      0     169     338    1272    2294    2633    3537    4037    4557 
   Size:        169     169     934    1022     339     904     500     520

  Fragments arranged by size: 

                1022     934     904     520     500     339     169     169
            
 
BsaWI w'CCGG_w

Cuts at:      0     245    1359    1965    4557 
   Size:        245    1114     606    2592

  Fragments arranged by size: 

                2592    1114     606     245
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2118    2148    2491    2521    2849    2879    3601    3631 
   Size:       2118      30     343      30     328      30     722      30

Cuts at:   3631    3862    3892    4557 
   Size:        231      30     665

  Fragments arranged by size: 

                2118     722     665     343     328     231      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     192    1480    1549    1962    2023    2563    2941 
   Size:        124      68    1288      69     413      61     540     378

Cuts at:   2941    3082    3589    3592    3727    3730    3846    3943    4087 
   Size:        141     507       3     135       3     116      97     144

Cuts at:   4087    4557 
   Size:        470

  Fragments arranged by size: 

                1288     540     507     470     413     378     144     141
                 135     124     116      97      69      68      61       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     341     457     907    1134    1215    1339    1439    1664 
   Size:        341     116     450     227      81     124     100     225

Cuts at:   1664    1781    1987    2267    2272    2280    2370    2421    2701 
   Size:        117     206     280       5       8      90      51     280

Cuts at:   2701    2717    2956    3054    3180    3832    3895    4112    4395 
   Size:         16     239      98     126     652      63     217     283

Cuts at:   4395    4507    4557 
   Size:        112      50

  Fragments arranged by size: 

                 652     450     341     283     280     280     239     227
                 225     217     206     126     124     117     116     112
                 100      98      90      81      63      51      50      16
                   8       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     406     598     751     766    2747    3763    3958 
   Size:        112     294     192     153      15    1981    1016     195

Cuts at:   3958    4315    4367    4557 
   Size:        357      52     190

  Fragments arranged by size: 

                1981    1016     357     294     195     192     190     153
                 112      52      15
 
BsiEI CG_ry'CG

Cuts at:      0     905    1112    1331    4557 
   Size:        905     207     219    3226

  Fragments arranged by size: 

                3226     905     219     207
 
BsiHKAI G_wGCw'C

Cuts at:      0     404    1324    1730    4148    4557 
   Size:        404     920     406    2418     409

  Fragments arranged by size: 

                2418     920     409     406     404
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2530    2536    4557 
   Size:       2530       6    2021

  Fragments arranged by size: 

                2530    2021       6
 
Bsp1286I G_dGCh'C

Cuts at:      0     359     404     458     917    1324    1408    1730    1753 
   Size:        359      45      54     459     407      84     322      23

Cuts at:   1753    1933    2107    2120    2154    2702    2733    2799    3177 
   Size:        180     174      13      34     548      31      66     378

Cuts at:   3177    3245    3501    3516    3553    3672    3684    3856    4148 
   Size:         68     256      15      37     119      12     172     292

Cuts at:   4148    4238    4426    4557 
   Size:         90     188     131

  Fragments arranged by size: 

                 548     459     407     378     359     322     292     256
                 188     180     174     172     131     119      90      84
                  68      66      54      45      37      34      31      23
                  15      13      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     138     541     821     927    4358    4557 
   Size:        138     403     280     106    3431     199

  Fragments arranged by size: 

                3431     403     280     199     138     106
 
BsrBI CCG'CTC

Cuts at:      0     565    1277    1372    2696    3122    3219    3503    4557 
   Size:        565     712      95    1324     426      97     284    1054

  Fragments arranged by size: 

                1324    1054     712     565     426     284      97      95
            
 
BsrFI r'CCGG_y

Cuts at:      0     516    1107    1180    2862    3251    3319    3539    4557 
   Size:        516     591      73    1682     389      68     220    1018

  Fragments arranged by size: 

                1682    1018     591     516     389     220      73      68
            
 
BssSI C'ACGA_G

Cuts at:      0     523     850    3531    4557 
   Size:        523     327    2681    1026

  Fragments arranged by size: 

                2681    1026     523     327
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3358    3757    4557 
   Size:       3358     399     800

  Fragments arranged by size: 

                3358     800     399
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1401    1564    4557 
   Size:       1401     163    2993

  Fragments arranged by size: 

                2993    1401     163
 
BstYI r'GATC_y

Cuts at:      0     470    2016    3818    4166    4557 
   Size:        470    1546    1802     348     391

  Fragments arranged by size: 

                1802    1546     470     391     348
 
Bsu36I CC'TnA_GG

Cuts at:      0     431    2993    4471    4557 
   Size:        431    2562    1478      86

  Fragments arranged by size: 

                2562    1478     431      86
 
BtgI C'CryG_G

Cuts at:      0     405    1095    1386    1400    1430    2853    3245    3419 
   Size:        405     690     291      14      30    1423     392     174

Cuts at:   3419    3470    3594    4557 
   Size:         51     124     963

  Fragments arranged by size: 

                1423     963     690     405     392     291     174     124
                  51      30      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2048    4557 
   Size:       2048    2509
 
BtsI GCAGTG_nn'

Cuts at:      0     187    3390    3516    4557 
   Size:        187    3203     126    1041

  Fragments arranged by size: 

                3203    1041     187     126
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1077    1112    4557 
   Size:       1077      35    3445

  Fragments arranged by size: 

                3445    1077      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1708    3828    4557 
   Size:       1708    2120     729

  Fragments arranged by size: 

                2120    1708     729
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     817    4557 
   Size:        817    3740
 
EaeI y'GGCC_r

Cuts at:      0     731     887     902    1109    1328    1604    1699    2001 
   Size:        731     156      15     207     219     276      95     302

Cuts at:   2001    2474    2889    3253    3299    3378    3422    3800    4043 
   Size:        473     415     364      46      79      44     378     243

Cuts at:   4043    4373    4557 
   Size:        330     184

  Fragments arranged by size: 

                 731     473     415     378     364     330     302     276
                 243     219     207     184     156      95      79      46
                  44      15
 
EagI C'GGCC_G

Cuts at:      0     902    1109    1328    4557 
   Size:        902     207     219    3229

  Fragments arranged by size: 

                3229     902     219     207
 
EarI CTCTTCn'nnn_

Cuts at:      0    2002    4557 
   Size:       2002    2555
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     575    1738    4557 
   Size:        575    1163    2819

  Fragments arranged by size: 

                2819    1163     575
 
EcoICRI GAG'CTC

Cuts at:      0     402    1322    4557 
   Size:        402     920    3235

  Fragments arranged by size: 

                3235     920     402
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     103     856    1575    2219    2433    2581    3176 
   Size:         52      51     753     719     644     214     148     595

Cuts at:   3176    3426    3474    3645    4264    4297    4557 
   Size:        250      48     171     619      33     260

  Fragments arranged by size: 

                 753     719     644     619     595     260     250     214
                 171     148      52      51      48      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     889    1288    1822    2814    3492    3811    4557 
   Size:        889     399     534     992     678     319     746

  Fragments arranged by size: 

                 992     889     746     678     534     399     319
 
EcoO109I rG'GnC_Cy

Cuts at:      0     379     879     966    1074    1405    1734    1862    2062 
   Size:        379     500      87     108     331     329     128     200

Cuts at:   2062    2116    2150    2151    2250    2469    2769    2897    2906 
   Size:         54      34       1      99     219     300     128       9

Cuts at:   2906    3224    3475    3497    3681    4200    4449    4557 
   Size:        318     251      22     184     519     249     108

  Fragments arranged by size: 

                 519     500     379     331     329     318     300     251
                 249     219     200     184     128     128     108     108
                  99      87      54      34      22       9       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4188    4557 
   Size:       4188     369
 
FseI GG_CCGG'CC

Cuts at:      0    3255    4557 
   Size:       3255    1302
 
FspI TGC'GCA

Cuts at:      0       4    1876    4557 
   Size:          4    1872    2681

  Fragments arranged by size: 

                2681    1872       4
 
FspAI rTGC'GCAy

Cuts at:      0       4    1876    4557 
   Size:          4    1872    2681

  Fragments arranged by size: 

                2681    1872       4
 
HaeII r_GCGC'y

Cuts at:      0     172     348     372    1044    1095    1275    1678    2142 
   Size:        172     176      24     672      51     180     403     464

Cuts at:   2142    2428    2874    3540    3878    4557 
   Size:        286     446     666     338     679

  Fragments arranged by size: 

                 679     672     666     464     446     403     338     286
                 180     176     172      51      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     697     729    1789    1821    2118    2150    2491    2523 
   Size:        697      32    1060      32     297      32     341      32

Cuts at:   2523    2623    2655    2808    2840    3208    3240    3547    3579 
   Size:        100      32     153      32     368      32     307      32

Cuts at:   3579    4044    4076    4557 
   Size:        465      32     481

  Fragments arranged by size: 

                1060     697     481     465     368     341     307     297
                 153     100      32      32      32      32      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0     168    1271    3536    4557 
   Size:        168    1103    2265    1021

  Fragments arranged by size: 

                2265    1103    1021     168
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      92    1553    1994    2027    3002    3149    3551    4557 
   Size:         92    1461     441      33     975     147     402    1006

  Fragments arranged by size: 

                1461    1006     975     441     402     147      92      33
            
 
MscI TGG'CCA

Cuts at:      0     889    2891    3301    3802    4045    4375    4557 
   Size:        889    2002     410     501     243     330     182

  Fragments arranged by size: 

                2002     889     501     410     330     243     182
 
MslI CAynn'nnrTG

Cuts at:      0      76    1528    4557 
   Size:         76    1452    3029

  Fragments arranged by size: 

                3029    1452      76
 
MspA1I CmG'CkG

Cuts at:      0     144     672     907     929    1443    1668    1781    1899 
   Size:        144     528     235      22     514     225     113     118

Cuts at:   1899    2761    3037    3058    3259    4028    4116    4305    4488 
   Size:        862     276      21     201     769      88     189     183

Cuts at:   4488    4496    4557 
   Size:          8      61

  Fragments arranged by size: 

                 862     769     528     514     276     235     225     201
                 189     183     144     118     113      88      61      22
                  21       8
 
NaeI GCC'GGC

Cuts at:      0     518    1109    1182    3253    3321    3541    4557 
   Size:        518     591      73    2071      68     220    1016

  Fragments arranged by size: 

                2071    1016     591     518     220      73      68
 
NarI GG'CG_CC

Cuts at:      0     169    1272    3537    4557 
   Size:        169    1103    2265    1020

  Fragments arranged by size: 

                2265    1103    1020     169
 
NcoI C'CATG_G

Cuts at:      0    1386    3419    4557 
   Size:       1386    2033    1138

  Fragments arranged by size: 

                2033    1386    1138
 
NgoMIV G'CCGG_C

Cuts at:      0     516    1107    1180    3251    3319    3539    4557 
   Size:        516     591      73    2071      68     220    1018

  Fragments arranged by size: 

                2071    1018     591     516     220      73      68
 
NspI r_CATG'y

Cuts at:      0      75    1881    3894    4557 
   Size:         75    1806    2013     663

  Fragments arranged by size: 

                2013    1806     663      75
 
PasI CC'CwG_GG

Cuts at:      0     781    1756    3228    4557 
   Size:        781     975    1472    1329

  Fragments arranged by size: 

                1472    1329     975     781
 
PciI A'CATG_T

Cuts at:      0      71    4557 
   Size:         71    4486
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1671    2242    2413    3786    3828    4557 
   Size:       1671     571     171    1373      42     729

  Fragments arranged by size: 

                1671    1373     729     571     171      42
 
PfoI T'CCnGG_A

Cuts at:      0       8     104    3442    4514    4557 
   Size:          8      96    3338    1072      43

  Fragments arranged by size: 

                3338    1072      96      43       8
 
PmlI CAC'GTG

Cuts at:      0    2123    4557 
   Size:       2123    2434
 
PpuMI rG'GwC_Cy

Cuts at:      0     379     879     966    1074    1862    2469    2769    2906 
   Size:        379     500      87     108     788     607     300     137

Cuts at:   2906    3224    3475    4200    4557 
   Size:        318     251     725     357

  Fragments arranged by size: 

                 788     725     607     500     379     357     318     300
                 251     137     108      87
 
PspOMI G'GGCC_C

Cuts at:      0    1404    1929    2116    2150    2698    3173    3497    3680 
   Size:       1404     525     187      34     548     475     324     183

Cuts at:   3680    4557 
   Size:        877

  Fragments arranged by size: 

                1404     877     548     525     475     324     187     183
                  34
 
PstI C_TGCA'G

Cuts at:      0     149     619     950    1685    4557 
   Size:        149     470     331     735    2872

  Fragments arranged by size: 

                2872     735     470     331     149
 
PvuII CAG'CTG

Cuts at:      0     144    1443    1899    2761    3058    3259    4028    4116 
   Size:        144    1299     456     862     297     201     769      88

Cuts at:   4116    4305    4496    4557 
   Size:        189     191      61

  Fragments arranged by size: 

                1299     862     769     456     297     201     191     189
                 144      88      61
 
SacI G_AGCT'C

Cuts at:      0     404    1324    4557 
   Size:        404     920    3233

  Fragments arranged by size: 

                3233     920     404
 
SanDI GG'GwC_CC

Cuts at:      0     379     966    1074    1862    2906    3224    3475    4557 
   Size:        379     587     108     788    1044     318     251    1082

  Fragments arranged by size: 

                1082    1044     788     587     379     318     251     108
            
 
SexAI A'CCwGG_T

Cuts at:      0    1556    4557 
   Size:       1556    3001
 
SfcI C'TryA_G

Cuts at:      0     145     615     946    1681    2464    4557 
   Size:        145     470     331     735     783    2093

  Fragments arranged by size: 

                2093     783     735     470     331     145
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     895    2554    2563    4557 
   Size:        895    1659       9    1994

  Fragments arranged by size: 

                1994    1659     895       9
 
SfoI GGC'GCC

Cuts at:      0     170    1273    3538    4557 
   Size:        170    1103    2265    1019

  Fragments arranged by size: 

                2265    1103    1019     170
 
SmaI CCC'GGG

Cuts at:      0     683    1814    2157    2485    2774    3064    3679    3694 
   Size:        683    1131     343     328     289     290     615      15

Cuts at:   3694    3925    4557 
   Size:        231     632

  Fragments arranged by size: 

                1131     683     632     615     343     328     290     289
                 231      15
 
SmlI C'TyrA_G

Cuts at:      0     118    1189    4557 
   Size:        118    1071    3368

  Fragments arranged by size: 

                3368    1071     118
 
SpeI A'CTAG_T

Cuts at:      0    3337    4557 
   Size:       3337    1220
 
SphI G_CATG'C

Cuts at:      0    1881    4557 
   Size:       1881    2676
 
StuI AGG'CCT

Cuts at:      0     217     997    3112    4557 
   Size:        217     780    2115    1445

  Fragments arranged by size: 

                2115    1445     780     217
 
StyI C'CwwG_G

Cuts at:      0     374     725     890     917    1079    1386    1448    2146 
   Size:        374     351     165      27     162     307      62     698

Cuts at:   2146    2253    3419    4557 
   Size:        107    1166    1138

  Fragments arranged by size: 

                1166    1138     698     374     351     307     165     162
                 107      62      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1525    1696    1761    2533    2843    4557 
   Size:       1525     171      65     772     310    1714

  Fragments arranged by size: 

                1714    1525     772     310     171      65
 
TatI w'GTAC_w

Cuts at:      0     131    4557 
   Size:        131    4426
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1051    1083    2581    2613    3459    3491    3601    3633 
   Size:       1051      32    1498      32     846      32     110      32

Cuts at:   3633    3862    3894    4557 
   Size:        229      32     663

  Fragments arranged by size: 

                1498    1051     846     663     229     110      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1558    4557 
   Size:       1558    2999
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2316    2335    3829    4341    4557 
   Size:       2316      19    1494     512     216

  Fragments arranged by size: 

                2316    1494     512     216      19
 
XmaI C'CCGG_G

Cuts at:      0     681    1812    2155    2483    2772    3062    3677    3692 
   Size:        681    1131     343     328     289     290     615      15

Cuts at:   3692    3923    4557 
   Size:        231     634

  Fragments arranged by size: 

                1131     681     634     615     343     328     290     289
                 231      15
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI     FseI     FspI    FspAI    HaeII    Hin4I     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI
    PvuII     SacI    SanDI    SexAI     SfcI     SfiI     SfoI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI    ApaLI     AscI
     AseI    AsiSI     BaeI    BciVI     BclI    BfrBI    BglII     BmtI
    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I     ClaI     DraI    EcoRI    EcoRV   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI     SgfI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XbaI     XhoI     XmnI
     ZraI
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