Restriction map for pF1KA0267
 (Linear) (Six-base) MAPSORT of: KIAA0267.seq  Check: 6868  from: 1  to: 2007



KIAA0267.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     879    1052    1657    1895    2007 
   Size:        879     173     605     238     112

  Fragments arranged by size: 

                 879     605     238     173     112
 
AleI CACnn'nnGTG

Cuts at:      0    1550    2007 
   Size:       1550     457
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      88    2007 
   Size:         88    1919
 
AlwNI CAG_nnn'CTG

Cuts at:      0     688     700    2007 
   Size:        688      12    1307

  Fragments arranged by size: 

                1307     688      12
 
ApaI G_GGCC'C

Cuts at:      0     288    2007 
   Size:        288    1719
 
ApoI r'AATT_y

Cuts at:      0    1170    1338    1974    2007 
   Size:       1170     168     636      33

  Fragments arranged by size: 

                1170     636     168      33
 
AvaI C'yCGr_G

Cuts at:      0      56    2007 
   Size:         56    1951
 
BanI G'GyrC_C

Cuts at:      0      24     670    1486    1709    2007 
   Size:         24     646     816     223     298

  Fragments arranged by size: 

                 816     646     298     223      24
 
BanII G_rGCy'C

Cuts at:      0      63     288    2007 
   Size:         63     225    1719

  Fragments arranged by size: 

                1719     225      63
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     107     141    2007 
   Size:        107      34    1866

  Fragments arranged by size: 

                1866     107      34
 
BclI T'GATC_A

Cuts at:      0    1623    2007 
   Size:       1623     384
 
Bme1580I G_kGCm'C

Cuts at:      0      27     288    2007 
   Size:         27     261    1719

  Fragments arranged by size: 

                1719     261      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     123    2007 
   Size:        123    1884
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     848     947    1032    2007 
   Size:        848      99      85     975

  Fragments arranged by size: 

                 975     848      99      85
 
Bpu10I CC'TnA_GC

Cuts at:      0     391    2007 
   Size:        391    1616
 
BsaAI yAC'GTr

Cuts at:      0    1097    2007 
   Size:       1097     910
 
BsaBI GATnn'nnATC

Cuts at:      0    1766    2007 
   Size:       1766     241
 
BsaHI Gr'CG_yC

Cuts at:      0      41     104    2007 
   Size:         41      63    1903

  Fragments arranged by size: 

                1903      63      41
 
BsaWI w'CCGG_w

Cuts at:      0    1604    2007 
   Size:       1604     403
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     157     187     961     991    1238    1268    2007 
   Size:        157      30     774      30     247      30     739

  Fragments arranged by size: 

                 774     739     247     157      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82     178     205     792    2007 
   Size:         82      96      27     587    1215

  Fragments arranged by size: 

                1215     587      96      82      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     424    2007 
   Size:        280     144    1583

  Fragments arranged by size: 

                1583     280     144
 
BsmI GAATG_Cn'

Cuts at:      0     356    2007 
   Size:        356    1651
 
Bsp1286I G_dGCh'C

Cuts at:      0      27      63     288    2007 
   Size:         27      36     225    1719

  Fragments arranged by size: 

                1719     225      36      27
 
BspEI T'CCGG_A

Cuts at:      0    1604    2007 
   Size:       1604     403
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     227     478    2007 
   Size:        227     251    1529

  Fragments arranged by size: 

                1529     251     227
 
BsrDI GCAATG_nn'

Cuts at:      0     917    1385    1502    2007 
   Size:        917     468     117     505

  Fragments arranged by size: 

                 917     505     468     117
 
BsrFI r'CCGG_y

Cuts at:      0     200     304    1830    2007 
   Size:        200     104    1526     177

  Fragments arranged by size: 

                1526     200     177     104
 
BssSI C'ACGA_G

Cuts at:      0     718    2007 
   Size:        718    1289
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     916    1255    1591    2007 
   Size:        916     339     336     416

  Fragments arranged by size: 

                 916     416     339     336
 
BstBI TT'CG_AA

Cuts at:      0     519    2007 
   Size:        519    1488
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     826    2007 
   Size:        826    1181
 
BstYI r'GATC_y

Cuts at:      0     878     901    2007 
   Size:        878      23    1106

  Fragments arranged by size: 

                1106     878      23
 
BtgI C'CryG_G

Cuts at:      0      36     155    1112    2007 
   Size:         36     119     957     895

  Fragments arranged by size: 

                 957     895     119      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     874    1686    2007 
   Size:        874     812     321

  Fragments arranged by size: 

                 874     812     321
 
BtsI GCAGTG_nn'

Cuts at:      0     104    1490    2007 
   Size:        104    1386     517

  Fragments arranged by size: 

                1386     517     104
 
EaeI y'GGCC_r

Cuts at:      0    1394    1832    2007 
   Size:       1394     438     175

  Fragments arranged by size: 

                1394     438     175
 
EarI CTCTTCn'nnn_

Cuts at:      0     274    1266    2007 
   Size:        274     992     741

  Fragments arranged by size: 

                 992     741     274
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      22     155    2007 
   Size:         22     133    1852

  Fragments arranged by size: 

                1852     133      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     848     879     947    1032    1052    1657    1895    2007 
   Size:        848      31      68      85      20     605     238     112

  Fragments arranged by size: 

                 848     605     238     112      85      68      31      20
            
 
HaeII r_GCGC'y

Cuts at:      0     215    1847    2007 
   Size:        215    1632     160

  Fragments arranged by size: 

                1632     215     160
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     157     189     309     341    1983    2007 
   Size:        157      32     120      32    1642      24

  Fragments arranged by size: 

                1642     157     120      32      32      24
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     962    2007 
   Size:        439     523    1045

  Fragments arranged by size: 

                1045     523     439
 
MscI TGG'CCA

Cuts at:      0    1396    2007 
   Size:       1396     611
 
MslI CAynn'nnrTG

Cuts at:      0     859    1354    1495    1550    2007 
   Size:        859     495     141      55     457

  Fragments arranged by size: 

                 859     495     457     141      55
 
MspA1I CmG'CkG

Cuts at:      0     826    1658    1668    2007 
   Size:        826     832      10     339

  Fragments arranged by size: 

                 832     826     339      10
 
NcoI C'CATG_G

Cuts at:      0     155    2007 
   Size:        155    1852
 
NdeI CA'TA_TG

Cuts at:      0    1803    2007 
   Size:       1803     204
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     981    1917    2007 
   Size:        981     936      90

  Fragments arranged by size: 

                 981     936      90
 
PfoI T'CCnGG_A

Cuts at:      0    1009    2007 
   Size:       1009     998
 
PshAI GACnn'nnGTC

Cuts at:      0     836    2007 
   Size:        836    1171
 
PspOMI G'GGCC_C

Cuts at:      0     284    2007 
   Size:        284    1723
 
PvuII CAG'CTG

Cuts at:      0     826    2007 
   Size:        826    1181
 
SapI GCTCTTCn'nnn_

Cuts at:      0     274    1266    2007 
   Size:        274     992     741

  Fragments arranged by size: 

                 992     741     274
 
SnaBI TAC'GTA

Cuts at:      0    1097    2007 
   Size:       1097     910
 
SspI AAT'ATT

Cuts at:      0    1284    2007 
   Size:       1284     723
 
StuI AGG'CCT

Cuts at:      0     992    2007 
   Size:        992    1015
 
StyI C'CwwG_G

Cuts at:      0     155     885    1391    1849    1883    2007 
   Size:        155     730     506     458      34     124

  Fragments arranged by size: 

                 730     506     458     155     124      34
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     904    1540    2007 
   Size:        904     636     467

  Fragments arranged by size: 

                 904     636     467
 
Tth111I GACn'n_nGTC

Cuts at:      0    1997    2007 
   Size:       1997      10
 
XmnI GAAnn'nnTTC

Cuts at:      0    1869    2007 
   Size:       1869     138
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BcgI     BclI Bme1580I     BmrI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI     BsmI Bsp1286I
    BspEI    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI
    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
    PflMI     PfoI    PshAI   PspOMI    PvuII     SapI    SnaBI     SspI
     StuI     StyI    TaqII  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BsrBI    BsrGI   BssHII
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SwaI
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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