Restriction map for pF1KA0185
##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AccI        AclI        AgeI        AscI        AseI        
AsnI        AvrII       BbrPI       BclI        BglII       
BlnI        BsaAI       BsaBI       BsiEI       BsiWI       
Bsp1407I    BspDI       BsrGI       BssHII      BssSI       
Bst1107I    BstZ17I     ClaI        CpoI        DraI        
EagI        EclXI       Eco52I      EcoT22I     FbaI        
HpaI        MamI        MfeI        MluI        MunI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SalI        
SgrAI       SmaI        SnaBI       SpeI        SspBI       
SspI        SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        5437  GACGTC               GACGT^C
AccIII       1476  TCCGGA               T^CCGGA
AflII        4936  CTTAAG               C^TTAAG
Aor51HI      5507  AGCGCT               AGC^GCT
ApaI         4931  GGGCCC               GGGCC^C
AviII        2474  TGCGCA               TGC^GCA
BbeI         5194  GGCGCC               GGCGC^C
BfrI         4936  CTTAAG               C^TTAAG
BseAI        1476  TCCGGA               T^CCGGA
BspEI        1476  TCCGGA               T^CCGGA
BspLU11I     5006  ACATGT               A^CATGT
BstBI        3335  TTCGAA               TT^CGAA
BstEII       2346  GGTGACC              G^GT[ACGT]ACC
BstPI        2346  GGTGACC              G^GT[ACGT]ACC
DrdI         3123  GACTGTCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     5507  AGCGCT               AGC^GCT
EcoO65I      2346  GGTGACC              G^GT[ACGT]ACC
EcoRI        3530  GAATTC               G^AATTC
EcoRV        1337  GATATC               GAT^ATC
FseI         3441  GGCCGGCC             GGCCGG^CC
FspI         2474  TGCGCA               TGC^GCA
KasI         5194  GGCGCC               G^GCGCC
KspI         4811  CCGCGG               CCGC^GG
MroI         1476  TCCGGA               T^CCGGA
NarI         5194  GGCGCC               GG^CGCC
NdeI         1394  CATATG               CA^TATG
NspV         3335  TTCGAA               TT^CGAA
SacII        4811  CCGCGG               CCGC^GG
SapI         2208  GAAGAGC              GCTCTTC(1/4)
ScaI         1723  AGTACT               AGT^ACT
SfiI         3603  GGCCCTGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         5194  GGCGCC               GGC^GCC
SfuI         3335  TTCGAA               TT^CGAA
SphI          560  GCATGC               GCATG^C
StuI         1226  AGGCCT               AGG^CCT
XbaI         3204  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Acc65I        574  GGTACC               G^GTACC
             1437  GGTACC              
AhdI          842  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2918  GACAGGGTGTC         
Alw44I       1021  GTGCAC               G^TGCAC
             2716  GTGCAC              
ApaLI        1021  GTGCAC               G^TGCAC
             2716  GTGCAC              
Asp718I       574  GGTACC               G^GTACC
             1437  GGTACC              
AspEI         842  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2918  GACAGGGTGTC         
AvaI           26  CCCGAG               C^[CT]CG[GA]G
             3269  CCCGAG              
BcgI         1072  CGACTCTCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4541  CGAATGTGCTGC        
BciVI         580  GTATCC               GTATCC(6/5)
             3185  GTATCC              
BglI          314  GCCTCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3604  GCCCTGAGGGC         
BsaI         4280  GAGACC               GGTCTC(1/5)
             2130  GGTCTC              
BsaWI        1476  TCCGGA               [AT]^CCGG[AT]
             5144  ACCGGA              
BsoBI          26  CCCGAG               C^[CT]CG[GA]G
             3269  CCCGAG              
BspHI        1341  TCATGA               T^CATGA
             2279  TCATGA              
BsrBI        5332  GAGCGG               CCGCTC(-3/-3)
             5455  GAGCGG              
Eam1105I      842  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2918  GACAGGGTGTC         
HincII        523  GTCAAC               GT[CT]^[GA]AC
              922  GTTGAC              
HindII        523  GTCAAC               GT[CT]^[GA]AC
              922  GTTGAC              
KpnI          574  GGTACC               GGTAC^C
             1437  GGTACC              
PshAI        2401  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5031  GACCAAGGTC          
PvuII        4594  CAGCTG               CAG^CTG
             5317  CAGCTG              
RcaI         1341  TCATGA               T^CATGA
             2279  TCATGA              
SbfI         1864  CCTGCAGG             CCTGCA^GG
             4887  CCTGCAGG            
SexAI        2612  ACCTGGT              A^CC[AT]GGT
             4166  ACCTGGT             
Sse8387I     1864  CCTGCAGG             CCTGCA^GG
             4887  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        Asp700I     AspHI       AspI        AvaII       
BalI        BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PpuMI       PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmnI        
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