Restriction map for
pF1KA0185
(Linear) (Six-base) MAPSORT of: KIAA0185.seq Check: 5551 from: 1 to: 5613
KIAA0185.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 366 2044 2901 4895 5613
Size: 366 1678 857 1994 718
Fragments arranged by size:
1994 1678 857 718 366
AatII G_ACGT'C
Cuts at: 0 5441 5613
Size: 5441 172
Acc65I G'GTAC_C
Cuts at: 0 574 1437 5613
Size: 574 863 4176
Fragments arranged by size:
4176 863 574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 418 571 1177 1556 3265 3589 4136
Size: 131 287 153 606 379 1709 324 547
Cuts at: 4136 4586 4935 5173 5613
Size: 450 349 238 440
Fragments arranged by size:
1709 606 547 450 440 379 349 324
287 238 153 131
AfeI AGC'GCT
Cuts at: 0 5509 5613
Size: 5509 104
AflII C'TTAA_G
Cuts at: 0 4936 5613
Size: 4936 677
AflIII A'CryG_T
Cuts at: 0 4502 4976 5006 5613
Size: 4502 474 30 607
Fragments arranged by size:
4502 607 474 30
AhdI GACnn_n'nnGTC
Cuts at: 0 847 2923 5613
Size: 847 2076 2690
Fragments arranged by size:
2690 2076 847
AleI CACnn'nnGTG
Cuts at: 0 916 3788 5613
Size: 916 2872 1825
Fragments arranged by size:
2872 1825 916
AlwNI CAG_nnn'CTG
Cuts at: 0 714 1701 2454 2517 3124 3606 3978 4270
Size: 714 987 753 63 607 482 372 292
Cuts at: 4270 5613
Size: 1343
Fragments arranged by size:
1343 987 753 714 607 482 372 292
63
ApaI G_GGCC'C
Cuts at: 0 4935 5613
Size: 4935 678
ApaLI G'TGCA_C
Cuts at: 0 1021 2716 5613
Size: 1021 1695 2897
Fragments arranged by size:
2897 1695 1021
ApoI r'AATT_y
Cuts at: 0 2290 3530 5116 5613
Size: 2290 1240 1586 497
Fragments arranged by size:
2290 1586 1240 497
AvaI C'yCGr_G
Cuts at: 0 26 3269 5613
Size: 26 3243 2344
Fragments arranged by size:
3243 2344 26
BamHI G'GATC_C
Cuts at: 0 118 939 2154 3057 4788 5613
Size: 118 821 1215 903 1731 825
Fragments arranged by size:
1731 1215 903 825 821 118
BanI G'GyrC_C
Cuts at: 0 574 1437 1704 3223 5194 5613
Size: 574 863 267 1519 1971 419
Fragments arranged by size:
1971 1519 863 574 419 267
BanII G_rGCy'C
Cuts at: 0 1721 4669 4843 4935 5253 5599 5613
Size: 1721 2948 174 92 318 346 14
Fragments arranged by size:
2948 1721 346 318 174 92 14
BbeI G_GCGC'C
Cuts at: 0 5198 5613
Size: 5198 415
BbsI GAAGACnn'nnnn_
Cuts at: 0 599 1206 1942 3262 3301 3824 4592 5613
Size: 599 607 736 1320 39 523 768 1021
Fragments arranged by size:
1320 1021 768 736 607 599 523 39
BbvCI CC'TCA_GC
Cuts at: 0 542 2764 4271 5613
Size: 542 2222 1507 1342
Fragments arranged by size:
2222 1507 1342 542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1061 1095 4530 4564 5613
Size: 1061 34 3435 34 1049
Fragments arranged by size:
3435 1061 1049 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 591 3196 5613
Size: 591 2605 2417
Fragments arranged by size:
2605 2417 591
BglI GCCn_nnn'nGGC
Cuts at: 0 320 3610 5613
Size: 320 3290 2003
Fragments arranged by size:
3290 2003 320
BlpI GC'TnA_GC
Cuts at: 0 1186 4837 5362 5613
Size: 1186 3651 525 251
Fragments arranged by size:
3651 1186 525 251
Bme1580I G_kGCm'C
Cuts at: 0 1025 1375 1709 2609 2720 3226 4935 5613
Size: 1025 350 334 900 111 506 1709 678
Fragments arranged by size:
1709 1025 900 678 506 350 334 111
BmgBI CAC'GTC
Cuts at: 0 4212 4503 5613
Size: 4212 291 1110
Fragments arranged by size:
4212 1110 291
BmrI ACTGGGnnnn_n'
Cuts at: 0 4208 5613
Size: 4208 1405
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3243 3963 5613
Size: 3243 720 1650
Fragments arranged by size:
3243 1650 720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1972 2032 2476 3394 5020 5275 5596
Size: 301 1671 60 444 918 1626 255 321
Cuts at: 5596 5613
Size: 17
Fragments arranged by size:
1671 1626 918 444 321 301 255 60
17
Bpu10I CC'TnA_GC
Cuts at: 0 542 857 1027 2764 4271 5613
Size: 542 315 170 1737 1507 1342
Fragments arranged by size:
1737 1507 1342 542 315 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2923 3517 3700 5329 5613
Size: 2923 594 183 1629 284
Fragments arranged by size:
2923 1629 594 284 183
BsaI GGTCTCn'nnnn_
Cuts at: 0 2136 4274 5613
Size: 2136 2138 1339
Fragments arranged by size:
2138 2136 1339
BsaHI Gr'CG_yC
Cuts at: 0 1059 3808 5195 5438 5613
Size: 1059 2749 1387 243 175
Fragments arranged by size:
2749 1387 1059 243 175
BsaWI w'CCGG_w
Cuts at: 0 1476 5144 5613
Size: 1476 3668 469
Fragments arranged by size:
3668 1476 469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 740 770 1471 1501 3082 3112 3784 3814
Size: 740 30 701 30 1581 30 672 30
Cuts at: 3814 4993 5023 5613
Size: 1179 30 590
Fragments arranged by size:
1581 1179 740 701 672 590 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 44 764 2377 2425 2428 2473 4267 4288
Size: 44 720 1613 48 3 45 1794 21
Cuts at: 4288 4357 4495 4540 4693 4789 4972 5226 5613
Size: 69 138 45 153 96 183 254 387
Fragments arranged by size:
1794 1613 720 387 254 183 153 138
96 69 48 45 45 44 21 3
BseYI C'CCAG_C
Cuts at: 0 1138 4070 4357 5303 5319 5613
Size: 1138 2932 287 946 16 294
Fragments arranged by size:
2932 1138 946 294 287 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1714 2578 2815 5074 5572 5613
Size: 1714 864 237 2259 498 41
Fragments arranged by size:
2259 1714 864 498 237 41
BsiHKAI G_wGCw'C
Cuts at: 0 1025 1721 2720 4669 4843 5368 5599 5613
Size: 1025 696 999 1949 174 525 231 14
Fragments arranged by size:
1949 1025 999 696 525 231 174 14
BsmI GAATG_Cn'
Cuts at: 0 254 443 2192 5613
Size: 254 189 1749 3421
Fragments arranged by size:
3421 1749 254 189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2830 3747 3799 3907 4531 5613
Size: 2830 917 52 108 624 1082
Fragments arranged by size:
2830 1082 917 624 108 52
Bsp1286I G_dGCh'C
Cuts at: 0 1025 1375 1709 1721 2609 2720 3226 4669
Size: 1025 350 334 12 888 111 506 1443
Cuts at: 4669 4843 4935 5253 5368 5599 5613
Size: 174 92 318 115 231 14
Fragments arranged by size:
1443 1025 888 506 350 334 318 231
174 115 111 92 14 12
BspEI T'CCGG_A
Cuts at: 0 1476 5613
Size: 1476 4137
BspHI T'CATG_A
Cuts at: 0 1341 2279 5613
Size: 1341 938 3334
Fragments arranged by size:
3334 1341 938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 366 1609 2044 2901 4895 5613
Size: 366 1243 435 857 1994 718
Fragments arranged by size:
1994 1243 857 718 435 366
BsrBI CCG'CTC
Cuts at: 0 5334 5457 5613
Size: 5334 123 156
Fragments arranged by size:
5334 156 123
BsrDI GCAATG_nn'
Cuts at: 0 794 2819 5288 5613
Size: 794 2025 2469 325
Fragments arranged by size:
2469 2025 794 325
BsrFI r'CCGG_y
Cuts at: 0 2988 3442 4468 5613
Size: 2988 454 1026 1145
Fragments arranged by size:
2988 1145 1026 454
BstAPI GCAn_nnn'nTGC
Cuts at: 0 995 2376 2472 5613
Size: 995 1381 96 3141
Fragments arranged by size:
3141 1381 995 96
BstBI TT'CG_AA
Cuts at: 0 3336 5613
Size: 3336 2277
BstEII G'GTnAC_C
Cuts at: 0 2346 5613
Size: 2346 3267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1090 1452 5128 5574 5613
Size: 1090 362 3676 446 39
Fragments arranged by size:
3676 1090 446 362 39
BstYI r'GATC_y
Cuts at: 0 44 118 939 2154 3057 3933 4788 5613
Size: 44 74 821 1215 903 876 855 825
Fragments arranged by size:
1215 903 876 855 825 821 74 44
Bsu36I CC'TnA_GG
Cuts at: 0 1223 2247 3607 5613
Size: 1223 1024 1360 2006
Fragments arranged by size:
2006 1360 1223 1024
BtgI C'CryG_G
Cuts at: 0 597 3416 3698 4811 4895 4919 5613
Size: 597 2819 282 1113 84 24 694
Fragments arranged by size:
2819 1113 694 597 282 84 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2623 5613
Size: 2623 2990
BtsI GCAGTG_nn'
Cuts at: 0 646 983 1103 1367 2386 3263 3386 3860
Size: 646 337 120 264 1019 877 123 474
Cuts at: 3860 5613
Size: 1753
Fragments arranged by size:
1753 1019 877 646 474 337 264 123
120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3603 3638 5613
Size: 3603 35 1975
Fragments arranged by size:
3603 1975 35
DraIII CAC_nnn'GTG
Cuts at: 0 4012 4014 5238 5613
Size: 4012 2 1224 375
Fragments arranged by size:
4012 1224 375 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 3129 5613
Size: 3129 2484
EaeI y'GGCC_r
Cuts at: 0 791 1764 2148 2519 3361 3381 3444 3597
Size: 791 973 384 371 842 20 63 153
Cuts at: 3597 3695 5613
Size: 98 1918
Fragments arranged by size:
1918 973 842 791 384 371 153 98
63 20
EarI CTCTTCn'nnn_
Cuts at: 0 107 484 2203 4529 5613
Size: 107 377 1719 2326 1084
Fragments arranged by size:
2326 1719 1084 377 107
EcoICRI GAG'CTC
Cuts at: 0 1719 4667 4841 5597 5613
Size: 1719 2948 174 756 16
Fragments arranged by size:
2948 1719 756 174 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 301 418 571 1177 1556 1972 2032
Size: 131 170 117 153 606 379 416 60
Cuts at: 2032 2476 3265 3394 3589 4136 4586 4935 5020
Size: 444 789 129 195 547 450 349 85
Cuts at: 5020 5173 5275 5596 5613
Size: 153 102 321 17
Fragments arranged by size:
789 606 547 450 444 416 379 349
321 195 170 153 153 131 129 117
102 85 60 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 2731 4477 5032 5613
Size: 241 2490 1746 555 581
Fragments arranged by size:
2490 1746 581 555 241
EcoO109I rG'GnC_Cy
Cuts at: 0 402 546 983 1431 3603 3667 4140 4931
Size: 402 144 437 448 2172 64 473 791
Cuts at: 4931 4932 5571 5613
Size: 1 639 42
Fragments arranged by size:
2172 791 639 473 448 437 402 144
64 42 1
EcoRI G'AATT_C
Cuts at: 0 3530 5613
Size: 3530 2083
EcoRV GAT'ATC
Cuts at: 0 1339 5613
Size: 1339 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1230 2142 3663 3991 4255 5517 5613
Size: 1230 912 1521 328 264 1262 96
Fragments arranged by size:
1521 1262 1230 912 328 264 96
FseI GG_CCGG'CC
Cuts at: 0 3446 5613
Size: 3446 2167
FspI TGC'GCA
Cuts at: 0 2476 5613
Size: 2476 3137
HaeII r_GCGC'y
Cuts at: 0 4585 4783 5198 5511 5613
Size: 4585 198 415 313 102
Fragments arranged by size:
4585 415 313 198 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 833 865 1049 1081 1458 1490 2644 2676
Size: 833 32 184 32 377 32 1154 32
Cuts at: 2676 2909 2941 3784 3816 3836 3868 5377 5409
Size: 233 32 843 32 20 32 1509 32
Cuts at: 5409 5613
Size: 204
Fragments arranged by size:
1509 1154 843 833 377 233 204 184
32 32 32 32 32 32 32 32
20
HincII GTy'rAC
Cuts at: 0 525 924 5613
Size: 525 399 4689
Fragments arranged by size:
4689 525 399
HindIII A'AGCT_T
Cuts at: 0 18 62 3676 5613
Size: 18 44 3614 1937
Fragments arranged by size:
3614 1937 44 18
KasI G'GCGC_C
Cuts at: 0 5194 5613
Size: 5194 419
KpnI G_GTAC'C
Cuts at: 0 578 1441 5613
Size: 578 863 4172
Fragments arranged by size:
4172 863 578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1988 2378 2549 2690 4715 5613
Size: 1988 390 171 141 2025 898
Fragments arranged by size:
2025 1988 898 390 171 141
MscI TGG'CCA
Cuts at: 0 1766 2150 3383 3599 3697 5613
Size: 1766 384 1233 216 98 1916
Fragments arranged by size:
1916 1766 1233 384 216 98
MslI CAynn'nnrTG
Cuts at: 0 916 1465 3788 5282 5613
Size: 916 549 2323 1494 331
Fragments arranged by size:
2323 1494 916 549 331
MspA1I CmG'CkG
Cuts at: 0 2373 4596 4813 5319 5613
Size: 2373 2223 217 506 294
Fragments arranged by size:
2373 2223 506 294 217
NaeI GCC'GGC
Cuts at: 0 2990 3444 4470 5613
Size: 2990 454 1026 1143
Fragments arranged by size:
2990 1143 1026 454
NarI GG'CG_CC
Cuts at: 0 5195 5613
Size: 5195 418
NcoI C'CATG_G
Cuts at: 0 597 3416 3698 5613
Size: 597 2819 282 1915
Fragments arranged by size:
2819 1915 597 282
NdeI CA'TA_TG
Cuts at: 0 1395 5613
Size: 1395 4218
NgoMIV G'CCGG_C
Cuts at: 0 2988 3442 4468 5613
Size: 2988 454 1026 1145
Fragments arranged by size:
2988 1145 1026 454
NspI r_CATG'y
Cuts at: 0 564 5010 5281 5613
Size: 564 4446 271 332
Fragments arranged by size:
4446 564 332 271
PciI A'CATG_T
Cuts at: 0 5006 5613
Size: 5006 607
PflMI CCAn_nnn'nTGG
Cuts at: 0 2517 2739 4862 5613
Size: 2517 222 2123 751
Fragments arranged by size:
2517 2123 751 222
PfoI T'CCnGG_A
Cuts at: 0 2181 3344 3953 5120 5441 5613
Size: 2181 1163 609 1167 321 172
Fragments arranged by size:
2181 1167 1163 609 321 172
PpuMI rG'GwC_Cy
Cuts at: 0 402 3667 4140 5613
Size: 402 3265 473 1473
Fragments arranged by size:
3265 1473 473 402
PshAI GACnn'nnGTC
Cuts at: 0 2405 5035 5613
Size: 2405 2630 578
Fragments arranged by size:
2630 2405 578
PsiI TTA'TAA
Cuts at: 0 4100 5613
Size: 4100 1513
PspOMI G'GGCC_C
Cuts at: 0 4931 5613
Size: 4931 682
PstI C_TGCA'G
Cuts at: 0 1631 1869 2468 3867 4595 4871 4892 5226
Size: 1631 238 599 1399 728 276 21 334
Cuts at: 5226 5246 5613
Size: 20 367
Fragments arranged by size:
1631 1399 728 599 367 334 276 238
21 20
PvuII CAG'CTG
Cuts at: 0 4596 5319 5613
Size: 4596 723 294
Fragments arranged by size:
4596 723 294
SacI G_AGCT'C
Cuts at: 0 1721 4669 4843 5599 5613
Size: 1721 2948 174 756 14
Fragments arranged by size:
2948 1721 756 174 14
SacII CC_GC'GG
Cuts at: 0 4814 5613
Size: 4814 799
SapI GCTCTTCn'nnn_
Cuts at: 0 2203 5613
Size: 2203 3410
SbfI CC_TGCA'GG
Cuts at: 0 1869 4892 5613
Size: 1869 3023 721
Fragments arranged by size:
3023 1869 721
ScaI AGT'ACT
Cuts at: 0 1725 5613
Size: 1725 3888
SexAI A'CCwGG_T
Cuts at: 0 2612 4166 5613
Size: 2612 1554 1447
Fragments arranged by size:
2612 1554 1447
SfcI C'TryA_G
Cuts at: 0 1048 1269 1627 1865 2464 3071 3863 4591
Size: 1048 221 358 238 599 607 792 728
Cuts at: 4591 4867 4888 5222 5242 5613
Size: 276 21 334 20 371
Fragments arranged by size:
1048 792 728 607 599 371 358 334
276 238 221 21 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3610 5613
Size: 3610 2003
SfoI GGC'GCC
Cuts at: 0 5196 5613
Size: 5196 417
SmlI C'TyrA_G
Cuts at: 0 2938 3496 3679 4936 5308 5613
Size: 2938 558 183 1257 372 305
Fragments arranged by size:
2938 1257 558 372 305 183
SphI G_CATG'C
Cuts at: 0 564 5613
Size: 564 5049
StuI AGG'CCT
Cuts at: 0 1228 5613
Size: 1228 4385
StyI C'CwwG_G
Cuts at: 0 597 1089 2126 2828 3416 3698 4026 4226
Size: 597 492 1037 702 588 282 328 200
Cuts at: 4226 4553 5033 5567 5613
Size: 327 480 534 46
Fragments arranged by size:
1037 702 597 588 534 492 480 328
327 282 200 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4841 4971 5385 5613
Size: 4841 130 414 228
Fragments arranged by size:
4841 414 228 130
TatI w'GTAC_w
Cuts at: 0 665 1723 4505 4871 5057 5613
Size: 665 1058 2782 366 186 556
Fragments arranged by size:
2782 1058 665 556 366 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3082 3114 4066 4098 5613
Size: 3082 32 952 32 1515
Fragments arranged by size:
3082 1515 952 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1874 2614 3112 3823 3882 5613
Size: 1874 740 498 711 59 1731
Fragments arranged by size:
1874 1731 740 711 498 59
XbaI T'CTAG_A
Cuts at: 0 3204 5613
Size: 3204 2409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1947 2826 3108 3705 5613
Size: 1947 879 282 597 1908
Fragments arranged by size:
1947 1908 879 597 282
XmnI GAAnn'nnTTC
Cuts at: 0 64 1213 3298 5158 5497 5613
Size: 64 1149 2085 1860 339 116
Fragments arranged by size:
2085 1860 1149 339 116 64
ZraI GAC'GTC
Cuts at: 0 5439 5613
Size: 5439 174
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PpuMI PshAI PsiI PspOMI PstI PvuII
SacI SacII SapI SbfI ScaI SexAI SfcI SfiI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AgeI AlfI AloI AscI AseI AsiSI
AvrII BaeI BclI BfrBI BglII BmtI BsaAI BsaBI
BsiEI BsiWI BsrGI BssHII BssSI BstZ17I ClaI DraI
EagI EciI FspAI HpaI MfeI MluI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PspXI
PsrI PvuI RsrII SalI SanDI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI XhoI XmaI