Restriction map for pF1KB9887
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflIII      
AgeI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BamHI       BciVI       BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsiWI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        EcoO65I     EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MflI        
MluI        MslI        MunI        NcoI        NdeI        
NheI        NotI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Tth111I     
XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI         1123  GAATTC               [GA]^AATT[CT]
AflII         771  CTTAAG               C^TTAAG
AhdI         1069  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1423  GATCC                GGATC(4/5)
AocI          268  CCTGAGG              CC^T[ACGT]AGG
ApaI          546  GGGCCC               GGGCC^C
ApoI         1123  GAATTC               [GA]^AATT[CT]
Asp700I      1666  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1069  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         2163  GGCGCC               GGCGC^C
BbsI         1372  GTCTTC               GAAGAC(2/6)
BcgI         1352  CGAAAACGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          743  TGATCA               T^GATCA
BfrI          771  CTTAAG               C^TTAAG
BpuAI        1372  GTCTTC               GAAGAC(2/6)
BsmI         1524  GAATGC               GAATGC(1/-1)
BsrDI        2150  GCAATG               GCAATG(2/0)
BstAPI       1818  GCAGTACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2195  CCAAGAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        268  CCTGAGG              CC^T[ACGT]AGG
DraIII         25  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1069  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        268  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1123  GAATTC               G^AATTC
FbaI          743  TGATCA               T^GATCA
KasI         2163  GGCGCC               G^GCGCC
MseI          772  TTAA                 T^TAA
NaeI         1330  GCCGGC               GCC^GGC
NarI         2163  GGCGCC               GG^CGCC
NgoMIV       1330  GCCGGC               G^CCGGC
NruI          444  TCGCGA               TCG^CGA
SapI          304  GCTCTTC              GCTCTTC(1/4)
SfoI         2163  GGCGCC               GGC^GCC
SmaI          565  CCCGGG               CCC^GGG
SspI           86  AATATT               AAT^ATT
Tru9I         772  TTAA                 T^TAA
XbaI          957  TCTAGA               T^CTAGA
XmaCI         565  CCCGGG               C^CCGGG
XmaI          565  CCCGGG               C^CCGGG
XmnI         1666  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI         1542  GTAC                 GT^AC
             1821  GTAC                
AlwNI        1819  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1849  CAGCGGCTG           
Aor51HI       277  AGCGCT               AGC^GCT
              290  AGCGCT              
BbrPI         181  CACGTG               CAC^GTG
             1886  CACGTG              
BfaI           79  CTAG                 C^TAG
              958  CTAG                
BsaAI         181  CACGTG               [CT]AC^GT[GA]
             1886  CACGTG              
BsaI          420  GAGACC               GGTCTC(1/5)
              434  GGTCTC              
BsmBI         686  CGTCTC               CGTCTC(1/5)
             1988  GAGACG              
BspMI        1823  ACCTGC               ACCTGC(4/8)
               42  GCAGGT              
BsrFI        1330  GCCGGC               [GA]^CCGG[CT]
             1536  GCCGGT              
BssSI         232  CTCGTG               CACGAG(-5/-1)
             1259  CTCGTG              
Cfr10I       1330  GCCGGC               [GA]^CCGG[CT]
             1536  GCCGGT              
DsaI         2033  CCGCGG               C^C[GA][CT]GG
             2168  CCGCGG              
Eco47III      277  AGCGCT               AGC^GCT
              290  AGCGCT              
EcoNI        1089  CCTCTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1392  CCTCTCAGAGG         
KspI         2033  CCGCGG               CCGC^GG
             2168  CCGCGG              
MaeI           79  CTAG                 C^TAG
              958  CTAG                
MaeIII        908  GTCAC                ^GT[ACGT]AC
              932  GTCAC               
PflMI         177  CCAGCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              973  CCAGGAAATGG         
PmaCI         181  CACGTG               CAC^GTG
             1886  CACGTG              
PmlI          181  CACGTG               CAC^GTG
             1886  CACGTG              
PvuII         485  CAGCTG               CAG^CTG
             1696  CAGCTG              
RsaI         1542  GTAC                 GT^AC
             1821  GTAC                
SacI         1083  GAGCTC               GAGCT^C
             1381  GAGCTC              
SacII        2033  CCGCGG               CCGC^GG
             2168  CCGCGG              
SmlI          771  CTTAAG               C^T[CT][GA]AG
             1037  CTCAAG              
Tsp45I        908  GTCAC                ^GT[GC]AC
              932  GTCAC               
Van91I        177  CCAGCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              973  CCAGGAAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AluI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvCI       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspEI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MboI        MboII       MluNI       MnlI        
MroI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StuI        
StyI        TaqI        TfiI        TseI        Tsp509I     
TspRI       TthHB8I     
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