Restriction map for pF1KB9887
 (Linear) (Six-base) MAPSORT of: KIBB9887.seq  Check: 6050  from: 1  to: 2259



KIBB9887.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      33    2259 
   Size:         33    2226
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    2259 
   Size:        292    1967
 
AfeI AGC'GCT

Cuts at:      0     279     292    2259 
   Size:        279      13    1967

  Fragments arranged by size: 

                1967     279      13
 
AflII C'TTAA_G

Cuts at:      0     771    2259 
   Size:        771    1488
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1074    2259 
   Size:       1074    1185
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1824    1854    2259 
   Size:       1824      30     405

  Fragments arranged by size: 

                1824     405      30
 
ApaI G_GGCC'C

Cuts at:      0     550    2259 
   Size:        550    1709
 
ApoI r'AATT_y

Cuts at:      0    1123    2259 
   Size:       1123    1136
 
AvaI C'yCGr_G

Cuts at:      0     429     541     565    1000    1545    2259 
   Size:        429     112      24     435     545     714

  Fragments arranged by size: 

                 714     545     435     429     112      24
 
BanI G'GyrC_C

Cuts at:      0    1359    1575    2163    2259 
   Size:       1359     216     588      96

  Fragments arranged by size: 

                1359     588     216      96
 
BanII G_rGCy'C

Cuts at:      0     364     452     550     557    1087    1385    1552    2259 
   Size:        364      88      98       7     530     298     167     707

  Fragments arranged by size: 

                 707     530     364     298     167      98      88       7
            
 
BbeI G_GCGC'C

Cuts at:      0    2167    2259 
   Size:       2167      92
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1365    2259 
   Size:       1365     894
 
BbvCI CC'TCA_GC

Cuts at:      0     923    1118    1630    2259 
   Size:        923     195     512     629

  Fragments arranged by size: 

                 923     629     512     195
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1341    1375    2259 
   Size:       1341      34     884

  Fragments arranged by size: 

                1341     884      34
 
BclI T'GATC_A

Cuts at:      0     743    2259 
   Size:        743    1516
 
Bme1580I G_kGCm'C

Cuts at:      0     472     550     680     717    1258    1364    1602    2259 
   Size:        472      78     130      37     541     106     238     657

  Fragments arranged by size: 

                 657     541     472     238     130     106      78      37
            
 
BmgBI CAC'GTC

Cuts at:      0    1638    2259 
   Size:       1638     621
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     947    1665    2259 
   Size:        947     718     594

  Fragments arranged by size: 

                 947     718     594
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     383    2259 
   Size:        383    1876
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     884    1536    1555    2040    2259 
   Size:        884     652      19     485     219

  Fragments arranged by size: 

                 884     652     485     219      19
 
Bpu10I CC'TnA_GC

Cuts at:      0     377     551     602     923    1118    1630    2140    2259 
   Size:        377     174      51     321     195     512     510     119

  Fragments arranged by size: 

                 512     510     377     321     195     174     119      51
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1022    2259 
   Size:       1022    1237
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     414     440    2259 
   Size:        414      26    1819

  Fragments arranged by size: 

                1819     414      26
 
BsaAI yAC'GTr

Cuts at:      0     183    1888    2259 
   Size:        183    1705     371

  Fragments arranged by size: 

                1705     371     183
 
BsaHI Gr'CG_yC

Cuts at:      0    1625    1838    1948    2164    2259 
   Size:       1625     213     110     216      95

  Fragments arranged by size: 

                1625     216     213     110      95
 
BsaWI w'CCGG_w

Cuts at:      0     533    1620    1833    2259 
   Size:        533    1087     213     426

  Fragments arranged by size: 

                1087     533     426     213
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1534    1564    2038    2068    2259 
   Size:       1534      30     474      30     191

  Fragments arranged by size: 

                1534     474     191      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     286     907    1018    1117    2259 
   Size:        286     621     111      99    1142

  Fragments arranged by size: 

                1142     621     286     111      99
 
BseYI C'CCAG_C

Cuts at:      0     483    1363    2259 
   Size:        483     880     896

  Fragments arranged by size: 

                 896     880     483
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7    1199    1868    2259 
   Size:          7    1192     669     391

  Fragments arranged by size: 

                1192     669     391       7
 
BsiEI CG_ry'CG

Cuts at:      0     498     966    1029    1331    2259 
   Size:        498     468      63     302     928

  Fragments arranged by size: 

                 928     498     468     302      63
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     383    1087    1385    2159    2259 
   Size:        233     150     704     298     774     100

  Fragments arranged by size: 

                 774     704     298     233     150     100
 
BsmI GAATG_Cn'

Cuts at:      0    1530    2259 
   Size:       1530     729
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     692    1982    2259 
   Size:        692    1290     277

  Fragments arranged by size: 

                1290     692     277
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     364     383     452     472     550     557     680 
   Size:        233     131      19      69      20      78       7     123

Cuts at:    680     717    1087    1258    1364    1385    1552    1602    2159 
   Size:         37     370     171     106      21     167      50     557

Cuts at:   2159    2259 
   Size:        100

  Fragments arranged by size: 

                 557     370     233     171     167     131     123     106
                 100      78      69      50      37      21      20      19
                   7
 
BspEI T'CCGG_A

Cuts at:      0     533    1620    1833    2259 
   Size:        533    1087     213     426

  Fragments arranged by size: 

                1087     533     426     213
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      33    1832    2259 
   Size:         33    1799     427

  Fragments arranged by size: 

                1799     427      33
 
BsrDI GCAATG_nn'

Cuts at:      0    2157    2259 
   Size:       2157     102
 
BsrFI r'CCGG_y

Cuts at:      0    1330    1536    2259 
   Size:       1330     206     723

  Fragments arranged by size: 

                1330     723     206
 
BssSI C'ACGA_G

Cuts at:      0     232    1259    2259 
   Size:        232    1027    1000

  Fragments arranged by size: 

                1027    1000     232
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1824    2259 
   Size:       1824     435
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2202    2259 
   Size:       2202      57
 
Bsu36I CC'TnA_GG

Cuts at:      0     269    2259 
   Size:        269    1990
 
BtgI C'CryG_G

Cuts at:      0    2033    2168    2259 
   Size:       2033     135      91

  Fragments arranged by size: 

                2033     135      91
 
BtsI GCAGTG_nn'

Cuts at:      0      35    1211    1600    2259 
   Size:         35    1176     389     659

  Fragments arranged by size: 

                1176     659     389      35
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1200    1235    2259 
   Size:       1200      35    1024

  Fragments arranged by size: 

                1200    1024      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      30    2259 
   Size:         30    2229
 
EaeI y'GGCC_r

Cuts at:      0      53     495     870     963    1328    1940    2171    2216 
   Size:         53     442     375      93     365     612     231      45

Cuts at:   2216    2259 
   Size:         43

  Fragments arranged by size: 

                 612     442     375     365     231      93      53      45
                  43
 
EagI C'GGCC_G

Cuts at:      0     495     963    1328    2259 
   Size:        495     468     365     931

  Fragments arranged by size: 

                 931     495     468     365
 
EarI CTCTTCn'nnn_

Cuts at:      0     311     576     910     940    1482    2259 
   Size:        311     265     334      30     542     777

  Fragments arranged by size: 

                 777     542     334     311     265      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      93     148    1332    2259 
   Size:         93      55    1184     927

  Fragments arranged by size: 

                1184     927      93      55
 
EcoICRI GAG'CTC

Cuts at:      0    1085    1383    2259 
   Size:       1085     298     876

  Fragments arranged by size: 

                1085     876     298
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     884    1536    1555    2040    2259 
   Size:        292     592     652      19     485     219

  Fragments arranged by size: 

                 652     592     485     292     219      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1093    1396    2259 
   Size:       1093     303     863

  Fragments arranged by size: 

                1093     863     303
 
EcoO109I rG'GnC_Cy

Cuts at:      0     264     546     547     764     969    1389    1647    2259 
   Size:        264     282       1     217     205     420     258     612

  Fragments arranged by size: 

                 612     420     282     264     258     217     205       1
            
 
EcoRI G'AATT_C

Cuts at:      0    1123    2259 
   Size:       1123    1136
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1033    2259 
   Size:       1033    1226
 
HaeII r_GCGC'y

Cuts at:      0     165     281     294    1337    2167    2259 
   Size:        165     116      13    1043     830      92

  Fragments arranged by size: 

                1043     830     165     116      92      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     715     747     939     971    1060    1092    1426    1458 
   Size:        715      32     192      32      89      32     334      32

Cuts at:   1458    1677    1709    2259 
   Size:        219      32     550

  Fragments arranged by size: 

                 715     550     334     219     192      89      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0    2163    2259 
   Size:       2163      96
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1046    2259 
   Size:        538     508    1213

  Fragments arranged by size: 

                1213     538     508
 
MscI TGG'CCA

Cuts at:      0      55    1942    2218    2259 
   Size:         55    1887     276      41

  Fragments arranged by size: 

                1887     276      55      41
 
MspA1I CmG'CkG

Cuts at:      0     487     559     869    1698    1851    2035    2072    2170 
   Size:        487      72     310     829     153     184      37      98

Cuts at:   2170    2211    2259 
   Size:         41      48

  Fragments arranged by size: 

                 829     487     310     184     153      98      72      48
                  41      37
 
NaeI GCC'GGC

Cuts at:      0    1332    2259 
   Size:       1332     927
 
NarI GG'CG_CC

Cuts at:      0    2164    2259 
   Size:       2164      95
 
NgoMIV G'CCGG_C

Cuts at:      0    1330    2259 
   Size:       1330     929
 
NruI TCG'CGA

Cuts at:      0     446    2259 
   Size:        446    1813
 
PasI CC'CwG_GG

Cuts at:      0     645     717    2259 
   Size:        645      72    1542

  Fragments arranged by size: 

                1542     645      72
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     183     979    2259 
   Size:        183     796    1280

  Fragments arranged by size: 

                1280     796     183
 
PfoI T'CCnGG_A

Cuts at:      0    1298    1490    1769    2259 
   Size:       1298     192     279     490

  Fragments arranged by size: 

                1298     490     279     192
 
PmlI CAC'GTG

Cuts at:      0     183    1888    2259 
   Size:        183    1705     371

  Fragments arranged by size: 

                1705     371     183
 
PpuMI rG'GwC_Cy

Cuts at:      0     264     969    1389    1647    2259 
   Size:        264     705     420     258     612

  Fragments arranged by size: 

                 705     612     420     264     258
 
PspOMI G'GGCC_C

Cuts at:      0     546    2259 
   Size:        546    1713
 
PstI C_TGCA'G

Cuts at:      0      44    1325    1986    2259 
   Size:         44    1281     661     273

  Fragments arranged by size: 

                1281     661     273      44
 
PvuII CAG'CTG

Cuts at:      0     487    1698    2259 
   Size:        487    1211     561

  Fragments arranged by size: 

                1211     561     487
 
SacI G_AGCT'C

Cuts at:      0    1087    1385    2259 
   Size:       1087     298     874

  Fragments arranged by size: 

                1087     874     298
 
SacII CC_GC'GG

Cuts at:      0    2036    2171    2259 
   Size:       2036     135      88

  Fragments arranged by size: 

                2036     135      88
 
SanDI GG'GwC_CC

Cuts at:      0     969    2259 
   Size:        969    1290
 
SapI GCTCTTCn'nnn_

Cuts at:      0     311    2259 
   Size:        311    1948
 
SfcI C'TryA_G

Cuts at:      0      40    1321    1982    2259 
   Size:         40    1281     661     277

  Fragments arranged by size: 

                1281     661     277      40
 
SfoI GGC'GCC

Cuts at:      0    2165    2259 
   Size:       2165      94
 
SmaI CCC'GGG

Cuts at:      0     567    2259 
   Size:        567    1692
 
SmlI C'TyrA_G

Cuts at:      0     771    1037    2259 
   Size:        771     266    1222

  Fragments arranged by size: 

                1222     771     266
 
SspI AAT'ATT

Cuts at:      0      88    2259 
   Size:         88    2171
 
StuI AGG'CCT

Cuts at:      0     325     921    1402    1557    2259 
   Size:        325     596     481     155     702

  Fragments arranged by size: 

                 702     596     481     325     155
 
StyI C'CwwG_G

Cuts at:      0     787     887    1250    2219    2259 
   Size:        787     100     363     969      40

  Fragments arranged by size: 

                 969     787     363     100      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     254    2259 
   Size:        254    2005
 
TatI w'GTAC_w

Cuts at:      0    1541    2259 
   Size:       1541     718
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1472    1504    1505    1537    2038    2070    2259 
   Size:       1472      32       1      32     501      32     189

  Fragments arranged by size: 

                1472     501     189      32      32      32       1
 
XbaI T'CTAG_A

Cuts at:      0     957    2259 
   Size:        957    1302
 
XmaI C'CCGG_G

Cuts at:      0     565    2259 
   Size:        565    1694
 
XmnI GAAnn'nnTTC

Cuts at:      0    1670    2259 
   Size:       1670     589
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AflII     AhdI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI
    BssSI   BstAPI    BstXI   Bsu36I     BtgI     BtsI    CspCI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I     KasI     MmeI     MscI   MspA1I
     NaeI     NarI   NgoMIV     NruI     PasI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI
     SfcI     SfoI     SmaI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI    BfrBI     BglI    BglII     BlpI     BmtI    BsaBI    BsiWI
    BspHI    BsrBI    BsrGI   BssHII    BstBI   BstEII    BstYI  BstZ17I
    BtgZI     ClaI     DraI     DrdI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MslI     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PacI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XcmI     XhoI     ZraI
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