Restriction map for pF1KA0103
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# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AfaI        AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       BstAPI      BstEII      BstPI       
BstUI       BstXI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        TfiI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI          597  GTATAC               GT^[AC][GT]AC
AflII         844  CTTAAG               C^TTAAG
AflIII        860  ACATGT               A^C[GA][CT]GT
BfrI          844  CTTAAG               C^TTAAG
BspLU11I      860  ACATGT               A^CATGT
BspMI         811  GCAGGT               ACCTGC(4/8)
BsrDI         872  CATTGC               GCAATG(2/0)
BsrI          885  CCAGT                ACTGG(1/-1)
Bst1107I      597  GTATAC               GTA^TAC
BstBI         389  TTCGAA               TT^CGAA
BstF5I        204  GGATG                GGATG(2/0)
BstYI         348  AGATCC               [GA]^GATC[CT]
BstZ17I       597  GTATAC               GTA^TAC
DsaI          296  CCATGG               C^C[GA][CT]GG
FokI          204  GGATG                GGATG(9/13)
HaeIII        417  GGCC                 GG^CC
HinfI         263  GAGTC                G^A[ACGT]TC
MflI          348  AGATCC               [GA]^GATC[CT]
MslI          505  CATGACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          296  CCATGG               C^CATGG
NspI          860  ACATGT               [GA]CATG^[CT]
NspV          389  TTCGAA               TT^CGAA
PacI          118  TTAATTAA             TTAAT^TAA
PleI          263  GAGTC                GAGTC(4/5)
PshAI         193  GACTATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI         313  GATGC                GCATC(5/9)
SfuI          389  TTCGAA               TT^CGAA
SspI          836  AATATT               AAT^ATT
TspRI         360  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         348  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AlwI          349  GATCC                GGATC(4/5)
              158  GGATC               
AseI          117  ATTAAT               AT^TAAT
              121  ATTAAT              
AsnI          117  ATTAAT               AT^TAAT
              121  ATTAAT              
BsaI          458  GAGACC               GGTCTC(1/5)
                9  GGTCTC              
BsmAI         458  GAGAC                GTCTC(1/5)
               10  GTCTC               
EcoT14I       296  CCATGG               C^C[AT][AT]GG
              603  CCAAGG              
HphI          174  GGTGA                GGTGA(8/7)
              881  TCACC               
MaeIII         28  GTCAC                ^GT[ACGT]AC
              257  GTCAC               
MwoI          514  GCAAAAGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              769  GCTAGTTGGGC         
PshBI         117  ATTAAT               AT^TAAT
              121  ATTAAT              
SmlI           78  CTCAAG               C^T[CT][GA]AG
              844  CTTAAG              
StyI          296  CCATGG               C^C[AT][AT]GG
              603  CCAAGG              
Tsp45I         28  GTCAC                ^GT[GC]AC
              257  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        BbsI        BbvI        BfaI        BpuAI       
BsaJI       BssKI       BstNI       DdeI        DpnII       
EcoRII      Fnu4HI      ItaI        MaeI        MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      Sau3AI      ScrFI       TaqI        Tru9I       
TseI        Tsp509I     TthHB8I     
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