Restriction map for pF1KA0073
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AhdI        Alw44I      AocI        ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BanII       BbrPI       BbvCI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaI        BseAI       
BsiEI       BsiHKAI     BsiWI       BsmI        BsoBI       
BspDI       BspEI       BspMI       BsrBI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HgaI        HincII      HindII      HpaI        KpnI        
KspI        MaeI        MluI        MroI        MspA1I      
NaeI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII        1746  CGCG                 CG^CG
AcyI           44  GGCGCC               G[GA]^CG[CT]C
AflIII        333  ACATGT               A^C[GA][CT]GT
AgeI         1157  ACCGGT               A^CCGGT
Aor51HI       143  AGCGCT               AGC^GCT
BalI          179  TGGCCA               TGG^CCA
BamHI        1765  GGATCC               G^GATCC
BanI           44  GGCGCC               G^G[CT][GA]CC
BbeI           44  GGCGCC               GGCGC^C
BbsI          717  GTCTTC               GAAGAC(2/6)
BclI          500  TGATCA               T^GATCA
BmyI         1533  GTGCCC               G[AGT]GC[ACT]^C
BpmI         1631  CTGGAG               CTGGAG(16/14)
BpuAI         717  GTCTTC               GAAGAC(2/6)
BsaBI        1387  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          44  GGCGCC               G[GA]^CG[CT]C
BsgI         1382  GTGCAG               GTGCAG(16/14)
BsmBI          33  GAGACG               CGTCTC(1/5)
Bsp1286I     1533  GTGCCC               G[AGT]GC[ACT]^C
BspLU11I      333  ACATGT               A^CATGT
BsrDI         466  GCAATG               GCAATG(2/0)
BsrFI        1157  ACCGGT               [GA]^CCGG[CT]
BstUI        1746  CGCG                 CG^CG
BstXI        1792  CCAACGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          93  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I       1157  ACCGGT               [GA]^CCGG[CT]
DraIII       1102  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      143  AGCGCT               AGC^GCT
EcoT14I      1798  CCTTGG               C^C[AT][AT]GG
EcoT22I       262  ATGCAT               ATGCA^T
FauI         1747  GCGGG                CCCGC(4/6)
FbaI          500  TGATCA               T^GATCA
Hin1I          44  GGCGCC               G[GA]^CG[CT]C
HindIII      1265  AAGCTT               A^AGCTT
KasI           44  GGCGCC               G^GCGCC
MamI         1387  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1204  CAATTG               C^AATTG
MluNI         179  TGGCCA               TGG^CCA
MscI          179  TGGCCA               TGG^CCA
MunI         1204  CAATTG               C^AATTG
MvnI         1746  CGCG                 CG^CG
NarI           44  GGCGCC               GG^CGCC
NdeI         1668  CATATG               CA^TATG
NsiI          262  ATGCAT               ATGCA^T
PacI         1072  TTAATTAA             TTAAT^TAA
PinAI        1157  ACCGGT               A^CCGGT
SapI          139  GAAGAGC              GCTCTTC(1/4)
SfoI           44  GGCGCC               GGC^GCC
SmlI         1445  CTCAAG               C^T[CT][GA]AG
StyI         1798  CCTTGG               C^C[AT][AT]GG
TaqI         1395  TCGA                 T^CGA
TthHB8I      1395  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AseI         1071  ATTAAT               AT^TAAT
             1075  ATTAAT              
AsnI         1071  ATTAAT               AT^TAAT
             1075  ATTAAT              
BcnI           48  CCGGG                CC^[GC]GG
               54  CCCGG               
BsaWI          63  ACCGGA               [AT]^CCGG[AT]
             1157  ACCGGT              
BsmFI          50  GGGAC                GGGAC(10/14)
              114  GTCCC               
Bsp1407I     1318  TGTACA               T^GTACA
             1854  TGTACA              
BsrGI        1318  TGTACA               T^GTACA
             1854  TGTACA              
DraII          50  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1455  AGGACCT             
EcoO109I       50  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1455  AGGACCT             
HaeII          44  GGCGCC               [GA]GCGC^[CT]
              143  AGCGCT              
MwoI          172  GCAACACTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1441  GCTACTCAAGC         
NciI           48  CCGGG                CC^[GC]GG
               54  CCCGG               
PleI          212  GAGTC                GAGTC(4/5)
              947  GAGTC               
PpuMI          50  GGGACCC              [GA]G^G[AT]CC[CT]
             1455  AGGACCT             
PshBI        1071  ATTAAT               AT^TAAT
             1075  ATTAAT              
SfcI         1034  CTGTAG               C^T[GA][CT]AG
             1253  CTATAG              
SspBI        1318  TGTACA               T^GTACA
             1854  TGTACA              
Tsp45I        392  GTCAC                ^GT[GC]AC
             1834  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BbvI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BspHI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
CfoI        Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspI        MvaI        NdeII       NlaIII      
NlaIV       NspI        RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SspI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       XhoII       
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