(Circular) (Six-base) MAPSORT of: pFN21AE2780.seq Check: 879 from: 1 to: 7129 pFN21AE2780 7129 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4939 278 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4472 1937 Size: 2535 4594 Acc65I G'GTAC_C Cuts at: 2705 4450 2705 Size: 1745 5384 AclI AA'CG_TT Cuts at: 1563 5256 5629 1563 Size: 3693 373 3063 Fragments arranged by size: 3693 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2158 4055 4110 4188 4352 5192 6576 6844 Size: 526 1897 55 78 164 840 1384 268 Cuts at: 6844 1632 Size: 1917 Fragments arranged by size: 1917 1897 1384 840 526 268 164 78 55 AfeI AGC'GCT Cuts at: 2348 3527 2348 Size: 1179 5950 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6932 AflIII A'CryG_T Cuts at: 1203 6028 7005 1203 Size: 4825 977 1327 Fragments arranged by size: 4825 1327 977 AgeI A'CCGG_T Cuts at: 5960 5960 Size: 7129 AhdI GACnn_n'nnGTC Cuts at: 5858 5858 Size: 7129 AleI CACnn'nnGTG Cuts at: 1166 4358 1166 Size: 3192 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7129 AlwNI CAG_nnn'CTG Cuts at: 4277 4872 6444 4277 Size: 595 1572 4962 Fragments arranged by size: 4962 1572 595 ApaLI G'TGCA_C Cuts at: 5186 6342 5186 Size: 1156 5973 ApoI r'AATT_y Cuts at: 1493 3412 3654 4133 4438 4664 4816 6925 1493 Size: 1919 242 479 305 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 479 305 242 226 152 AseI AT'TA_AT Cuts at: 160 1032 2514 3284 3944 5683 160 Size: 872 1482 770 660 1739 1606 Fragments arranged by size: 1739 1606 1482 872 770 660 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 AvaI C'yCGr_G Cuts at: 1870 1943 4454 1870 Size: 73 2511 4545 Fragments arranged by size: 4545 2511 73 BamHI G'GATC_C Cuts at: 2393 4459 4826 2393 Size: 2066 367 4696 Fragments arranged by size: 4696 2066 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2705 3223 4450 5905 Size: 325 217 627 369 549 518 1227 1455 Cuts at: 5905 618 Size: 1842 Fragments arranged by size: 1842 1455 1227 627 549 518 369 325 217 BanII G_rGCy'C Cuts at: 1391 3576 4448 1391 Size: 2185 872 4072 Fragments arranged by size: 4072 2185 872 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 4061 4356 4408 928 Size: 971 1474 688 295 52 3649 Fragments arranged by size: 3649 1474 971 688 295 52 BbvCI CC'TCA_GC Cuts at: 3542 3542 Size: 7129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5320 5354 1903 Size: 34 3383 34 3678 Fragments arranged by size: 3678 3383 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5021 6231 5021 Size: 1210 5919 BclI T'GATC_A Cuts at: 1548 3088 3916 1548 Size: 1540 828 4761 Fragments arranged by size: 4761 1540 828 BfrBI ATG'CAT Cuts at: 3239 3420 3239 Size: 181 6948 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5740 136 Size: 107 122 71 631 605 4068 1525 Fragments arranged by size: 4068 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2085 7124 2085 Size: 5039 2090 BlpI GC'TnA_GC Cuts at: 4546 4546 Size: 7129 Bme1580I G_kGCm'C Cuts at: 1790 2140 2159 5190 6346 1790 Size: 350 19 3031 1156 2573 Fragments arranged by size: 3031 2573 1156 350 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4860 5818 442 Size: 416 53 83 399 24 3443 958 1753 Fragments arranged by size: 3443 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 3609 4326 5789 1239 Size: 1676 694 717 1463 2579 Fragments arranged by size: 2579 1676 1463 717 694 Bpu10I CC'TnA_GC Cuts at: 3542 3542 Size: 7129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5254 6119 6417 5254 Size: 865 298 5966 Fragments arranged by size: 5966 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5792 882 Size: 617 4293 2219 Fragments arranged by size: 4293 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4920 493 Size: 711 3716 2702 Fragments arranged by size: 3716 2702 711 BsaBI GATnn'nnATC Cuts at: 7123 7123 Size: 7129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 4936 5318 5953 Size: 53 83 186 936 825 2578 382 635 Cuts at: 5953 275 Size: 1451 Fragments arranged by size: 2578 1451 936 825 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5562 5960 6234 6381 6792 5562 Size: 398 274 147 411 5899 Fragments arranged by size: 5899 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436 Size: 30 165 30 1468 30 45 30 460 Cuts at: 3436 3466 4212 4242 1178 Size: 30 746 30 4065 Fragments arranged by size: 4065 1468 746 460 165 45 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2970 3875 1186 Size: 1204 580 905 4440 Fragments arranged by size: 4440 1204 905 580 BseYI C'CCAG_C Cuts at: 2281 6332 6828 2281 Size: 4051 496 2582 Fragments arranged by size: 4051 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6556 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4579 5340 6368 1540 Size: 275 186 2578 761 1028 2301 Fragments arranged by size: 2578 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2632 3576 5190 5275 6346 2632 Size: 944 1614 85 1071 3415 Fragments arranged by size: 3415 1614 1071 944 85 BsmI GAATG_Cn' Cuts at: 2497 4640 4733 2497 Size: 2143 93 4893 Fragments arranged by size: 4893 2143 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2793 2963 2793 Size: 170 6959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2159 2632 3576 4448 5190 5275 Size: 399 350 19 473 944 872 742 85 Cuts at: 5275 6346 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 944 872 742 473 399 350 85 19 BspEI T'CCGG_A Cuts at: 6792 6792 Size: 7129 BspHI T'CATG_A Cuts at: 3846 5018 6748 3846 Size: 1172 1730 4227 Fragments arranged by size: 4227 1730 1172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4484 844 Size: 3640 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5016 1394 Size: 429 3193 3507 Fragments arranged by size: 3507 3193 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5624 5798 65 Size: 1183 4376 174 1396 Fragments arranged by size: 4376 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5773 5960 1700 Size: 110 3963 187 2869 Fragments arranged by size: 3963 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7129 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7129 BssSI C'ACGA_G Cuts at: 2853 4251 5189 6201 2853 Size: 1398 938 1012 3781 Fragments arranged by size: 3781 1398 1012 938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7129 BstBI TT'CG_AA Cuts at: 5933 5933 Size: 7129 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7129 BstXI CCAn_nnnn'nTGG Cuts at: 2309 4036 2309 Size: 1727 5402 BstYI r'GATC_y Cuts at: 1972 2085 2393 3160 4394 4459 4826 5211 5228 Size: 113 308 767 1234 65 367 385 17 Cuts at: 5228 6680 6766 6778 7124 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1234 767 385 367 346 308 113 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2641 4177 513 Size: 552 1576 1536 3465 Fragments arranged by size: 3465 1576 1536 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6390 Fragments arranged by size: 6390 683 56 BtsI GCAGTG_nn' Cuts at: 4645 5439 5459 4645 Size: 794 20 6315 Fragments arranged by size: 6315 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7129 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7094 DraI TTT'AAA Cuts at: 3759 4433 3759 Size: 674 6455 DraIII CAC_nnn'GTG Cuts at: 2858 2858 Size: 7129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6136 809 Size: 507 4820 1802 Fragments arranged by size: 4820 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4576 5465 7094 Size: 54 1425 50 275 18 2746 889 1629 Cuts at: 7094 8 Size: 43 Fragments arranged by size: 2746 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4576 1537 Size: 275 2764 4090 Fragments arranged by size: 4090 2764 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2791 3684 5059 1353 Size: 1438 893 1375 3423 Fragments arranged by size: 3423 1438 1375 893 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5716 6090 6236 387 Size: 1064 138 4127 374 146 1280 Fragments arranged by size: 4127 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3574 3574 Size: 7129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2915 3609 4055 4110 4188 4326 Size: 393 526 757 694 446 55 78 138 Cuts at: 4326 4352 5192 5789 6576 6844 1239 Size: 26 840 597 787 268 1524 Fragments arranged by size: 1524 840 787 757 694 597 526 446 393 268 138 78 55 26 EcoO109I rG'GnC_Cy Cuts at: 2788 3094 4094 4274 2788 Size: 306 1000 180 5643 Fragments arranged by size: 5643 1000 306 180 EcoRI G'AATT_C Cuts at: 4133 4438 4816 4133 Size: 305 378 6446 Fragments arranged by size: 6446 378 305 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 7129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2413 2759 3338 4085 2188 Size: 225 346 579 747 5232 Fragments arranged by size: 5232 747 579 346 225 FspI TGC'GCA Cuts at: 5635 5635 Size: 7129 HaeII r_GCGC'y Cuts at: 1678 2350 3274 3529 6276 1678 Size: 672 924 255 2747 2531 Fragments arranged by size: 2747 2531 924 672 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946 Size: 32 135 32 505 32 623 32 182 Cuts at: 2946 2978 5770 5802 5844 5876 1373 Size: 32 2792 32 42 32 2626 Fragments arranged by size: 2792 2626 623 505 182 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 2529 4473 4719 669 Size: 988 281 115 476 1944 246 3079 Fragments arranged by size: 3079 1944 988 476 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 7129 HpaI GTT'AAC Cuts at: 2053 4719 2053 Size: 2666 4463 KpnI G_GTAC'C Cuts at: 2709 4454 2709 Size: 1745 5384 MfeI C'AATT_G Cuts at: 2988 4728 2988 Size: 1740 5389 MluI A'CGCG_T Cuts at: 7005 7005 Size: 7129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3889 6243 6427 1305 Size: 2584 2354 184 2007 Fragments arranged by size: 2584 2354 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5307 Fragments arranged by size: 5307 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2307 4358 5087 5446 5605 518 Size: 648 1141 2051 729 359 159 2042 Fragments arranged by size: 2051 2042 1141 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2762 3398 4545 5222 6370 6615 1606 Size: 1156 636 1147 677 1148 245 2120 Fragments arranged by size: 2120 1156 1148 1147 677 636 245 NcoI C'CATG_G Cuts at: 513 1065 2641 4177 513 Size: 552 1576 1536 3465 Fragments arranged by size: 3465 1576 1536 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7129 NotI GC'GGCC_GC Cuts at: 4576 4576 Size: 7129 NsiI A_TGCA'T Cuts at: 3241 3422 3241 Size: 181 6948 NspI r_CATG'y Cuts at: 1132 1229 4487 6032 1132 Size: 97 3258 1545 2229 Fragments arranged by size: 3258 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7129 PciI A'CATG_T Cuts at: 6028 6028 Size: 7129 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7129 PmeI GTTT'AAAC Cuts at: 4433 4433 Size: 7129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5198 5230 6711 6743 5198 Size: 32 1481 32 5584 Fragments arranged by size: 5584 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 4094 4274 3094 Size: 1000 180 5949 Fragments arranged by size: 5949 1000 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7129 PsiI TTA'TAA Cuts at: 4025 4699 4025 Size: 674 6455 PstI C_TGCA'G Cuts at: 830 3294 3562 4481 830 Size: 2464 268 919 3478 Fragments arranged by size: 3478 2464 919 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7129 PvuII CAG'CTG Cuts at: 2762 3398 2762 Size: 636 6493 SacI G_AGCT'C Cuts at: 3576 3576 Size: 7129 SalI G'TCGA_C Cuts at: 1936 4471 1936 Size: 2535 4594 SapI GCTCTTCn'nnn_ Cuts at: 3684 3684 Size: 7129 SbfI CC_TGCA'GG Cuts at: 4481 4481 Size: 7129 ScaI AGT'ACT Cuts at: 5377 7089 5377 Size: 1712 5417 SexAI A'CCwGG_T Cuts at: 3739 3739 Size: 7129 SfcI C'TryA_G Cuts at: 826 1045 3290 3558 4477 5612 6293 6484 826 Size: 219 2245 268 919 1135 681 191 1471 Fragments arranged by size: 2245 1471 1135 919 681 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7129 SmaI CCC'GGG Cuts at: 1872 4456 1872 Size: 2584 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5233 6134 6396 820 Size: 197 926 3290 901 262 1553 Fragments arranged by size: 3290 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4920 493 Size: 4427 2702 SpeI A'CTAG_T Cuts at: 152 3638 152 Size: 3486 3643 SphI G_CATG'C Cuts at: 1132 4487 1132 Size: 3355 3774 SspI AAT'ATT Cuts at: 5 52 5053 5 Size: 47 5001 2081 Fragments arranged by size: 5001 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7129 StyI C'CwwG_G Cuts at: 513 1065 2119 2532 2641 2791 4177 4568 513 Size: 552 1054 413 109 150 1386 391 3074 Fragments arranged by size: 3074 1386 1054 552 413 391 150 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5156 5173 5326 5511 5956 325 Size: 1255 3576 17 153 185 445 1498 Fragments arranged by size: 3576 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5375 7087 96 Size: 274 80 33 51 1443 3398 1712 138 Fragments arranged by size: 3398 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2871 2876 2903 3436 3468 4212 4244 7089 Size: 27 5 27 533 32 744 32 2845 Cuts at: 7089 7121 2844 Size: 32 2852 Fragments arranged by size: 2852 2845 744 533 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7129 XbaI T'CTAG_A Cuts at: 4397 4465 4397 Size: 68 7061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2603 2603 Size: 7129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7129 XmaI C'CCGG_G Cuts at: 1870 4454 1870 Size: 2584 4545 XmnI GAAnn'nnTTC Cuts at: 2171 5258 2171 Size: 3087 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4937 276 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI