(Linear) (Six-base) MAPSORT of: KIEE2780.seq Check: 5673 from: 1 to: 2424 KIEE2780.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. Acc65I G'GTAC_C Cuts at: 0 700 2424 Size: 700 1724 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 2050 2105 2183 2347 2424 Size: 153 1897 55 78 164 77 Fragments arranged by size: 1897 164 153 78 77 55 AfeI AGC'GCT Cuts at: 0 343 1522 2424 Size: 343 1179 902 Fragments arranged by size: 1179 902 343 AleI CACnn'nnGTG Cuts at: 0 2353 2424 Size: 2353 71 AlwNI CAG_nnn'CTG Cuts at: 0 2272 2424 Size: 2272 152 ApoI r'AATT_y Cuts at: 0 1407 1649 2128 2424 Size: 1407 242 479 296 Fragments arranged by size: 1407 479 296 242 AseI AT'TA_AT Cuts at: 0 509 1279 1939 2424 Size: 509 770 660 485 Fragments arranged by size: 770 660 509 485 BamHI G'GATC_C Cuts at: 0 388 2424 Size: 388 2036 BanI G'GyrC_C Cuts at: 0 151 700 1218 2424 Size: 151 549 518 1206 Fragments arranged by size: 1206 549 518 151 BanII G_rGCy'C Cuts at: 0 1571 2424 Size: 1571 853 BbsI GAAGACnn'nnnn_ Cuts at: 0 1368 2056 2351 2403 2424 Size: 1368 688 295 52 21 Fragments arranged by size: 1368 688 295 52 21 BbvCI CC'TCA_GC Cuts at: 0 1537 2424 Size: 1537 887 BclI T'GATC_A Cuts at: 0 1083 1911 2424 Size: 1083 828 513 Fragments arranged by size: 1083 828 513 BfrBI ATG'CAT Cuts at: 0 1234 1415 2424 Size: 1234 181 1009 Fragments arranged by size: 1234 1009 181 BglII A'GATC_T Cuts at: 0 80 2424 Size: 80 2344 Bme1580I G_kGCm'C Cuts at: 0 135 154 2424 Size: 135 19 2270 Fragments arranged by size: 2270 135 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1604 2321 2424 Size: 910 694 717 103 Fragments arranged by size: 910 717 694 103 Bpu10I CC'TnA_GC Cuts at: 0 1537 2424 Size: 1537 887 BsaHI Gr'CG_yC Cuts at: 0 353 2424 Size: 353 2071 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 866 896 941 971 1431 1461 2207 2237 Size: 866 30 45 30 460 30 746 30 Cuts at: 2237 2424 Size: 187 Fragments arranged by size: 866 746 460 187 45 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 965 1870 2424 Size: 385 580 905 554 Fragments arranged by size: 905 580 554 385 BseYI C'CCAG_C Cuts at: 0 276 2424 Size: 276 2148 BsiHKAI G_wGCw'C Cuts at: 0 627 1571 2424 Size: 627 944 853 Fragments arranged by size: 944 853 627 BsmI GAATG_Cn' Cuts at: 0 492 2424 Size: 492 1932 BsmBI CGTCTCn'nnnn_ Cuts at: 0 788 958 2424 Size: 788 170 1466 Fragments arranged by size: 1466 788 170 Bsp1286I G_dGCh'C Cuts at: 0 135 154 627 1571 2424 Size: 135 19 473 944 853 Fragments arranged by size: 944 853 473 135 19 BspHI T'CATG_A Cuts at: 0 1841 2424 Size: 1841 583 BssSI C'ACGA_G Cuts at: 0 848 2246 2424 Size: 848 1398 178 Fragments arranged by size: 1398 848 178 BstXI CCAn_nnnn'nTGG Cuts at: 0 304 2031 2424 Size: 304 1727 393 Fragments arranged by size: 1727 393 304 BstYI r'GATC_y Cuts at: 0 80 388 1155 2389 2424 Size: 80 308 767 1234 35 Fragments arranged by size: 1234 767 308 80 35 BtgI C'CryG_G Cuts at: 0 636 2172 2424 Size: 636 1536 252 Fragments arranged by size: 1536 636 252 DraI TTT'AAA Cuts at: 0 1754 2424 Size: 1754 670 DraIII CAC_nnn'GTG Cuts at: 0 853 2424 Size: 853 1571 EarI CTCTTCn'nnn_ Cuts at: 0 786 1679 2424 Size: 786 893 745 Fragments arranged by size: 893 786 745 EcoICRI GAG'CTC Cuts at: 0 1569 2424 Size: 1569 855 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 910 1604 2050 2105 2183 2321 2347 Size: 153 757 694 446 55 78 138 26 Cuts at: 2347 2424 Size: 77 Fragments arranged by size: 757 694 446 153 138 78 77 55 26 EcoO109I rG'GnC_Cy Cuts at: 0 783 1089 2089 2269 2424 Size: 783 306 1000 180 155 Fragments arranged by size: 1000 783 306 180 155 EcoRI G'AATT_C Cuts at: 0 2128 2424 Size: 2128 296 EcoRV GAT'ATC Cuts at: 0 444 2424 Size: 444 1980 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 183 408 754 1333 2080 2424 Size: 183 225 346 579 747 344 Fragments arranged by size: 747 579 346 344 225 183 HaeII r_GCGC'y Cuts at: 0 345 1269 1524 2424 Size: 345 924 255 900 Fragments arranged by size: 924 900 345 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 72 104 727 759 941 973 2424 Size: 72 32 623 32 182 32 1451 Fragments arranged by size: 1451 623 182 72 32 32 32 HincII GTy'rAC Cuts at: 0 48 524 2424 Size: 48 476 1900 Fragments arranged by size: 1900 476 48 HpaI GTT'AAC Cuts at: 0 48 2424 Size: 48 2376 KpnI G_GTAC'C Cuts at: 0 704 2424 Size: 704 1720 MfeI C'AATT_G Cuts at: 0 983 2424 Size: 983 1441 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1884 2424 Size: 1884 540 MslI CAynn'nnrTG Cuts at: 0 302 2353 2424 Size: 302 2051 71 Fragments arranged by size: 2051 302 71 MspA1I CmG'CkG Cuts at: 0 757 1393 2424 Size: 757 636 1031 Fragments arranged by size: 1031 757 636 NcoI C'CATG_G Cuts at: 0 636 2172 2424 Size: 636 1536 252 Fragments arranged by size: 1536 636 252 NsiI A_TGCA'T Cuts at: 0 1236 1417 2424 Size: 1236 181 1007 Fragments arranged by size: 1236 1007 181 PpuMI rG'GwC_Cy Cuts at: 0 1089 2089 2269 2424 Size: 1089 1000 180 155 Fragments arranged by size: 1089 1000 180 155 PsiI TTA'TAA Cuts at: 0 2020 2424 Size: 2020 404 PstI C_TGCA'G Cuts at: 0 1289 1557 2424 Size: 1289 268 867 Fragments arranged by size: 1289 867 268 PvuII CAG'CTG Cuts at: 0 757 1393 2424 Size: 757 636 1031 Fragments arranged by size: 1031 757 636 SacI G_AGCT'C Cuts at: 0 1571 2424 Size: 1571 853 SapI GCTCTTCn'nnn_ Cuts at: 0 1679 2424 Size: 1679 745 SexAI A'CCwGG_T Cuts at: 0 1734 2424 Size: 1734 690 SfcI C'TryA_G Cuts at: 0 1285 1553 2424 Size: 1285 268 871 Fragments arranged by size: 1285 871 268 SpeI A'CTAG_T Cuts at: 0 1633 2424 Size: 1633 791 StyI C'CwwG_G Cuts at: 0 114 527 636 786 2172 2424 Size: 114 413 109 150 1386 252 Fragments arranged by size: 1386 413 252 150 114 109 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 866 871 898 1431 1463 2207 2239 Size: 839 27 5 27 533 32 744 32 Cuts at: 2239 2424 Size: 185 Fragments arranged by size: 839 744 533 185 32 32 27 27 5 XbaI T'CTAG_A Cuts at: 0 2392 2424 Size: 2392 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 598 2424 Size: 598 1826 XmnI GAAnn'nnTTC Cuts at: 0 166 2424 Size: 166 2258 Enzymes that do cut: Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI BstXI BstYI BtgI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI MspA1I NcoI NsiI PpuMI PsiI PstI PvuII SacI SapI SexAI SfcI SpeI StyI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI FspI FspAI HindIII KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI ZraI