Restriction map for
pFN21AE9246
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AviII AvrII BalI BamHI BbeI
BlnI BsaBI BsiWI Bsp1407I BsrBI
BsrFI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DraIII
Eco47III EcoNI EcoO65I EcoRV FseI
FspI HpaI KasI KpnI KspI
MamI MluI MluNI MscI NaeI
NarI NgoMIV NheI NruI NspV
PacI PinAI PmeI PpuMI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 3676 GACGTC GACGT^C
AccI 2696 GTAGAC GT^[AC][GT]AC
AccIII 93 TCCGGA T^CCGGA
AcyI 3676 GACGTC G[GA]^CG[CT]C
AflII 4962 CTTAAG C^TTAAG
AhdI 4298 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2053 ATTAAT AT^TAAT
AsnI 2053 ATTAAT AT^TAAT
AspEI 4298 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2371 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2959 GGCACC G^G[CT][GA]CC
BcgI 3315 CGATTCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4962 CTTAAG C^TTAAG
BglI 273 GCCCGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1021 AGATCT A^GATCT
BlpI 145 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 145 GCTGAGC GC^T[ACGT]AGC
BsaHI 3676 GACGTC G[GA]^CG[CT]C
BsaWI 93 TCCGGA [AT]^CCGG[AT]
BseAI 93 TCCGGA T^CCGGA
BsgI 3220 GTGCAG GTGCAG(16/14)
BsiEI 245 CGGCCG CG[GA][CT]^CG
BspDI 560 ATCGAT AT^CGAT
BspEI 93 TCCGGA T^CCGGA
BspLU11I 597 ACATGT A^CATGT
BspMI 3853 GCAGGT ACCTGC(4/8)
BssHII 2344 GCGCGC G^CGCGC
BstAPI 3000 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 145 GCTGAGC GC^T[ACGT]AGC
ClaI 560 ATCGAT AT^CGAT
DraII 261 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 245 CGGCCG [CT]^GGCC[GA]
EagI 245 CGGCCG C^GGCCG
Eam1105I 4298 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 245 CGGCCG C^GGCCG
Eco52I 245 CGGCCG C^GGCCG
EcoO109I 261 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1621 GAATTC G^AATTC
HgaI 4167 GACGC GACGC(5/10)
Hin1I 3676 GACGTC G[GA]^CG[CT]C
MfeI 4221 CAATTG C^AATTG
MroI 93 TCCGGA T^CCGGA
MunI 4221 CAATTG C^AATTG
NotI 244 GCGGCCGC GC^GGCCGC
PflFI 2371 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4444 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2699 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2053 ATTAAT AT^TAAT
PstI 3032 CTGCAG CTGCA^G
PvuII 2791 CAGCTG CAG^CTG
ScaI 4069 AGTACT AGT^ACT
SspI 382 AATATT AAT^ATT
Tth111I 2371 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4444 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AocI 220 CCTCAGG CC^T[ACGT]AGG
2387 CCTTAGG
AspHI 2397 GAGCAC G[AT]GC[AT]^C
3727 GTGCTC
AvaII 807 GGACC G^G[AT]CC
1252 GGTCC
BanII 148 GAGCCC G[GA]GC[CT]^C
271 GAGCCC
BbrPI 832 CACGTG CAC^GTG
4607 CACGTG
BbvCI 204 CCTCAGC CCTCAGC(-5/-2)
2421 GCTGAGG
BciVI 1396 GGATAC GTATCC(6/5)
2274 GGATAC
BclI 1197 TGATCA T^GATCA
3522 TGATCA
BsaAI 832 CACGTG [CT]AC^GT[GA]
4607 CACGTG
BsaI 1388 GAGACC GGTCTC(1/5)
4594 GAGACC
BsiHKAI 2397 GAGCAC G[AT]GC[AT]^C
3727 GTGCTC
BspHI 3668 TCATGA T^CATGA
3708 TCATGA
BssSI 211 CACGAG CACGAG(-5/-1)
1136 CTCGTG
BstXI 111 CCAACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2579 CCAGCGAAGTGG
Bsu36I 220 CCTCAGG CC^T[ACGT]AGG
2387 CCTTAGG
DrdI 944 GACACGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1449 GACTGCCAAGTC
DsaI 638 CCATGG C^C[GA][CT]GG
761 CCATGG
Eco81I 220 CCTCAGG CC^T[ACGT]AGG
2387 CCTTAGG
EcoT22I 542 ATGCAT ATGCA^T
2357 ATGCAT
FauI 181 GCGGG CCCGC(4/6)
238 GCGGG
FbaI 1197 TGATCA T^GATCA
3522 TGATCA
HindIII 2137 AAGCTT A^AGCTT
4932 AAGCTT
MspA1I 153 CAGCGG C[AC]G^C[GT]G
2791 CAGCTG
NcoI 638 CCATGG C^CATGG
761 CCATGG
NsiI 542 ATGCAT ATGCA^T
2357 ATGCAT
PaeR7I 359 CTCGAG C^TCGAG
1460 CTCGAG
PmaCI 832 CACGTG CAC^GTG
4607 CACGTG
PmlI 832 CACGTG CAC^GTG
4607 CACGTG
RcaI 3668 TCATGA T^CATGA
3708 TCATGA
SfcI 432 CTACAG C^T[GA][CT]AG
3032 CTGCAG
SmaI 2888 CCCGGG CCC^GGG
3548 CCCGGG
SphI 1559 GCATGC GCATG^C
1806 GCATGC
XcmI 3500 CCACAGAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4075 CCAGAAGGAAAATGG
XhoI 359 CTCGAG C^TCGAG
1460 CTCGAG
XmaCI 2888 CCCGGG C^CCGGG
3548 CCCGGG
XmaI 2888 CCCGGG C^CCGGG
3548 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AvaI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI