(Circular) (Six-base) MAPSORT of: pFN21AE9246.seq Check: 2066 from: 1 to: 9715 pFN21AE9246 9715 bp With 182 enzymes: SgfI * November 16, 2016 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5685 7525 278 Size: 53 83 186 936 4149 1840 2468 Fragments arranged by size: 4149 2468 1840 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4702 7058 1937 Size: 2765 2356 4594 Fragments arranged by size: 4594 2765 2356 Acc65I G'GTAC_C Cuts at: 7036 7036 Size: 9715 AclI AA'CG_TT Cuts at: 1563 7842 8215 1563 Size: 6279 373 3063 Fragments arranged by size: 6279 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 3852 3875 5496 5667 6263 6402 6729 Size: 893 1327 23 1621 171 596 139 327 Cuts at: 6729 6762 7778 9162 9430 1632 Size: 33 1016 1384 268 1917 Fragments arranged by size: 1917 1621 1384 1327 1016 893 596 327 268 171 139 33 23 AflII C'TTAA_G Cuts at: 820 1017 6967 820 Size: 197 5950 3568 Fragments arranged by size: 5950 3568 197 AflIII A'CryG_T Cuts at: 1203 2602 2838 2950 4980 8614 9591 1203 Size: 1399 236 112 2030 3634 977 1327 Fragments arranged by size: 3634 2030 1399 1327 977 236 112 AgeI A'CCGG_T Cuts at: 8546 8546 Size: 9715 AhdI GACnn_n'nnGTC Cuts at: 6308 8444 6308 Size: 2136 7579 AleI CACnn'nnGTG Cuts at: 1166 6228 1166 Size: 5062 4653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9715 AlwNI CAG_nnn'CTG Cuts at: 7458 9030 7458 Size: 1572 8143 ApaLI G'TGCA_C Cuts at: 7772 8928 7772 Size: 1156 8559 ApoI r'AATT_y Cuts at: 1493 2369 2428 3626 3649 3683 3761 3956 4630 Size: 876 59 1198 23 34 78 195 674 Cuts at: 4630 5296 5623 5702 6046 6113 6210 7024 7250 Size: 666 327 79 344 67 97 814 226 Cuts at: 7250 7402 9511 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1198 876 814 674 666 344 327 226 195 152 97 79 78 67 59 34 23 AseI AT'TA_AT Cuts at: 160 1032 4059 8269 160 Size: 872 3027 4210 1606 Fragments arranged by size: 4210 3027 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9715 AvaI C'yCGr_G Cuts at: 1870 1943 2364 3465 4893 5553 7040 1870 Size: 73 421 1101 1428 660 1487 4545 Fragments arranged by size: 4545 1487 1428 1101 660 421 73 BamHI G'GATC_C Cuts at: 7045 7412 7045 Size: 367 9348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4964 7036 8491 618 Size: 325 217 627 3177 2072 1455 1842 Fragments arranged by size: 3177 2072 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2157 2280 7034 1391 Size: 766 123 4754 4072 Fragments arranged by size: 4754 4072 766 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2947 4797 5856 6390 928 Size: 971 1048 1850 1059 534 4253 Fragments arranged by size: 4253 1850 1059 1048 971 534 BbvCI CC'TCA_GC Cuts at: 2210 4427 2210 Size: 2217 7498 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5309 5343 7906 7940 1903 Size: 34 3372 34 2563 34 3678 Fragments arranged by size: 3678 3372 2563 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3395 4273 7607 8817 3395 Size: 878 3334 1210 4293 Fragments arranged by size: 4293 3334 1210 878 BclI T'GATC_A Cuts at: 1548 3202 5527 1548 Size: 1654 2325 5736 Fragments arranged by size: 5736 2325 1654 BfrBI ATG'CAT Cuts at: 2549 4364 2549 Size: 1815 7900 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2284 8326 136 Size: 107 122 71 631 605 612 6042 1525 Fragments arranged by size: 6042 1525 631 612 605 122 107 71 BglII A'GATC_T Cuts at: 3026 9710 3026 Size: 6684 3031 BlpI GC'TnA_GC Cuts at: 2151 7132 2151 Size: 4981 4734 Bme1580I G_kGCm'C Cuts at: 1790 4598 4967 7776 8932 1790 Size: 2808 369 2809 1156 2573 Fragments arranged by size: 2809 2808 2573 1156 369 BmgBI CAC'GTC Cuts at: 1318 2444 4981 1318 Size: 1126 2537 6052 Fragments arranged by size: 6052 2537 1126 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4668 5580 7446 Size: 416 53 83 399 24 3251 912 1866 Cuts at: 7446 8404 442 Size: 958 1753 Fragments arranged by size: 3251 1866 1753 958 912 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9715 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4471 4471 Size: 9715 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2340 3210 6000 6063 6237 8375 1239 Size: 1101 870 2790 63 174 2138 2579 Fragments arranged by size: 2790 2579 2138 1101 870 174 63 Bpu10I CC'TnA_GC Cuts at: 2210 2933 4427 2210 Size: 723 1494 7498 Fragments arranged by size: 7498 1494 723 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2126 3961 4166 4282 5362 5426 6083 6494 6882 Size: 1835 205 116 1080 64 657 411 388 Cuts at: 6882 7840 8705 9003 2126 Size: 958 865 298 2838 Fragments arranged by size: 2838 1835 1080 958 865 657 411 388 298 205 116 64 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3387 6593 8378 882 Size: 617 1888 3206 1785 2219 Fragments arranged by size: 3206 2219 1888 1785 617 BsaAI yAC'GTr Cuts at: 493 1204 2839 6614 7506 493 Size: 711 1635 3775 892 2702 Fragments arranged by size: 3775 2702 1635 892 711 BsaBI GATnn'nnATC Cuts at: 9709 9709 Size: 9715 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5682 7522 7904 8539 Size: 53 83 186 936 4149 1840 382 635 Cuts at: 8539 275 Size: 1451 Fragments arranged by size: 4149 1840 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2098 8148 8546 8820 8967 9378 2098 Size: 6050 398 274 147 411 2435 Fragments arranged by size: 6050 2435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 4991 5021 1178 Size: 30 165 30 690 30 2868 30 5872 Fragments arranged by size: 5872 2868 690 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2224 2311 5015 1186 Size: 1006 32 87 2704 5886 Fragments arranged by size: 5886 2704 1006 87 32 BseYI C'CCAG_C Cuts at: 2156 2168 4798 8918 9414 2156 Size: 12 2630 4120 496 2457 Fragments arranged by size: 4120 2630 2457 496 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5246 1163 Size: 573 3510 5632 Fragments arranged by size: 5632 3510 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 7165 7926 8954 1540 Size: 275 186 252 4912 761 1028 2301 Fragments arranged by size: 4912 2301 1028 761 275 252 186 BsiHKAI G_wGCw'C Cuts at: 4406 5736 7776 7861 8932 4406 Size: 1330 2040 85 1071 5189 Fragments arranged by size: 5189 2040 1330 1071 85 BsmI GAATG_Cn' Cuts at: 2854 4780 5455 6154 7226 7319 2854 Size: 1926 675 699 1072 93 5250 Fragments arranged by size: 5250 1926 1072 699 675 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2015 2067 6663 2015 Size: 52 4596 5067 Fragments arranged by size: 5067 4596 52 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2280 4406 4598 4967 5736 7034 Size: 399 367 123 2126 192 369 769 1298 Cuts at: 7034 7776 7861 8932 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 2126 1298 1071 769 742 399 369 367 192 123 85 BspEI T'CCGG_A Cuts at: 2098 9378 2098 Size: 7280 2435 BspHI T'CATG_A Cuts at: 5673 5713 7604 9334 5673 Size: 40 1891 1730 6054 Fragments arranged by size: 6054 1891 1730 40 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5849 7070 844 Size: 5005 1221 3489 Fragments arranged by size: 5005 3489 1221 BsrBI CCG'CTC Cuts at: 1394 1823 7602 1394 Size: 429 5779 3507 Fragments arranged by size: 5779 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2712 4296 5853 6101 8210 8384 65 Size: 1183 1464 1584 1557 248 2109 174 1396 Fragments arranged by size: 2109 1584 1557 1464 1396 1183 248 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8359 8546 1700 Size: 110 6549 187 2869 Fragments arranged by size: 6549 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9715 BssHII G'CGCG_C Cuts at: 1925 4349 1925 Size: 2424 7291 BssSI C'ACGA_G Cuts at: 2216 3141 7775 8787 2216 Size: 925 4634 1012 3144 Fragments arranged by size: 4634 3144 1012 925 BstAPI GCAn_nnn'nTGC Cuts at: 794 5011 794 Size: 4217 5498 BstBI TT'CG_AA Cuts at: 8519 8519 Size: 9715 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9715 BstXI CCAn_nnnn'nTGG Cuts at: 2123 4591 2123 Size: 2468 7247 BstYI r'GATC_y Cuts at: 1972 2307 3026 4150 4624 7045 7412 7797 7814 Size: 335 719 1124 474 2421 367 385 17 Cuts at: 7814 9266 9352 9364 9710 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2421 1977 1452 1124 719 474 385 367 346 335 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2226 4393 2226 Size: 2167 7548 BtgI C'CryG_G Cuts at: 513 1065 2004 2643 2766 513 Size: 552 939 639 123 7462 Fragments arranged by size: 7462 939 639 552 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2131 487 Size: 683 56 905 8071 Fragments arranged by size: 8071 905 683 56 BtsI GCAGTG_nn' Cuts at: 5012 5046 7231 8025 8045 5012 Size: 34 2185 794 20 6682 Fragments arranged by size: 6682 2185 794 34 20 ClaI AT'CG_AT Cuts at: 1554 2566 1554 Size: 1012 8703 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9680 DraI TTT'AAA Cuts at: 2728 3096 5895 6403 7019 2728 Size: 368 2799 508 616 5424 Fragments arranged by size: 5424 2799 616 508 368 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2955 3460 8722 809 Size: 507 1639 505 5262 1802 Fragments arranged by size: 5262 1802 1639 507 505 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 7162 8051 Size: 54 1425 50 275 18 420 4912 889 Cuts at: 8051 9680 8 Size: 1629 43 Fragments arranged by size: 4912 1629 1425 889 420 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2250 7162 1537 Size: 275 438 4912 4090 Fragments arranged by size: 4912 4090 438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2268 2312 2491 4130 4212 4416 4725 5097 Size: 915 44 179 1639 82 204 309 372 Cuts at: 5097 5718 6510 6705 7645 1353 Size: 621 792 195 940 3423 Fragments arranged by size: 3423 1639 940 915 792 621 372 309 204 195 179 82 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2085 8302 8676 8822 387 Size: 1064 138 496 6217 374 146 1280 Fragments arranged by size: 6217 1280 1064 496 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2525 3210 3852 3875 5496 5667 Size: 393 708 185 685 642 23 1621 171 Cuts at: 5667 6000 6063 6237 6263 6402 6729 6762 7778 Size: 333 63 174 26 139 327 33 1016 Cuts at: 7778 8375 9162 9430 1239 Size: 597 787 268 1524 Fragments arranged by size: 1621 1524 1016 787 708 685 642 597 393 333 327 268 185 174 171 139 63 33 26 23 EcoO109I rG'GnC_Cy Cuts at: 2267 2267 Size: 9715 EcoRI G'AATT_C Cuts at: 3626 7024 7402 3626 Size: 3398 378 5939 Fragments arranged by size: 5939 3398 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3107 3125 6461 7005 3107 Size: 18 3336 544 5817 Fragments arranged by size: 5817 3336 544 18 FspI TGC'GCA Cuts at: 8221 8221 Size: 9715 HaeII r_GCGC'y Cuts at: 1678 2096 2169 3645 8862 1678 Size: 418 73 1476 5217 2531 Fragments arranged by size: 5217 2531 1476 418 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2548 2580 3187 Size: 32 135 32 521 32 423 32 607 Cuts at: 3187 3219 3421 3453 3601 3633 4541 4573 5426 Size: 32 202 32 148 32 908 32 853 Cuts at: 5426 5458 5512 5544 5973 6005 6294 6326 6467 Size: 32 54 32 429 32 289 32 141 Cuts at: 6467 6499 6509 6533 6541 6565 6638 6670 8356 Size: 32 10 24 8 24 73 32 1686 Cuts at: 8356 8388 8430 8462 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1686 908 853 607 521 429 423 289 202 148 141 135 73 54 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 24 24 10 8 HincII GTy'rAC Cuts at: 669 1657 1938 4384 4986 6171 7059 7305 669 Size: 988 281 2446 602 1185 888 246 3079 Fragments arranged by size: 3079 2446 1185 988 888 602 281 246 HindIII A'AGCT_T Cuts at: 748 4142 6937 748 Size: 3394 2795 3526 Fragments arranged by size: 3526 3394 2795 HpaI GTT'AAC Cuts at: 7305 7305 Size: 9715 KpnI G_GTAC'C Cuts at: 7040 7040 Size: 9715 MfeI C'AATT_G Cuts at: 6226 7314 6226 Size: 1088 8627 MluI A'CGCG_T Cuts at: 9591 9591 Size: 9715 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8829 9013 1305 Size: 7524 184 2007 Fragments arranged by size: 7524 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7893 Fragments arranged by size: 7893 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4212 4359 4443 6228 6335 6837 7673 Size: 648 3046 147 84 1785 107 502 836 Cuts at: 7673 8032 8191 518 Size: 359 159 2042 Fragments arranged by size: 3046 2042 1785 836 648 502 359 159 147 107 84 MspA1I CmG'CkG Cuts at: 1606 2160 4798 7131 7808 8956 9201 1606 Size: 554 2638 2333 677 1148 245 2120 Fragments arranged by size: 2638 2333 2120 1148 677 554 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2643 2766 513 Size: 552 939 639 123 7462 Fragments arranged by size: 7462 939 639 552 123 NdeI CA'TA_TG Cuts at: 387 2585 2962 3442 3736 5131 6874 387 Size: 2198 377 480 294 1395 1743 3228 Fragments arranged by size: 3228 2198 1743 1395 480 377 294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9715 NotI GC'GGCC_GC Cuts at: 2250 7162 2250 Size: 4912 4803 NsiI A_TGCA'T Cuts at: 2551 4366 2551 Size: 1815 7900 NspI r_CATG'y Cuts at: 1132 1229 2606 3479 3568 3815 5363 5504 7073 Size: 97 1377 873 89 247 1548 141 1569 Cuts at: 7073 8618 1132 Size: 1545 2229 Fragments arranged by size: 2229 1569 1548 1545 1377 873 247 141 97 89 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9715 PciI A'CATG_T Cuts at: 2602 8614 2602 Size: 6012 3703 PflMI CCAn_nnn'nTGG Cuts at: 1108 6455 1108 Size: 5347 4368 PmeI GTTT'AAAC Cuts at: 7019 7019 Size: 9715 PmlI CAC'GTG Cuts at: 2839 6614 2839 Size: 3775 5940 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7784 7816 9297 9329 7784 Size: 32 1481 32 8170 Fragments arranged by size: 8170 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4708 1613 Size: 3095 6620 PsiI TTA'TAA Cuts at: 3240 5630 7285 3240 Size: 2390 1655 5670 Fragments arranged by size: 5670 2390 1655 PstI C_TGCA'G Cuts at: 830 5041 7067 830 Size: 4211 2026 3478 Fragments arranged by size: 4211 3478 2026 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9715 PvuII CAG'CTG Cuts at: 4798 4798 Size: 9715 SalI G'TCGA_C Cuts at: 1936 7057 1936 Size: 5121 4594 SapI GCTCTTCn'nnn_ Cuts at: 2268 2312 4416 2268 Size: 44 2104 7567 Fragments arranged by size: 7567 2104 44 SbfI CC_TGCA'GG Cuts at: 7067 7067 Size: 9715 ScaI AGT'ACT Cuts at: 6076 7963 9675 6076 Size: 1887 1712 6116 Fragments arranged by size: 6116 1887 1712 SexAI A'CCwGG_T Cuts at: 2571 4825 5539 2571 Size: 2254 714 6747 Fragments arranged by size: 6747 2254 714 SfcI C'TryA_G Cuts at: 826 1045 2437 5037 7063 8198 8879 9070 826 Size: 219 1392 2600 2026 1135 681 191 1471 Fragments arranged by size: 2600 2026 1471 1392 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9715 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9715 SmaI CCC'GGG Cuts at: 1872 4895 5555 7042 1872 Size: 3023 660 1487 4545 Fragments arranged by size: 4545 3023 1487 660 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 2364 3465 3976 4145 4261 Size: 197 926 162 259 1101 511 169 116 Cuts at: 4261 5377 5405 6062 6473 6897 6967 7819 8720 Size: 1116 28 657 411 424 70 852 901 Cuts at: 8720 8982 820 Size: 262 1553 Fragments arranged by size: 1553 1116 1101 926 901 852 657 511 424 411 262 259 197 169 162 116 70 28 SnaBI TAC'GTA Cuts at: 493 7506 493 Size: 7013 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9715 SphI G_CATG'C Cuts at: 1132 3568 3815 7073 1132 Size: 2436 247 3258 3774 Fragments arranged by size: 3774 3258 2436 247 SspI AAT'ATT Cuts at: 5 52 2389 7639 5 Size: 47 2337 5250 2081 Fragments arranged by size: 5250 2337 2081 47 StuI AGG'CCT Cuts at: 1517 2030 2287 4877 1517 Size: 513 257 2590 6355 Fragments arranged by size: 6355 2590 513 257 StyI C'CwwG_G Cuts at: 513 1065 2004 2643 2766 5286 7154 513 Size: 552 939 639 123 2520 1868 3074 Fragments arranged by size: 3074 2520 1868 939 639 552 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2636 2699 2759 3411 4079 7742 7759 Size: 1255 1056 63 60 652 668 3663 17 Cuts at: 7759 7912 8097 8542 325 Size: 153 185 445 1498 Fragments arranged by size: 3663 1498 1255 1056 668 652 445 185 153 63 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5076 5138 6074 Size: 274 80 33 51 1443 3099 62 936 Cuts at: 6074 7961 9673 96 Size: 1887 1712 138 Fragments arranged by size: 3099 1887 1712 1443 936 274 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2589 2621 4069 4101 4795 4827 4991 5023 9675 Size: 32 1448 32 694 32 164 32 4652 Cuts at: 9675 9707 2589 Size: 32 2597 Fragments arranged by size: 4652 2597 1448 694 164 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4379 1316 Size: 3063 6652 XbaI T'CTAG_A Cuts at: 7051 7051 Size: 9715 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5512 6087 5512 Size: 575 9140 XhoI C'TCGA_G Cuts at: 1943 2364 3465 1943 Size: 421 1101 8193 Fragments arranged by size: 8193 1101 421 XmaI C'CCGG_G Cuts at: 1870 4893 5553 7040 1870 Size: 3023 660 1487 4545 Fragments arranged by size: 4545 3023 1487 660 XmnI GAAnn'nnTTC Cuts at: 6006 6444 6914 7844 6006 Size: 438 470 930 7877 Fragments arranged by size: 7877 930 470 438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5683 7523 276 Size: 53 83 186 936 4149 1840 2468 Fragments arranged by size: 4149 2468 1840 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI