(Linear) (Six-base) MAPSORT of: KIEE9246.seq Check: 5884 from: 1 to: 5010 KIEE9246.orf With 182 enzymes: SgfI * November 16, 2016 14:56 .. AatII G_ACGT'C Cuts at: 0 3680 5010 Size: 3680 1330 AccI GT'mk_AC Cuts at: 0 2697 5010 Size: 2697 2313 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 1847 1870 3491 3662 4258 4397 4724 Size: 520 1327 23 1621 171 596 139 327 Cuts at: 4724 4757 5010 Size: 33 253 Fragments arranged by size: 1621 1327 596 520 327 253 171 139 33 23 AflII C'TTAA_G Cuts at: 0 4962 5010 Size: 4962 48 AflIII A'CryG_T Cuts at: 0 597 833 945 2975 5010 Size: 597 236 112 2030 2035 Fragments arranged by size: 2035 2030 597 236 112 AhdI GACnn_n'nnGTC Cuts at: 0 4303 5010 Size: 4303 707 AleI CACnn'nnGTG Cuts at: 0 4223 5010 Size: 4223 787 ApoI r'AATT_y Cuts at: 0 364 423 1621 1644 1678 1756 1951 2625 Size: 364 59 1198 23 34 78 195 674 Cuts at: 2625 3291 3618 3697 4041 4108 4205 5010 Size: 666 327 79 344 67 97 805 Fragments arranged by size: 1198 805 674 666 364 344 327 195 97 79 78 67 59 34 23 AseI AT'TA_AT Cuts at: 0 2054 5010 Size: 2054 2956 AvaI C'yCGr_G Cuts at: 0 359 1460 2888 3548 5010 Size: 359 1101 1428 660 1462 Fragments arranged by size: 1462 1428 1101 660 359 BanI G'GyrC_C Cuts at: 0 2959 5010 Size: 2959 2051 BanII G_rGCy'C Cuts at: 0 152 275 5010 Size: 152 123 4735 Fragments arranged by size: 4735 152 123 BbsI GAAGACnn'nnnn_ Cuts at: 0 942 2792 3851 4385 5010 Size: 942 1850 1059 534 625 Fragments arranged by size: 1850 1059 942 625 534 BbvCI CC'TCA_GC Cuts at: 0 205 2422 5010 Size: 205 2217 2588 Fragments arranged by size: 2588 2217 205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3304 3338 5010 Size: 3304 34 1672 Fragments arranged by size: 3304 1672 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1390 2268 5010 Size: 1390 878 2742 Fragments arranged by size: 2742 1390 878 BclI T'GATC_A Cuts at: 0 1197 3522 5010 Size: 1197 2325 1488 Fragments arranged by size: 2325 1488 1197 BfrBI ATG'CAT Cuts at: 0 544 2359 5010 Size: 544 1815 2651 Fragments arranged by size: 2651 1815 544 BglI GCCn_nnn'nGGC Cuts at: 0 279 5010 Size: 279 4731 BglII A'GATC_T Cuts at: 0 1021 5010 Size: 1021 3989 BlpI GC'TnA_GC Cuts at: 0 146 5010 Size: 146 4864 Bme1580I G_kGCm'C Cuts at: 0 2593 2962 5010 Size: 2593 369 2048 Fragments arranged by size: 2593 2048 369 BmgBI CAC'GTC Cuts at: 0 439 2976 5010 Size: 439 2537 2034 Fragments arranged by size: 2537 2034 439 BmrI ACTGGGnnnn_n' Cuts at: 0 2663 3575 5010 Size: 2663 912 1435 Fragments arranged by size: 2663 1435 912 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2466 5010 Size: 2466 2544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 335 1205 3995 4058 4232 5010 Size: 335 870 2790 63 174 778 Fragments arranged by size: 2790 870 778 335 174 63 Bpu10I CC'TnA_GC Cuts at: 0 205 928 2422 5010 Size: 205 723 1494 2588 Fragments arranged by size: 2588 1494 723 205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1956 2161 2277 3357 3421 4078 4489 Size: 121 1835 205 116 1080 64 657 411 Cuts at: 4489 4877 5010 Size: 388 133 Fragments arranged by size: 1835 1080 657 411 388 205 133 121 116 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 1382 4588 5010 Size: 1382 3206 422 Fragments arranged by size: 3206 1382 422 BsaAI yAC'GTr Cuts at: 0 834 4609 5010 Size: 834 3775 401 Fragments arranged by size: 3775 834 401 BsaHI Gr'CG_yC Cuts at: 0 3677 5010 Size: 3677 1333 BsaWI w'CCGG_w Cuts at: 0 93 5010 Size: 93 4917 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 88 118 2986 3016 5010 Size: 88 30 2868 30 1994 Fragments arranged by size: 2868 1994 88 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 187 219 306 3010 5010 Size: 187 32 87 2704 2000 Fragments arranged by size: 2704 2000 187 87 32 BseYI C'CCAG_C Cuts at: 0 151 163 2793 5010 Size: 151 12 2630 2217 Fragments arranged by size: 2630 2217 151 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3241 5010 Size: 3241 1769 BsiEI CG_ry'CG Cuts at: 0 248 5010 Size: 248 4762 BsiHKAI G_wGCw'C Cuts at: 0 2401 3731 5010 Size: 2401 1330 1279 Fragments arranged by size: 2401 1330 1279 BsmI GAATG_Cn' Cuts at: 0 849 2775 3450 4149 5010 Size: 849 1926 675 699 861 Fragments arranged by size: 1926 861 849 699 675 BsmBI CGTCTCn'nnnn_ Cuts at: 0 10 62 4658 5010 Size: 10 52 4596 352 Fragments arranged by size: 4596 352 52 10 Bsp1286I G_dGCh'C Cuts at: 0 152 275 2401 2593 2962 3731 5010 Size: 152 123 2126 192 369 769 1279 Fragments arranged by size: 2126 1279 769 369 192 152 123 BspEI T'CCGG_A Cuts at: 0 93 5010 Size: 93 4917 BspHI T'CATG_A Cuts at: 0 3668 3708 5010 Size: 3668 40 1302 Fragments arranged by size: 3668 1302 40 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3844 5010 Size: 3844 1166 BsrDI GCAATG_nn' Cuts at: 0 707 2291 3848 4096 5010 Size: 707 1584 1557 248 914 Fragments arranged by size: 1584 1557 914 707 248 BssHII G'CGCG_C Cuts at: 0 2344 5010 Size: 2344 2666 BssSI C'ACGA_G Cuts at: 0 211 1136 5010 Size: 211 925 3874 Fragments arranged by size: 3874 925 211 BstAPI GCAn_nnn'nTGC Cuts at: 0 3006 5010 Size: 3006 2004 BstXI CCAn_nnnn'nTGG Cuts at: 0 118 2586 5010 Size: 118 2468 2424 Fragments arranged by size: 2468 2424 118 BstYI r'GATC_y Cuts at: 0 302 1021 2145 2619 5010 Size: 302 719 1124 474 2391 Fragments arranged by size: 2391 1124 719 474 302 Bsu36I CC'TnA_GG Cuts at: 0 221 2388 5010 Size: 221 2167 2622 Fragments arranged by size: 2622 2167 221 BtgI C'CryG_G Cuts at: 0 638 761 5010 Size: 638 123 4249 Fragments arranged by size: 4249 638 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 126 5010 Size: 126 4884 BtsI GCAGTG_nn' Cuts at: 0 3007 3041 5010 Size: 3007 34 1969 Fragments arranged by size: 3007 1969 34 ClaI AT'CG_AT Cuts at: 0 561 5010 Size: 561 4449 DraI TTT'AAA Cuts at: 0 723 1091 3890 4398 5010 Size: 723 368 2799 508 612 Fragments arranged by size: 2799 723 612 508 368 DrdI GACnn_nn'nnGTC Cuts at: 0 950 1455 5010 Size: 950 505 3555 Fragments arranged by size: 3555 950 505 EaeI y'GGCC_r Cuts at: 0 245 5010 Size: 245 4765 EagI C'GGCC_G Cuts at: 0 245 5010 Size: 245 4765 EarI CTCTTCn'nnn_ Cuts at: 0 263 307 486 2125 2207 2411 2720 3092 Size: 263 44 179 1639 82 204 309 372 Cuts at: 3092 3713 4505 4700 5010 Size: 621 792 195 310 Fragments arranged by size: 1639 792 621 372 310 309 263 204 195 179 82 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 80 5010 Size: 80 4930 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 335 520 1205 1847 1870 3491 3662 3995 Size: 335 185 685 642 23 1621 171 333 Cuts at: 3995 4058 4232 4258 4397 4724 4757 5010 Size: 63 174 26 139 327 33 253 Fragments arranged by size: 1621 685 642 335 333 327 253 185 174 171 139 63 33 26 23 EcoO109I rG'GnC_Cy Cuts at: 0 262 5010 Size: 262 4748 EcoRI G'AATT_C Cuts at: 0 1621 5010 Size: 1621 3389 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1102 1120 4456 5000 5010 Size: 1102 18 3336 544 10 Fragments arranged by size: 3336 1102 544 18 10 HaeII r_GCGC'y Cuts at: 0 91 164 1640 5010 Size: 91 73 1476 3370 Fragments arranged by size: 3370 1476 91 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 88 120 543 575 1182 1214 1416 1448 Size: 88 32 423 32 607 32 202 32 Cuts at: 1448 1596 1628 2536 2568 3421 3453 3507 3539 Size: 148 32 908 32 853 32 54 32 Cuts at: 3539 3968 4000 4289 4321 4462 4494 4504 4528 Size: 429 32 289 32 141 32 10 24 Cuts at: 4528 4536 4560 4633 4665 5010 Size: 8 24 73 32 345 Fragments arranged by size: 908 853 607 429 423 345 289 202 148 141 88 73 54 32 32 32 32 32 32 32 32 32 32 32 32 24 24 10 8 HincII GTy'rAC Cuts at: 0 2379 2981 4166 5010 Size: 2379 602 1185 844 Fragments arranged by size: 2379 1185 844 602 HindIII A'AGCT_T Cuts at: 0 2137 4932 5010 Size: 2137 2795 78 Fragments arranged by size: 2795 2137 78 MfeI C'AATT_G Cuts at: 0 4221 5010 Size: 4221 789 MslI CAynn'nnrTG Cuts at: 0 2207 2354 2438 4223 4330 4832 5010 Size: 2207 147 84 1785 107 502 178 Fragments arranged by size: 2207 1785 502 178 147 107 84 MspA1I CmG'CkG Cuts at: 0 155 2793 5010 Size: 155 2638 2217 Fragments arranged by size: 2638 2217 155 NcoI C'CATG_G Cuts at: 0 638 761 5010 Size: 638 123 4249 Fragments arranged by size: 4249 638 123 NdeI CA'TA_TG Cuts at: 0 580 957 1437 1731 3126 4869 5010 Size: 580 377 480 294 1395 1743 141 Fragments arranged by size: 1743 1395 580 480 377 294 141 NotI GC'GGCC_GC Cuts at: 0 245 5010 Size: 245 4765 NsiI A_TGCA'T Cuts at: 0 546 2361 5010 Size: 546 1815 2649 Fragments arranged by size: 2649 1815 546 NspI r_CATG'y Cuts at: 0 601 1474 1563 1810 3358 3499 5010 Size: 601 873 89 247 1548 141 1511 Fragments arranged by size: 1548 1511 873 601 247 141 89 PciI A'CATG_T Cuts at: 0 597 5010 Size: 597 4413 PflMI CCAn_nnn'nTGG Cuts at: 0 4450 5010 Size: 4450 560 PmlI CAC'GTG Cuts at: 0 834 4609 5010 Size: 834 3775 401 Fragments arranged by size: 3775 834 401 PshAI GACnn'nnGTC Cuts at: 0 2703 5010 Size: 2703 2307 PsiI TTA'TAA Cuts at: 0 1235 3625 5010 Size: 1235 2390 1385 Fragments arranged by size: 2390 1385 1235 PstI C_TGCA'G Cuts at: 0 3036 5010 Size: 3036 1974 PvuII CAG'CTG Cuts at: 0 2793 5010 Size: 2793 2217 SapI GCTCTTCn'nnn_ Cuts at: 0 263 307 2411 5010 Size: 263 44 2104 2599 Fragments arranged by size: 2599 2104 263 44 ScaI AGT'ACT Cuts at: 0 4071 5010 Size: 4071 939 SexAI A'CCwGG_T Cuts at: 0 566 2820 3534 5010 Size: 566 2254 714 1476 Fragments arranged by size: 2254 1476 714 566 SfcI C'TryA_G Cuts at: 0 432 3032 5010 Size: 432 2600 1978 Fragments arranged by size: 2600 1978 432 SmaI CCC'GGG Cuts at: 0 2890 3550 5010 Size: 2890 660 1460 Fragments arranged by size: 2890 1460 660 SmlI C'TyrA_G Cuts at: 0 100 359 1460 1971 2140 2256 3372 3400 Size: 100 259 1101 511 169 116 1116 28 Cuts at: 3400 4057 4468 4892 4962 5010 Size: 657 411 424 70 48 Fragments arranged by size: 1116 1101 657 511 424 411 259 169 116 100 70 48 28 SphI G_CATG'C Cuts at: 0 1563 1810 5010 Size: 1563 247 3200 Fragments arranged by size: 3200 1563 247 SspI AAT'ATT Cuts at: 0 384 5010 Size: 384 4626 StuI AGG'CCT Cuts at: 0 25 282 2872 5010 Size: 25 257 2590 2138 Fragments arranged by size: 2590 2138 257 25 StyI C'CwwG_G Cuts at: 0 638 761 3281 5010 Size: 638 123 2520 1729 Fragments arranged by size: 2520 1729 638 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 631 694 754 1406 2074 5010 Size: 631 63 60 652 668 2936 Fragments arranged by size: 2936 668 652 631 63 60 TatI w'GTAC_w Cuts at: 0 3071 3133 4069 5010 Size: 3071 62 936 941 Fragments arranged by size: 3071 941 936 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 584 616 2064 2096 2790 2822 2986 3018 Size: 584 32 1448 32 694 32 164 32 Cuts at: 3018 5010 Size: 1992 Fragments arranged by size: 1992 1448 694 584 164 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2374 5010 Size: 2374 2636 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3507 4082 5010 Size: 3507 575 928 Fragments arranged by size: 3507 928 575 XhoI C'TCGA_G Cuts at: 0 359 1460 5010 Size: 359 1101 3550 Fragments arranged by size: 3550 1101 359 XmaI C'CCGG_G Cuts at: 0 2888 3548 5010 Size: 2888 660 1462 Fragments arranged by size: 2888 1462 660 XmnI GAAnn'nnTTC Cuts at: 0 4001 4439 4909 5010 Size: 4001 438 470 101 Fragments arranged by size: 4001 470 438 101 ZraI GAC'GTC Cuts at: 0 3678 5010 Size: 3678 1332 Enzymes that do cut: AatII AccI AcuI AflII AflIII AhdI AleI ApoI AseI AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII MfeI MslI MspA1I NcoI NdeI NotI NsiI NspI PciI PflMI PmlI PshAI PsiI PstI PvuII SapI ScaI SexAI SfcI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AgeI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BmtI BsaBI BsiWI BsrBI BsrFI BsrGI BstBI BstEII BstZ17I CspCI DraIII EcoICRI EcoNI EcoRV FseI FspI FspAI HpaI KasI KpnI MluI MmeI MscI NaeI NarI NgoMIV NheI NruI PacI PasI PfoI PmeI PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI