Restriction map for
pFN21AE3898
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BsaBI BsaI BsgI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KspI MamI
MfeI MluI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 492 GACGTC GACGT^C
Acc65I 521 GGTACC G^GTACC
AccI 460 GTCGAC GT^[AC][GT]AC
AccII 720 CGCG CG^CG
AlwNI 82 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 521 GGTACC G^GTACC
AspHI 724 GAGCTC G[AT]GC[AT]^C
BalI 527 TGGCCA TGG^CCA
BamHI 774 GGATCC G^GATCC
BanII 724 GAGCTC G[GA]GC[CT]^C
BbvI 627 GCTGC GCAGC(8/12)
BcnI 713 CCGGG CC^[GC]GG
BlpI 369 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 369 GCTCAGC GC^T[ACGT]AGC
BsaAI 328 TACGTG [CT]AC^GT[GA]
BsiEI 512 CGGTCG CG[GA][CT]^CG
BsiHKAI 724 GAGCTC G[AT]GC[AT]^C
BstUI 720 CGCG CG^CG
BstXI 299 CCAAGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 774 GGATCC [GA]^GATC[CT]
Cac8I 46 GCGGGC GC[ACGT]^[ACGT]GC
CelII 369 GCTCAGC GC^T[ACGT]AGC
DrdI 739 GACTTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 228 CCATGG C^C[GA][CT]GG
EaeI 527 TGGCCA [CT]^GGCC[GA]
EcoNI 234 CCTAGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 228 CCATGG C^C[AT][AT]GG
HgaI 823 GACGC GACGC(5/10)
HincII 460 GTCGAC GT[CT]^[GA]AC
HindII 460 GTCGAC GT[CT]^[GA]AC
KpnI 521 GGTACC GGTAC^C
MflI 774 GGATCC [GA]^GATC[CT]
MluNI 527 TGGCCA TGG^CCA
MscI 527 TGGCCA TGG^CCA
MspA1I 82 CAGCGG C[AC]G^C[GT]G
MvnI 720 CGCG CG^CG
NciI 713 CCGGG CC^[GC]GG
NcoI 228 CCATGG C^CATGG
PflMI 735 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 724 GAGCTC GAGCT^C
SalI 460 GTCGAC G^TCGAC
SapI 262 GCTCTTC GCTCTTC(1/4)
StyI 228 CCATGG C^C[AT][AT]GG
TseI 627 GCTGC G^C[AT]GC
Van91I 735 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 267 TCTAGA T^CTAGA
XhoII 774 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 114 TCCGGA T^CCGGA
593 TCCGGA
AcyI 492 GACGTC G[GA]^CG[CT]C
823 GACGCC
AfaI 327 GTAC GT^AC
522 GTAC
AluI 626 AGCT AG^CT
725 AGCT
AlwI 775 GATCC GGATC(4/5)
774 GGATC
AvaII 403 GGACC G^G[AT]CC
747 GGTCC
BanI 9 GGTGCC G^G[CT][GA]CC
521 GGTACC
BbsI 150 GAAGAC GAAGAC(2/6)
334 GAAGAC
BclI 422 TGATCA T^GATCA
554 TGATCA
BfaI 235 CTAG C^TAG
268 CTAG
BmyI 724 GAGCTC G[AGT]GC[ACT]^C
766 GTGCCC
BpuAI 150 GAAGAC GAAGAC(2/6)
334 GAAGAC
BsaHI 492 GACGTC G[GA]^CG[CT]C
823 GACGCC
BseAI 114 TCCGGA T^CCGGA
593 TCCGGA
BseRI 754 CTCCTC GAGGAG(10/8)
4 GAGGAG
Bsp1286I 724 GAGCTC G[AGT]GC[ACT]^C
766 GTGCCC
BspEI 114 TCCGGA T^CCGGA
593 TCCGGA
BsrBI 778 CCGCTC CCGCTC(-3/-3)
101 GAGCGG
BsrI 449 ACTGG ACTGG(1/-1)
807 CCAGT
DraII 402 GGGACCC [GA]G^G[ACGT]CC[CT]
746 AGGTCCC
EarI 263 CTCTTC CTCTTC(1/4)
907 GAAGAG
EcoO109I 402 GGGACCC [GA]G^G[ACGT]CC[CT]
746 AGGTCCC
FauI 46 GCGGG CCCGC(4/6)
103 GCGGG
FbaI 422 TGATCA T^GATCA
554 TGATCA
HaeII 37 AGCGCC [GA]GCGC^[CT]
214 AGCGCC
Hin1I 492 GACGTC G[GA]^CG[CT]C
823 GACGCC
Ksp632I 263 CTCTTC CTCTTC(1/4)
907 GAAGAG
MaeI 235 CTAG C^TAG
268 CTAG
MaeIII 579 GTCAC ^GT[ACGT]AC
797 GTGAC
MroI 114 TCCGGA T^CCGGA
593 TCCGGA
MslI 538 CACATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
636 CATCGGGGTG
PpuMI 402 GGGACCC [GA]G^G[AT]CC[CT]
746 AGGTCCC
RsaI 327 GTAC GT^AC
522 GTAC
SfaNI 71 GATGC GCATC(5/9)
385 GATGC
SfcI 125 CTATAG C^T[GA][CT]AG
852 CTACAG
Tsp45I 579 GTCAC ^GT[GC]AC
797 GTGAC
Tsp509I 793 AATT ^AATT
864 AATT
TspRI 356 CAGTG CA[GC]TG[ACGT][ACGT]^
551 CAGTG
XcmI 219 CCACCTTGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
632 CCATCATCGGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI BpmI BsaJI BsaWI BsiYI
BslI BsmFI BspDI BssKI BstF5I
BstNI CfoI Cfr13I ClaI DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TaqI TfiI TthHB8I