################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI HindIII HinfI HpaI KpnI KspI MaeII MamI MfeI MluI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 980 CGCG CG^CG AccIII 527 TCCGGA T^CCGGA AcyI 268 GGCGCC G[GA]^CG[CT]C AfaI 676 GTAC GT^AC Alw44I 211 GTGCAC G^TGCAC AocI 621 CCTGAGG CC^T[ACGT]AGG ApaLI 211 GTGCAC G^TGCAC AspHI 211 GTGCAC G[AT]GC[AT]^C AviII 569 TGCGCA TGC^GCA AvrII 846 CCTAGG C^CTAGG BbeI 268 GGCGCC GGCGC^C BglII 687 AGATCT A^GATCT BlnI 846 CCTAGG C^CTAGG BsaHI 268 GGCGCC G[GA]^CG[CT]C BsaWI 527 TCCGGA [AT]^CCGG[AT] BseAI 527 TCCGGA T^CCGGA BsiHKAI 211 GTGCAC G[AT]GC[AT]^C BsmI 41 GCATTC GAATGC(1/-1) BspEI 527 TCCGGA T^CCGGA BsrBI 138 CCGCTC CCGCTC(-3/-3) BsrDI 302 GCAATG GCAATG(2/0) BsrFI 757 GCCGGT [GA]^CCGG[CT] BssSI 471 CTCGTG CACGAG(-5/-1) BstAPI 754 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 105 GGTCACC G^GT[ACGT]ACC BstPI 105 GGTCACC G^GT[ACGT]ACC BstUI 980 CGCG CG^CG Bsu36I 621 CCTGAGG CC^T[ACGT]AGG Cfr10I 757 GCCGGT [GA]^CCGG[CT] Eco81I 621 CCTGAGG CC^T[ACGT]AGG EcoO65I 105 GGTCACC G^GT[ACGT]ACC FauI 870 GCGGG CCCGC(4/6) FspI 569 TGCGCA TGC^GCA HaeII 268 GGCGCC [GA]GCGC^[CT] HgaI 1014 GACGC GACGC(5/10) Hin1I 268 GGCGCC G[GA]^CG[CT]C HincII 167 GTCAAC GT[CT]^[GA]AC HindII 167 GTCAAC GT[CT]^[GA]AC KasI 268 GGCGCC G^GCGCC MroI 527 TCCGGA T^CCGGA MvnI 980 CGCG CG^CG NarI 268 GGCGCC GG^CGCC NcoI 147 CCATGG C^CATGG PpuMI 458 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 676 GTAC GT^AC ScaI 675 AGTACT AGT^ACT SfoI 268 GGCGCC GGC^GCC SmaI 541 CCCGGG CCC^GGG StuI 410 AGGCCT AGG^CCT TaqI 881 TCGA T^CGA TthHB8I 881 TCGA T^CGA XmaCI 541 CCCGGG C^CCGGG XmaI 541 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 226 GAATTC [GA]^AATT[CT] 328 GAATTC AlwI 363 GGATC GGATC(4/5) 902 GGATC AlwNI 769 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 913 CAGAACCTG ApoI 226 GAATTC [GA]^AATT[CT] 328 GAATTC BanI 268 GGCGCC G^G[CT][GA]CC 760 GGTGCC BanII 114 GAGCCC G[GA]GC[CT]^C 832 GGGCTC BsaI 601 GAGACC GGTCTC(1/5) 468 GGTCTC BseRI 259 CTCCTC GAGGAG(10/8) 691 CTCCTC BsmAI 601 GAGAC GTCTC(1/5) 469 GTCTC BsmFI 863 GGGAC GGGAC(10/14) 1035 GTCCC BstF5I 842 CATCC GGATG(2/0) 873 GGATG DraII 458 AGGTCCT [GA]G^G[ACGT]CC[CT] 770 AGGGCCT DsaI 147 CCATGG C^C[GA][CT]GG 221 CCGTGG EarI 403 CTCTTC CTCTTC(1/4) 721 CTCTTC EcoNI 258 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 402 CCTCTTCCAGG EcoO109I 458 AGGTCCT [GA]G^G[ACGT]CC[CT] 770 AGGGCCT EcoRI 226 GAATTC G^AATTC 328 GAATTC FokI 842 CATCC GGATG(9/13) 873 GGATG HphI 107 TCACC GGTGA(8/7) 560 TCACC Ksp632I 403 CTCTTC CTCTTC(1/4) 721 CTCTTC MaeIII 106 GTCAC ^GT[ACGT]AC 908 GTGAC NspI 788 ACATGC [GA]CATG^[CT] 866 ACATGC SfaNI 501 GCATC GCATC(5/9) 841 GCATC Tsp45I 106 GTCAC ^GT[GC]AC 908 GTGAC XbaI 58 TCTAGA T^CTAGA 654 TCTAGA XcmI 299 CCAGCAATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 803 CCAATAACTCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AluI AvaI AvaII BalI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiYI BslI BsoBI Bsp1286I BspMI BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeI MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI StyI TseI Tsp509I TspRI XhoII