(Circular) (Six-base) MAPSORT of: pFN21AE2154.seq Check: 8763 from: 1 to: 5746 pFN21AE2154 5746 bp With 182 enzymes: SgfI * May 20, 2016 17:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2188 2259 2188 Size: 71 5675 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2930 3021 3809 5193 5461 1632 Size: 1298 91 788 1384 268 1917 Fragments arranged by size: 1917 1384 1298 788 268 91 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2690 1184 Size: 1506 4240 AlwNI CAG_nnn'CTG Cuts at: 2779 2923 3489 5061 2779 Size: 144 566 1572 3464 Fragments arranged by size: 3464 1572 566 144 ApaLI G'TGCA_C Cuts at: 2216 3803 4959 2216 Size: 1587 1156 3003 Fragments arranged by size: 3003 1587 1156 ApoI r'AATT_y Cuts at: 1493 2231 2333 3055 3281 3433 5542 1493 Size: 738 102 722 226 152 2109 1697 Fragments arranged by size: 2109 1697 738 722 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2401 2546 2831 3071 1870 Size: 73 458 145 285 240 4545 Fragments arranged by size: 4545 458 285 240 145 73 AvrII C'CTAG_G Cuts at: 2851 2851 Size: 5746 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2273 2765 3067 4522 618 Size: 325 217 627 486 492 302 1455 1842 Fragments arranged by size: 1842 1455 627 492 486 325 302 217 BanII G_rGCy'C Cuts at: 1391 2123 2841 3065 1391 Size: 732 718 224 4072 Fragments arranged by size: 4072 732 718 224 BbeI G_GCGC'C Cuts at: 2277 2277 Size: 5746 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4775 BbvCI CC'TCA_GC Cuts at: 2098 2357 2419 2098 Size: 259 62 5425 Fragments arranged by size: 5425 259 62 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 1548 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2043 2548 2756 Size: 107 122 71 631 605 371 505 208 Cuts at: 2756 2836 4357 136 Size: 80 1521 1525 Fragments arranged by size: 1525 1521 631 605 505 371 208 122 107 80 71 BglII A'GATC_T Cuts at: 2692 5741 2692 Size: 3049 2697 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2220 2832 3807 4963 1790 Size: 430 612 975 1156 2573 Fragments arranged by size: 2573 1156 975 612 430 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2693 2875 3477 Size: 416 53 83 399 24 1276 182 602 Cuts at: 3477 4435 442 Size: 958 1753 Fragments arranged by size: 1753 1276 958 602 416 399 182 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2287 2480 2489 4406 1239 Size: 1013 35 193 9 1917 2579 Fragments arranged by size: 2579 1917 1013 193 35 9 Bpu10I CC'TnA_GC Cuts at: 2098 2117 2357 2419 2924 2098 Size: 19 240 62 505 4920 Fragments arranged by size: 4920 505 240 62 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2479 2600 4409 882 Size: 617 980 121 1809 2219 Fragments arranged by size: 2219 1809 980 617 121 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2274 3553 3935 4570 Size: 53 83 186 936 741 1279 382 635 Cuts at: 4570 275 Size: 1451 Fragments arranged by size: 1451 1279 936 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2532 4179 4577 4851 4998 5409 2532 Size: 1647 398 274 147 411 2869 Fragments arranged by size: 2869 1647 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 2585 2607 2615 Size: 30 165 30 1059 30 93 22 8 Cuts at: 2615 2637 1178 Size: 22 4287 Fragments arranged by size: 4287 1059 165 93 30 30 30 22 22 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2687 1186 Size: 1069 432 4245 Fragments arranged by size: 4245 1069 432 BseYI C'CCAG_C Cuts at: 2993 4949 5445 2993 Size: 1956 496 3294 Fragments arranged by size: 3294 1956 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 3807 3892 4963 2220 Size: 1587 85 1071 3003 Fragments arranged by size: 3003 1587 1071 85 BsmI GAATG_Cn' Cuts at: 2046 3257 3350 2046 Size: 1211 93 4442 Fragments arranged by size: 4442 1211 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2220 2832 2841 3065 3807 3892 Size: 399 333 97 612 9 224 742 85 Cuts at: 3892 4963 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 612 399 333 224 97 85 9 BspEI T'CCGG_A Cuts at: 2532 5409 2532 Size: 2877 2869 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2188 2208 2259 2724 3101 844 Size: 1344 20 51 465 377 3489 Fragments arranged by size: 3489 1344 465 377 51 20 BsrBI CCG'CTC Cuts at: 1394 1823 2145 3633 1394 Size: 429 322 1488 3507 Fragments arranged by size: 3507 1488 429 322 BsrDI GCAATG_nn' Cuts at: 65 1248 2314 4241 4415 65 Size: 1183 1066 1927 174 1396 Fragments arranged by size: 1927 1396 1183 1066 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2762 4390 4577 1700 Size: 110 952 1628 187 2869 Fragments arranged by size: 2869 1628 952 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 2476 3806 4818 2476 Size: 1330 1012 3404 Fragments arranged by size: 3404 1330 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2765 794 Size: 1971 3775 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2110 1184 Size: 926 4820 BstYI r'GATC_y Cuts at: 1972 2368 2692 2907 3076 3443 3828 3845 5297 Size: 396 324 215 169 367 385 17 1452 Cuts at: 5297 5383 5395 5741 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 396 385 367 346 324 215 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2627 2627 Size: 5746 BtgI C'CryG_G Cuts at: 513 1065 2152 2226 513 Size: 552 1087 74 4033 Fragments arranged by size: 4033 1087 552 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 3036 3262 4056 4076 3036 Size: 226 794 20 4706 Fragments arranged by size: 4706 794 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2342 2556 Size: 54 1425 50 275 18 393 119 214 Cuts at: 2556 2748 2820 3033 3193 4082 5711 8 Size: 192 72 213 160 889 1629 43 Fragments arranged by size: 1629 1425 889 393 275 214 213 192 160 119 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2414 2732 3676 1353 Size: 1061 318 944 3423 Fragments arranged by size: 3423 1061 944 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2287 2480 2489 2930 3021 3809 Size: 393 620 35 193 9 441 91 788 Cuts at: 3809 4406 5193 5461 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 788 787 620 597 441 393 268 193 91 35 9 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2411 2267 Size: 144 5602 EcoO109I rG'GnC_Cy Cuts at: 2464 2776 2464 Size: 312 5434 EcoRI G'AATT_C Cuts at: 2231 2333 3055 3433 2231 Size: 102 722 378 4544 Fragments arranged by size: 4544 722 378 102 FspI TGC'GCA Cuts at: 2576 4252 2576 Size: 1676 4070 HaeII r_GCGC'y Cuts at: 1678 2277 4893 1678 Size: 599 2616 2531 Fragments arranged by size: 2616 2531 599 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4387 4419 4461 4493 1373 Size: 32 135 32 2815 32 42 32 2626 Fragments arranged by size: 2815 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2174 3090 3336 669 Size: 988 281 236 916 246 3079 Fragments arranged by size: 3079 988 916 281 246 236 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KasI G'GCGC_C Cuts at: 2273 2273 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2583 4860 5044 1305 Size: 1278 2277 184 2007 Fragments arranged by size: 2277 2007 1278 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2558 2750 2822 3035 10 Size: 54 1425 343 726 192 72 213 2721 Fragments arranged by size: 2721 1425 726 343 213 192 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 3704 4063 4222 518 Size: 648 2538 359 159 2042 Fragments arranged by size: 2538 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2356 2458 2638 2788 3162 3839 4987 5232 Size: 750 102 180 150 374 677 1148 245 Cuts at: 5232 1606 Size: 2120 Fragments arranged by size: 2120 1148 750 677 374 245 180 150 102 NarI GG'CG_CC Cuts at: 2274 2274 Size: 5746 NcoI C'CATG_G Cuts at: 513 1065 2152 513 Size: 552 1087 4107 Fragments arranged by size: 4107 1087 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2797 2875 3104 4649 1132 Size: 97 1568 78 229 1545 2229 Fragments arranged by size: 2229 1568 1545 229 97 78 PasI CC'CwG_GG Cuts at: 1348 2039 1348 Size: 691 5055 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 2466 2466 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2464 2464 Size: 5746 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2356 2458 2788 2356 Size: 102 330 5314 Fragments arranged by size: 5314 330 102 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 2682 3994 5706 2682 Size: 1312 1712 2722 Fragments arranged by size: 2722 1712 1312 SfcI C'TryA_G Cuts at: 826 1045 3094 4229 4910 5101 826 Size: 219 2049 1135 681 191 1471 Fragments arranged by size: 2049 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2275 2275 Size: 5746 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 2548 3073 1872 Size: 676 525 4545 Fragments arranged by size: 4545 676 525 SmlI C'TyrA_G Cuts at: 820 1017 1943 3850 4751 5013 820 Size: 197 926 1907 901 262 1553 Fragments arranged by size: 1907 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 2417 1517 Size: 900 4846 StyI C'CwwG_G Cuts at: 513 1065 2152 2587 2851 3185 513 Size: 552 1087 435 264 334 3074 Fragments arranged by size: 3074 1087 552 435 334 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3773 3790 3943 4128 4573 325 Size: 1255 2193 17 153 185 445 1498 Fragments arranged by size: 2193 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2680 3992 5704 Size: 274 80 33 51 1443 703 1312 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1712 1443 1312 703 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2462 2494 2607 2639 5706 5738 2462 Size: 32 113 32 3067 32 2470 Fragments arranged by size: 3067 2470 113 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 2063 2659 3082 2063 Size: 596 423 4727 Fragments arranged by size: 4727 596 423 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2815 2311 Size: 504 5242 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 2546 3071 1870 Size: 676 525 4545 Fragments arranged by size: 4545 676 525 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI