Restriction map for
pFN21AE2154
(Linear) (Six-base) MAPSORT of: KIEE2154.seq Check: 4770 from: 1 to: 1041
KIEE2154.orf
With 182 enzymes: SgfI *
May 20, 2016 17:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 183 254 1041
Size: 183 71 787
Fragments arranged by size:
787 183 71
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1016 1041
Size: 925 91 25
Fragments arranged by size:
925 91 25
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 685 1041
Size: 685 356
AlwNI CAG_nnn'CTG
Cuts at: 0 774 918 1041
Size: 774 144 123
Fragments arranged by size:
774 144 123
ApaLI G'TGCA_C
Cuts at: 0 211 1041
Size: 211 830
ApoI r'AATT_y
Cuts at: 0 226 328 1041
Size: 226 102 713
Fragments arranged by size:
713 226 102
AvaI C'yCGr_G
Cuts at: 0 396 541 826 1041
Size: 396 145 285 215
Fragments arranged by size:
396 285 215 145
AvrII C'CTAG_G
Cuts at: 0 846 1041
Size: 846 195
BanI G'GyrC_C
Cuts at: 0 268 760 1041
Size: 268 492 281
Fragments arranged by size:
492 281 268
BanII G_rGCy'C
Cuts at: 0 118 836 1041
Size: 118 718 205
Fragments arranged by size:
718 205 118
BbeI G_GCGC'C
Cuts at: 0 272 1041
Size: 272 769
BbvCI CC'TCA_GC
Cuts at: 0 93 352 414 1041
Size: 93 259 62 627
Fragments arranged by size:
627 259 93 62
BglI GCCn_nnn'nGGC
Cuts at: 0 38 543 751 831 1041
Size: 38 505 208 80 210
Fragments arranged by size:
505 210 208 80 38
BglII A'GATC_T
Cuts at: 0 687 1041
Size: 687 354
Bme1580I G_kGCm'C
Cuts at: 0 215 827 1041
Size: 215 612 214
Fragments arranged by size:
612 215 214
BmrI ACTGGGnnnn_n'
Cuts at: 0 688 870 1041
Size: 688 182 171
Fragments arranged by size:
688 182 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 282 475 484 1041
Size: 247 35 193 9 557
Fragments arranged by size:
557 247 193 35 9
Bpu10I CC'TnA_GC
Cuts at: 0 93 112 352 414 919 1041
Size: 93 19 240 62 505 122
Fragments arranged by size:
505 240 122 93 62 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 474 595 1041
Size: 474 121 446
Fragments arranged by size:
474 446 121
BsaHI Gr'CG_yC
Cuts at: 0 269 1041
Size: 269 772
BsaWI w'CCGG_w
Cuts at: 0 527 1041
Size: 527 514
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 580 602 610 632 1041
Size: 457 30 93 22 8 22 409
Fragments arranged by size:
457 409 93 30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 682 1041
Size: 250 432 359
Fragments arranged by size:
432 359 250
BseYI C'CCAG_C
Cuts at: 0 988 1041
Size: 988 53
BsiHKAI G_wGCw'C
Cuts at: 0 215 1041
Size: 215 826
BsmI GAATG_Cn'
Cuts at: 0 41 1041
Size: 41 1000
Bsp1286I G_dGCh'C
Cuts at: 0 118 215 827 836 1041
Size: 118 97 612 9 205
Fragments arranged by size:
612 205 118 97 9
BspEI T'CCGG_A
Cuts at: 0 527 1041
Size: 527 514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 183 203 254 719 1041
Size: 183 20 51 465 322
Fragments arranged by size:
465 322 183 51 20
BsrBI CCG'CTC
Cuts at: 0 140 1041
Size: 140 901
BsrDI GCAATG_nn'
Cuts at: 0 309 1041
Size: 309 732
BsrFI r'CCGG_y
Cuts at: 0 757 1041
Size: 757 284
BssSI C'ACGA_G
Cuts at: 0 471 1041
Size: 471 570
BstAPI GCAn_nnn'nTGC
Cuts at: 0 760 1041
Size: 760 281
BstEII G'GTnAC_C
Cuts at: 0 105 1041
Size: 105 936
BstYI r'GATC_y
Cuts at: 0 363 687 902 1041
Size: 363 324 215 139
Fragments arranged by size:
363 324 215 139
Bsu36I CC'TnA_GG
Cuts at: 0 622 1041
Size: 622 419
BtgI C'CryG_G
Cuts at: 0 147 221 1041
Size: 147 74 820
Fragments arranged by size:
820 147 74
BtsI GCAGTG_nn'
Cuts at: 0 1031 1041
Size: 1031 10
EaeI y'GGCC_r
Cuts at: 0 218 337 551 743 815 1028 1041
Size: 218 119 214 192 72 213 13
Fragments arranged by size:
218 214 213 192 119 72 13
EarI CTCTTCn'nnn_
Cuts at: 0 409 727 1041
Size: 409 318 314
Fragments arranged by size:
409 318 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 282 475 484 925 1016 1041
Size: 247 35 193 9 441 91 25
Fragments arranged by size:
441 247 193 91 35 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 406 1041
Size: 262 144 635
Fragments arranged by size:
635 262 144
EcoO109I rG'GnC_Cy
Cuts at: 0 459 771 1041
Size: 459 312 270
Fragments arranged by size:
459 312 270
EcoRI G'AATT_C
Cuts at: 0 226 328 1041
Size: 226 102 713
Fragments arranged by size:
713 226 102
FspI TGC'GCA
Cuts at: 0 571 1041
Size: 571 470
HaeII r_GCGC'y
Cuts at: 0 272 1041
Size: 272 769
HincII GTy'rAC
Cuts at: 0 169 1041
Size: 169 872
KasI G'GCGC_C
Cuts at: 0 268 1041
Size: 268 773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1041
Size: 578 463
MscI TGG'CCA
Cuts at: 0 553 745 817 1030 1041
Size: 553 192 72 213 11
Fragments arranged by size:
553 213 192 72 11
MspA1I CmG'CkG
Cuts at: 0 351 453 633 783 1041
Size: 351 102 180 150 258
Fragments arranged by size:
351 258 180 150 102
NarI GG'CG_CC
Cuts at: 0 269 1041
Size: 269 772
NcoI C'CATG_G
Cuts at: 0 147 1041
Size: 147 894
NspI r_CATG'y
Cuts at: 0 792 870 1041
Size: 792 78 171
Fragments arranged by size:
792 171 78
PasI CC'CwG_GG
Cuts at: 0 34 1041
Size: 34 1007
PfoI T'CCnGG_A
Cuts at: 0 461 1041
Size: 461 580
PpuMI rG'GwC_Cy
Cuts at: 0 459 1041
Size: 459 582
PvuII CAG'CTG
Cuts at: 0 351 453 783 1041
Size: 351 102 330 258
Fragments arranged by size:
351 330 258 102
ScaI AGT'ACT
Cuts at: 0 677 1041
Size: 677 364
SfoI GGC'GCC
Cuts at: 0 270 1041
Size: 270 771
SmaI CCC'GGG
Cuts at: 0 543 1041
Size: 543 498
StuI AGG'CCT
Cuts at: 0 412 1041
Size: 412 629
StyI C'CwwG_G
Cuts at: 0 147 582 846 1041
Size: 147 435 264 195
Fragments arranged by size:
435 264 195 147
TatI w'GTAC_w
Cuts at: 0 675 1041
Size: 675 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 457 489 602 634 1041
Size: 457 32 113 32 407
Fragments arranged by size:
457 407 113 32 32
XbaI T'CTAG_A
Cuts at: 0 58 654 1041
Size: 58 596 387
Fragments arranged by size:
596 387 58
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 810 1041
Size: 306 504 231
Fragments arranged by size:
504 306 231
XmaI C'CCGG_G
Cuts at: 0 541 1041
Size: 541 500
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaLI ApoI AvaI AvrII
BanI BanII BbeI BbvCI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII HincII
KasI MmeI MscI MspA1I NarI NcoI NspI PasI
PfoI PpuMI PvuII ScaI SfoI SmaI StuI StyI
TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BspHI BsrGI BssHII BstBI BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfcI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XhoI XmnI ZraI