################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbvCI BciVI BfaI BfrI BlnI BsaBI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MscI MseI MunI NarI NcoI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI Sse8387I SspI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 622 GACGTC GACGT^C AccIII 1265 TCCGGA T^CCGGA AflIII 203 ACGTGT A^C[GA][CT]GT BanI 1420 GGCACC G^G[CT][GA]CC BbrPI 475 CACGTG CAC^GTG BbsI 766 GAAGAC GAAGAC(2/6) BcgI 1295 GCAGCGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1589 AGATCT A^GATCT BpuAI 766 GAAGAC GAAGAC(2/6) BsaAI 475 CACGTG [CT]AC^GT[GA] BsaWI 1265 TCCGGA [AT]^CCGG[AT] BseAI 1265 TCCGGA T^CCGGA BspEI 1265 TCCGGA T^CCGGA BsrI 1457 ACTGG ACTGG(1/-1) BssHII 793 GCGCGC G^CGCGC BstAPI 738 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 723 GGTCACC G^GT[ACGT]ACC BstPI 723 GGTCACC G^GT[ACGT]ACC BstXI 1424 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1589 AGATCT [GA]^GATC[CT] DraIII 457 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 723 GGTCACC G^GT[ACGT]ACC MflI 1589 AGATCT [GA]^GATC[CT] MroI 1265 TCCGGA T^CCGGA NotI 629 GCGGCCGC GC^GGCCGC NspI 1036 GCATGC [GA]CATG^[CT] PaeR7I 1375 CTCGAG C^TCGAG PflMI 116 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 475 CACGTG CAC^GTG PmlI 475 CACGTG CAC^GTG PstI 1141 CTGCAG CTGCA^G SgrAI 1350 CGCCGGCG C[GA]^CCGG[CT]G SphI 1036 GCATGC GCATG^C TaqI 1376 TCGA T^CGA TthHB8I 1376 TCGA T^CGA Van91I 116 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 116 CCAACCTGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1375 CTCGAG C^TCGAG XhoII 1589 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### ApaI 921 GGGCCC GGGCC^C 1095 GGGCCC AvaII 165 GGACC G^G[AT]CC 840 GGTCC BclI 686 TGATCA T^GATCA 699 TGATCA BsaI 514 GAGACC GGTCTC(1/5) 1024 GAGACC BsmAI 514 GAGAC GTCTC(1/5) 1024 GAGAC Bsp1407I 563 TGTACA T^GTACA 779 TGTACA BsrGI 563 TGTACA T^GTACA 779 TGTACA EagI 630 CGGCCG C^GGCCG 887 CGGCCG EclXI 630 CGGCCG C^GGCCG 887 CGGCCG Eco52I 630 CGGCCG C^GGCCG 887 CGGCCG FbaI 686 TGATCA T^GATCA 699 TGATCA HaeII 1278 GGCGCT [GA]GCGC^[CT] 1371 GGCGCT HinfI 666 GAGTC G^A[ACGT]TC 1406 GACTC KspI 1014 CCGCGG CCGC^GG 1056 CCGCGG MboII 766 GAAGA GAAGA(8/7) 1587 GAAGA MslI 198 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1323 CACGGAGGTG PleI 1406 GACTC GAGTC(4/5) 666 GAGTC SacII 1014 CCGCGG CCGC^GG 1056 CCGCGG SfcI 1128 CTACAG C^T[GA][CT]AG 1141 CTGCAG SmaI 87 CCCGGG CCC^GGG 501 CCCGGG SspBI 563 TGTACA T^GTACA 779 TGTACA XmaCI 87 CCCGGG C^CCGGG 501 CCCGGG XmaI 87 CCCGGG C^CCGGG 501 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StuI TseI Tsp45I TspRI