Restriction map for
pFN21AE2620
(Circular) (Six-base) MAPSORT of: pFN21AE2620.seq Check: 2575 from: 1 to: 6349
pFN21AE2620 6349 bp
With 182 enzymes: SgfI *
May 9, 2016 12:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2393 2798 2393
Size: 405 5944
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2631 4159 278
Size: 53 83 186 936 1095 1528 2468
Fragments arranged by size:
2468 1528 1095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4412 5796 6064 1632
Size: 2780 1384 268 1917
Fragments arranged by size:
2780 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 2208 5248 6225 1203
Size: 1005 3040 977 1327
Fragments arranged by size:
3040 1327 1005 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 3332 1166
Size: 2166 4183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2498 3072 1184
Size: 1314 574 4461
Fragments arranged by size:
4461 1314 574
AlwNI CAG_nnn'CTG
Cuts at: 4092 5664 4092
Size: 1572 4777
ApaI G_GGCC'C
Cuts at: 2930 3104 2930
Size: 174 6175
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 3658 3884 4036 6145 1493
Size: 2165 226 152 2109 1697
Fragments arranged by size:
2165 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2092 2506 3347 3380 3674 1870
Size: 73 113 36 414 841 33 294 4545
Fragments arranged by size:
4545 841 414 294 113 73 36 33
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3425 3670 5125 618
Size: 325 217 627 1638 245 1455 1842
Fragments arranged by size:
1842 1638 1455 627 325 245 217
BanII G_rGCy'C
Cuts at: 1391 2312 2376 2930 3104 3348 3668 1391
Size: 921 64 554 174 244 320 4072
Fragments arranged by size:
4072 921 554 320 244 174 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2778 928
Size: 971 879 4499
Fragments arranged by size:
4499 971 879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3289 3323 4540 4574 1903
Size: 34 50 34 1268 34 1217 34 3678
Fragments arranged by size:
3678 1268 1217 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 2691 2704 1548
Size: 1143 13 5193
Fragments arranged by size:
5193 1143 13
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2094 2448 3374
Size: 107 122 71 631 605 422 354 926
Cuts at: 3374 4960 136
Size: 1586 1525
Fragments arranged by size:
1586 1525 926 631 605 422 354 122
107 71
BglII A'GATC_T
Cuts at: 3594 6344 3594
Size: 2750 3599
BlpI GC'TnA_GC
Cuts at: 2114 2486 3287 3766 2114
Size: 372 801 479 4697
Fragments arranged by size:
4697 801 479 372
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2300 2472 2930 3061 3104 3428 4410
Size: 315 195 172 458 131 43 324 982
Cuts at: 4410 5566 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 982 458 324 315 195 172
131 43
BmgBI CAC'GTC
Cuts at: 1318 3388 1318
Size: 2070 4279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3472 4080 5038
Size: 416 53 83 399 24 2055 608 958
Cuts at: 5038 442
Size: 1753
Fragments arranged by size:
2055 1753 958 608 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3359 3458 3629 5009 1239
Size: 2120 99 171 1380 2579
Fragments arranged by size:
2579 2120 1380 171 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3377 3398 4474 5339 5637 3377
Size: 21 1076 865 298 4089
Fragments arranged by size:
4089 1076 865 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2513 3023 5012 882
Size: 617 1014 510 1989 2219
Fragments arranged by size:
2219 1989 1014 617 510
BsaAI yAC'GTr
Cuts at: 493 1204 2482 4140 493
Size: 711 1278 1658 2702
Fragments arranged by size:
2702 1658 1278 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 2430 2628 2775
Size: 53 83 186 936 603 294 198 147
Cuts at: 2775 3075 3549 4156 4538 5173 275
Size: 300 474 607 382 635 1451
Fragments arranged by size:
1451 936 635 607 603 474 382 300
294 198 186 147 83 53
BsaWI w'CCGG_w
Cuts at: 3270 4782 5180 5454 5601 6012 3270
Size: 1512 398 274 147 411 3607
Fragments arranged by size:
3607 1512 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2007 2037 1178
Size: 30 165 30 604 30 5490
Fragments arranged by size:
5490 604 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2033 3356 1186
Size: 844 3 1323 4179
Fragments arranged by size:
4179 1323 844 3
BseYI C'CCAG_C
Cuts at: 2080 2254 2375 2498 2810 2876 2884 3114 3126
Size: 174 121 123 312 66 8 230 12
Cuts at: 3126 5552 6048 2080
Size: 2426 496 2381
Fragments arranged by size:
2426 2381 496 312 230 174 123 121
66 12 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2438 2615 2776 2905 2933 3116
Size: 573 468 234 177 161 129 28 183
Cuts at: 3116 1163
Size: 4396
Fragments arranged by size:
4396 573 468 234 183 177 161 129
28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2359 2638 2895 3799 4560 5588
Size: 275 186 358 279 257 904 761 1028
Cuts at: 5588 1540
Size: 2301
Fragments arranged by size:
2301 1028 904 761 358 279 275 257
186
BsiHKAI G_wGCw'C
Cuts at: 2147 2487 2754 3393 4410 4495 5566 2147
Size: 340 267 639 1017 85 1071 2930
Fragments arranged by size:
2930 1071 1017 639 340 267 85
BsmI GAATG_Cn'
Cuts at: 3860 3953 3860
Size: 93 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2147 2300 2312 2376 2472 2487
Size: 399 315 42 153 12 64 96 15
Cuts at: 2487 2754 2930 3061 3104 3348 3393 3428 3668
Size: 267 176 131 43 244 45 35 240
Cuts at: 3668 4410 4495 5566 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 315 267 244 240
176 153 131 96 85 64 45 43
42 35 15 12
BspEI T'CCGG_A
Cuts at: 3270 6012 3270
Size: 2742 3607
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2393 2587 2798 3704 844
Size: 1549 194 211 906 3489
Fragments arranged by size:
3489 1549 906 211 194
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4844 5018 65
Size: 1183 3596 174 1396
Fragments arranged by size:
3596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2777 2857 3356 3551 4993 5180 1700
Size: 110 967 80 499 195 1442 187 2869
Fragments arranged by size:
2869 1442 967 499 195 187 110 80
BsrGI T'GTAC_A
Cuts at: 96 2568 2784 96
Size: 2472 216 3661
Fragments arranged by size:
3661 2472 216
BssHII G'CGCG_C
Cuts at: 1925 2798 1925
Size: 873 5476
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2749 794
Size: 1955 4394
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 2728 1184
Size: 1544 4805
BstXI CCAn_nnnn'nTGG
Cuts at: 3436 3436
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 3594 3679 4046 4431 4448 5900 5986 5998
Size: 1622 85 367 385 17 1452 86 12
Cuts at: 5998 6344 1972
Size: 346 1977
Fragments arranged by size:
1977 1622 1452 385 367 346 86 85
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2998 3019 3061 513
Size: 552 1933 21 42 3801
Fragments arranged by size:
3801 1933 552 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3349 3543 487
Size: 683 56 2123 194 3293
Fragments arranged by size:
3293 2123 683 194 56
BtsI GCAGTG_nn'
Cuts at: 2523 2620 3158 3865 4659 4679 2523
Size: 97 538 707 794 20 4193
Fragments arranged by size:
4193 794 707 538 97 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2194 2229 529
Size: 35 1630 35 4649
Fragments arranged by size:
4649 1630 35 35
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DraIII CAC_nnn'GTG
Cuts at: 2467 2467
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2492 2610 2635
Size: 54 1425 50 275 18 662 118 25
Cuts at: 2635 2859 2892 3537 3796 4685 6314 8
Size: 224 33 645 259 889 1629 43
Fragments arranged by size:
1629 1425 889 662 645 275 259 224
118 54 50 43 33 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2635 2892 3796 1537
Size: 275 823 257 904 4090
Fragments arranged by size:
4090 904 823 275 257
EarI CTCTTCn'nnn_
Cuts at: 1353 4279 1353
Size: 2926 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3359 3458 3629 4412 5009 5796 6064
Size: 393 1727 99 171 783 597 787 268
Cuts at: 6064 1239
Size: 1524
Fragments arranged by size:
1727 1524 787 783 597 393 268 171
99
EcoO109I rG'GnC_Cy
Cuts at: 2449 2927 3100 3101 2449
Size: 478 173 1 5697
Fragments arranged by size:
5697 478 173 1
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3613 3613
Size: 6349
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 3287 3380 5496 1678
Size: 1609 93 2116 2531
Fragments arranged by size:
2531 2116 1609 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4990 5022 5064 5096 1373
Size: 32 135 32 3418 32 42 32 2626
Fragments arranged by size:
3418 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5463 5647 1305
Size: 4158 184 2007
Fragments arranged by size:
4158 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4527
Fragments arranged by size:
4527 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 3332 4307 4666 4825 518
Size: 648 1041 1125 975 359 159 2042
Fragments arranged by size:
2042 1125 1041 975 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2226 2258 2273 2349 2361 2418 2490 2634
Size: 620 32 15 76 12 57 72 144
Cuts at: 2634 2746 2971 3021 3063 3142 3505 3765 4442
Size: 112 225 50 42 79 363 260 677
Cuts at: 4442 5590 5835 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 620 363 260 245 225
144 112 79 76 72 57 50 42
32 15 12
NaeI GCC'GGC
Cuts at: 2779 2859 3358 3553 2779
Size: 80 499 195 5575
Fragments arranged by size:
5575 499 195 80
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5797
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NgoMIV G'CCGG_C
Cuts at: 2777 2857 3356 3551 2777
Size: 80 499 195 5575
Fragments arranged by size:
5575 499 195 80
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 2635 3796 2635
Size: 1161 5188
NspI r_CATG'y
Cuts at: 1132 1229 3045 3707 5252 1132
Size: 97 1816 662 1545 2229
Fragments arranged by size:
2229 1816 1545 662 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2127 1108
Size: 1019 5330
PfoI T'CCnGG_A
Cuts at: 3642 3642
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 2482 2482
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 2926 3100 2926
Size: 174 6175
PspXI vC'TCGA_Gb
Cuts at: 3380 3380
Size: 6349
PstI C_TGCA'G
Cuts at: 830 3150 3701 830
Size: 2320 551 3478
Fragments arranged by size:
3478 2320 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2226 2258 2490 2746 2226
Size: 32 232 256 5829
Fragments arranged by size:
5829 256 232 32
SacII CC_GC'GG
Cuts at: 3022 3064 3022
Size: 42 6307
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 3133 3146 3697 4832 5513 5704 826
Size: 219 2088 13 551 1135 681 191 1471
Fragments arranged by size:
2088 1471 1135 681 551 219 191 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 3356 1700
Size: 1656 4693
SmaI CCC'GGG
Cuts at: 1872 2094 2508 3676 1872
Size: 222 414 1168 4545
Fragments arranged by size:
4545 1168 414 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3380 3392 3413 4453 5354 5616
Size: 197 926 1437 12 21 1040 901 262
Cuts at: 5616 820
Size: 1553
Fragments arranged by size:
1553 1437 1040 926 901 262 197 21
12
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3045 3707 1132
Size: 1913 662 3774
Fragments arranged by size:
3774 1913 662
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 2438 2918 3053 3620 1517
Size: 921 480 135 567 4246
Fragments arranged by size:
4246 921 567 480 135
StyI C'CwwG_G
Cuts at: 513 1065 3788 513
Size: 552 2723 3074
Fragments arranged by size:
3074 2723 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2119 3506 4376 4393 4546 4731 5176
Size: 1255 539 1387 870 17 153 185 445
Cuts at: 5176 325
Size: 1498
Fragments arranged by size:
1498 1387 1255 870 539 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2568 2784 4595
Size: 274 80 33 51 1443 591 216 1811
Cuts at: 4595 6307 96
Size: 1712 138
Fragments arranged by size:
1811 1712 1443 591 274 216 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 6309 6341 2931
Size: 32 3346 32 2939
Fragments arranged by size:
3346 2939 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2128 2128
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 3380 1943
Size: 1437 4912
XmaI C'CCGG_G
Cuts at: 1870 2092 2506 3674 1870
Size: 222 414 1168 4545
Fragments arranged by size:
4545 1168 414 222
XmnI GAAnn'nnTTC
Cuts at: 4478 4478
Size: 6349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2629 4157 276
Size: 53 83 186 936 1095 1528 2468
Fragments arranged by size:
2468 1528 1095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PpuMI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI