(Linear) (Six-base) MAPSORT of: KIEE2620.seq Check: 8709 from: 1 to: 1644 KIEE2620.orf With 182 enzymes: SgfI * May 6, 2016 19:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 388 793 1644 Size: 388 405 851 Fragments arranged by size: 851 405 388 AatII G_ACGT'C Cuts at: 0 626 1644 Size: 626 1018 AflIII A'CryG_T Cuts at: 0 203 1644 Size: 203 1441 AleI CACnn'nnGTG Cuts at: 0 1327 1644 Size: 1327 317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 493 1067 1644 Size: 493 574 577 Fragments arranged by size: 577 574 493 ApaI G_GGCC'C Cuts at: 0 925 1099 1644 Size: 925 174 545 Fragments arranged by size: 925 545 174 AvaI C'yCGr_G Cuts at: 0 51 87 501 1342 1375 1644 Size: 51 36 414 841 33 269 Fragments arranged by size: 841 414 269 51 36 33 BanI G'GyrC_C Cuts at: 0 1420 1644 Size: 1420 224 BanII G_rGCy'C Cuts at: 0 307 371 925 1099 1343 1644 Size: 307 64 554 174 244 301 Fragments arranged by size: 554 307 301 244 174 64 BbsI GAAGACnn'nnnn_ Cuts at: 0 773 1644 Size: 773 871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1284 1318 1644 Size: 1284 34 326 Fragments arranged by size: 1284 326 34 BclI T'GATC_A Cuts at: 0 686 699 1644 Size: 686 13 945 Fragments arranged by size: 945 686 13 BglI GCCn_nnn'nGGC Cuts at: 0 89 443 1369 1644 Size: 89 354 926 275 Fragments arranged by size: 926 354 275 89 BglII A'GATC_T Cuts at: 0 1589 1644 Size: 1589 55 BlpI GC'TnA_GC Cuts at: 0 109 481 1282 1644 Size: 109 372 801 362 Fragments arranged by size: 801 372 362 109 Bme1580I G_kGCm'C Cuts at: 0 100 295 467 925 1056 1099 1423 1644 Size: 100 195 172 458 131 43 324 221 Fragments arranged by size: 458 324 221 195 172 131 100 43 BmgBI CAC'GTC Cuts at: 0 1383 1644 Size: 1383 261 BmrI ACTGGGnnnn_n' Cuts at: 0 1467 1644 Size: 1467 177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1354 1453 1624 1644 Size: 1354 99 171 20 Fragments arranged by size: 1354 171 99 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1372 1393 1644 Size: 1372 21 251 Fragments arranged by size: 1372 251 21 BsaI GGTCTCn'nnnn_ Cuts at: 0 508 1018 1644 Size: 508 510 626 Fragments arranged by size: 626 510 508 BsaAI yAC'GTr Cuts at: 0 477 1644 Size: 477 1167 BsaHI Gr'CG_yC Cuts at: 0 131 425 623 770 1070 1544 1644 Size: 131 294 198 147 300 474 100 Fragments arranged by size: 474 300 294 198 147 131 100 BsaWI w'CCGG_w Cuts at: 0 1265 1644 Size: 1265 379 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2 32 1644 Size: 2 30 1612 Fragments arranged by size: 1612 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 28 1351 1644 Size: 25 3 1323 293 Fragments arranged by size: 1323 293 25 3 BseYI C'CCAG_C Cuts at: 0 75 249 370 493 805 871 879 1109 Size: 75 174 121 123 312 66 8 230 Cuts at: 1109 1121 1644 Size: 12 523 Fragments arranged by size: 523 312 230 174 123 121 75 66 12 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 433 610 771 900 928 1111 1644 Size: 199 234 177 161 129 28 183 533 Fragments arranged by size: 533 234 199 183 177 161 129 28 BsiEI CG_ry'CG Cuts at: 0 354 633 890 1644 Size: 354 279 257 754 Fragments arranged by size: 754 354 279 257 BsiHKAI G_wGCw'C Cuts at: 0 142 482 749 1388 1644 Size: 142 340 267 639 256 Fragments arranged by size: 639 340 267 256 142 Bsp1286I G_dGCh'C Cuts at: 0 100 142 295 307 371 467 482 749 Size: 100 42 153 12 64 96 15 267 Cuts at: 749 925 1056 1099 1343 1388 1423 1644 Size: 176 131 43 244 45 35 221 Fragments arranged by size: 267 244 221 176 153 131 100 96 64 45 43 42 35 15 12 BspEI T'CCGG_A Cuts at: 0 1265 1644 Size: 1265 379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 388 582 793 1644 Size: 388 194 211 851 Fragments arranged by size: 851 388 211 194 BsrFI r'CCGG_y Cuts at: 0 772 852 1351 1546 1644 Size: 772 80 499 195 98 Fragments arranged by size: 772 499 195 98 80 BsrGI T'GTAC_A Cuts at: 0 563 779 1644 Size: 563 216 865 Fragments arranged by size: 865 563 216 BssHII G'CGCG_C Cuts at: 0 793 1644 Size: 793 851 BstAPI GCAn_nnn'nTGC Cuts at: 0 744 1644 Size: 744 900 BstEII G'GTnAC_C Cuts at: 0 723 1644 Size: 723 921 BstXI CCAn_nnnn'nTGG Cuts at: 0 1431 1644 Size: 1431 213 BstYI r'GATC_y Cuts at: 0 1589 1644 Size: 1589 55 BtgI C'CryG_G Cuts at: 0 993 1014 1056 1644 Size: 993 21 42 588 Fragments arranged by size: 993 588 42 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1344 1538 1644 Size: 1344 194 106 Fragments arranged by size: 1344 194 106 BtsI GCAGTG_nn' Cuts at: 0 518 615 1153 1644 Size: 518 97 538 491 Fragments arranged by size: 538 518 491 97 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 189 224 1644 Size: 189 35 1420 Fragments arranged by size: 1420 189 35 DraIII CAC_nnn'GTG Cuts at: 0 462 1644 Size: 462 1182 EaeI y'GGCC_r Cuts at: 0 487 605 630 854 887 1532 1644 Size: 487 118 25 224 33 645 112 Fragments arranged by size: 645 487 224 118 112 33 25 EagI C'GGCC_G Cuts at: 0 630 887 1644 Size: 630 257 757 Fragments arranged by size: 757 630 257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1354 1453 1624 1644 Size: 1354 99 171 20 Fragments arranged by size: 1354 171 99 20 EcoO109I rG'GnC_Cy Cuts at: 0 444 922 1095 1096 1644 Size: 444 478 173 1 548 Fragments arranged by size: 548 478 444 173 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1608 1644 Size: 1608 36 HaeII r_GCGC'y Cuts at: 0 1282 1375 1644 Size: 1282 93 269 Fragments arranged by size: 1282 269 93 MslI CAynn'nnrTG Cuts at: 0 202 1327 1644 Size: 202 1125 317 Fragments arranged by size: 1125 317 202 MspA1I CmG'CkG Cuts at: 0 221 253 268 344 356 413 485 629 Size: 221 32 15 76 12 57 72 144 Cuts at: 629 741 966 1016 1058 1137 1500 1644 Size: 112 225 50 42 79 363 144 Fragments arranged by size: 363 225 221 144 144 112 79 76 72 57 50 42 32 15 12 NaeI GCC'GGC Cuts at: 0 774 854 1353 1548 1644 Size: 774 80 499 195 96 Fragments arranged by size: 774 499 195 96 80 NgoMIV G'CCGG_C Cuts at: 0 772 852 1351 1546 1644 Size: 772 80 499 195 98 Fragments arranged by size: 772 499 195 98 80 NotI GC'GGCC_GC Cuts at: 0 630 1644 Size: 630 1014 NspI r_CATG'y Cuts at: 0 1040 1644 Size: 1040 604 PflMI CCAn_nnn'nTGG Cuts at: 0 122 1644 Size: 122 1522 PfoI T'CCnGG_A Cuts at: 0 1637 1644 Size: 1637 7 PmlI CAC'GTG Cuts at: 0 477 1644 Size: 477 1167 PspOMI G'GGCC_C Cuts at: 0 921 1095 1644 Size: 921 174 549 Fragments arranged by size: 921 549 174 PspXI vC'TCGA_Gb Cuts at: 0 1375 1644 Size: 1375 269 PstI C_TGCA'G Cuts at: 0 1145 1644 Size: 1145 499 PvuII CAG'CTG Cuts at: 0 221 253 485 741 1644 Size: 221 32 232 256 903 Fragments arranged by size: 903 256 232 221 32 SacII CC_GC'GG Cuts at: 0 1017 1059 1644 Size: 1017 42 585 Fragments arranged by size: 1017 585 42 SfcI C'TryA_G Cuts at: 0 1128 1141 1644 Size: 1128 13 503 Fragments arranged by size: 1128 503 13 SgrAI Cr'CCGG_yG Cuts at: 0 1351 1644 Size: 1351 293 SmaI CCC'GGG Cuts at: 0 89 503 1644 Size: 89 414 1141 Fragments arranged by size: 1141 414 89 SmlI C'TyrA_G Cuts at: 0 1375 1387 1408 1644 Size: 1375 12 21 236 Fragments arranged by size: 1375 236 21 12 SphI G_CATG'C Cuts at: 0 1040 1644 Size: 1040 604 StuI AGG'CCT Cuts at: 0 433 913 1048 1615 1644 Size: 433 480 135 567 29 Fragments arranged by size: 567 480 433 135 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 114 1501 1644 Size: 114 1387 143 Fragments arranged by size: 1387 143 114 TatI w'GTAC_w Cuts at: 0 563 779 1644 Size: 563 216 865 Fragments arranged by size: 865 563 216 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 926 958 1644 Size: 926 32 686 Fragments arranged by size: 926 686 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 123 1644 Size: 123 1521 XhoI C'TCGA_G Cuts at: 0 1375 1644 Size: 1375 269 XmaI C'CCGG_G Cuts at: 0 87 501 1644 Size: 87 414 1143 Fragments arranged by size: 1143 414 87 ZraI GAC'GTC Cuts at: 0 624 1644 Size: 624 1020 Enzymes that do cut: AarI AatII AflIII AleI AlfI ApaI AvaI BanI BanII BbsI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EagI Eco57MI EcoO109I FalI HaeII MslI MspA1I NaeI NgoMIV NotI NspI PflMI PfoI PmlI PspOMI PspXI PstI PvuII SacII SfcI SgrAI SmaI SmlI SphI StuI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BmtI BplI Bpu10I BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BssSI BstBI BstZ17I Bsu36I ClaI DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NruI NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SrfI SspI StyI SwaI Tth111I XbaI XmnI