Restriction map for
pFN21AE2786
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BalI
BcgI BfrI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRV EcoT22I FseI FspI
HindIII HpaI KpnI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1705 GTAGAC GT^[AC][GT]AC
AccIII 2679 TCCGGA T^CCGGA
AcsI 744 GAATTC [GA]^AATT[CT]
AgeI 938 ACCGGT A^CCGGT
AlwNI 2382 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2088 CCTCAGG CC^T[ACGT]AGG
ApoI 744 GAATTC [GA]^AATT[CT]
AspI 734 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 417 CCTAGG C^CTAGG
BamHI 611 GGATCC G^GATCC
BbsI 517 GAAGAC GAAGAC(2/6)
BciVI 2583 GGATAC GTATCC(6/5)
BclI 495 TGATCA T^GATCA
BglII 2773 AGATCT A^GATCT
BlnI 417 CCTAGG C^CTAGG
BlpI 273 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 273 GCTCAGC GC^T[ACGT]AGC
BpuAI 517 GAAGAC GAAGAC(2/6)
BsaBI 573 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2679 TCCGGA T^CCGGA
BsmBI 169 GAGACG CGTCTC(1/5)
BspEI 2679 TCCGGA T^CCGGA
BssSI 2113 CACGAG CACGAG(-5/-1)
BstAPI 2381 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1083 GGTGACC G^GT[ACGT]ACC
BstPI 1083 GGTGACC G^GT[ACGT]ACC
BstXI 881 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2088 CCTCAGG CC^T[ACGT]AGG
CelII 273 GCTCAGC GC^T[ACGT]AGC
Eco81I 2088 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1083 GGTGACC G^GT[ACGT]ACC
EcoRI 744 GAATTC G^AATTC
FbaI 495 TGATCA T^GATCA
HincII 583 GTCAAC GT[CT]^[GA]AC
HindII 583 GTCAAC GT[CT]^[GA]AC
MamI 573 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2679 TCCGGA T^CCGGA
MseI 566 TTAA T^TAA
NcoI 1193 CCATGG C^CATGG
NotI 136 GCGGCCGC GC^GGCCGC
PflFI 734 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1275 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 938 ACCGGT A^CCGGT
SacI 1894 GAGCTC GAGCT^C
SapI 1521 GCTCTTC GCTCTTC(1/4)
SfiI 238 GGCCTCACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 566 TTAA T^TAA
Tth111I 734 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1275 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 774 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 20 GGCGCC G[GA]^CG[CT]C
818 GGCGCC
BanI 20 GGCGCC G^G[CT][GA]CC
818 GGCGCC
BbeI 20 GGCGCC GGCGC^C
818 GGCGCC
BbrPI 1064 CACGTG CAC^GTG
1540 CACGTG
BbvCI 1428 CCTCAGC CCTCAGC(-5/-2)
1767 CCTCAGC
BsaAI 1064 CACGTG [CT]AC^GT[GA]
1540 CACGTG
BsaHI 20 GGCGCC G[GA]^CG[CT]C
818 GGCGCC
BsiEI 137 CGGCCG CG[GA][CT]^CG
1107 CGGCCG
BstYI 611 GGATCC [GA]^GATC[CT]
2773 AGATCT
EagI 137 CGGCCG C^GGCCG
1107 CGGCCG
EclXI 137 CGGCCG C^GGCCG
1107 CGGCCG
Eco52I 137 CGGCCG C^GGCCG
1107 CGGCCG
HgaI 734 GACGC GACGC(5/10)
2571 GACGC
Hin1I 20 GGCGCC G[GA]^CG[CT]C
818 GGCGCC
KasI 20 GGCGCC G^GCGCC
818 GGCGCC
MflI 611 GGATCC [GA]^GATC[CT]
2773 AGATCT
MslI 1530 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1909 CACAGTCATG
NarI 20 GGCGCC GG^CGCC
818 GGCGCC
PaeR7I 602 CTCGAG C^TCGAG
2059 CTCGAG
PmaCI 1064 CACGTG CAC^GTG
1540 CACGTG
PmlI 1064 CACGTG CAC^GTG
1540 CACGTG
SfoI 20 GGCGCC GGC^GCC
818 GGCGCC
Tsp509I 389 AATT ^AATT
745 AATT
XhoI 602 CTCGAG C^TCGAG
2059 CTCGAG
XhoII 611 GGATCC [GA]^GATC[CT]
2773 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI ApaI ApaLI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XmaCI XmaI