Restriction map for
pFN21AE2786
(Linear) (Six-base) MAPSORT of: KIEE2786.seq Check: 9187 from: 1 to: 2808
KIEE2786.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 994 1987 2346 2808
Size: 994 993 359 462
Fragments arranged by size:
994 993 462 359
AccI GT'mk_AC
Cuts at: 0 1706 2808
Size: 1706 1102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 745 1569 2808
Size: 536 209 824 1239
Fragments arranged by size:
1239 824 536 209
AgeI A'CCGG_T
Cuts at: 0 938 2808
Size: 938 1870
AleI CACnn'nnGTG
Cuts at: 0 1534 2808
Size: 1534 1274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 106 2808
Size: 106 2702
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 160 192 2808
Size: 160 32 2616
Fragments arranged by size:
2616 160 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2387 2808
Size: 2387 421
ApaI G_GGCC'C
Cuts at: 0 149 843 1483 1948 2033 2210 2466 2658
Size: 149 694 640 465 85 177 256 192
Cuts at: 2658 2808
Size: 150
Fragments arranged by size:
694 640 465 256 192 177 150 149
85
ApaLI G'TGCA_C
Cuts at: 0 1114 1537 1906 2808
Size: 1114 423 369 902
Fragments arranged by size:
1114 902 423 369
ApoI r'AATT_y
Cuts at: 0 744 2808
Size: 744 2064
AvaI C'yCGr_G
Cuts at: 0 12 121 602 1475 1836 1845 1888 1959
Size: 12 109 481 873 361 9 43 71
Cuts at: 1959 2059 2458 2465 2618 2799 2808
Size: 100 399 7 153 181 9
Fragments arranged by size:
873 481 399 361 181 153 109 100
71 43 12 9 9 7
AvrII C'CTAG_G
Cuts at: 0 417 2808
Size: 417 2391
BamHI G'GATC_C
Cuts at: 0 611 2808
Size: 611 2197
BanI G'GyrC_C
Cuts at: 0 20 818 2808
Size: 20 798 1990
Fragments arranged by size:
1990 798 20
BanII G_rGCy'C
Cuts at: 0 149 208 514 843 1483 1810 1837 1879
Size: 149 59 306 329 640 327 27 42
Cuts at: 1879 1898 1948 2033 2060 2147 2210 2227 2249
Size: 19 50 85 27 87 63 17 22
Cuts at: 2249 2466 2637 2658 2808
Size: 217 171 21 150
Fragments arranged by size:
640 329 327 306 217 171 150 149
87 85 63 59 50 42 27 27
22 21 19 17
BbeI G_GCGC'C
Cuts at: 0 24 822 2808
Size: 24 798 1986
Fragments arranged by size:
1986 798 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 524 2808
Size: 524 2284
BbvCI CC'TCA_GC
Cuts at: 0 1429 1768 2808
Size: 1429 339 1040
Fragments arranged by size:
1429 1040 339
BciVI GTATCCnnnnn_n'
Cuts at: 0 2577 2808
Size: 2577 231
BclI T'GATC_A
Cuts at: 0 495 2808
Size: 495 2313
BglI GCCn_nnn'nGGC
Cuts at: 0 71 212 245 1825 2758 2808
Size: 71 141 33 1580 933 50
Fragments arranged by size:
1580 933 141 71 50 33
BglII A'GATC_T
Cuts at: 0 2773 2808
Size: 2773 35
BlpI GC'TnA_GC
Cuts at: 0 274 2808
Size: 274 2534
Bme1580I G_kGCm'C
Cuts at: 0 149 843 1118 1483 1541 1910 1948 2033
Size: 149 694 275 365 58 369 38 85
Cuts at: 2033 2210 2466 2658 2721 2808
Size: 177 256 192 63 87
Fragments arranged by size:
694 369 365 275 256 192 177 149
87 85 63 58 38
BmgBI CAC'GTC
Cuts at: 0 869 1577 2808
Size: 869 708 1231
Fragments arranged by size:
1231 869 708
BmrI ACTGGGnnnn_n'
Cuts at: 0 319 1383 2244 2808
Size: 319 1064 861 564
Fragments arranged by size:
1064 861 564 319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2421 2808
Size: 2421 387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1324 1582 1674 2008 2038 2707 2808
Size: 910 414 258 92 334 30 669 101
Fragments arranged by size:
910 669 414 334 258 101 92 30
Bpu10I CC'TnA_GC
Cuts at: 0 1429 1768 2473 2808
Size: 1429 339 705 335
Fragments arranged by size:
1429 705 339 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 2808
Size: 511 2297
BsaI GGTCTCn'nnnn_
Cuts at: 0 690 1042 1390 2808
Size: 690 352 348 1418
Fragments arranged by size:
1418 690 352 348
BsaAI yAC'GTr
Cuts at: 0 1066 1542 2808
Size: 1066 476 1266
Fragments arranged by size:
1266 1066 476
BsaBI GATnn'nnATC
Cuts at: 0 577 2808
Size: 577 2231
BsaHI Gr'CG_yC
Cuts at: 0 21 819 2808
Size: 21 798 1989
Fragments arranged by size:
1989 798 21
BsaWI w'CCGG_w
Cuts at: 0 77 938 2679 2808
Size: 77 861 1741 129
Fragments arranged by size:
1741 861 129 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 1438 1468 1672 1702 1797 1827
Size: 160 30 1248 30 204 30 95 30
Cuts at: 1827 2236 2266 2808
Size: 409 30 542
Fragments arranged by size:
1248 542 409 204 160 95 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 617 925 1906 2001 2641 2698 2808
Size: 617 308 981 95 640 57 110
Fragments arranged by size:
981 640 617 308 110 95 57
BseYI C'CCAG_C
Cuts at: 0 356 1134 1323 1921 2147 2157 2808
Size: 356 778 189 598 226 10 651
Fragments arranged by size:
778 651 598 356 226 189 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 316 575 787 1635 1695 2437 2808
Size: 102 214 259 212 848 60 742 371
Fragments arranged by size:
848 742 371 259 214 212 102 60
BsiEI CG_ry'CG
Cuts at: 0 140 1110 2808
Size: 140 970 1698
Fragments arranged by size:
1698 970 140
BsiHKAI G_wGCw'C
Cuts at: 0 1118 1310 1541 1898 1910 2024 2808
Size: 1118 192 231 357 12 114 784
Fragments arranged by size:
1118 784 357 231 192 114 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 163 2808
Size: 163 2645
Bsp1286I G_dGCh'C
Cuts at: 0 149 208 514 843 1118 1310 1483 1541
Size: 149 59 306 329 275 192 173 58
Cuts at: 1541 1810 1837 1879 1898 1910 1948 2024 2033
Size: 269 27 42 19 12 38 76 9
Cuts at: 2033 2060 2147 2210 2227 2249 2466 2637 2658
Size: 27 87 63 17 22 217 171 21
Cuts at: 2658 2721 2808
Size: 63 87
Fragments arranged by size:
329 306 275 269 217 192 173 171
149 87 87 76 63 63 59 58
42 38 27 27 22 21 19 17
12 9
BspEI T'CCGG_A
Cuts at: 0 2679 2808
Size: 2679 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 994 1866 1987 2346 2808
Size: 994 872 121 359 462
Fragments arranged by size:
994 872 462 359 121
BsrBI CCG'CTC
Cuts at: 0 525 1288 1505 1568 1607 2631 2808
Size: 525 763 217 63 39 1024 177
Fragments arranged by size:
1024 763 525 217 177 63 39
BsrFI r'CCGG_y
Cuts at: 0 235 534 938 1092 1199 2808
Size: 235 299 404 154 107 1609
Fragments arranged by size:
1609 404 299 235 154 107
BssSI C'ACGA_G
Cuts at: 0 2113 2808
Size: 2113 695
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2387 2808
Size: 2387 421
BstEII G'GTnAC_C
Cuts at: 0 1083 2808
Size: 1083 1725
BstXI CCAn_nnnn'nTGG
Cuts at: 0 888 2808
Size: 888 1920
BstYI r'GATC_y
Cuts at: 0 611 2773 2808
Size: 611 2162 35
Fragments arranged by size:
2162 611 35
Bsu36I CC'TnA_GG
Cuts at: 0 2089 2808
Size: 2089 719
BtgI C'CryG_G
Cuts at: 0 32 69 140 1167 1193 1532 2364 2590
Size: 32 37 71 1027 26 339 832 226
Cuts at: 2590 2808
Size: 218
Fragments arranged by size:
1027 832 339 226 218 71 37 32
26
BtsI GCAGTG_nn'
Cuts at: 0 1515 1623 2747 2808
Size: 1515 108 1124 61
Fragments arranged by size:
1515 1124 108 61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1335 1370 2808
Size: 1335 35 1438
Fragments arranged by size:
1438 1335 35
EaeI y'GGCC_r
Cuts at: 0 137 1107 1283 1356 1599 1628 1671 1939
Size: 137 970 176 73 243 29 43 268
Cuts at: 1939 2808
Size: 869
Fragments arranged by size:
970 869 268 243 176 137 73 43
29
EagI C'GGCC_G
Cuts at: 0 137 1107 2808
Size: 137 970 1701
Fragments arranged by size:
1701 970 137
EarI CTCTTCn'nnn_
Cuts at: 0 329 1528 2342 2618 2808
Size: 329 1199 814 276 190
Fragments arranged by size:
1199 814 329 276 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2387 2808
Size: 2387 421
EcoICRI GAG'CTC
Cuts at: 0 1896 2808
Size: 1896 912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 745 910 1324 1569 1582 1674 2008
Size: 536 209 165 414 245 13 92 334
Cuts at: 2008 2038 2707 2808
Size: 30 669 101
Fragments arranged by size:
669 536 414 334 245 209 165 101
92 30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 421 1338 2218 2808
Size: 421 917 880 590
Fragments arranged by size:
917 880 590 421
EcoO109I rG'GnC_Cy
Cuts at: 0 145 839 840 1425 1479 1855 1945 2029
Size: 145 694 1 585 54 376 90 84
Cuts at: 2029 2096 2206 2207 2462 2469 2543 2604 2654
Size: 67 110 1 255 7 74 61 50
Cuts at: 2654 2655 2808
Size: 1 153
Fragments arranged by size:
694 585 376 255 153 145 110 90
84 74 67 61 54 50 7 1
1 1
EcoRI G'AATT_C
Cuts at: 0 744 2808
Size: 744 2064
HaeII r_GCGC'y
Cuts at: 0 24 281 822 2174 2808
Size: 24 257 541 1352 634
Fragments arranged by size:
1352 634 541 257 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 596 628 1438 1470 1797 1829 1957 1989
Size: 596 32 810 32 327 32 128 32
Cuts at: 1989 2107 2139 2236 2268 2808
Size: 118 32 97 32 540
Fragments arranged by size:
810 596 540 327 128 118 97 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 585 2808
Size: 585 2223
KasI G'GCGC_C
Cuts at: 0 20 818 2808
Size: 20 798 1990
Fragments arranged by size:
1990 798 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 2808
Size: 896 1912
MslI CAynn'nnrTG
Cuts at: 0 1534 1913 2808
Size: 1534 379 895
Fragments arranged by size:
1534 895 379
MspA1I CmG'CkG
Cuts at: 0 71 108 114 142 444 501 1295 1589
Size: 71 37 6 28 302 57 794 294
Cuts at: 1589 2182 2366 2384 2808
Size: 593 184 18 424
Fragments arranged by size:
794 593 424 302 294 184 71 57
37 28 18 6
NarI GG'CG_CC
Cuts at: 0 21 819 2808
Size: 21 798 1989
Fragments arranged by size:
1989 798 21
NcoI C'CATG_G
Cuts at: 0 1193 2808
Size: 1193 1615
NotI GC'GGCC_GC
Cuts at: 0 137 2808
Size: 137 2671
PasI CC'CwG_GG
Cuts at: 0 25 2265 2808
Size: 25 2240 543
Fragments arranged by size:
2240 543 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 1281 2808
Size: 1281 1527
PfoI T'CCnGG_A
Cuts at: 0 2723 2808
Size: 2723 85
PmlI CAC'GTG
Cuts at: 0 1066 1542 2808
Size: 1066 476 1266
Fragments arranged by size:
1266 1066 476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 496 528 2808
Size: 160 32 304 32 2280
Fragments arranged by size:
2280 304 160 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1855 2096 2469 2543 2808
Size: 1855 241 373 74 265
Fragments arranged by size:
1855 373 265 241 74
PspOMI G'GGCC_C
Cuts at: 0 145 839 1479 1944 2029 2206 2462 2654
Size: 145 694 640 465 85 177 256 192
Cuts at: 2654 2808
Size: 154
Fragments arranged by size:
694 640 465 256 192 177 154 145
85
PspXI vC'TCGA_Gb
Cuts at: 0 602 2059 2808
Size: 602 1457 749
Fragments arranged by size:
1457 749 602
PstI C_TGCA'G
Cuts at: 0 459 977 1984 2766 2808
Size: 459 518 1007 782 42
Fragments arranged by size:
1007 782 518 459 42
PvuII CAG'CTG
Cuts at: 0 501 1589 2182 2808
Size: 501 1088 593 626
Fragments arranged by size:
1088 626 593 501
SacI G_AGCT'C
Cuts at: 0 1898 2808
Size: 1898 910
SacII CC_GC'GG
Cuts at: 0 72 143 2367 2808
Size: 72 71 2224 441
Fragments arranged by size:
2224 441 72 71
SanDI GG'GwC_CC
Cuts at: 0 2543 2808
Size: 2543 265
SapI GCTCTTCn'nnn_
Cuts at: 0 1528 2808
Size: 1528 1280
SfcI C'TryA_G
Cuts at: 0 455 973 1980 1998 2762 2808
Size: 455 518 1007 18 764 46
Fragments arranged by size:
1007 764 518 455 46 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 245 2808
Size: 245 2563
SfoI GGC'GCC
Cuts at: 0 22 820 2808
Size: 22 798 1988
Fragments arranged by size:
1988 798 22
SmaI CCC'GGG
Cuts at: 0 1477 2460 2467 2620 2801 2808
Size: 1477 983 7 153 181 7
Fragments arranged by size:
1477 983 181 153 7 7
SmlI C'TyrA_G
Cuts at: 0 526 602 2059 2808
Size: 526 76 1457 749
Fragments arranged by size:
1457 749 526 76
StyI C'CwwG_G
Cuts at: 0 164 417 689 1193 1370 1936 2538 2808
Size: 164 253 272 504 177 566 602 270
Fragments arranged by size:
602 566 504 272 270 253 177 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 320 2784 2808
Size: 320 2464 24
Fragments arranged by size:
2464 320 24
TatI w'GTAC_w
Cuts at: 0 438 896 931 2420 2808
Size: 438 458 35 1489 388
Fragments arranged by size:
1489 458 438 388 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1672 1704 2808
Size: 1672 32 1104
Fragments arranged by size:
1672 1104 32
Tth111I GACn'n_nGTC
Cuts at: 0 737 2808
Size: 737 2071
XbaI T'CTAG_A
Cuts at: 0 774 2808
Size: 774 2034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 756 1698 1983 2808
Size: 756 942 285 825
Fragments arranged by size:
942 825 756 285
XhoI C'TCGA_G
Cuts at: 0 602 2059 2808
Size: 602 1457 749
Fragments arranged by size:
1457 749 602
XmaI C'CCGG_G
Cuts at: 0 1475 2458 2465 2618 2799 2808
Size: 1475 983 7 153 181 9
Fragments arranged by size:
1475 983 181 153 9 7
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI MslI
MspA1I NarI NcoI NotI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII
SanDI SapI SfcI SfiI SfoI SmaI SmlI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AscI
AseI AsiSI BaeI BcgI BfrBI BmtI BsiWI BsmI
BspHI BsrDI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
DraI DraIII DrdI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI
NgoMIV NheI NruI NsiI NspI PacI PciI PmeI
PshAI PsiI PsrI PvuI RsrII SalI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI XmnI ZraI