################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AhdI Alw44I AlwI AocI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoRII EcoRV EcoT14I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI MvaI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 431 CCGC CCGC(-3/-1) AcsI 51 GAATTC [GA]^AATT[CT] AgeI 410 ACCGGT A^CCGGT Aor51HI 621 AGCGCT AGC^GCT ApaI 634 GGGCCC GGGCC^C ApoI 51 GAATTC [GA]^AATT[CT] BbsI 70 GTCTTC GAAGAC(2/6) BbvCI 445 GCTGAGG CCTCAGC(-5/-2) BcnI 120 CCCGG CC^[GC]GG BfaI 619 CTAG C^TAG BpmI 393 CTCCAG CTGGAG(16/14) BpuAI 70 GTCTTC GAAGAC(2/6) BsaWI 410 ACCGGT [AT]^CCGG[AT] BseRI 313 GAGGAG GAGGAG(10/8) BsgI 595 CTGCAC GTGCAG(16/14) BspMI 88 ACCTGC ACCTGC(4/8) BsrFI 410 ACCGGT [GA]^CCGG[CT] BssKI 120 CCCGG ^CC[ACGT]GG BstEII 123 GGTCACC G^GT[ACGT]ACC BstF5I 183 CATCC GGATG(2/0) BstPI 123 GGTCACC G^GT[ACGT]ACC CfoI 622 GCGC GCG^C Cfr10I 410 ACCGGT [GA]^CCGG[CT] Eco47III 621 AGCGCT AGC^GCT EcoO65I 123 GGTCACC G^GT[ACGT]ACC EcoRI 51 GAATTC G^AATTC EcoT22I 210 ATGCAT ATGCA^T FauI 430 CCCGC CCCGC(4/6) FokI 183 CATCC GGATG(9/13) HaeII 621 AGCGCT [GA]GCGC^[CT] HhaI 622 GCGC GCG^C HinP1I 622 GCGC G^CGC HindIII 497 AAGCTT A^AGCTT MaeI 619 CTAG C^TAG MaeIII 124 GTCAC ^GT[ACGT]AC MseI 469 TTAA T^TAA MslI 96 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 431 CCGCTG C[AC]G^C[GT]G NciI 120 CCCGG CC^[GC]GG NsiI 210 ATGCAT ATGCA^T NspI 599 ACATGC [GA]CATG^[CT] PflMI 287 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 410 ACCGGT A^CCGGT PleI 583 GAGTC GAGTC(4/5) SacI 306 GAGCTC GAGCT^C ScrFI 120 CCCGG CC^[ACGT]GG Tru9I 469 TTAA T^TAA Tsp45I 124 GTCAC ^GT[GC]AC Van91I 287 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 105 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 348 GAAGATGTTC AspI 118 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 422 GACACTGTC BanII 306 GAGCTC G[GA]GC[CT]^C 634 GGGCCC BsmFI 653 GGGAC GGGAC(10/14) 428 GTCCC BsmI 208 GAATGC GAATGC(1/-1) 329 GCATTC Cac8I 152 GCAGGC GC[ACGT]^[ACGT]GC 374 GCCTGC DraII 154 AGGCCCT [GA]G^G[ACGT]CC[CT] 633 AGGGCCC EcoO109I 154 AGGCCCT [GA]G^G[ACGT]CC[CT] 633 AGGGCCC HaeIII 155 GGCC GG^CC 635 GGCC HapII 121 CCGG C^CGG 411 CCGG HpaII 121 CCGG C^CGG 411 CCGG HphI 86 TCACC GGTGA(8/7) 125 TCACC MspI 121 CCGG C^CGG 411 CCGG PflFI 118 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 422 GACACTGTC TaqI 6 TCGA T^CGA 252 TCGA Tth111I 118 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 422 GACACTGTC TthHB8I 6 TCGA T^CGA 252 TCGA XmnI 105 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 348 GAAGATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI AlwNI AspHI BbvI BmyI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI Cfr13I DdeI Fnu4HI HinfI ItaI MboII MnlI MwoI NlaIII NlaIV Sau96I TfiI TseI Tsp509I TspRI