################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AlwI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BcgI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 1285 GTCTAC GT^[AC][GT]AC AccII 476 CGCG CG^CG AcsI 820 AAATTC [GA]^AATT[CT] AflIII 722 ACACGT A^C[GA][CT]GT Alw44I 745 GTGCAC G^TGCAC AlwNI 620 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 745 GTGCAC G^TGCAC ApoI 820 AAATTC [GA]^AATT[CT] Asp700I 423 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 343 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 140 TGGCCA TGG^CCA BbvCI 617 CCTCAGC CCTCAGC(-5/-2) BciVI 1082 GTATCC GTATCC(6/5) BclI 1112 TGATCA T^GATCA BfaI 289 CTAG C^TAG BglI 636 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 809 AGATCT A^GATCT BlpI 585 GCTCAGC GC^T[ACGT]AGC Bpu1102I 585 GCTCAGC GC^T[ACGT]AGC BsaAI 723 CACGTA [CT]AC^GT[GA] BseRI 663 CTCCTC GAGGAG(10/8) BsgI 706 GTGCAG GTGCAG(16/14) BsrFI 161 GCCGGC [GA]^CCGG[CT] BssSI 1200 CACGAG CACGAG(-5/-1) BstBI 1246 TTCGAA TT^CGAA BstUI 476 CGCG CG^CG BstXI 332 CCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 809 AGATCT [GA]^GATC[CT] CelII 585 GCTCAGC GC^T[ACGT]AGC Cfr10I 161 GCCGGC [GA]^CCGG[CT] FbaI 1112 TGATCA T^GATCA HaeII 1067 GGCGCT [GA]GCGC^[CT] HphI 1181 TCACC GGTGA(8/7) MaeI 289 CTAG C^TAG MaeII 724 ACGT A^CGT MaeIII 1198 GTCAC ^GT[ACGT]AC MflI 809 AGATCT [GA]^GATC[CT] MluNI 140 TGGCCA TGG^CCA MscI 140 TGGCCA TGG^CCA MseI 230 TTAA T^TAA MvnI 476 CGCG CG^CG NaeI 161 GCCGGC GCC^GGC NgoMIV 161 GCCGGC G^CCGGC NspV 1246 TTCGAA TT^CGAA PflFI 343 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 8 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 850 GAGCTC GAGCT^C SbfI 1188 CCTGCAGG CCTGCA^GG SfuI 1246 TTCGAA TT^CGAA Sse8387I 1188 CCTGCAGG CCTGCA^GG Tru9I 230 TTAA T^TAA Tsp45I 1198 GTCAC ^GT[GC]AC Tsp509I 821 AATT ^AATT Tth111I 343 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 8 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1184 CCATCCTGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 809 AGATCT [GA]^GATC[CT] XmnI 423 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C 378 GGCGTC BsaHI 3 GGCGTC G[GA]^CG[CT]C 378 GGCGTC BsiEI 159 CGGCCG CG[GA][CT]^CG 201 CGGCCG BsmBI 402 CGTCTC CGTCTC(1/5) 990 CGTCTC BsmFI 504 GGGAC GGGAC(10/14) 1208 GGGAC BspMI 1139 ACCTGC ACCTGC(4/8) 1191 GCAGGT BsrBI 610 CCGCTC CCGCTC(-3/-3) 1089 CCGCTC BsrDI 657 CATTGC GCAATG(2/0) 1233 CATTGC BsrI 410 CCAGT ACTGG(1/-1) 1259 CCAGT CfoI 804 GCGC GCG^C 1068 GCGC EagI 159 CGGCCG C^GGCCG 201 CGGCCG EclXI 159 CGGCCG C^GGCCG 201 CGGCCG Eco52I 159 CGGCCG C^GGCCG 201 CGGCCG EcoNI 66 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 339 CCTGGACCAGG EcoT14I 353 CCAAGG C^C[AT][AT]GG 998 CCAAGG HhaI 804 GCGC GCG^C 1068 GCGC Hin1I 3 GGCGTC G[GA]^CG[CT]C 378 GGCGTC HinP1I 804 GCGC G^CGC 1068 GCGC HinfI 249 GACTC G^A[ACGT]TC 1210 GACTC MslI 699 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 996 CACCAAGGTG MspA1I 12 CAGCTG C[AC]G^C[GT]G 620 CAGCTG PleI 249 GACTC GAGTC(4/5) 1210 GACTC PstI 418 CTGCAG CTGCA^G 1189 CTGCAG PvuII 12 CAGCTG CAG^CTG 620 CAGCTG SexAI 314 ACCAGGT A^CC[AT]GGT 344 ACCAGGT SfaNI 88 GATGC GCATC(5/9) 323 GCATC SfcI 418 CTGCAG C^T[GA][CT]AG 1189 CTGCAG StyI 353 CCAAGG C^C[AT][AT]GG 998 CCAAGG TaqI 116 TCGA T^CGA 1247 TCGA TspRI 411 CAGTG CA[GC]TG[ACGT][ACGT]^ 1260 CAGTG TthHB8I 116 TCGA T^CGA 1247 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI ApaI AspHI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SmaI TseI XmaCI XmaI