(Circular) (Six-base) MAPSORT of: pFN21AE3098.seq Check: 1751 from: 1 to: 6010 pFN21AE3098 6010 bp With 182 enzymes: SgfI * September 4, 2015 17:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3153 3187 3153 Size: 34 5976 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3291 3353 1937 Size: 1354 62 4594 Fragments arranged by size: 4594 1354 62 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 3158 4073 5457 5725 1632 Size: 725 801 915 1384 268 1917 Fragments arranged by size: 1917 1384 915 801 725 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 2727 4909 5886 1203 Size: 1524 2182 977 1327 Fragments arranged by size: 2182 1524 1327 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 3005 1166 Size: 1839 4171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6010 AlwNI CAG_nnn'CTG Cuts at: 2630 3753 5325 2630 Size: 1123 1572 3315 Fragments arranged by size: 3315 1572 1123 ApaI G_GGCC'C Cuts at: 2064 2874 2936 3280 2064 Size: 810 62 344 4794 Fragments arranged by size: 4794 810 344 62 ApaLI G'TGCA_C Cuts at: 2750 4067 5223 2750 Size: 1317 1156 3537 Fragments arranged by size: 3537 1317 1156 ApoI r'AATT_y Cuts at: 1493 2825 3319 3545 3697 5806 1493 Size: 1332 494 226 152 2109 1697 Fragments arranged by size: 2109 1697 1332 494 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2049 2063 2831 2935 3149 3335 Size: 73 96 10 14 768 104 214 186 Cuts at: 3335 1870 Size: 4545 Fragments arranged by size: 4545 768 214 186 104 96 73 14 10 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3331 4786 618 Size: 325 217 627 1544 1455 1842 Fragments arranged by size: 1842 1544 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2114 2607 2859 2874 2936 3280 3329 Size: 673 50 493 252 15 62 344 49 Cuts at: 3329 1391 Size: 4072 Fragments arranged by size: 4072 673 493 344 252 62 50 49 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 2458 3241 928 Size: 971 359 200 783 3697 Fragments arranged by size: 3697 971 783 359 200 BbvCI CC'TCA_GC Cuts at: 2623 2623 Size: 6010 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4201 4235 1903 Size: 34 2264 34 3678 Fragments arranged by size: 3678 2264 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3098 3902 5112 3098 Size: 804 1210 3996 Fragments arranged by size: 3996 1210 804 BclI T'GATC_A Cuts at: 1548 3117 1548 Size: 1569 4441 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2647 4621 136 Size: 107 122 71 631 605 975 1974 1525 Fragments arranged by size: 1974 1525 975 631 605 122 107 71 BglII A'GATC_T Cuts at: 2814 6005 2814 Size: 3191 2819 BlpI GC'TnA_GC Cuts at: 2591 3427 2591 Size: 836 5174 Bme1580I G_kGCm'C Cuts at: 1790 2064 2754 2874 2936 3280 4071 5227 1790 Size: 274 690 120 62 344 791 1156 2573 Fragments arranged by size: 2573 1156 791 690 344 274 120 62 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3741 4699 442 Size: 416 53 83 399 24 2324 958 1753 Fragments arranged by size: 2324 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2509 2708 2813 3083 3197 4670 1239 Size: 1270 199 105 270 114 1473 2579 Fragments arranged by size: 2579 1473 1270 270 199 114 105 Bpu10I CC'TnA_GC Cuts at: 2623 2623 Size: 6010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4135 5000 5298 4135 Size: 865 298 4847 Fragments arranged by size: 4847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2359 2712 3218 4673 882 Size: 617 860 353 506 1455 2219 Fragments arranged by size: 2219 1455 860 617 506 353 BsaAI yAC'GTr Cuts at: 493 1204 2730 3801 493 Size: 711 1526 1071 2702 Fragments arranged by size: 2702 1526 1071 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2384 3817 4199 Size: 53 83 186 936 476 375 1433 382 Cuts at: 4199 4834 275 Size: 635 1451 Fragments arranged by size: 1451 1433 936 635 476 382 375 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2339 2369 3035 Size: 30 165 30 687 30 219 30 666 Cuts at: 3035 3065 1178 Size: 30 4123 Fragments arranged by size: 4123 687 666 219 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2659 1186 Size: 1473 4537 BseYI C'CCAG_C Cuts at: 3279 5213 5709 3279 Size: 1934 496 3580 Fragments arranged by size: 3580 1934 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2732 1163 Size: 573 996 4441 Fragments arranged by size: 4441 996 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2167 2209 3460 4221 5249 1540 Size: 275 186 166 42 1251 761 1028 2301 Fragments arranged by size: 2301 1251 1028 761 275 186 166 42 BsiHKAI G_wGCw'C Cuts at: 2754 2859 3014 3273 4071 4156 5227 2754 Size: 105 155 259 798 85 1071 3537 Fragments arranged by size: 3537 1071 798 259 155 105 85 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 BsmBI CGTCTCn'nnnn_ Cuts at: 2413 3001 2413 Size: 588 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2114 2607 2754 2859 2874 2936 Size: 399 274 50 493 147 105 15 62 Cuts at: 2936 3014 3273 3280 3329 4071 4156 5227 1391 Size: 78 259 7 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 399 274 259 147 105 85 78 62 50 49 15 7 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3153 3187 3365 844 Size: 2309 34 178 3489 Fragments arranged by size: 3489 2309 178 34 BsrBI CCG'CTC Cuts at: 1394 1823 2617 3096 3897 1394 Size: 429 794 479 801 3507 Fragments arranged by size: 3507 801 794 479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2661 3237 4505 4679 65 Size: 1183 1413 576 1268 174 1396 Fragments arranged by size: 1413 1396 1268 1183 576 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2166 4654 4841 1700 Size: 110 356 2488 187 2869 Fragments arranged by size: 2869 2488 356 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 3205 4070 5082 3205 Size: 865 1012 4133 Fragments arranged by size: 4133 1012 865 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6010 BstBI TT'CG_AA Cuts at: 3252 4814 3252 Size: 1562 4448 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstXI CCAn_nnnn'nTGG Cuts at: 2344 2344 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2814 3340 3707 4092 4109 5561 5647 5659 Size: 842 526 367 385 17 1452 86 12 Cuts at: 5659 6005 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 842 526 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4519 Fragments arranged by size: 4519 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 3526 4320 4340 3526 Size: 794 20 5196 Fragments arranged by size: 5196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5017 809 Size: 507 3701 1802 Fragments arranged by size: 3701 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2164 2206 Size: 54 1425 50 275 18 315 19 42 Cuts at: 2206 2758 3457 4346 5975 8 Size: 552 699 889 1629 43 Fragments arranged by size: 1629 1425 889 699 552 315 275 54 50 43 42 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2164 2206 3457 1537 Size: 275 352 42 1251 4090 Fragments arranged by size: 4090 1251 352 275 42 EarI CTCTTCn'nnn_ Cuts at: 1353 3940 1353 Size: 2587 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2185 4597 4971 5117 387 Size: 1064 138 596 2412 374 146 1280 Fragments arranged by size: 2412 1280 1064 596 374 146 138 EcoICRI GAG'CTC Cuts at: 2857 2857 Size: 6010 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2509 2708 2813 3083 3158 3197 Size: 393 725 152 199 105 270 75 39 Cuts at: 3197 4073 4670 5457 5725 1239 Size: 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 725 597 393 270 268 199 152 105 75 39 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2348 2075 Size: 273 5737 EcoO109I rG'GnC_Cy Cuts at: 2068 2140 2870 2871 2932 3276 2068 Size: 72 730 1 61 344 4802 Fragments arranged by size: 4802 730 344 72 61 1 EcoRI G'AATT_C Cuts at: 3319 3697 3319 Size: 378 5632 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 3076 5157 1678 Size: 1398 2081 2531 Fragments arranged by size: 2531 2081 1398 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 4651 4683 4725 Size: 32 135 32 767 32 2280 32 42 Cuts at: 4725 4757 1373 Size: 32 2626 Fragments arranged by size: 2626 2280 767 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3600 669 Size: 988 281 1416 246 3079 Fragments arranged by size: 3079 1416 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 5124 5308 1305 Size: 732 3087 184 2007 Fragments arranged by size: 3087 2007 732 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 10 Size: 54 1425 343 315 3873 Fragments arranged by size: 3873 1425 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 2708 3005 3968 4327 4486 518 Size: 648 1542 297 963 359 159 2042 Fragments arranged by size: 2042 1542 963 648 359 297 159 MspA1I CmG'CkG Cuts at: 1606 2019 2627 3426 4103 5251 5496 1606 Size: 413 608 799 677 1148 245 2120 Fragments arranged by size: 2120 1148 799 677 608 413 245 NaeI GCC'GGC Cuts at: 2168 2168 Size: 6010 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4519 Fragments arranged by size: 4519 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NgoMIV G'CCGG_C Cuts at: 2166 2166 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 3368 4913 1132 Size: 97 2139 1545 2229 Fragments arranged by size: 2229 2139 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6010 PciI A'CATG_T Cuts at: 4909 4909 Size: 6010 PflMI CCAn_nnn'nTGG Cuts at: 1108 2019 1108 Size: 911 5099 PfoI T'CCnGG_A Cuts at: 2133 2685 2925 2133 Size: 552 240 5218 Fragments arranged by size: 5218 552 240 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PspOMI G'GGCC_C Cuts at: 2060 2870 2932 3276 2060 Size: 810 62 344 4794 Fragments arranged by size: 4794 810 344 62 PstI C_TGCA'G Cuts at: 830 2427 3198 3362 830 Size: 1597 771 164 3478 Fragments arranged by size: 3478 1597 771 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2019 2627 2019 Size: 608 5402 SacI G_AGCT'C Cuts at: 2859 2859 Size: 6010 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SbfI CC_TGCA'GG Cuts at: 3198 3362 3198 Size: 164 5846 ScaI AGT'ACT Cuts at: 4258 5970 4258 Size: 1712 4298 SexAI A'CCwGG_T Cuts at: 2319 2349 2319 Size: 30 5980 SfcI C'TryA_G Cuts at: 826 1045 2423 3194 3358 4493 5174 5365 826 Size: 219 1378 771 164 1135 681 191 1471 Fragments arranged by size: 1471 1378 1135 771 681 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 2041 2051 2937 3337 1872 Size: 169 10 886 400 4545 Fragments arranged by size: 4545 886 400 169 10 SmlI C'TyrA_G Cuts at: 820 1017 1943 4114 5015 5277 820 Size: 197 926 2171 901 262 1553 Fragments arranged by size: 2171 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 3003 3449 513 Size: 552 939 354 645 446 3074 Fragments arranged by size: 3074 939 645 552 446 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4037 4054 4207 4392 4837 325 Size: 1255 2457 17 153 185 445 1498 Fragments arranged by size: 2457 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2964 3111 3156 Size: 274 80 33 51 1443 987 147 45 Cuts at: 3156 4256 5968 96 Size: 1100 1712 138 Fragments arranged by size: 1712 1443 1100 987 274 147 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 5970 6002 2090 Size: 32 3848 32 2098 Fragments arranged by size: 3848 2098 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2351 1316 Size: 1035 4975 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3196 3196 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 2039 2049 2935 3335 1870 Size: 169 10 886 400 4545 Fragments arranged by size: 4545 886 400 169 10 XmnI GAAnn'nnTTC Cuts at: 2432 4139 2432 Size: 1707 4303 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI