Restriction map for
pFN21AE3896
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BspDI BspEI BspLU11I BspMI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 820 AACGTT AA^CGTT
AcsI 251 AAATTC [GA]^AATT[CT]
ApaI 437 GGGCCC GGGCC^C
ApoI 251 AAATTC [GA]^AATT[CT]
AspHI 940 GTGCTC G[AT]GC[AT]^C
AvaI 440 CCCGGG C^[CT]CG[GA]G
AvaII 660 GGACC G^G[AT]CC
BanI 404 GGTGCC G^G[CT][GA]CC
BanII 437 GGGCCC G[GA]GC[CT]^C
BpmI 99 CTCCAG CTGGAG(16/14)
BsaAI 116 CACGTA [CT]AC^GT[GA]
BsgI 418 GTGCAG GTGCAG(16/14)
BsiHKAI 940 GTGCTC G[AT]GC[AT]^C
BsmBI 623 GAGACG CGTCTC(1/5)
BsmFI 764 GGGAC GGGAC(10/14)
BsmI 111 GCATTC GAATGC(1/-1)
BsoBI 440 CCCGGG C^[CT]CG[GA]G
Bsp1407I 729 TGTACA T^GTACA
BspHI 306 TCATGA T^CATGA
BsrBI 703 GAGCGG CCGCTC(-3/-3)
BsrDI 123 CATTGC GCAATG(2/0)
BsrFI 59 ACCGGC [GA]^CCGG[CT]
BsrGI 729 TGTACA T^GTACA
CfoI 496 GCGC GCG^C
Cfr10I 59 ACCGGC [GA]^CCGG[CT]
DraI 318 TTTAAA TTT^AAA
FauI 705 GCGGG CCCGC(4/6)
HgaI 22 GACGC GACGC(5/10)
HhaI 496 GCGC GCG^C
HinP1I 496 GCGC G^CGC
HphI 95 TCACC GGTGA(8/7)
MspA1I 64 CAGCTG C[AC]G^C[GT]G
NspI 357 ACATGC [GA]CATG^[CT]
Psp1406I 820 AACGTT AA^CGTT
PstI 505 CTGCAG CTGCA^G
PvuII 64 CAGCTG CAG^CTG
RcaI 306 TCATGA T^CATGA
SbfI 504 CCTGCAGG CCTGCA^GG
SfcI 505 CTGCAG C^T[GA][CT]AG
SmaI 440 CCCGGG CCC^GGG
Sse8387I 504 CCTGCAGG CCTGCA^GG
SspBI 729 TGTACA T^GTACA
XcmI 220 CCACCTCCTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 187 GATCC GGATC(4/5)
578 GATCC
AlwNI 421 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
499 CAGGCCCTG
BalI 2 TGGCCA TGG^CCA
296 TGGCCA
BglI 415 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
487 GCCTCGCTGGC
BmyI 437 GGGCCC G[AGT]GC[ACT]^C
940 GTGCTC
BsaJI 332 CCCTGG C^C[ACGT][ACGT]GG
440 CCCGGG
BsiYI 435 CCGGGCCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
521 CCAGCTTCCGG
BslI 435 CCGGGCCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
521 CCAGCTTCCGG
BsmAI 623 GAGAC GTCTC(1/5)
969 GTCTC
Bsp1286I 437 GGGCCC G[AGT]GC[ACT]^C
940 GTGCTC
BsrI 165 ACTGG ACTGG(1/-1)
663 CCAGT
BstAPI 420 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
498 GCAGGCCCTGC
BstNI 8 CCAGG CC^[AT]GG
333 CCTGG
BstYI 186 AGATCC [GA]^GATC[CT]
577 AGATCC
DraII 422 AGGCCCT [GA]G^G[ACGT]CC[CT]
500 AGGCCCT
EcoO109I 422 AGGCCCT [GA]G^G[ACGT]CC[CT]
500 AGGCCCT
EcoRII 8 CCAGG ^CC[AT]GG
333 CCTGG
MaeIII 89 GTCAC ^GT[ACGT]AC
208 GTGAC
MflI 186 AGATCC [GA]^GATC[CT]
577 AGATCC
MluNI 2 TGGCCA TGG^CCA
296 TGGCCA
MscI 2 TGGCCA TGG^CCA
296 TGGCCA
MslI 687 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
933 CATCATTGTG
MvaI 8 CCAGG CC^[AT]GG
333 CCTGG
NlaIV 404 GGTGCC GG[ACGT]^[ACGT]CC
437 GGGCCC
SapI 700 GAAGAGC GCTCTTC(1/4)
393 GCTCTTC
SfaNI 903 GATGC GCATC(5/9)
176 GCATC
SspI 756 AATATT AAT^ATT
918 AATATT
Tsp45I 89 GTCAC ^GT[GC]AC
208 GTGAC
TspRI 164 CACTG CA[GC]TG[ACGT][ACGT]^
689 CACTG
XhoII 186 AGATCC [GA]^GATC[CT]
577 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BssKI BstF5I Cac8I Cfr13I DdeI
DpnII EaeI EarI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeII MboI MboII MnlI
MseI MspI MwoI NciI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I