Restriction map for
pFN21AE3896
(Circular) (Six-base) MAPSORT of: pFN21AE3896.seq Check: 1500 from: 1 to: 5680
pFN21AE3896 5680 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 2826 3807 4180 1563
Size: 1263 981 373 3063
Fragments arranged by size:
3063 1263 981 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2871 3743 5127 5395 1632
Size: 1239 872 1384 268 1917
Fragments arranged by size:
1917 1384 1239 872 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2431 2509 3423 4995 2431
Size: 78 914 1572 3116
Fragments arranged by size:
3116 1572 914 78
ApaI G_GGCC'C
Cuts at: 2446 2446
Size: 5680
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2256 2989 3215 3367 5476 1493
Size: 763 733 226 152 2109 1697
Fragments arranged by size:
2109 1697 763 733 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2445 2976 3005 1870
Size: 73 502 531 29 4545
Fragments arranged by size:
4545 531 502 73 29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2592 2625 2592
Size: 33 5647
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2409 3001 4456 618
Size: 325 217 627 622 592 1455 1842
Fragments arranged by size:
1842 1455 627 622 592 325 217
BanII G_rGCy'C
Cuts at: 1391 2446 2999 1391
Size: 1055 553 4072
Fragments arranged by size:
4072 1055 553
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2426 2498 4291
Size: 107 122 71 631 605 754 72 1793
Cuts at: 4291 136
Size: 1525
Fragments arranged by size:
1793 1525 754 631 605 122 107 72
71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2446 3741 4897 1790
Size: 656 1295 1156 2573
Fragments arranged by size:
2573 1295 1156 656
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 4340 1239
Size: 850 2251 2579
Fragments arranged by size:
2579 2251 850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2123 3471 493
Size: 711 919 1348 2702
Fragments arranged by size:
2702 1348 919 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 1178
Size: 30 165 30 684 30 4741
Fragments arranged by size:
4741 684 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5680
BseYI C'CCAG_C
Cuts at: 2586 4883 5379 2586
Size: 2297 496 2887
Fragments arranged by size:
2887 2297 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 1163
Size: 573 708 4399
Fragments arranged by size:
4399 708 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2949 3741 3826 4897 2949
Size: 792 85 1071 3732
Fragments arranged by size:
3732 1071 792 85
BsmI GAATG_Cn'
Cuts at: 2116 3191 3284 2116
Size: 1075 93 4512
Fragments arranged by size:
4512 1075 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2622 2622
Size: 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2446 2949 2999 3741 3826 4897 1391
Size: 399 656 503 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 656 503 399 85 50
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 2311 3569 5299 2311
Size: 1258 1730 2692
Fragments arranged by size:
2692 1730 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3035 844
Size: 2191 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2710 3567 1394
Size: 429 887 857 3507
Fragments arranged by size:
3507 887 857 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 4175 4349 65
Size: 1183 879 2048 174 1396
Fragments arranged by size:
2048 1396 1183 879 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 4324 4511 1700
Size: 110 254 2260 187 2869
Fragments arranged by size:
2869 2260 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 2734 96
Size: 2638 3042
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 2509 794
Size: 1637 78 3965
Fragments arranged by size:
3965 1637 78
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2191 2582 3010 3377 3762 3779 5231 5317
Size: 219 391 428 367 385 17 1452 86
Cuts at: 5317 5329 5675 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 428 391 385 367 346 219
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2795 487
Size: 683 56 1569 3372
Fragments arranged by size:
3372 1569 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2325 2984 2325
Size: 659 5021
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2222 2272
Size: 54 1425 50 275 18 177 215 50
Cuts at: 2272 2301 3127 4016 5645 8
Size: 29 826 889 1629 43
Fragments arranged by size:
1629 1425 889 826 275 215 177 54
50 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 2405 2700 3610 1353
Size: 996 56 295 910 3423
Fragments arranged by size:
3423 996 910 295 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2871 3743 4340 5127 5395 1239
Size: 393 457 782 872 597 787 268 1524
Fragments arranged by size:
1524 872 787 782 597 457 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2428 2506 2428
Size: 78 5602
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 4827 1678
Size: 3149 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2567 2599 4321 4353 4395
Size: 32 135 32 995 32 1722 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 1722 995 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2303 10
Size: 54 1425 343 177 294 3387
Fragments arranged by size:
3387 1425 343 294 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 2942 3638 3997 4156 518
Size: 648 1530 246 696 359 159 2042
Fragments arranged by size:
2042 1530 696 648 359 246 159
MspA1I CmG'CkG
Cuts at: 1606 2071 3096 3773 4921 5166 1606
Size: 465 1025 677 1148 245 2120
Fragments arranged by size:
2120 1148 1025 677 465 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2366 3038 4583 1132
Size: 97 1137 672 1545 2229
Fragments arranged by size:
2229 1545 1137 672 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PspOMI G'GGCC_C
Cuts at: 2442 2442
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2514 3032 830
Size: 1684 518 3478
Fragments arranged by size:
3478 1684 518
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2071 2071
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SapI GCTCTTCn'nnn_
Cuts at: 2405 2700 2405
Size: 295 5385
SbfI CC_TGCA'GG
Cuts at: 2514 3032 2514
Size: 518 5162
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2510 3028 4163 4844 5035 826
Size: 219 1465 518 1135 681 191 1471
Fragments arranged by size:
1471 1465 1135 681 518 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 2447 3007 1872
Size: 575 560 4545
Fragments arranged by size:
4545 575 560
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2976 3784 4685 4947 820
Size: 197 926 1033 808 901 262 1553
Fragments arranged by size:
1553 1033 926 901 808 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SrfI GCCC'GGGC
Cuts at: 2447 2447
Size: 5680
SspI AAT'ATT
Cuts at: 5 52 2763 2925 3604 5
Size: 47 2711 162 679 2081
Fragments arranged by size:
2711 2081 679 162 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 3119 513
Size: 552 939 1115 3074
Fragments arranged by size:
3074 1115 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 3707 3724 3877 4062 4507 325
Size: 1255 647 1480 17 153 185 445 1498
Fragments arranged by size:
1498 1480 1255 647 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2734 3926 5638
Size: 274 80 33 51 1443 757 1192 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1712 1443 1192 757 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 5640 5672 2087
Size: 32 3521 32 2095
Fragments arranged by size:
3521 2095 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2232 2232
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 2976 1943
Size: 1033 4647
XmaI C'CCGG_G
Cuts at: 1870 2445 3005 1870
Size: 575 560 4545
Fragments arranged by size:
4545 575 560
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SwaI