Restriction map for
pFN21AE3892
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmFI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 13 GACGCC G[GA]^CG[CT]C
AocI 411 CCTGAGG CC^T[ACGT]AGG
AvaI 437 CTCGGG C^[CT]CG[GA]G
BfaI 181 CTAG C^TAG
BpmI 310 CTCCAG CTGGAG(16/14)
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaI 27 GAGACC GGTCTC(1/5)
BsiEI 258 CGATCG CG[GA][CT]^CG
BsmBI 8 GAGACG CGTCTC(1/5)
BsoBI 437 CTCGGG C^[CT]CG[GA]G
BspHI 170 TCATGA T^CATGA
BstEII 192 GGTCACC G^GT[ACGT]ACC
BstF5I 36 GGATG GGATG(2/0)
BstPI 192 GGTCACC G^GT[ACGT]ACC
Bsu36I 411 CCTGAGG CC^T[ACGT]AGG
DraII 329 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 237 CCACGG C^C[GA][CT]GG
EarI 396 GAAGAG CTCTTC(1/4)
Eco81I 411 CCTGAGG CC^T[ACGT]AGG
EcoNI 17 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 329 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 192 GGTCACC G^GT[ACGT]ACC
FokI 36 GGATG GGATG(9/13)
HaeIII 69 GGCC GG^CC
HgaI 13 GACGC GACGC(5/10)
Hin1I 13 GACGCC G[GA]^CG[CT]C
HphI 194 TCACC GGTGA(8/7)
Ksp632I 396 GAAGAG CTCTTC(1/4)
MaeI 181 CTAG C^TAG
MaeII 285 ACGT A^CGT
MslI 238 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 381 CAGCTG C[AC]G^C[GT]G
PpuMI 329 AGGACCC [GA]G^G[AT]CC[CT]
PstI 227 CTGCAG CTGCA^G
PvuI 258 CGATCG CGAT^CG
PvuII 381 CAGCTG CAG^CTG
RcaI 170 TCATGA T^CATGA
SfaNI 40 GATGC GCATC(5/9)
SfcI 227 CTGCAG C^T[GA][CT]AG
StuI 68 AGGCCT AGG^CCT
Tsp45I 193 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 155 AGCT AG^CT
382 AGCT
AvaII 330 GGACC G^G[AT]CC
355 GGACC
BcgI 12 CGACGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
75 CGATGATATTGC
BsmAI 8 GAGAC GTCTC(1/5)
27 GAGAC
BssKI 32 CCAGG ^CC[ACGT]GG
352 CCTGG
BstNI 32 CCAGG CC^[AT]GG
352 CCTGG
Cfr13I 330 GGACC G^G[ACGT]CC
355 GGACC
DpnII 259 GATC ^GATC
401 GATC
EcoRII 32 CCAGG ^CC[AT]GG
352 CCTGG
HinfI 273 GAATC G^A[ACGT]TC
317 GAATC
MboI 259 GATC ^GATC
401 GATC
MvaI 32 CCAGG CC^[AT]GG
352 CCTGG
NdeII 259 GATC ^GATC
401 GATC
NlaIV 330 GGACCC GG[ACGT]^[ACGT]CC
355 GGACCC
NspI 347 ACATGC [GA]CATG^[CT]
451 ACATGC
Sau3AI 259 GATC ^GATC
401 GATC
Sau96I 330 GGACC G^G[ACGT]CC
355 GGACC
ScrFI 32 CCAGG CC^[ACGT]GG
352 CCTGG
TaqI 74 TCGA T^CGA
261 TCGA
TfiI 273 GAATC G^A[AT]TC
317 GAATC
TthHB8I 74 TCGA T^CGA
261 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI DdeI
Fnu4HI ItaI MaeIII MboII MnlI
NlaIII TseI